Campylobacter phage CP30A
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 162 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J9SIQ1|J9SIQ1_9CAUD Ribonucleoside-diphosphate reductase OS=Campylobacter phage CP30A OX=1229752 PE=3 SV=1
MM1 pKa = 7.29 KK2 pKa = 10.18 LDD4 pKa = 3.4 YY5 pKa = 10.68 RR6 pKa = 11.84 VWDD9 pKa = 4.09 DD10 pKa = 3.46 KK11 pKa = 11.72 NKK13 pKa = 10.3 KK14 pKa = 9.62 FYY16 pKa = 11.11 YY17 pKa = 10.01 NEE19 pKa = 3.87 NPIDD23 pKa = 4.12 GDD25 pKa = 3.86 VEE27 pKa = 4.89 LFTGYY32 pKa = 9.78 IDD34 pKa = 3.83 EE35 pKa = 5.0 NGNKK39 pKa = 9.29 IYY41 pKa = 10.87 AGDD44 pKa = 3.84 ILEE47 pKa = 4.31 HH48 pKa = 5.55 LTWVEE53 pKa = 4.03 EE54 pKa = 4.04 YY55 pKa = 10.16 MEE57 pKa = 5.3 DD58 pKa = 3.68 EE59 pKa = 4.01 AHH61 pKa = 6.91 EE62 pKa = 3.96 EE63 pKa = 4.0 LIYY66 pKa = 10.65 EE67 pKa = 4.44 IVCFDD72 pKa = 4.25 IKK74 pKa = 11.07 DD75 pKa = 3.65 GLYY78 pKa = 10.82 SKK80 pKa = 10.98 LSDD83 pKa = 4.32 GCCGWYY89 pKa = 10.08 FEE91 pKa = 5.89 HH92 pKa = 7.73 FMDD95 pKa = 5.94 DD96 pKa = 3.35 KK97 pKa = 11.36 NNKK100 pKa = 8.3 IKK102 pKa = 10.52 EE103 pKa = 3.96 MWLSGNIHH111 pKa = 6.63 EE112 pKa = 5.31 NMNLLKK118 pKa = 10.93 DD119 pKa = 3.83 EE120 pKa = 4.36 EE121 pKa = 4.35
Molecular weight: 14.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.101
IPC2_protein 4.279
IPC_protein 4.215
Toseland 4.05
ProMoST 4.304
Dawson 4.177
Bjellqvist 4.368
Wikipedia 4.062
Rodwell 4.05
Grimsley 3.961
Solomon 4.177
Lehninger 4.126
Nozaki 4.291
DTASelect 4.457
Thurlkill 4.062
EMBOSS 4.075
Sillero 4.329
Patrickios 2.74
IPC_peptide 4.177
IPC2_peptide 4.317
IPC2.peptide.svr19 4.247
Protein with the highest isoelectric point:
>tr|J9SPC0|J9SPC0_9CAUD Putative DNA repair and recombination protein OS=Campylobacter phage CP30A OX=1229752 PE=4 SV=1
MM1 pKa = 7.69 PPIKK5 pKa = 10.28 HH6 pKa = 5.99 MSVADD11 pKa = 5.29 RR12 pKa = 11.84 IAQKK16 pKa = 10.37 RR17 pKa = 11.84 YY18 pKa = 9.02 RR19 pKa = 11.84 KK20 pKa = 8.84 QPKK23 pKa = 9.0 VKK25 pKa = 10.09 RR26 pKa = 11.84 KK27 pKa = 9.97 LKK29 pKa = 10.22 IRR31 pKa = 11.84 AKK33 pKa = 10.15 KK34 pKa = 8.42 NAKK37 pKa = 9.87 APSEE41 pKa = 3.99 NMSWSSKK48 pKa = 8.72 KK49 pKa = 9.93 RR50 pKa = 11.84 GYY52 pKa = 8.83 VRR54 pKa = 11.84 KK55 pKa = 9.87 DD56 pKa = 2.95 PKK58 pKa = 10.58 LRR60 pKa = 11.84 RR61 pKa = 11.84 TMKK64 pKa = 10.55 LVAKK68 pKa = 10.31 LRR70 pKa = 11.84 RR71 pKa = 11.84 KK72 pKa = 9.51 SS73 pKa = 3.27
Molecular weight: 8.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.195
IPC2_protein 10.57
IPC_protein 11.798
Toseland 12.003
ProMoST 12.427
Dawson 12.003
Bjellqvist 11.959
Wikipedia 12.442
Rodwell 12.062
Grimsley 12.047
Solomon 12.457
Lehninger 12.354
Nozaki 11.989
DTASelect 11.959
Thurlkill 11.989
EMBOSS 12.471
Sillero 11.989
Patrickios 11.784
IPC_peptide 12.457
IPC2_peptide 11.418
IPC2.peptide.svr19 8.943
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
162
0
162
41206
50
1365
254.4
29.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.366 ± 0.172
1.832 ± 0.159
6.349 ± 0.151
6.829 ± 0.166
5.048 ± 0.158
4.948 ± 0.152
1.616 ± 0.103
9.251 ± 0.188
10.249 ± 0.231
8.732 ± 0.153
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.123 ± 0.069
8.615 ± 0.172
2.64 ± 0.092
2.597 ± 0.09
2.602 ± 0.103
6.902 ± 0.176
5.118 ± 0.181
5.072 ± 0.15
0.808 ± 0.049
5.303 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here