Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4515 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q2W7X2|Q2W7X2_MAGSA Membrane-fusion protein OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=amb1249 PE=3 SV=1
MM1 pKa = 6.87 PTCRR5 pKa = 11.84 PLGAGEE11 pKa = 4.75 SMTKK15 pKa = 9.31 TFAVASADD23 pKa = 3.34 GTATQNVTVTINGANDD39 pKa = 3.15 SATISGSTTGAVAEE53 pKa = 4.95 DD54 pKa = 4.03 GNGRR58 pKa = 11.84 ATGTLSVADD67 pKa = 3.78 VDD69 pKa = 4.11 HH70 pKa = 6.53 GQAHH74 pKa = 5.2 VTAQTVQTDD83 pKa = 3.0 QGTFSIGEE91 pKa = 3.92 NGQWTFQVNSANADD105 pKa = 3.59 VQALGAGEE113 pKa = 5.03 SMTKK117 pKa = 9.31 TFAVASADD125 pKa = 3.34 GTATQNVSVTITGSNDD141 pKa = 2.67 GPTVSGAVGLGHH153 pKa = 5.82 TAEE156 pKa = 4.84 DD157 pKa = 3.55 NSVTFTKK164 pKa = 10.74 AQLLANAHH172 pKa = 6.37 DD173 pKa = 4.27 TDD175 pKa = 3.57 AHH177 pKa = 6.13 DD178 pKa = 4.01 TLSVSNLSAAHH189 pKa = 6.45 GSIVDD194 pKa = 4.06 NGNGTYY200 pKa = 10.21 TFTPTEE206 pKa = 4.21 TFSGHH211 pKa = 5.42 VDD213 pKa = 3.07 VSYY216 pKa = 9.91 TVSDD220 pKa = 3.43 GHH222 pKa = 6.81 GGTTTGSAALDD233 pKa = 3.4 VDD235 pKa = 4.42 AVADD239 pKa = 4.18 RR240 pKa = 11.84 ATVSVAIGAPVEE252 pKa = 4.34 TPNGSFTVTNLDD264 pKa = 3.52 STASAGYY271 pKa = 9.45 NNTYY275 pKa = 10.16 GYY277 pKa = 11.44 YY278 pKa = 11.27 VMDD281 pKa = 5.13 DD282 pKa = 3.56 SGNPTSGGVIWSNVHH297 pKa = 6.46 ASPGASVTISGVDD310 pKa = 3.46 PDD312 pKa = 3.41 RR313 pKa = 11.84 VGFFLIPDD321 pKa = 4.4 GGSQNSGLANGAALTFSKK339 pKa = 10.55 DD340 pKa = 4.07 ASGNWQASDD349 pKa = 3.41 SAGHH353 pKa = 5.62 VLSGAGTKK361 pKa = 10.27 VLFDD365 pKa = 3.81 KK366 pKa = 11.12 SALNGDD372 pKa = 4.1 HH373 pKa = 6.32 MVHH376 pKa = 7.11 SEE378 pKa = 3.96 DD379 pKa = 3.51 TSAVSGNQNWEE390 pKa = 4.1 DD391 pKa = 3.52 LTGGGDD397 pKa = 3.57 RR398 pKa = 11.84 DD399 pKa = 4.01 YY400 pKa = 11.66 NDD402 pKa = 3.5 VNMSVTWGNAAPTATHH418 pKa = 7.28 PLTVSASFPDD428 pKa = 3.44 MDD430 pKa = 4.06 GSEE433 pKa = 3.91 GHH435 pKa = 6.33 AVKK438 pKa = 10.6 ISGLPSGSTLYY449 pKa = 10.81 QNGVALVAGADD460 pKa = 3.72 GSYY463 pKa = 10.96 SLNPAQLGGLSVKK476 pKa = 9.82 TPAGFNGDD484 pKa = 3.7 LNVNVTAVATDD495 pKa = 3.82 GTSVASSTASATVHH509 pKa = 7.41 DD510 pKa = 5.39 DD511 pKa = 3.89 LSNHH515 pKa = 6.18 GPDD518 pKa = 4.08 AGDD521 pKa = 3.24 ATSVHH526 pKa = 6.09 GAMGATITGAAGVTDD541 pKa = 3.37 SDD543 pKa = 4.35 GDD545 pKa = 3.97 HH546 pKa = 6.99 LSYY549 pKa = 11.23 AVGTGTSGPQHH560 pKa = 5.54 GTVVVNTDD568 pKa = 2.8 GTFTYY573 pKa = 9.57 TPSGNYY579 pKa = 10.03 SGTDD583 pKa = 3.1 TFKK586 pKa = 9.75 ITVSDD591 pKa = 3.67 GHH593 pKa = 6.66 GGTATEE599 pKa = 4.4 TVNVTVEE606 pKa = 4.04 NGAPTLTTANAATANDD622 pKa = 3.56 HH623 pKa = 6.24 SAVTGHH629 pKa = 5.31 VTGSDD634 pKa = 3.55 TPGDD638 pKa = 3.75 TLSYY642 pKa = 11.13 SLVDD646 pKa = 3.4 ANGNHH651 pKa = 5.94 VGSLVTDD658 pKa = 4.06 HH659 pKa = 6.28 GTVSINAATGDD670 pKa = 3.73 YY671 pKa = 10.47 TFTPNAANASLGVGASVTDD690 pKa = 3.56 SFKK693 pKa = 11.3 VVATDD698 pKa = 3.38 NHH700 pKa = 6.33 GADD703 pKa = 3.76 SNLGTVAVTVTGSNDD718 pKa = 3.22 GPVVTGVTGGTGNEE732 pKa = 4.66 SITSARR738 pKa = 11.84 SVVTGHH744 pKa = 6.88 ISAGDD749 pKa = 3.48 VDD751 pKa = 5.39 AGDD754 pKa = 3.74 TLSYY758 pKa = 11.11 SVTDD762 pKa = 3.55 NAAAGHH768 pKa = 6.56 HH769 pKa = 5.97 GTLAVNASGDD779 pKa = 3.82 YY780 pKa = 10.58 TFTASDD786 pKa = 4.32 SNWHH790 pKa = 5.83 GTDD793 pKa = 2.85 SFTVQVSDD801 pKa = 3.5 GHH803 pKa = 6.4 GGTVNQTVAITVNAQADD820 pKa = 3.66 AATINAQDD828 pKa = 3.77 ASMAAQSGATFTASGGYY845 pKa = 10.17 AEE847 pKa = 5.39 GGSGSDD853 pKa = 3.88 IINGSSANDD862 pKa = 3.38 ILYY865 pKa = 10.8 GDD867 pKa = 4.96 DD868 pKa = 3.71 PSGDD872 pKa = 3.76 TEE874 pKa = 4.31 VTVALNISAAAISGEE889 pKa = 4.35 SVSSITLSNLPGGAVLNHH907 pKa = 5.9 GHH909 pKa = 7.41 DD910 pKa = 3.93 NGDD913 pKa = 3.89 GTWTLAPGDD922 pKa = 4.08 LTGLTLTSNNGIGGTVDD939 pKa = 3.35 VAVTTLDD946 pKa = 3.62 GTSTAVTNSSFNVSFSGGFNDD967 pKa = 5.19 TITGGVGADD976 pKa = 3.33 TMYY979 pKa = 11.19 GGAGNDD985 pKa = 3.43 TFKK988 pKa = 11.29 VSGASEE994 pKa = 4.43 STGDD998 pKa = 3.69 IYY1000 pKa = 11.39 DD1001 pKa = 4.27 GGSGTDD1007 pKa = 3.29 TVLGSSGNDD1016 pKa = 3.33 VISVTSNLGNMRR1028 pKa = 11.84 SIEE1031 pKa = 4.27 VIDD1034 pKa = 4.43 GGAGTDD1040 pKa = 3.43 TLLAGSGNDD1049 pKa = 3.36 YY1050 pKa = 11.23 LDD1052 pKa = 3.97 FSGTTIRR1059 pKa = 11.84 GVEE1062 pKa = 4.3 VIDD1065 pKa = 3.54 TGAGNDD1071 pKa = 4.06 VITGSAGADD1080 pKa = 3.49 TILTGTGDD1088 pKa = 3.81 DD1089 pKa = 3.5 TVVAIGGQTAGDD1101 pKa = 4.14 VYY1103 pKa = 11.32 DD1104 pKa = 5.0 GGAGTDD1110 pKa = 3.59 TLNVDD1115 pKa = 4.67 LTPAQYY1121 pKa = 7.47 TTAVRR1126 pKa = 11.84 LEE1128 pKa = 4.15 LMSFASFVDD1137 pKa = 3.85 NPANAGKK1144 pKa = 10.47 SFTFQSLGGLKK1155 pKa = 8.96 ATNFEE1160 pKa = 4.41 DD1161 pKa = 4.74 LRR1163 pKa = 11.84 VTVDD1167 pKa = 3.86 GNDD1170 pKa = 2.86 VDD1172 pKa = 5.78 LNHH1175 pKa = 7.3 PPQVTSVEE1183 pKa = 4.38 SNYY1186 pKa = 9.72 TAGHH1190 pKa = 6.63 ADD1192 pKa = 3.71 GVVHH1196 pKa = 6.57 ATDD1199 pKa = 3.59 SDD1201 pKa = 4.36 GDD1203 pKa = 3.85 TMSYY1207 pKa = 11.14 SFGTNTNGTPITSMATAHH1225 pKa = 5.75 GTVTIDD1231 pKa = 3.04 SSTGEE1236 pKa = 4.04 YY1237 pKa = 10.52 HH1238 pKa = 6.04 FTASDD1243 pKa = 3.71 ANFKK1247 pKa = 9.65 GTDD1250 pKa = 3.38 TFSVTVKK1257 pKa = 10.67 DD1258 pKa = 3.96 GMGGTTTQKK1267 pKa = 10.72 VNLDD1271 pKa = 3.83 FNPGDD1276 pKa = 3.95 DD1277 pKa = 3.69 ATVVTGPTSLGGSDD1291 pKa = 4.99 EE1292 pKa = 4.16 DD1293 pKa = 3.78 TQTFIRR1299 pKa = 11.84 TDD1301 pKa = 3.29 QLLANATDD1309 pKa = 3.57 VDD1311 pKa = 4.21 NTLHH1315 pKa = 5.81 VANLSAVDD1323 pKa = 3.23 SHH1325 pKa = 6.37 GAVVGSFVHH1334 pKa = 6.91 AVDD1337 pKa = 4.58 ADD1339 pKa = 3.76 GHH1341 pKa = 6.43 DD1342 pKa = 4.31 GYY1344 pKa = 11.68 AFTPNANFAGDD1355 pKa = 3.39 VTVNYY1360 pKa = 10.64 DD1361 pKa = 3.14 VVTDD1365 pKa = 3.58 TGIATHH1371 pKa = 6.57 TSGSLHH1377 pKa = 6.24 VDD1379 pKa = 3.75 AVADD1383 pKa = 3.66 AATFDD1388 pKa = 3.74 ATIGSNGVSVAHH1400 pKa = 7.05 LGNDD1404 pKa = 3.35 SKK1406 pKa = 11.66 VVNLFVNDD1414 pKa = 4.05 TSSGTAGFDD1423 pKa = 2.91 IYY1425 pKa = 11.2 LGGTNIGHH1433 pKa = 4.93 YY1434 pKa = 9.09 TVGRR1438 pKa = 11.84 SYY1440 pKa = 11.51 SSDD1443 pKa = 2.9 KK1444 pKa = 11.1 SVSLTLTDD1452 pKa = 3.76 AQHH1455 pKa = 5.56 TQLLNGADD1463 pKa = 3.42 IKK1465 pKa = 11.05 IVDD1468 pKa = 3.59 NDD1470 pKa = 4.1 GYY1472 pKa = 8.98 NTRR1475 pKa = 11.84 DD1476 pKa = 3.45 VLVDD1480 pKa = 4.6 RR1481 pKa = 11.84 IQVDD1485 pKa = 3.55 GLTFQAEE1492 pKa = 4.08 NGILSGASTTSGYY1505 pKa = 11.01 YY1506 pKa = 9.87 GVSSEE1511 pKa = 4.43 TYY1513 pKa = 10.14 ARR1515 pKa = 11.84 LNNVGSSVTFDD1526 pKa = 3.53 FDD1528 pKa = 4.2 SSVSHH1533 pKa = 6.56 TSSGYY1538 pKa = 9.58 HH1539 pKa = 6.06 VDD1541 pKa = 4.55 LAGHH1545 pKa = 7.09 LNDD1548 pKa = 4.02 TDD1550 pKa = 3.89 GSEE1553 pKa = 4.15 TLSYY1557 pKa = 10.54 HH1558 pKa = 6.21 IDD1560 pKa = 3.34 AMTTGASLSYY1570 pKa = 9.79 TGNEE1574 pKa = 3.68 GTLVHH1579 pKa = 6.05 NTDD1582 pKa = 3.66 GSWDD1586 pKa = 3.83 FNVDD1590 pKa = 2.78 ASHH1593 pKa = 6.92 YY1594 pKa = 10.62 DD1595 pKa = 3.36 GAVSLNLNVANGTAGFDD1612 pKa = 3.38 VHH1614 pKa = 5.44 VTANATEE1621 pKa = 4.09 TSNLDD1626 pKa = 3.27 VAGVGDD1632 pKa = 4.08 TVHH1635 pKa = 7.28 CDD1637 pKa = 3.27 GAIGGAGAIPGITLVGNAGNDD1658 pKa = 4.06 DD1659 pKa = 4.0 IIGTNGNDD1667 pKa = 3.53 LLLGGKK1673 pKa = 9.8 GGATYY1678 pKa = 10.35 RR1679 pKa = 11.84 FSGGGCGSGGGWSIVQSDD1697 pKa = 4.1 TNDD1700 pKa = 3.46 VISAGAGDD1708 pKa = 3.87 DD1709 pKa = 4.15 VIYY1712 pKa = 11.07 GDD1714 pKa = 3.94 ARR1716 pKa = 11.84 LVNGNIQITGSGNDD1730 pKa = 3.48 VLDD1733 pKa = 4.34 GGSGNDD1739 pKa = 3.86 QIHH1742 pKa = 6.5 GGAGNDD1748 pKa = 3.89 TIIGGTGDD1756 pKa = 4.03 DD1757 pKa = 5.02 VMFGDD1762 pKa = 4.34 QGNDD1766 pKa = 2.65 TFLFDD1771 pKa = 4.72 FGFGHH1776 pKa = 7.17 DD1777 pKa = 3.76 VVDD1780 pKa = 4.93 GGRR1783 pKa = 11.84 GSNWTDD1789 pKa = 3.21 TLDD1792 pKa = 3.65 LTHH1795 pKa = 7.57 DD1796 pKa = 3.87 NQISSVNIEE1805 pKa = 4.54 GVSGWAVSVDD1815 pKa = 3.54 AQGHH1819 pKa = 5.35 HH1820 pKa = 6.2 VAQATNGAHH1829 pKa = 7.07 DD1830 pKa = 4.45 ANGTIVVTNHH1840 pKa = 7.04 DD1841 pKa = 4.03 GSQDD1845 pKa = 3.32 TIEE1848 pKa = 3.94 FHH1850 pKa = 6.8 NVEE1853 pKa = 4.2 KK1854 pKa = 11.09 VVWW1857 pKa = 3.61
Molecular weight: 185.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.808
IPC_protein 3.897
Toseland 3.643
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.884
Rodwell 3.706
Grimsley 3.554
Solomon 3.91
Lehninger 3.872
Nozaki 4.012
DTASelect 4.355
Thurlkill 3.706
EMBOSS 3.884
Sillero 4.024
Patrickios 1.367
IPC_peptide 3.897
IPC2_peptide 3.999
IPC2.peptide.svr19 3.894
Protein with the highest isoelectric point:
>tr|Q2W777|Q2W777_MAGSA Retron-type reverse transcriptase OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=amb1494 PE=4 SV=1
MM1 pKa = 7.53 IGALLRR7 pKa = 11.84 RR8 pKa = 11.84 LRR10 pKa = 11.84 RR11 pKa = 11.84 FFRR14 pKa = 11.84 PPGSPVVIQSVRR26 pKa = 11.84 GLSAPRR32 pKa = 11.84 HH33 pKa = 4.87 PQNN36 pKa = 3.88
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.462
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.281
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.047
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.153
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4515
0
4515
1451216
34
9529
321.4
34.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.644 ± 0.056
0.992 ± 0.016
5.671 ± 0.037
5.71 ± 0.05
3.392 ± 0.022
8.737 ± 0.054
2.135 ± 0.019
4.706 ± 0.027
3.51 ± 0.033
10.26 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.668 ± 0.02
2.489 ± 0.034
5.218 ± 0.049
3.093 ± 0.024
7.034 ± 0.07
5.468 ± 0.046
5.307 ± 0.089
7.613 ± 0.031
1.363 ± 0.016
1.99 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here