Caulobacter phage Percy
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M3UKY6|A0A0M3UKY6_9CAUD Recombination DNA endonuclease OS=Caulobacter phage Percy OX=1701809 GN=CPT_Percy25 PE=4 SV=1
MM1 pKa = 7.46 LRR3 pKa = 11.84 ALGLNVDD10 pKa = 3.93 TADD13 pKa = 3.33 EE14 pKa = 4.59 FFPEE18 pKa = 4.04 LHH20 pKa = 7.06 DD21 pKa = 4.21 CRR23 pKa = 11.84 SEE25 pKa = 4.42 QGFQPQSEE33 pKa = 4.09 RR34 pKa = 11.84 DD35 pKa = 3.48 VMQSHH40 pKa = 7.29 GYY42 pKa = 11.04 DD43 pKa = 3.66 LMTPLDD49 pKa = 4.18 KK50 pKa = 10.97 FFEE53 pKa = 5.13 APMQMRR59 pKa = 11.84 MDD61 pKa = 3.69 YY62 pKa = 11.31 AEE64 pKa = 5.7 ALTCSDD70 pKa = 3.38 GG71 pKa = 3.63
Molecular weight: 8.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.212
IPC2_protein 4.202
IPC_protein 4.101
Toseland 3.91
ProMoST 4.253
Dawson 4.088
Bjellqvist 4.24
Wikipedia 4.012
Rodwell 3.935
Grimsley 3.821
Solomon 4.075
Lehninger 4.024
Nozaki 4.202
DTASelect 4.406
Thurlkill 3.961
EMBOSS 4.024
Sillero 4.228
Patrickios 3.389
IPC_peptide 4.075
IPC2_peptide 4.202
IPC2.peptide.svr19 4.131
Protein with the highest isoelectric point:
>tr|A0A0M3UL65|A0A0M3UL65_9CAUD Uncharacterized protein OS=Caulobacter phage Percy OX=1701809 GN=CPT_Percy29 PE=4 SV=1
MM1 pKa = 7.05 VPARR5 pKa = 11.84 SGRR8 pKa = 11.84 GSTVSHH14 pKa = 6.04 TRR16 pKa = 11.84 RR17 pKa = 11.84 STQSSGRR24 pKa = 11.84 TIRR27 pKa = 11.84 LSWPRR32 pKa = 11.84 STLTYY37 pKa = 9.25 RR38 pKa = 11.84 QPRR41 pKa = 11.84 RR42 pKa = 11.84 TASWPALWKK51 pKa = 10.49 PMLRR55 pKa = 11.84 TAA57 pKa = 4.96
Molecular weight: 6.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.422
IPC2_protein 11.111
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.208
Grimsley 12.691
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 11.945
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.119
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
14066
34
1308
255.7
28.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.588 ± 0.538
0.81 ± 0.145
6.029 ± 0.235
5.325 ± 0.422
3.363 ± 0.175
8.105 ± 0.405
1.877 ± 0.16
4.394 ± 0.146
5.41 ± 0.348
8.083 ± 0.287
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.766 ± 0.247
4.031 ± 0.235
4.294 ± 0.249
4.258 ± 0.327
5.588 ± 0.376
5.744 ± 0.402
6.548 ± 0.519
6.924 ± 0.243
1.493 ± 0.12
3.37 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here