Amino acid dipepetide frequency for Bifidobacterium eulemuris

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.758AlaAla: 13.758 ± 0.172	1.192AlaCys: 1.192 ± 0.038	7.976AlaAsp: 7.976 ± 0.123	6.44AlaGlu: 6.44 ± 0.104	3.609AlaPhe: 3.609 ± 0.066	8.883AlaGly: 8.883 ± 0.12	2.051AlaHis: 2.051 ± 0.055	5.593AlaIle: 5.593 ± 0.094	4.072AlaLys: 4.072 ± 0.092	10.729AlaLeu: 10.729 ± 0.15	3.364AlaMet: 3.364 ± 0.06	3.304AlaAsn: 3.304 ± 0.074	3.949AlaPro: 3.949 ± 0.089	4.124AlaGln: 4.124 ± 0.071	6.576AlaArg: 6.576 ± 0.109	6.717AlaSer: 6.717 ± 0.099	6.094AlaThr: 6.094 ± 0.103	8.864AlaVal: 8.864 ± 0.122	1.507AlaTrp: 1.507 ± 0.044	2.741AlaTyr: 2.741 ± 0.058	0.0AlaXaa: 0.0 ± 0.0
Cys	1.214CysAla: 1.214 ± 0.038	0.15CysCys: 0.15 ± 0.013	0.671CysAsp: 0.671 ± 0.033	0.592CysGlu: 0.592 ± 0.029	0.358CysPhe: 0.358 ± 0.022	1.016CysGly: 1.016 ± 0.036	0.211CysHis: 0.211 ± 0.015	0.375CysIle: 0.375 ± 0.021	0.223CysLys: 0.223 ± 0.015	0.818CysLeu: 0.818 ± 0.035	0.289CysMet: 0.289 ± 0.02	0.255CysAsn: 0.255 ± 0.019	0.478CysPro: 0.478 ± 0.025	0.227CysGln: 0.227 ± 0.018	0.553CysArg: 0.553 ± 0.027	0.562CysSer: 0.562 ± 0.03	0.516CysThr: 0.516 ± 0.025	0.907CysVal: 0.907 ± 0.037	0.137CysTrp: 0.137 ± 0.012	0.249CysTyr: 0.249 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	7.851AspAla: 7.851 ± 0.1	0.579AspCys: 0.579 ± 0.027	4.925AspAsp: 4.925 ± 0.088	5.558AspGlu: 5.558 ± 0.093	2.271AspPhe: 2.271 ± 0.06	6.518AspGly: 6.518 ± 0.128	1.197AspHis: 1.197 ± 0.04	3.197AspIle: 3.197 ± 0.07	2.019AspLys: 2.019 ± 0.059	5.323AspLeu: 5.323 ± 0.08	1.803AspMet: 1.803 ± 0.05	1.782AspAsn: 1.782 ± 0.052	3.591AspPro: 3.591 ± 0.067	1.982AspGln: 1.982 ± 0.053	3.526AspArg: 3.526 ± 0.071	3.656AspSer: 3.656 ± 0.079	3.399AspThr: 3.399 ± 0.071	5.134AspVal: 5.134 ± 0.088	1.141AspTrp: 1.141 ± 0.041	2.152AspTyr: 2.152 ± 0.065	0.001AspXaa: 0.001 ± 0.001
Glu	6.524GluAla: 6.524 ± 0.102	0.508GluCys: 0.508 ± 0.023	3.342GluAsp: 3.342 ± 0.068	3.475GluGlu: 3.475 ± 0.072	1.905GluPhe: 1.905 ± 0.05	4.28GluGly: 4.28 ± 0.07	1.575GluHis: 1.575 ± 0.047	2.792GluIle: 2.792 ± 0.063	1.829GluLys: 1.829 ± 0.054	5.684GluLeu: 5.684 ± 0.083	1.462GluMet: 1.462 ± 0.041	2.08GluAsn: 2.08 ± 0.055	2.698GluPro: 2.698 ± 0.072	2.631GluGln: 2.631 ± 0.062	4.962GluArg: 4.962 ± 0.09	3.732GluSer: 3.732 ± 0.08	3.684GluThr: 3.684 ± 0.079	3.732GluVal: 3.732 ± 0.078	0.823GluTrp: 0.823 ± 0.03	1.893GluTyr: 1.893 ± 0.051	0.001GluXaa: 0.001 ± 0.001
Phe	4.194PheAla: 4.194 ± 0.082	0.39PheCys: 0.39 ± 0.022	2.759PheAsp: 2.759 ± 0.055	1.991PheGlu: 1.991 ± 0.058	1.296PhePhe: 1.296 ± 0.052	3.251PheGly: 3.251 ± 0.068	0.67PheHis: 0.67 ± 0.027	1.649PheIle: 1.649 ± 0.058	1.023PheLys: 1.023 ± 0.039	2.798PheLeu: 2.798 ± 0.072	0.766PheMet: 0.766 ± 0.037	1.221PheAsn: 1.221 ± 0.041	1.366PhePro: 1.366 ± 0.047	0.954PheGln: 0.954 ± 0.035	1.607PheArg: 1.607 ± 0.048	2.341PheSer: 2.341 ± 0.057	2.263PheThr: 2.263 ± 0.053	2.87PheVal: 2.87 ± 0.065	0.516PheTrp: 0.516 ± 0.027	0.958PheTyr: 0.958 ± 0.037	0.001PheXaa: 0.001 ± 0.001
Gly	7.56GlyAla: 7.56 ± 0.109	0.834GlyCys: 0.834 ± 0.033	5.078GlyAsp: 5.078 ± 0.096	5.325GlyGlu: 5.325 ± 0.094	3.282GlyPhe: 3.282 ± 0.066	6.541GlyGly: 6.541 ± 0.117	1.613GlyHis: 1.613 ± 0.043	4.421GlyIle: 4.421 ± 0.083	3.235GlyLys: 3.235 ± 0.063	7.174GlyLeu: 7.174 ± 0.107	2.35GlyMet: 2.35 ± 0.054	2.48GlyAsn: 2.48 ± 0.057	2.396GlyPro: 2.396 ± 0.053	2.484GlyGln: 2.484 ± 0.063	4.76GlyArg: 4.76 ± 0.088	5.091GlySer: 5.091 ± 0.093	5.067GlyThr: 5.067 ± 0.099	6.974GlyVal: 6.974 ± 0.106	1.287GlyTrp: 1.287 ± 0.042	2.856GlyTyr: 2.856 ± 0.062	0.001GlyXaa: 0.001 ± 0.001
His	2.129HisAla: 2.129 ± 0.053	0.194HisCys: 0.194 ± 0.016	1.484HisAsp: 1.484 ± 0.044	1.182HisGlu: 1.182 ± 0.036	0.575HisPhe: 0.575 ± 0.026	1.853HisGly: 1.853 ± 0.053	0.494HisHis: 0.494 ± 0.025	1.048HisIle: 1.048 ± 0.038	0.508HisLys: 0.508 ± 0.02	1.558HisLeu: 1.558 ± 0.041	0.535HisMet: 0.535 ± 0.026	0.594HisAsn: 0.594 ± 0.024	1.185HisPro: 1.185 ± 0.039	0.521HisGln: 0.521 ± 0.022	1.352HisArg: 1.352 ± 0.044	0.934HisSer: 0.934 ± 0.035	1.21HisThr: 1.21 ± 0.044	1.636HisVal: 1.636 ± 0.05	0.322HisTrp: 0.322 ± 0.02	0.585HisTyr: 0.585 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.588IleAla: 6.588 ± 0.102	0.597IleCys: 0.597 ± 0.026	3.869IleAsp: 3.869 ± 0.071	3.196IleGlu: 3.196 ± 0.077	1.409IlePhe: 1.409 ± 0.052	4.475IleGly: 4.475 ± 0.084	0.878IleHis: 0.878 ± 0.035	2.676IleIle: 2.676 ± 0.082	1.473IleLys: 1.473 ± 0.052	3.728IleLeu: 3.728 ± 0.086	1.089IleMet: 1.089 ± 0.047	1.687IleAsn: 1.687 ± 0.048	2.496IlePro: 2.496 ± 0.062	1.317IleGln: 1.317 ± 0.037	2.789IleArg: 2.789 ± 0.06	3.042IleSer: 3.042 ± 0.062	3.31IleThr: 3.31 ± 0.064	4.564IleVal: 4.564 ± 0.084	0.559IleTrp: 0.559 ± 0.025	1.215IleTyr: 1.215 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	3.956LysAla: 3.956 ± 0.084	0.168LysCys: 0.168 ± 0.015	2.008LysAsp: 2.008 ± 0.054	1.867LysGlu: 1.867 ± 0.052	0.857LysPhe: 0.857 ± 0.034	2.345LysGly: 2.345 ± 0.061	0.76LysHis: 0.76 ± 0.03	1.492LysIle: 1.492 ± 0.051	1.405LysLys: 1.405 ± 0.048	2.651LysLeu: 2.651 ± 0.069	0.768LysMet: 0.768 ± 0.034	1.19LysAsn: 1.19 ± 0.044	1.945LysPro: 1.945 ± 0.054	1.286LysGln: 1.286 ± 0.035	2.302LysArg: 2.302 ± 0.054	1.858LysSer: 1.858 ± 0.057	2.347LysThr: 2.347 ± 0.054	2.313LysVal: 2.313 ± 0.071	0.379LysTrp: 0.379 ± 0.023	0.987LysTyr: 0.987 ± 0.038	0.0LysXaa: 0.0 ± 0.0
Leu	10.69LeuAla: 10.69 ± 0.139	0.966LeuCys: 0.966 ± 0.038	6.225LeuAsp: 6.225 ± 0.091	4.735LeuGlu: 4.735 ± 0.075	3.25LeuPhe: 3.25 ± 0.072	6.976LeuGly: 6.976 ± 0.096	1.654LeuHis: 1.654 ± 0.052	4.635LeuIle: 4.635 ± 0.104	3.066LeuLys: 3.066 ± 0.062	7.88LeuLeu: 7.88 ± 0.125	2.329LeuMet: 2.329 ± 0.051	2.948LeuAsn: 2.948 ± 0.062	4.416LeuPro: 4.416 ± 0.076	2.245LeuGln: 2.245 ± 0.055	5.288LeuArg: 5.288 ± 0.092	5.605LeuSer: 5.605 ± 0.092	5.723LeuThr: 5.723 ± 0.088	6.788LeuVal: 6.788 ± 0.101	1.125LeuTrp: 1.125 ± 0.039	2.089LeuTyr: 2.089 ± 0.056	0.001LeuXaa: 0.001 ± 0.001
Met	2.92MetAla: 2.92 ± 0.058	0.244MetCys: 0.244 ± 0.018	1.397MetAsp: 1.397 ± 0.05	1.263MetGlu: 1.263 ± 0.036	0.939MetPhe: 0.939 ± 0.038	1.964MetGly: 1.964 ± 0.05	0.469MetHis: 0.469 ± 0.024	1.262MetIle: 1.262 ± 0.042	0.862MetLys: 0.862 ± 0.029	2.646MetLeu: 2.646 ± 0.06	0.767MetMet: 0.767 ± 0.034	0.902MetAsn: 0.902 ± 0.035	1.493MetPro: 1.493 ± 0.043	0.772MetGln: 0.772 ± 0.035	2.066MetArg: 2.066 ± 0.053	1.917MetSer: 1.917 ± 0.04	1.987MetThr: 1.987 ± 0.046	1.977MetVal: 1.977 ± 0.057	0.341MetTrp: 0.341 ± 0.019	0.507MetTyr: 0.507 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	3.68AsnAla: 3.68 ± 0.064	0.243AsnCys: 0.243 ± 0.017	2.119AsnAsp: 2.119 ± 0.054	1.815AsnGlu: 1.815 ± 0.053	0.957AsnPhe: 0.957 ± 0.033	3.056AsnGly: 3.056 ± 0.071	0.671AsnHis: 0.671 ± 0.032	1.656AsnIle: 1.656 ± 0.048	1.006AsnLys: 1.006 ± 0.035	2.606AsnLeu: 2.606 ± 0.051	0.771AsnMet: 0.771 ± 0.03	1.064AsnAsn: 1.064 ± 0.041	2.145AsnPro: 2.145 ± 0.048	1.015AsnGln: 1.015 ± 0.035	1.846AsnArg: 1.846 ± 0.054	1.631AsnSer: 1.631 ± 0.049	2.061AsnThr: 2.061 ± 0.058	2.3AsnVal: 2.3 ± 0.057	0.432AsnTrp: 0.432 ± 0.024	0.939AsnTyr: 0.939 ± 0.039	0.001AsnXaa: 0.001 ± 0.001
Pro	4.741ProAla: 4.741 ± 0.086	0.328ProCys: 0.328 ± 0.021	3.531ProAsp: 3.531 ± 0.071	3.18ProGlu: 3.18 ± 0.068	1.699ProPhe: 1.699 ± 0.046	3.347ProGly: 3.347 ± 0.059	0.91ProHis: 0.91 ± 0.042	2.12ProIle: 2.12 ± 0.055	1.495ProLys: 1.495 ± 0.047	3.698ProLeu: 3.698 ± 0.073	1.115ProMet: 1.115 ± 0.036	1.527ProAsn: 1.527 ± 0.048	1.233ProPro: 1.233 ± 0.045	1.721ProGln: 1.721 ± 0.05	2.297ProArg: 2.297 ± 0.065	2.98ProSer: 2.98 ± 0.062	2.889ProThr: 2.889 ± 0.065	3.787ProVal: 3.787 ± 0.069	0.739ProTrp: 0.739 ± 0.032	1.397ProTyr: 1.397 ± 0.046	0.005ProXaa: 0.005 ± 0.003
Gln	3.76GlnAla: 3.76 ± 0.074	0.266GlnCys: 0.266 ± 0.019	1.558GlnAsp: 1.558 ± 0.049	1.667GlnGlu: 1.667 ± 0.046	1.058GlnPhe: 1.058 ± 0.036	2.377GlnGly: 2.377 ± 0.06	0.658GlnHis: 0.658 ± 0.027	1.772GlnIle: 1.772 ± 0.049	0.869GlnLys: 0.869 ± 0.033	3.064GlnLeu: 3.064 ± 0.065	0.937GlnMet: 0.937 ± 0.032	1.032GlnAsn: 1.032 ± 0.042	1.498GlnPro: 1.498 ± 0.062	1.484GlnGln: 1.484 ± 0.054	2.292GlnArg: 2.292 ± 0.055	2.219GlnSer: 2.219 ± 0.059	2.008GlnThr: 2.008 ± 0.054	2.428GlnVal: 2.428 ± 0.055	0.629GlnTrp: 0.629 ± 0.029	1.056GlnTyr: 1.056 ± 0.039	0.001GlnXaa: 0.001 ± 0.001
Arg	5.739ArgAla: 5.739 ± 0.107	0.598ArgCys: 0.598 ± 0.034	3.783ArgAsp: 3.783 ± 0.075	4.03ArgGlu: 4.03 ± 0.073	2.463ArgPhe: 2.463 ± 0.058	3.935ArgGly: 3.935 ± 0.072	1.502ArgHis: 1.502 ± 0.049	3.437ArgIle: 3.437 ± 0.065	2.309ArgLys: 2.309 ± 0.058	5.83ArgLeu: 5.83 ± 0.113	1.991ArgMet: 1.991 ± 0.058	1.988ArgAsn: 1.988 ± 0.053	2.508ArgPro: 2.508 ± 0.055	2.183ArgGln: 2.183 ± 0.05	5.037ArgArg: 5.037 ± 0.105	3.24ArgSer: 3.24 ± 0.071	3.321ArgThr: 3.321 ± 0.068	4.616ArgVal: 4.616 ± 0.086	0.929ArgTrp: 0.929 ± 0.031	2.017ArgTyr: 2.017 ± 0.049	0.001ArgXaa: 0.001 ± 0.001
Ser	6.337SerAla: 6.337 ± 0.111	0.539SerCys: 0.539 ± 0.025	4.26SerAsp: 4.26 ± 0.082	3.249SerGlu: 3.249 ± 0.066	2.201SerPhe: 2.201 ± 0.054	5.651SerGly: 5.651 ± 0.114	1.206SerHis: 1.206 ± 0.042	3.188SerIle: 3.188 ± 0.063	1.887SerLys: 1.887 ± 0.052	5.378SerLeu: 5.378 ± 0.084	1.686SerMet: 1.686 ± 0.041	1.892SerAsn: 1.892 ± 0.048	2.547SerPro: 2.547 ± 0.068	2.21SerGln: 2.21 ± 0.054	3.467SerArg: 3.467 ± 0.071	4.372SerSer: 4.372 ± 0.119	3.83SerThr: 3.83 ± 0.083	4.853SerVal: 4.853 ± 0.091	0.926SerTrp: 0.926 ± 0.033	1.826SerTyr: 1.826 ± 0.057	0.0SerXaa: 0.0 ± 0.0
Thr	6.629ThrAla: 6.629 ± 0.119	0.51ThrCys: 0.51 ± 0.025	4.101ThrAsp: 4.101 ± 0.087	2.889ThrGlu: 2.889 ± 0.058	2.099ThrPhe: 2.099 ± 0.049	5.112ThrGly: 5.112 ± 0.11	1.197ThrHis: 1.197 ± 0.037	3.398ThrIle: 3.398 ± 0.054	1.835ThrLys: 1.835 ± 0.052	5.857ThrLeu: 5.857 ± 0.093	1.564ThrMet: 1.564 ± 0.045	1.958ThrAsn: 1.958 ± 0.055	3.223ThrPro: 3.223 ± 0.068	1.971ThrGln: 1.971 ± 0.052	3.204ThrArg: 3.204 ± 0.071	3.585ThrSer: 3.585 ± 0.075	3.945ThrThr: 3.945 ± 0.082	5.767ThrVal: 5.767 ± 0.122	0.831ThrTrp: 0.831 ± 0.032	1.757ThrTyr: 1.757 ± 0.051	0.0ThrXaa: 0.0 ± 0.0
Val	8.792ValAla: 8.792 ± 0.121	0.965ValCys: 0.965 ± 0.032	5.477ValAsp: 5.477 ± 0.088	4.746ValGlu: 4.746 ± 0.095	3.051ValPhe: 3.051 ± 0.067	5.594ValGly: 5.594 ± 0.096	1.266ValHis: 1.266 ± 0.032	4.271ValIle: 4.271 ± 0.086	2.495ValLys: 2.495 ± 0.06	7.136ValLeu: 7.136 ± 0.124	2.026ValMet: 2.026 ± 0.05	2.624ValAsn: 2.624 ± 0.056	3.792ValPro: 3.792 ± 0.067	1.892ValGln: 1.892 ± 0.051	4.645ValArg: 4.645 ± 0.076	5.386ValSer: 5.386 ± 0.096	5.206ValThr: 5.206 ± 0.097	7.066ValVal: 7.066 ± 0.124	1.186ValTrp: 1.186 ± 0.036	2.017ValTyr: 2.017 ± 0.049	0.002ValXaa: 0.002 ± 0.003
Trp	1.272TrpAla: 1.272 ± 0.04	0.184TrpCys: 0.184 ± 0.014	0.875TrpAsp: 0.875 ± 0.033	0.648TrpGlu: 0.648 ± 0.026	0.556TrpPhe: 0.556 ± 0.026	1.0TrpGly: 1.0 ± 0.034	0.347TrpHis: 0.347 ± 0.019	0.771TrpIle: 0.771 ± 0.029	0.497TrpLys: 0.497 ± 0.025	1.534TrpLeu: 1.534 ± 0.049	0.431TrpMet: 0.431 ± 0.021	0.631TrpAsn: 0.631 ± 0.027	0.541TrpPro: 0.541 ± 0.027	0.649TrpGln: 0.649 ± 0.027	1.09TrpArg: 1.09 ± 0.038	0.97TrpSer: 0.97 ± 0.035	0.89TrpThr: 0.89 ± 0.03	0.933TrpVal: 0.933 ± 0.032	0.323TrpTrp: 0.323 ± 0.018	0.438TrpTyr: 0.438 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.212TyrAla: 3.212 ± 0.07	0.347TyrCys: 0.347 ± 0.021	2.162TyrAsp: 2.162 ± 0.061	1.764TyrGlu: 1.764 ± 0.059	1.047TyrPhe: 1.047 ± 0.031	2.628TyrGly: 2.628 ± 0.073	0.569TyrHis: 0.569 ± 0.028	1.203TyrIle: 1.203 ± 0.037	0.775TyrLys: 0.775 ± 0.037	2.538TyrLeu: 2.538 ± 0.062	0.651TyrMet: 0.651 ± 0.025	0.855TyrAsn: 0.855 ± 0.039	1.24TyrPro: 1.24 ± 0.039	0.967TyrGln: 0.967 ± 0.038	1.802TyrArg: 1.802 ± 0.049	1.666TyrSer: 1.666 ± 0.041	1.682TyrThr: 1.682 ± 0.062	2.123TyrVal: 2.123 ± 0.053	0.446TyrTrp: 0.446 ± 0.024	0.986TyrTyr: 0.986 ± 0.041	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.004XaaAsp: 0.004 ± 0.003	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.004XaaLeu: 0.004 ± 0.003	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 2326 proteins (844624 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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