Bifidobacterium eulemuris
Average proteome isoelectric point is 5.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2326 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A261GDI2|A0A261GDI2_9BIFI Uncharacterized protein OS=Bifidobacterium eulemuris OX=1765219 GN=BEUL_0719 PE=4 SV=1
MM1 pKa = 7.51 KK2 pKa = 10.12 RR3 pKa = 11.84 GKK5 pKa = 8.58 LTAVVAFTVSAMLALSGCGGSDD27 pKa = 3.16 TATDD31 pKa = 3.41 AVDD34 pKa = 3.35 NGEE37 pKa = 4.65 EE38 pKa = 4.03 ITLDD42 pKa = 3.7 VYY44 pKa = 11.81 DD45 pKa = 5.59 DD46 pKa = 3.7 FANYY50 pKa = 10.25 AGEE53 pKa = 4.15 QKK55 pKa = 10.7 GWFAQVIKK63 pKa = 10.94 DD64 pKa = 3.64 KK65 pKa = 11.38 FNIKK69 pKa = 10.49 LNIIAPNVSGGGSTLFDD86 pKa = 3.35 TRR88 pKa = 11.84 SAAGDD93 pKa = 3.52 LGDD96 pKa = 3.65 IVLYY100 pKa = 10.25 NANNHH105 pKa = 4.97 STADD109 pKa = 4.18 LIKK112 pKa = 10.65 AGLIADD118 pKa = 4.06 MTPYY122 pKa = 10.82 LDD124 pKa = 3.67 DD125 pKa = 4.34 AEE127 pKa = 4.7 YY128 pKa = 10.4 IKK130 pKa = 10.56 QYY132 pKa = 11.36 SSATDD137 pKa = 3.59 SINEE141 pKa = 3.95 VAGQEE146 pKa = 4.15 SGVWGIPGSVSAQSPEE162 pKa = 3.94 TSSEE166 pKa = 3.97 GNEE169 pKa = 4.08 PTFGPYY175 pKa = 9.36 IRR177 pKa = 11.84 WDD179 pKa = 3.51 YY180 pKa = 9.88 YY181 pKa = 11.74 AEE183 pKa = 3.98 IGYY186 pKa = 9.04 PEE188 pKa = 4.75 IKK190 pKa = 10.88 DD191 pKa = 3.6 MDD193 pKa = 4.53 DD194 pKa = 3.85 LLDD197 pKa = 3.71 VLEE200 pKa = 4.33 QMQEE204 pKa = 3.68 QARR207 pKa = 11.84 ADD209 pKa = 3.63 TGSTDD214 pKa = 3.27 VYY216 pKa = 11.22 ALSLFKK222 pKa = 10.93 DD223 pKa = 3.2 WDD225 pKa = 4.07 DD226 pKa = 4.93 NMMNNAKK233 pKa = 9.96 QPTCFYY239 pKa = 10.68 GFDD242 pKa = 3.32 EE243 pKa = 4.63 MGFVLAKK250 pKa = 10.47 ADD252 pKa = 3.53 GSDD255 pKa = 3.71 YY256 pKa = 11.3 EE257 pKa = 4.6 DD258 pKa = 3.55 VSEE261 pKa = 4.38 EE262 pKa = 3.98 GGIYY266 pKa = 10.11 EE267 pKa = 3.98 QTLEE271 pKa = 4.56 FFNQAYY277 pKa = 9.53 QRR279 pKa = 11.84 GLVDD283 pKa = 4.55 PEE285 pKa = 5.06 SSTQSYY291 pKa = 8.29 DD292 pKa = 2.94 TMYY295 pKa = 11.42 AKK297 pKa = 10.49 YY298 pKa = 10.17 QEE300 pKa = 4.49 GNVLFSFWPWLGQAAYY316 pKa = 7.96 NTTEE320 pKa = 3.81 NKK322 pKa = 10.28 AAGKK326 pKa = 10.63 GFMLAPLEE334 pKa = 4.24 DD335 pKa = 3.81 MQIFSYY341 pKa = 10.71 GATPNGGTYY350 pKa = 10.5 VIALGAKK357 pKa = 9.46 AEE359 pKa = 4.06 YY360 pKa = 9.76 KK361 pKa = 9.8 QRR363 pKa = 11.84 CVDD366 pKa = 4.37 FINWLYY372 pKa = 10.9 SPEE375 pKa = 4.43 GIYY378 pKa = 10.72 ASGGQTSGSSAGVEE392 pKa = 4.01 GLTWEE397 pKa = 4.78 LNDD400 pKa = 4.84 DD401 pKa = 4.32 GEE403 pKa = 4.64 PVLTDD408 pKa = 3.27 FGYY411 pKa = 9.62 QVKK414 pKa = 9.78 YY415 pKa = 10.91 GSGGTVPDD423 pKa = 3.99 EE424 pKa = 4.27 YY425 pKa = 11.53 GGGNYY430 pKa = 9.16 TDD432 pKa = 4.57 GVSALNFVTVLASDD446 pKa = 3.97 IDD448 pKa = 4.14 PEE450 pKa = 4.02 TGYY453 pKa = 11.29 AYY455 pKa = 10.06 DD456 pKa = 3.6 ASLWPSVIEE465 pKa = 4.38 SQFSTALDD473 pKa = 3.95 NDD475 pKa = 3.77 WSEE478 pKa = 4.31 HH479 pKa = 4.91 MGGAKK484 pKa = 8.84 TAMEE488 pKa = 4.04 YY489 pKa = 11.16 LEE491 pKa = 5.54 ANDD494 pKa = 5.17 KK495 pKa = 11.01 ILVAPGASFVKK506 pKa = 10.62 PEE508 pKa = 4.86 DD509 pKa = 4.0 DD510 pKa = 3.85 SQISTLRR517 pKa = 11.84 GQLKK521 pKa = 6.95 TTVVNASWQAVFASSDD537 pKa = 3.79 SEE539 pKa = 4.21 FQSILDD545 pKa = 3.64 GMRR548 pKa = 11.84 TDD550 pKa = 5.38 LEE552 pKa = 4.28 GLGYY556 pKa = 10.58 DD557 pKa = 3.4 QVLEE561 pKa = 4.03 VDD563 pKa = 4.0 MKK565 pKa = 10.95 NAQDD569 pKa = 3.02 QDD571 pKa = 3.49 AARR574 pKa = 11.84 KK575 pKa = 9.67 QIVEE579 pKa = 4.75 DD580 pKa = 3.9 YY581 pKa = 10.81 QSEE584 pKa = 4.26 NN585 pKa = 3.14
Molecular weight: 63.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.821
IPC_protein 3.859
Toseland 3.63
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.757
Rodwell 3.668
Grimsley 3.541
Solomon 3.834
Lehninger 3.783
Nozaki 3.935
DTASelect 4.177
Thurlkill 3.668
EMBOSS 3.757
Sillero 3.973
Patrickios 1.227
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.859
Protein with the highest isoelectric point:
>tr|A0A261G1F4|A0A261G1F4_9BIFI Sodium:proton antiporter OS=Bifidobacterium eulemuris OX=1765219 GN=BEUL_2004 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 4.82 MKK15 pKa = 9.64 HH16 pKa = 5.7 GFRR19 pKa = 11.84 LRR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 SGRR28 pKa = 11.84 AVINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.06 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLSAA44 pKa = 3.86
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2326
0
2326
844624
29
3136
363.1
39.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.154 ± 0.065
0.98 ± 0.018
6.76 ± 0.048
5.961 ± 0.046
3.488 ± 0.034
8.081 ± 0.04
1.99 ± 0.029
5.096 ± 0.043
3.288 ± 0.04
8.863 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.669 ± 0.025
3.202 ± 0.034
4.474 ± 0.034
3.263 ± 0.028
6.146 ± 0.056
6.15 ± 0.048
6.143 ± 0.045
7.963 ± 0.047
1.458 ± 0.021
2.866 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here