Amino acid dipepetide frequency for Epulopiscium sp. Nuni2H_MBin001

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.9AlaAla: 4.9 ± 0.144
0.687AlaCys: 0.687 ± 0.033
4.05AlaAsp: 4.05 ± 0.087
4.052AlaGlu: 4.052 ± 0.088
2.55AlaPhe: 2.55 ± 0.069
4.37AlaGly: 4.37 ± 0.083
1.101AlaHis: 1.101 ± 0.043
6.501AlaIle: 6.501 ± 0.102
4.76AlaLys: 4.76 ± 0.091
6.53AlaLeu: 6.53 ± 0.111
1.923AlaMet: 1.923 ± 0.058
3.667AlaAsn: 3.667 ± 0.076
1.863AlaPro: 1.863 ± 0.058
3.103AlaGln: 3.103 ± 0.101
2.176AlaArg: 2.176 ± 0.063
4.059AlaSer: 4.059 ± 0.077
4.87AlaThr: 4.87 ± 0.143
4.958AlaVal: 4.958 ± 0.101
0.448AlaTrp: 0.448 ± 0.025
2.621AlaTyr: 2.621 ± 0.079
0.0AlaXaa: 0.0 ± 0.0
Cys
0.759CysAla: 0.759 ± 0.039
0.205CysCys: 0.205 ± 0.019
0.633CysAsp: 0.633 ± 0.028
0.722CysGlu: 0.722 ± 0.034
0.39CysPhe: 0.39 ± 0.024
0.904CysGly: 0.904 ± 0.04
0.204CysHis: 0.204 ± 0.021
1.031CysIle: 1.031 ± 0.043
0.694CysLys: 0.694 ± 0.038
0.89CysLeu: 0.89 ± 0.035
0.298CysMet: 0.298 ± 0.02
0.65CysAsn: 0.65 ± 0.033
0.352CysPro: 0.352 ± 0.021
0.293CysGln: 0.293 ± 0.021
0.318CysArg: 0.318 ± 0.022
0.718CysSer: 0.718 ± 0.033
0.602CysThr: 0.602 ± 0.03
0.83CysVal: 0.83 ± 0.04
0.057CysTrp: 0.057 ± 0.009
0.607CysTyr: 0.607 ± 0.053
0.0CysXaa: 0.0 ± 0.0
Asp
3.705AspAla: 3.705 ± 0.096
0.612AspCys: 0.612 ± 0.033
4.088AspAsp: 4.088 ± 0.195
5.241AspGlu: 5.241 ± 0.124
2.603AspPhe: 2.603 ± 0.067
4.087AspGly: 4.087 ± 0.109
0.642AspHis: 0.642 ± 0.033
6.407AspIle: 6.407 ± 0.1
4.347AspLys: 4.347 ± 0.092
5.224AspLeu: 5.224 ± 0.095
1.798AspMet: 1.798 ± 0.052
3.77AspAsn: 3.77 ± 0.092
1.518AspPro: 1.518 ± 0.062
1.008AspGln: 1.008 ± 0.037
1.763AspArg: 1.763 ± 0.052
3.317AspSer: 3.317 ± 0.096
3.786AspThr: 3.786 ± 0.093
4.453AspVal: 4.453 ± 0.105
0.55AspTrp: 0.55 ± 0.028
2.888AspTyr: 2.888 ± 0.066
0.0AspXaa: 0.0 ± 0.0
Glu
5.048GluAla: 5.048 ± 0.11
0.608GluCys: 0.608 ± 0.033
4.314GluAsp: 4.314 ± 0.153
5.308GluGlu: 5.308 ± 0.13
3.046GluPhe: 3.046 ± 0.068
3.671GluGly: 3.671 ± 0.09
1.375GluHis: 1.375 ± 0.055
6.484GluIle: 6.484 ± 0.114
3.909GluLys: 3.909 ± 0.102
7.324GluLeu: 7.324 ± 0.124
1.846GluMet: 1.846 ± 0.06
3.704GluAsn: 3.704 ± 0.068
1.834GluPro: 1.834 ± 0.058
3.344GluGln: 3.344 ± 0.077
2.207GluArg: 2.207 ± 0.06
3.134GluSer: 3.134 ± 0.067
3.501GluThr: 3.501 ± 0.087
5.37GluVal: 5.37 ± 0.096
0.556GluTrp: 0.556 ± 0.031
3.03GluTyr: 3.03 ± 0.065
0.0GluXaa: 0.0 ± 0.0
Phe
2.68PheAla: 2.68 ± 0.061
0.492PheCys: 0.492 ± 0.026
2.662PheAsp: 2.662 ± 0.063
2.821PheGlu: 2.821 ± 0.058
1.538PhePhe: 1.538 ± 0.054
2.675PheGly: 2.675 ± 0.064
0.582PheHis: 0.582 ± 0.027
3.75PheIle: 3.75 ± 0.086
2.713PheLys: 2.713 ± 0.075
3.182PheLeu: 3.182 ± 0.074
1.108PheMet: 1.108 ± 0.04
2.444PheAsn: 2.444 ± 0.055
1.082PhePro: 1.082 ± 0.042
0.964PheGln: 0.964 ± 0.033
1.279PheArg: 1.279 ± 0.045
2.971PheSer: 2.971 ± 0.057
2.427PheThr: 2.427 ± 0.068
2.886PheVal: 2.886 ± 0.066
0.298PheTrp: 0.298 ± 0.021
1.653PheTyr: 1.653 ± 0.055
0.0PheXaa: 0.0 ± 0.0
Gly
4.4GlyAla: 4.4 ± 0.112
0.869GlyCys: 0.869 ± 0.034
3.763GlyAsp: 3.763 ± 0.089
4.107GlyGlu: 4.107 ± 0.092
2.698GlyPhe: 2.698 ± 0.066
3.962GlyGly: 3.962 ± 0.092
1.144GlyHis: 1.144 ± 0.047
6.004GlyIle: 6.004 ± 0.103
3.834GlyLys: 3.834 ± 0.086
5.458GlyLeu: 5.458 ± 0.094
1.746GlyMet: 1.746 ± 0.051
2.962GlyAsn: 2.962 ± 0.078
1.278GlyPro: 1.278 ± 0.107
1.846GlyGln: 1.846 ± 0.052
2.008GlyArg: 2.008 ± 0.059
3.616GlySer: 3.616 ± 0.07
3.971GlyThr: 3.971 ± 0.104
5.122GlyVal: 5.122 ± 0.088
0.457GlyTrp: 0.457 ± 0.022
2.923GlyTyr: 2.923 ± 0.065
0.0GlyXaa: 0.0 ± 0.0
His
0.915HisAla: 0.915 ± 0.037
0.27HisCys: 0.27 ± 0.019
0.925HisAsp: 0.925 ± 0.046
1.013HisGlu: 1.013 ± 0.042
0.694HisPhe: 0.694 ± 0.032
1.031HisGly: 1.031 ± 0.036
0.357HisHis: 0.357 ± 0.026
1.554HisIle: 1.554 ± 0.045
1.144HisLys: 1.144 ± 0.045
1.483HisLeu: 1.483 ± 0.042
0.46HisMet: 0.46 ± 0.028
1.042HisAsn: 1.042 ± 0.053
0.705HisPro: 0.705 ± 0.032
0.547HisGln: 0.547 ± 0.027
0.517HisArg: 0.517 ± 0.025
1.033HisSer: 1.033 ± 0.039
0.997HisThr: 0.997 ± 0.039
1.031HisVal: 1.031 ± 0.041
0.148HisTrp: 0.148 ± 0.012
0.749HisTyr: 0.749 ± 0.035
0.0HisXaa: 0.0 ± 0.0
Ile
6.501IleAla: 6.501 ± 0.097
1.15IleCys: 1.15 ± 0.045
5.933IleAsp: 5.933 ± 0.099
6.761IleGlu: 6.761 ± 0.121
3.459IlePhe: 3.459 ± 0.081
5.593IleGly: 5.593 ± 0.093
1.162IleHis: 1.162 ± 0.045
7.713IleIle: 7.713 ± 0.13
5.685IleLys: 5.685 ± 0.103
7.544IleLeu: 7.544 ± 0.121
2.144IleMet: 2.144 ± 0.05
5.285IleAsn: 5.285 ± 0.086
2.995IlePro: 2.995 ± 0.07
2.556IleGln: 2.556 ± 0.068
2.816IleArg: 2.816 ± 0.069
6.282IleSer: 6.282 ± 0.121
5.704IleThr: 5.704 ± 0.104
6.321IleVal: 6.321 ± 0.109
0.518IleTrp: 0.518 ± 0.027
3.454IleTyr: 3.454 ± 0.066
0.0IleXaa: 0.0 ± 0.0
Lys
4.208LysAla: 4.208 ± 0.114
0.597LysCys: 0.597 ± 0.031
3.925LysAsp: 3.925 ± 0.075
4.829LysGlu: 4.829 ± 0.102
2.504LysPhe: 2.504 ± 0.062
3.496LysGly: 3.496 ± 0.079
1.219LysHis: 1.219 ± 0.042
5.181LysIle: 5.181 ± 0.104
4.126LysLys: 4.126 ± 0.096
6.131LysLeu: 6.131 ± 0.116
1.733LysMet: 1.733 ± 0.053
3.238LysAsn: 3.238 ± 0.072
2.005LysPro: 2.005 ± 0.074
2.714LysGln: 2.714 ± 0.062
2.235LysArg: 2.235 ± 0.059
3.629LysSer: 3.629 ± 0.08
3.414LysThr: 3.414 ± 0.071
4.574LysVal: 4.574 ± 0.091
0.474LysTrp: 0.474 ± 0.027
2.886LysTyr: 2.886 ± 0.065
0.0LysXaa: 0.0 ± 0.0
Leu
6.635LeuAla: 6.635 ± 0.089
1.124LeuCys: 1.124 ± 0.048
5.868LeuAsp: 5.868 ± 0.097
6.845LeuGlu: 6.845 ± 0.115
3.431LeuPhe: 3.431 ± 0.089
6.084LeuGly: 6.084 ± 0.104
1.483LeuHis: 1.483 ± 0.05
7.208LeuIle: 7.208 ± 0.134
5.68LeuLys: 5.68 ± 0.103
7.824LeuLeu: 7.824 ± 0.135
2.346LeuMet: 2.346 ± 0.067
4.981LeuAsn: 4.981 ± 0.082
3.124LeuPro: 3.124 ± 0.073
3.117LeuGln: 3.117 ± 0.067
2.905LeuArg: 2.905 ± 0.066
6.367LeuSer: 6.367 ± 0.113
5.783LeuThr: 5.783 ± 0.1
6.027LeuVal: 6.027 ± 0.099
0.579LeuTrp: 0.579 ± 0.028
3.445LeuTyr: 3.445 ± 0.072
0.0LeuXaa: 0.0 ± 0.0
Met
2.004MetAla: 2.004 ± 0.055
0.284MetCys: 0.284 ± 0.019
1.539MetAsp: 1.539 ± 0.043
1.767MetGlu: 1.767 ± 0.049
1.024MetPhe: 1.024 ± 0.035
1.622MetGly: 1.622 ± 0.056
0.448MetHis: 0.448 ± 0.021
1.988MetIle: 1.988 ± 0.052
1.744MetLys: 1.744 ± 0.047
2.705MetLeu: 2.705 ± 0.07
0.763MetMet: 0.763 ± 0.037
1.325MetAsn: 1.325 ± 0.036
1.074MetPro: 1.074 ± 0.039
1.073MetGln: 1.073 ± 0.038
0.875MetArg: 0.875 ± 0.035
1.718MetSer: 1.718 ± 0.048
1.495MetThr: 1.495 ± 0.045
1.883MetVal: 1.883 ± 0.051
0.198MetTrp: 0.198 ± 0.017
1.034MetTyr: 1.034 ± 0.038
0.0MetXaa: 0.0 ± 0.0
Asn
3.462AsnAla: 3.462 ± 0.09
0.647AsnCys: 0.647 ± 0.034
2.888AsnAsp: 2.888 ± 0.073
3.604AsnGlu: 3.604 ± 0.069
2.136AsnPhe: 2.136 ± 0.053
3.399AsnGly: 3.399 ± 0.086
0.852AsnHis: 0.852 ± 0.037
5.441AsnIle: 5.441 ± 0.1
3.752AsnLys: 3.752 ± 0.088
5.358AsnLeu: 5.358 ± 0.106
1.566AsnMet: 1.566 ± 0.051
3.53AsnAsn: 3.53 ± 0.111
2.15AsnPro: 2.15 ± 0.065
1.879AsnGln: 1.879 ± 0.055
1.788AsnArg: 1.788 ± 0.054
3.469AsnSer: 3.469 ± 0.074
3.412AsnThr: 3.412 ± 0.083
3.761AsnVal: 3.761 ± 0.074
0.496AsnTrp: 0.496 ± 0.029
2.391AsnTyr: 2.391 ± 0.068
0.0AsnXaa: 0.0 ± 0.0
Pro
1.941ProAla: 1.941 ± 0.054
0.301ProCys: 0.301 ± 0.022
1.944ProAsp: 1.944 ± 0.086
2.242ProGlu: 2.242 ± 0.083
1.346ProPhe: 1.346 ± 0.047
1.463ProGly: 1.463 ± 0.052
0.593ProHis: 0.593 ± 0.028
2.8ProIle: 2.8 ± 0.069
1.837ProLys: 1.837 ± 0.062
2.557ProLeu: 2.557 ± 0.061
0.758ProMet: 0.758 ± 0.034
1.849ProAsn: 1.849 ± 0.056
0.838ProPro: 0.838 ± 0.047
1.297ProGln: 1.297 ± 0.048
0.809ProArg: 0.809 ± 0.034
2.005ProSer: 2.005 ± 0.059
2.26ProThr: 2.26 ± 0.063
2.282ProVal: 2.282 ± 0.059
0.216ProTrp: 0.216 ± 0.018
1.43ProTyr: 1.43 ± 0.055
0.0ProXaa: 0.0 ± 0.0
Gln
2.467GlnAla: 2.467 ± 0.075
0.342GlnCys: 0.342 ± 0.022
1.893GlnAsp: 1.893 ± 0.053
2.27GlnGlu: 2.27 ± 0.064
1.473GlnPhe: 1.473 ± 0.044
2.015GlnGly: 2.015 ± 0.104
0.675GlnHis: 0.675 ± 0.03
3.129GlnIle: 3.129 ± 0.074
1.988GlnLys: 1.988 ± 0.062
3.731GlnLeu: 3.731 ± 0.081
1.034GlnMet: 1.034 ± 0.039
1.832GlnAsn: 1.832 ± 0.051
0.885GlnPro: 0.885 ± 0.038
1.73GlnGln: 1.73 ± 0.056
1.108GlnArg: 1.108 ± 0.037
2.01GlnSer: 2.01 ± 0.056
2.018GlnThr: 2.018 ± 0.055
2.383GlnVal: 2.383 ± 0.058
0.327GlnTrp: 0.327 ± 0.021
1.57GlnTyr: 1.57 ± 0.057
0.0GlnXaa: 0.0 ± 0.0
Arg
2.198ArgAla: 2.198 ± 0.059
0.353ArgCys: 0.353 ± 0.024
1.869ArgAsp: 1.869 ± 0.052
2.365ArgGlu: 2.365 ± 0.065
1.305ArgPhe: 1.305 ± 0.044
1.862ArgGly: 1.862 ± 0.055
0.577ArgHis: 0.577 ± 0.032
2.69ArgIle: 2.69 ± 0.075
2.046ArgLys: 2.046 ± 0.064
3.154ArgLeu: 3.154 ± 0.074
1.0ArgMet: 1.0 ± 0.037
1.63ArgAsn: 1.63 ± 0.046
1.076ArgPro: 1.076 ± 0.05
1.226ArgGln: 1.226 ± 0.038
1.228ArgArg: 1.228 ± 0.042
1.568ArgSer: 1.568 ± 0.05
1.812ArgThr: 1.812 ± 0.051
2.263ArgVal: 2.263 ± 0.057
0.242ArgTrp: 0.242 ± 0.016
1.39ArgTyr: 1.39 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
4.256SerAla: 4.256 ± 0.088
0.634SerCys: 0.634 ± 0.029
3.652SerAsp: 3.652 ± 0.104
3.587SerGlu: 3.587 ± 0.079
2.513SerPhe: 2.513 ± 0.06
4.19SerGly: 4.19 ± 0.089
1.134SerHis: 1.134 ± 0.036
5.675SerIle: 5.675 ± 0.106
3.793SerLys: 3.793 ± 0.074
5.525SerLeu: 5.525 ± 0.101
1.653SerMet: 1.653 ± 0.058
3.518SerAsn: 3.518 ± 0.083
1.798SerPro: 1.798 ± 0.047
2.159SerGln: 2.159 ± 0.057
2.131SerArg: 2.131 ± 0.061
4.513SerSer: 4.513 ± 0.122
3.982SerThr: 3.982 ± 0.085
4.296SerVal: 4.296 ± 0.084
0.486SerTrp: 0.486 ± 0.024
2.731SerTyr: 2.731 ± 0.059
0.0SerXaa: 0.0 ± 0.0
Thr
4.548ThrAla: 4.548 ± 0.132
0.56ThrCys: 0.56 ± 0.026
3.671ThrAsp: 3.671 ± 0.108
3.527ThrGlu: 3.527 ± 0.078
2.597ThrPhe: 2.597 ± 0.067
3.995ThrGly: 3.995 ± 0.097
1.172ThrHis: 1.172 ± 0.042
5.729ThrIle: 5.729 ± 0.123
3.653ThrLys: 3.653 ± 0.081
5.798ThrLeu: 5.798 ± 0.116
1.381ThrMet: 1.381 ± 0.041
3.458ThrAsn: 3.458 ± 0.084
2.426ThrPro: 2.426 ± 0.071
2.42ThrGln: 2.42 ± 0.066
1.945ThrArg: 1.945 ± 0.054
3.879ThrSer: 3.879 ± 0.109
4.602ThrThr: 4.602 ± 0.164
4.275ThrVal: 4.275 ± 0.122
0.398ThrTrp: 0.398 ± 0.028
2.4ThrTyr: 2.4 ± 0.063
0.0ThrXaa: 0.0 ± 0.0
Val
5.547ValAla: 5.547 ± 0.096
0.861ValCys: 0.861 ± 0.037
4.896ValAsp: 4.896 ± 0.093
5.074ValGlu: 5.074 ± 0.089
2.653ValPhe: 2.653 ± 0.073
4.704ValGly: 4.704 ± 0.09
1.018ValHis: 1.018 ± 0.043
6.172ValIle: 6.172 ± 0.1
4.132ValLys: 4.132 ± 0.087
6.094ValLeu: 6.094 ± 0.107
1.815ValMet: 1.815 ± 0.05
3.84ValAsn: 3.84 ± 0.085
2.203ValPro: 2.203 ± 0.058
2.066ValGln: 2.066 ± 0.058
2.14ValArg: 2.14 ± 0.058
4.686ValSer: 4.686 ± 0.088
4.691ValThr: 4.691 ± 0.135
5.45ValVal: 5.45 ± 0.097
0.478ValTrp: 0.478 ± 0.025
2.721ValTyr: 2.721 ± 0.074
0.0ValXaa: 0.0 ± 0.0
Trp
0.552TrpAla: 0.552 ± 0.032
0.088TrpCys: 0.088 ± 0.012
0.466TrpAsp: 0.466 ± 0.027
0.514TrpGlu: 0.514 ± 0.026
0.402TrpPhe: 0.402 ± 0.038
0.474TrpGly: 0.474 ± 0.024
0.152TrpHis: 0.152 ± 0.014
0.596TrpIle: 0.596 ± 0.029
0.374TrpLys: 0.374 ± 0.02
0.667TrpLeu: 0.667 ± 0.033
0.17TrpMet: 0.17 ± 0.014
0.378TrpAsn: 0.378 ± 0.025
0.177TrpPro: 0.177 ± 0.014
0.267TrpGln: 0.267 ± 0.019
0.226TrpArg: 0.226 ± 0.019
0.458TrpSer: 0.458 ± 0.035
0.344TrpThr: 0.344 ± 0.022
0.469TrpVal: 0.469 ± 0.035
0.073TrpTrp: 0.073 ± 0.008
0.483TrpTyr: 0.483 ± 0.041
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.463TyrAla: 2.463 ± 0.062
0.473TyrCys: 0.473 ± 0.027
2.83TyrAsp: 2.83 ± 0.073
2.964TyrGlu: 2.964 ± 0.087
1.847TyrPhe: 1.847 ± 0.053
2.569TyrGly: 2.569 ± 0.059
0.726TyrHis: 0.726 ± 0.029
3.527TyrIle: 3.527 ± 0.081
2.908TyrLys: 2.908 ± 0.077
3.673TyrLeu: 3.673 ± 0.082
1.018TyrMet: 1.018 ± 0.036
2.88TyrAsn: 2.88 ± 0.066
1.383TyrPro: 1.383 ± 0.046
1.27TyrGln: 1.27 ± 0.039
1.38TyrArg: 1.38 ± 0.046
2.795TyrSer: 2.795 ± 0.058
2.763TyrThr: 2.763 ± 0.066
2.61TyrVal: 2.61 ± 0.057
0.33TyrTrp: 0.33 ± 0.022
2.016TyrTyr: 2.016 ± 0.069
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2185 proteins (784033 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski