Amino acid dipepetide frequency for Caulobacter phage Percy

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.642AlaAla: 15.642 ± 2.226
0.853AlaCys: 0.853 ± 0.268
7.11AlaAsp: 7.11 ± 0.602
6.47AlaGlu: 6.47 ± 0.72
3.199AlaPhe: 3.199 ± 0.439
10.451AlaGly: 10.451 ± 1.033
1.635AlaHis: 1.635 ± 0.405
4.977AlaIle: 4.977 ± 0.548
7.323AlaLys: 7.323 ± 0.849
9.314AlaLeu: 9.314 ± 0.899
3.484AlaMet: 3.484 ± 0.416
3.626AlaAsn: 3.626 ± 0.517
3.484AlaPro: 3.484 ± 0.398
5.332AlaGln: 5.332 ± 1.171
5.403AlaArg: 5.403 ± 0.919
6.754AlaSer: 6.754 ± 0.99
7.323AlaThr: 7.323 ± 0.72
8.034AlaVal: 8.034 ± 0.908
1.564AlaTrp: 1.564 ± 0.284
3.839AlaTyr: 3.839 ± 0.421
0.0AlaXaa: 0.0 ± 0.0
Cys
1.493CysAla: 1.493 ± 0.396
0.142CysCys: 0.142 ± 0.113
0.427CysAsp: 0.427 ± 0.162
0.213CysGlu: 0.213 ± 0.133
0.284CysPhe: 0.284 ± 0.125
0.142CysGly: 0.142 ± 0.108
0.142CysHis: 0.142 ± 0.133
0.64CysIle: 0.64 ± 0.238
0.355CysLys: 0.355 ± 0.142
0.569CysLeu: 0.569 ± 0.21
0.213CysMet: 0.213 ± 0.147
0.427CysAsn: 0.427 ± 0.153
0.64CysPro: 0.64 ± 0.24
0.142CysGln: 0.142 ± 0.127
0.284CysArg: 0.284 ± 0.14
0.498CysSer: 0.498 ± 0.225
0.498CysThr: 0.498 ± 0.203
0.64CysVal: 0.64 ± 0.222
0.142CysTrp: 0.142 ± 0.122
0.213CysTyr: 0.213 ± 0.134
0.0CysXaa: 0.0 ± 0.0
Asp
5.119AspAla: 5.119 ± 0.595
0.498AspCys: 0.498 ± 0.193
3.342AspAsp: 3.342 ± 0.526
3.626AspGlu: 3.626 ± 0.561
3.057AspPhe: 3.057 ± 0.424
4.764AspGly: 4.764 ± 0.701
0.995AspHis: 0.995 ± 0.303
3.484AspIle: 3.484 ± 0.402
3.982AspLys: 3.982 ± 0.68
4.337AspLeu: 4.337 ± 0.51
2.275AspMet: 2.275 ± 0.475
2.417AspAsn: 2.417 ± 0.384
4.195AspPro: 4.195 ± 0.668
2.986AspGln: 2.986 ± 0.42
3.342AspArg: 3.342 ± 0.465
2.488AspSer: 2.488 ± 0.461
3.768AspThr: 3.768 ± 0.716
2.915AspVal: 2.915 ± 0.477
0.569AspTrp: 0.569 ± 0.222
2.133AspTyr: 2.133 ± 0.396
0.0AspXaa: 0.0 ± 0.0
Glu
7.821GluAla: 7.821 ± 0.862
0.355GluCys: 0.355 ± 0.143
3.697GluAsp: 3.697 ± 0.59
2.702GluGlu: 2.702 ± 0.574
2.275GluPhe: 2.275 ± 0.417
4.479GluGly: 4.479 ± 0.63
1.493GluHis: 1.493 ± 0.356
2.56GluIle: 2.56 ± 0.553
3.199GluLys: 3.199 ± 0.543
3.768GluLeu: 3.768 ± 0.474
1.351GluMet: 1.351 ± 0.244
1.493GluAsn: 1.493 ± 0.307
1.635GluPro: 1.635 ± 0.352
2.631GluGln: 2.631 ± 0.421
3.768GluArg: 3.768 ± 0.61
1.635GluSer: 1.635 ± 0.333
2.417GluThr: 2.417 ± 0.39
3.199GluVal: 3.199 ± 0.443
0.924GluTrp: 0.924 ± 0.337
1.849GluTyr: 1.849 ± 0.35
0.0GluXaa: 0.0 ± 0.0
Phe
3.413PheAla: 3.413 ± 0.45
0.284PheCys: 0.284 ± 0.156
2.417PheAsp: 2.417 ± 0.449
1.564PheGlu: 1.564 ± 0.326
0.853PhePhe: 0.853 ± 0.257
2.346PheGly: 2.346 ± 0.467
0.64PheHis: 0.64 ± 0.196
1.138PheIle: 1.138 ± 0.29
1.564PheLys: 1.564 ± 0.279
3.199PheLeu: 3.199 ± 0.559
0.782PheMet: 0.782 ± 0.252
2.062PheAsn: 2.062 ± 0.342
0.924PhePro: 0.924 ± 0.284
1.849PheGln: 1.849 ± 0.336
2.133PheArg: 2.133 ± 0.379
1.92PheSer: 1.92 ± 0.409
2.488PheThr: 2.488 ± 0.42
2.773PheVal: 2.773 ± 0.567
0.355PheTrp: 0.355 ± 0.142
0.924PheTyr: 0.924 ± 0.234
0.0PheXaa: 0.0 ± 0.0
Gly
8.176GlyAla: 8.176 ± 0.993
0.427GlyCys: 0.427 ± 0.185
5.261GlyAsp: 5.261 ± 0.63
3.626GlyGlu: 3.626 ± 0.493
3.413GlyPhe: 3.413 ± 0.436
7.536GlyGly: 7.536 ± 0.881
1.351GlyHis: 1.351 ± 0.344
2.631GlyIle: 2.631 ± 0.415
5.19GlyLys: 5.19 ± 0.568
6.186GlyLeu: 6.186 ± 0.796
2.773GlyMet: 2.773 ± 0.447
3.199GlyAsn: 3.199 ± 0.425
2.417GlyPro: 2.417 ± 0.314
3.697GlyGln: 3.697 ± 0.462
5.048GlyArg: 5.048 ± 0.615
5.119GlySer: 5.119 ± 0.685
5.546GlyThr: 5.546 ± 1.156
5.475GlyVal: 5.475 ± 0.602
1.635GlyTrp: 1.635 ± 0.356
2.346GlyTyr: 2.346 ± 0.429
0.0GlyXaa: 0.0 ± 0.0
His
1.138HisAla: 1.138 ± 0.227
0.427HisCys: 0.427 ± 0.21
1.849HisAsp: 1.849 ± 0.373
1.066HisGlu: 1.066 ± 0.303
0.924HisPhe: 0.924 ± 0.251
1.635HisGly: 1.635 ± 0.381
0.355HisHis: 0.355 ± 0.156
0.64HisIle: 0.64 ± 0.26
0.924HisLys: 0.924 ± 0.246
1.351HisLeu: 1.351 ± 0.369
0.569HisMet: 0.569 ± 0.183
0.711HisAsn: 0.711 ± 0.207
0.924HisPro: 0.924 ± 0.271
0.711HisGln: 0.711 ± 0.193
1.066HisArg: 1.066 ± 0.362
0.995HisSer: 0.995 ± 0.227
1.066HisThr: 1.066 ± 0.321
1.209HisVal: 1.209 ± 0.328
0.284HisTrp: 0.284 ± 0.107
0.924HisTyr: 0.924 ± 0.248
0.0HisXaa: 0.0 ± 0.0
Ile
5.759IleAla: 5.759 ± 0.668
0.355IleCys: 0.355 ± 0.17
2.488IleAsp: 2.488 ± 0.365
2.702IleGlu: 2.702 ± 0.402
0.995IlePhe: 0.995 ± 0.225
3.91IleGly: 3.91 ± 0.584
0.64IleHis: 0.64 ± 0.297
2.417IleIle: 2.417 ± 0.393
2.56IleLys: 2.56 ± 0.42
2.844IleLeu: 2.844 ± 0.385
0.64IleMet: 0.64 ± 0.176
2.133IleAsn: 2.133 ± 0.384
2.275IlePro: 2.275 ± 0.33
1.564IleGln: 1.564 ± 0.254
2.56IleArg: 2.56 ± 0.467
2.346IleSer: 2.346 ± 0.325
2.702IleThr: 2.702 ± 0.392
3.626IleVal: 3.626 ± 0.384
0.427IleTrp: 0.427 ± 0.199
0.995IleTyr: 0.995 ± 0.311
0.0IleXaa: 0.0 ± 0.0
Lys
6.399LysAla: 6.399 ± 0.751
0.142LysCys: 0.142 ± 0.09
2.915LysAsp: 2.915 ± 0.349
3.271LysGlu: 3.271 ± 0.56
1.991LysPhe: 1.991 ± 0.344
3.413LysGly: 3.413 ± 0.516
1.564LysHis: 1.564 ± 0.325
1.991LysIle: 1.991 ± 0.339
1.635LysLys: 1.635 ± 0.451
4.692LysLeu: 4.692 ± 0.665
1.351LysMet: 1.351 ± 0.306
1.92LysAsn: 1.92 ± 0.287
2.844LysPro: 2.844 ± 0.444
2.275LysGln: 2.275 ± 0.397
2.844LysArg: 2.844 ± 0.433
3.057LysSer: 3.057 ± 0.535
4.195LysThr: 4.195 ± 0.752
4.621LysVal: 4.621 ± 0.581
1.066LysTrp: 1.066 ± 0.251
1.92LysTyr: 1.92 ± 0.323
0.0LysXaa: 0.0 ± 0.0
Leu
8.674LeuAla: 8.674 ± 0.671
1.066LeuCys: 1.066 ± 0.316
5.972LeuAsp: 5.972 ± 0.549
4.195LeuGlu: 4.195 ± 0.596
2.488LeuPhe: 2.488 ± 0.491
5.972LeuGly: 5.972 ± 0.821
1.777LeuHis: 1.777 ± 0.469
3.199LeuIle: 3.199 ± 0.549
3.91LeuLys: 3.91 ± 0.644
6.683LeuLeu: 6.683 ± 1.002
2.062LeuMet: 2.062 ± 0.512
3.91LeuAsn: 3.91 ± 0.573
3.413LeuPro: 3.413 ± 0.461
3.839LeuGln: 3.839 ± 0.413
4.692LeuArg: 4.692 ± 0.554
4.977LeuSer: 4.977 ± 0.659
4.266LeuThr: 4.266 ± 0.43
6.186LeuVal: 6.186 ± 0.576
0.924LeuTrp: 0.924 ± 0.261
2.631LeuTyr: 2.631 ± 0.362
0.0LeuXaa: 0.0 ± 0.0
Met
3.413MetAla: 3.413 ± 0.508
0.213MetCys: 0.213 ± 0.125
1.493MetAsp: 1.493 ± 0.327
1.422MetGlu: 1.422 ± 0.31
0.782MetPhe: 0.782 ± 0.212
1.635MetGly: 1.635 ± 0.351
0.569MetHis: 0.569 ± 0.204
0.569MetIle: 0.569 ± 0.199
1.422MetLys: 1.422 ± 0.291
2.915MetLeu: 2.915 ± 0.516
0.498MetMet: 0.498 ± 0.203
1.138MetAsn: 1.138 ± 0.291
1.138MetPro: 1.138 ± 0.234
1.564MetGln: 1.564 ± 0.366
1.066MetArg: 1.066 ± 0.241
2.275MetSer: 2.275 ± 0.375
2.56MetThr: 2.56 ± 0.534
1.706MetVal: 1.706 ± 0.341
0.284MetTrp: 0.284 ± 0.128
0.995MetTyr: 0.995 ± 0.216
0.0MetXaa: 0.0 ± 0.0
Asn
3.768AsnAla: 3.768 ± 0.588
0.427AsnCys: 0.427 ± 0.157
1.564AsnAsp: 1.564 ± 0.342
1.849AsnGlu: 1.849 ± 0.374
1.422AsnPhe: 1.422 ± 0.242
3.342AsnGly: 3.342 ± 0.511
0.569AsnHis: 0.569 ± 0.182
1.777AsnIle: 1.777 ± 0.229
1.706AsnLys: 1.706 ± 0.28
3.199AsnLeu: 3.199 ± 0.354
0.853AsnMet: 0.853 ± 0.261
2.275AsnAsn: 2.275 ± 0.44
2.702AsnPro: 2.702 ± 0.445
1.422AsnGln: 1.422 ± 0.396
2.346AsnArg: 2.346 ± 0.415
2.844AsnSer: 2.844 ± 0.49
3.342AsnThr: 3.342 ± 0.439
2.56AsnVal: 2.56 ± 0.414
0.498AsnTrp: 0.498 ± 0.162
1.849AsnTyr: 1.849 ± 0.385
0.0AsnXaa: 0.0 ± 0.0
Pro
3.91ProAla: 3.91 ± 0.45
0.569ProCys: 0.569 ± 0.212
2.773ProAsp: 2.773 ± 0.536
3.342ProGlu: 3.342 ± 0.447
1.209ProPhe: 1.209 ± 0.216
3.982ProGly: 3.982 ± 0.51
0.782ProHis: 0.782 ± 0.208
1.92ProIle: 1.92 ± 0.388
2.417ProLys: 2.417 ± 0.374
2.915ProLeu: 2.915 ± 0.596
1.28ProMet: 1.28 ± 0.254
1.991ProAsn: 1.991 ± 0.289
1.991ProPro: 1.991 ± 0.47
1.493ProGln: 1.493 ± 0.236
1.92ProArg: 1.92 ± 0.354
3.199ProSer: 3.199 ± 0.533
1.92ProThr: 1.92 ± 0.513
3.697ProVal: 3.697 ± 0.482
0.569ProTrp: 0.569 ± 0.167
1.066ProTyr: 1.066 ± 0.314
0.0ProXaa: 0.0 ± 0.0
Gln
6.399GlnAla: 6.399 ± 0.955
0.071GlnCys: 0.071 ± 0.075
2.133GlnAsp: 2.133 ± 0.389
1.849GlnGlu: 1.849 ± 0.381
1.351GlnPhe: 1.351 ± 0.293
3.271GlnGly: 3.271 ± 0.36
0.782GlnHis: 0.782 ± 0.273
2.133GlnIle: 2.133 ± 0.469
1.849GlnLys: 1.849 ± 0.407
2.702GlnLeu: 2.702 ± 0.446
1.92GlnMet: 1.92 ± 0.332
1.28GlnAsn: 1.28 ± 0.527
1.351GlnPro: 1.351 ± 0.347
2.417GlnGln: 2.417 ± 0.819
2.915GlnArg: 2.915 ± 0.436
2.488GlnSer: 2.488 ± 0.403
2.346GlnThr: 2.346 ± 0.512
3.271GlnVal: 3.271 ± 0.545
0.569GlnTrp: 0.569 ± 0.197
1.493GlnTyr: 1.493 ± 0.405
0.0GlnXaa: 0.0 ± 0.0
Arg
5.901ArgAla: 5.901 ± 0.771
0.711ArgCys: 0.711 ± 0.258
3.199ArgAsp: 3.199 ± 0.483
3.271ArgGlu: 3.271 ± 0.552
1.777ArgPhe: 1.777 ± 0.329
3.697ArgGly: 3.697 ± 0.605
1.138ArgHis: 1.138 ± 0.398
2.488ArgIle: 2.488 ± 0.495
3.128ArgLys: 3.128 ± 0.525
6.043ArgLeu: 6.043 ± 0.736
2.275ArgMet: 2.275 ± 0.467
2.488ArgAsn: 2.488 ± 0.385
2.346ArgPro: 2.346 ± 0.416
1.635ArgGln: 1.635 ± 0.399
3.555ArgArg: 3.555 ± 0.707
2.56ArgSer: 2.56 ± 0.469
3.626ArgThr: 3.626 ± 0.635
3.413ArgVal: 3.413 ± 0.549
0.853ArgTrp: 0.853 ± 0.273
1.777ArgTyr: 1.777 ± 0.394
0.0ArgXaa: 0.0 ± 0.0
Ser
7.181SerAla: 7.181 ± 0.808
0.427SerCys: 0.427 ± 0.183
2.915SerAsp: 2.915 ± 0.535
2.488SerGlu: 2.488 ± 0.452
1.706SerPhe: 1.706 ± 0.392
5.332SerGly: 5.332 ± 0.796
0.995SerHis: 0.995 ± 0.338
2.915SerIle: 2.915 ± 0.491
3.91SerLys: 3.91 ± 0.573
5.048SerLeu: 5.048 ± 0.512
1.138SerMet: 1.138 ± 0.234
2.204SerAsn: 2.204 ± 0.516
2.488SerPro: 2.488 ± 0.472
1.635SerGln: 1.635 ± 0.294
3.342SerArg: 3.342 ± 0.531
2.702SerSer: 2.702 ± 0.592
4.053SerThr: 4.053 ± 0.697
3.555SerVal: 3.555 ± 0.554
0.995SerTrp: 0.995 ± 0.313
2.417SerTyr: 2.417 ± 0.633
0.0SerXaa: 0.0 ± 0.0
Thr
9.172ThrAla: 9.172 ± 1.327
0.213ThrCys: 0.213 ± 0.131
2.915ThrAsp: 2.915 ± 0.479
2.986ThrGlu: 2.986 ± 0.436
1.635ThrPhe: 1.635 ± 0.413
5.688ThrGly: 5.688 ± 0.748
1.138ThrHis: 1.138 ± 0.276
3.484ThrIle: 3.484 ± 0.645
3.342ThrLys: 3.342 ± 0.454
5.688ThrLeu: 5.688 ± 0.614
1.422ThrMet: 1.422 ± 0.276
2.062ThrAsn: 2.062 ± 0.538
3.484ThrPro: 3.484 ± 0.461
2.417ThrGln: 2.417 ± 0.327
2.631ThrArg: 2.631 ± 0.377
4.053ThrSer: 4.053 ± 0.74
5.119ThrThr: 5.119 ± 1.106
4.479ThrVal: 4.479 ± 0.609
1.351ThrTrp: 1.351 ± 0.273
2.204ThrTyr: 2.204 ± 0.468
0.0ThrXaa: 0.0 ± 0.0
Val
7.039ValAla: 7.039 ± 0.893
0.498ValCys: 0.498 ± 0.23
3.91ValAsp: 3.91 ± 0.404
4.124ValGlu: 4.124 ± 0.691
2.133ValPhe: 2.133 ± 0.347
6.043ValGly: 6.043 ± 0.78
1.564ValHis: 1.564 ± 0.355
2.488ValIle: 2.488 ± 0.351
3.199ValLys: 3.199 ± 0.656
5.119ValLeu: 5.119 ± 0.566
1.849ValMet: 1.849 ± 0.318
3.128ValAsn: 3.128 ± 0.464
2.702ValPro: 2.702 ± 0.47
3.271ValGln: 3.271 ± 0.587
4.266ValArg: 4.266 ± 0.55
4.479ValSer: 4.479 ± 0.613
4.977ValThr: 4.977 ± 0.93
4.764ValVal: 4.764 ± 0.676
1.28ValTrp: 1.28 ± 0.228
2.417ValTyr: 2.417 ± 0.435
0.0ValXaa: 0.0 ± 0.0
Trp
1.777TrpAla: 1.777 ± 0.305
0.071TrpCys: 0.071 ± 0.072
1.138TrpAsp: 1.138 ± 0.237
0.853TrpGlu: 0.853 ± 0.248
0.853TrpPhe: 0.853 ± 0.287
0.569TrpGly: 0.569 ± 0.221
0.213TrpHis: 0.213 ± 0.123
0.427TrpIle: 0.427 ± 0.134
0.711TrpLys: 0.711 ± 0.26
1.351TrpLeu: 1.351 ± 0.293
0.427TrpMet: 0.427 ± 0.137
0.427TrpAsn: 0.427 ± 0.179
0.853TrpPro: 0.853 ± 0.26
0.427TrpGln: 0.427 ± 0.166
0.355TrpArg: 0.355 ± 0.146
0.924TrpSer: 0.924 ± 0.212
1.138TrpThr: 1.138 ± 0.404
1.493TrpVal: 1.493 ± 0.332
0.284TrpTrp: 0.284 ± 0.176
0.64TrpTyr: 0.64 ± 0.207
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.697TyrAla: 3.697 ± 0.451
0.355TyrCys: 0.355 ± 0.139
2.773TyrAsp: 2.773 ± 0.347
1.635TyrGlu: 1.635 ± 0.297
1.28TyrPhe: 1.28 ± 0.393
2.844TyrGly: 2.844 ± 0.463
0.427TyrHis: 0.427 ± 0.162
2.062TyrIle: 2.062 ± 0.406
1.777TyrLys: 1.777 ± 0.382
2.915TyrLeu: 2.915 ± 0.522
0.427TyrMet: 0.427 ± 0.214
1.422TyrAsn: 1.422 ± 0.287
1.138TyrPro: 1.138 ± 0.262
1.28TyrGln: 1.28 ± 0.29
2.346TyrArg: 2.346 ± 0.39
2.133TyrSer: 2.133 ± 0.441
2.133TyrThr: 2.133 ± 0.424
1.635TyrVal: 1.635 ± 0.292
0.355TyrTrp: 0.355 ± 0.17
1.066TyrTyr: 1.066 ± 0.279
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (14066 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski