Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Magnetospirillum; Magnetospirillum magneticum

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4515 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q2W7X2|Q2W7X2_MAGSA Membrane-fusion protein OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=amb1249 PE=3 SV=1
MM1 pKa = 6.87PTCRR5 pKa = 11.84PLGAGEE11 pKa = 4.75SMTKK15 pKa = 9.31TFAVASADD23 pKa = 3.34GTATQNVTVTINGANDD39 pKa = 3.15SATISGSTTGAVAEE53 pKa = 4.95DD54 pKa = 4.03GNGRR58 pKa = 11.84ATGTLSVADD67 pKa = 3.78VDD69 pKa = 4.11HH70 pKa = 6.53GQAHH74 pKa = 5.2VTAQTVQTDD83 pKa = 3.0QGTFSIGEE91 pKa = 3.92NGQWTFQVNSANADD105 pKa = 3.59VQALGAGEE113 pKa = 5.03SMTKK117 pKa = 9.31TFAVASADD125 pKa = 3.34GTATQNVSVTITGSNDD141 pKa = 2.67GPTVSGAVGLGHH153 pKa = 5.82TAEE156 pKa = 4.84DD157 pKa = 3.55NSVTFTKK164 pKa = 10.74AQLLANAHH172 pKa = 6.37DD173 pKa = 4.27TDD175 pKa = 3.57AHH177 pKa = 6.13DD178 pKa = 4.01TLSVSNLSAAHH189 pKa = 6.45GSIVDD194 pKa = 4.06NGNGTYY200 pKa = 10.21TFTPTEE206 pKa = 4.21TFSGHH211 pKa = 5.42VDD213 pKa = 3.07VSYY216 pKa = 9.91TVSDD220 pKa = 3.43GHH222 pKa = 6.81GGTTTGSAALDD233 pKa = 3.4VDD235 pKa = 4.42AVADD239 pKa = 4.18RR240 pKa = 11.84ATVSVAIGAPVEE252 pKa = 4.34TPNGSFTVTNLDD264 pKa = 3.52STASAGYY271 pKa = 9.45NNTYY275 pKa = 10.16GYY277 pKa = 11.44YY278 pKa = 11.27VMDD281 pKa = 5.13DD282 pKa = 3.56SGNPTSGGVIWSNVHH297 pKa = 6.46ASPGASVTISGVDD310 pKa = 3.46PDD312 pKa = 3.41RR313 pKa = 11.84VGFFLIPDD321 pKa = 4.4GGSQNSGLANGAALTFSKK339 pKa = 10.55DD340 pKa = 4.07ASGNWQASDD349 pKa = 3.41SAGHH353 pKa = 5.62VLSGAGTKK361 pKa = 10.27VLFDD365 pKa = 3.81KK366 pKa = 11.12SALNGDD372 pKa = 4.1HH373 pKa = 6.32MVHH376 pKa = 7.11SEE378 pKa = 3.96DD379 pKa = 3.51TSAVSGNQNWEE390 pKa = 4.1DD391 pKa = 3.52LTGGGDD397 pKa = 3.57RR398 pKa = 11.84DD399 pKa = 4.01YY400 pKa = 11.66NDD402 pKa = 3.5VNMSVTWGNAAPTATHH418 pKa = 7.28PLTVSASFPDD428 pKa = 3.44MDD430 pKa = 4.06GSEE433 pKa = 3.91GHH435 pKa = 6.33AVKK438 pKa = 10.6ISGLPSGSTLYY449 pKa = 10.81QNGVALVAGADD460 pKa = 3.72GSYY463 pKa = 10.96SLNPAQLGGLSVKK476 pKa = 9.82TPAGFNGDD484 pKa = 3.7LNVNVTAVATDD495 pKa = 3.82GTSVASSTASATVHH509 pKa = 7.41DD510 pKa = 5.39DD511 pKa = 3.89LSNHH515 pKa = 6.18GPDD518 pKa = 4.08AGDD521 pKa = 3.24ATSVHH526 pKa = 6.09GAMGATITGAAGVTDD541 pKa = 3.37SDD543 pKa = 4.35GDD545 pKa = 3.97HH546 pKa = 6.99LSYY549 pKa = 11.23AVGTGTSGPQHH560 pKa = 5.54GTVVVNTDD568 pKa = 2.8GTFTYY573 pKa = 9.57TPSGNYY579 pKa = 10.03SGTDD583 pKa = 3.1TFKK586 pKa = 9.75ITVSDD591 pKa = 3.67GHH593 pKa = 6.66GGTATEE599 pKa = 4.4TVNVTVEE606 pKa = 4.04NGAPTLTTANAATANDD622 pKa = 3.56HH623 pKa = 6.24SAVTGHH629 pKa = 5.31VTGSDD634 pKa = 3.55TPGDD638 pKa = 3.75TLSYY642 pKa = 11.13SLVDD646 pKa = 3.4ANGNHH651 pKa = 5.94VGSLVTDD658 pKa = 4.06HH659 pKa = 6.28GTVSINAATGDD670 pKa = 3.73YY671 pKa = 10.47TFTPNAANASLGVGASVTDD690 pKa = 3.56SFKK693 pKa = 11.3VVATDD698 pKa = 3.38NHH700 pKa = 6.33GADD703 pKa = 3.76SNLGTVAVTVTGSNDD718 pKa = 3.22GPVVTGVTGGTGNEE732 pKa = 4.66SITSARR738 pKa = 11.84SVVTGHH744 pKa = 6.88ISAGDD749 pKa = 3.48VDD751 pKa = 5.39AGDD754 pKa = 3.74TLSYY758 pKa = 11.11SVTDD762 pKa = 3.55NAAAGHH768 pKa = 6.56HH769 pKa = 5.97GTLAVNASGDD779 pKa = 3.82YY780 pKa = 10.58TFTASDD786 pKa = 4.32SNWHH790 pKa = 5.83GTDD793 pKa = 2.85SFTVQVSDD801 pKa = 3.5GHH803 pKa = 6.4GGTVNQTVAITVNAQADD820 pKa = 3.66AATINAQDD828 pKa = 3.77ASMAAQSGATFTASGGYY845 pKa = 10.17AEE847 pKa = 5.39GGSGSDD853 pKa = 3.88IINGSSANDD862 pKa = 3.38ILYY865 pKa = 10.8GDD867 pKa = 4.96DD868 pKa = 3.71PSGDD872 pKa = 3.76TEE874 pKa = 4.31VTVALNISAAAISGEE889 pKa = 4.35SVSSITLSNLPGGAVLNHH907 pKa = 5.9GHH909 pKa = 7.41DD910 pKa = 3.93NGDD913 pKa = 3.89GTWTLAPGDD922 pKa = 4.08LTGLTLTSNNGIGGTVDD939 pKa = 3.35VAVTTLDD946 pKa = 3.62GTSTAVTNSSFNVSFSGGFNDD967 pKa = 5.19TITGGVGADD976 pKa = 3.33TMYY979 pKa = 11.19GGAGNDD985 pKa = 3.43TFKK988 pKa = 11.29VSGASEE994 pKa = 4.43STGDD998 pKa = 3.69IYY1000 pKa = 11.39DD1001 pKa = 4.27GGSGTDD1007 pKa = 3.29TVLGSSGNDD1016 pKa = 3.33VISVTSNLGNMRR1028 pKa = 11.84SIEE1031 pKa = 4.27VIDD1034 pKa = 4.43GGAGTDD1040 pKa = 3.43TLLAGSGNDD1049 pKa = 3.36YY1050 pKa = 11.23LDD1052 pKa = 3.97FSGTTIRR1059 pKa = 11.84GVEE1062 pKa = 4.3VIDD1065 pKa = 3.54TGAGNDD1071 pKa = 4.06VITGSAGADD1080 pKa = 3.49TILTGTGDD1088 pKa = 3.81DD1089 pKa = 3.5TVVAIGGQTAGDD1101 pKa = 4.14VYY1103 pKa = 11.32DD1104 pKa = 5.0GGAGTDD1110 pKa = 3.59TLNVDD1115 pKa = 4.67LTPAQYY1121 pKa = 7.47TTAVRR1126 pKa = 11.84LEE1128 pKa = 4.15LMSFASFVDD1137 pKa = 3.85NPANAGKK1144 pKa = 10.47SFTFQSLGGLKK1155 pKa = 8.96ATNFEE1160 pKa = 4.41DD1161 pKa = 4.74LRR1163 pKa = 11.84VTVDD1167 pKa = 3.86GNDD1170 pKa = 2.86VDD1172 pKa = 5.78LNHH1175 pKa = 7.3PPQVTSVEE1183 pKa = 4.38SNYY1186 pKa = 9.72TAGHH1190 pKa = 6.63ADD1192 pKa = 3.71GVVHH1196 pKa = 6.57ATDD1199 pKa = 3.59SDD1201 pKa = 4.36GDD1203 pKa = 3.85TMSYY1207 pKa = 11.14SFGTNTNGTPITSMATAHH1225 pKa = 5.75GTVTIDD1231 pKa = 3.04SSTGEE1236 pKa = 4.04YY1237 pKa = 10.52HH1238 pKa = 6.04FTASDD1243 pKa = 3.71ANFKK1247 pKa = 9.65GTDD1250 pKa = 3.38TFSVTVKK1257 pKa = 10.67DD1258 pKa = 3.96GMGGTTTQKK1267 pKa = 10.72VNLDD1271 pKa = 3.83FNPGDD1276 pKa = 3.95DD1277 pKa = 3.69ATVVTGPTSLGGSDD1291 pKa = 4.99EE1292 pKa = 4.16DD1293 pKa = 3.78TQTFIRR1299 pKa = 11.84TDD1301 pKa = 3.29QLLANATDD1309 pKa = 3.57VDD1311 pKa = 4.21NTLHH1315 pKa = 5.81VANLSAVDD1323 pKa = 3.23SHH1325 pKa = 6.37GAVVGSFVHH1334 pKa = 6.91AVDD1337 pKa = 4.58ADD1339 pKa = 3.76GHH1341 pKa = 6.43DD1342 pKa = 4.31GYY1344 pKa = 11.68AFTPNANFAGDD1355 pKa = 3.39VTVNYY1360 pKa = 10.64DD1361 pKa = 3.14VVTDD1365 pKa = 3.58TGIATHH1371 pKa = 6.57TSGSLHH1377 pKa = 6.24VDD1379 pKa = 3.75AVADD1383 pKa = 3.66AATFDD1388 pKa = 3.74ATIGSNGVSVAHH1400 pKa = 7.05LGNDD1404 pKa = 3.35SKK1406 pKa = 11.66VVNLFVNDD1414 pKa = 4.05TSSGTAGFDD1423 pKa = 2.91IYY1425 pKa = 11.2LGGTNIGHH1433 pKa = 4.93YY1434 pKa = 9.09TVGRR1438 pKa = 11.84SYY1440 pKa = 11.51SSDD1443 pKa = 2.9KK1444 pKa = 11.1SVSLTLTDD1452 pKa = 3.76AQHH1455 pKa = 5.56TQLLNGADD1463 pKa = 3.42IKK1465 pKa = 11.05IVDD1468 pKa = 3.59NDD1470 pKa = 4.1GYY1472 pKa = 8.98NTRR1475 pKa = 11.84DD1476 pKa = 3.45VLVDD1480 pKa = 4.6RR1481 pKa = 11.84IQVDD1485 pKa = 3.55GLTFQAEE1492 pKa = 4.08NGILSGASTTSGYY1505 pKa = 11.01YY1506 pKa = 9.87GVSSEE1511 pKa = 4.43TYY1513 pKa = 10.14ARR1515 pKa = 11.84LNNVGSSVTFDD1526 pKa = 3.53FDD1528 pKa = 4.2SSVSHH1533 pKa = 6.56TSSGYY1538 pKa = 9.58HH1539 pKa = 6.06VDD1541 pKa = 4.55LAGHH1545 pKa = 7.09LNDD1548 pKa = 4.02TDD1550 pKa = 3.89GSEE1553 pKa = 4.15TLSYY1557 pKa = 10.54HH1558 pKa = 6.21IDD1560 pKa = 3.34AMTTGASLSYY1570 pKa = 9.79TGNEE1574 pKa = 3.68GTLVHH1579 pKa = 6.05NTDD1582 pKa = 3.66GSWDD1586 pKa = 3.83FNVDD1590 pKa = 2.78ASHH1593 pKa = 6.92YY1594 pKa = 10.62DD1595 pKa = 3.36GAVSLNLNVANGTAGFDD1612 pKa = 3.38VHH1614 pKa = 5.44VTANATEE1621 pKa = 4.09TSNLDD1626 pKa = 3.27VAGVGDD1632 pKa = 4.08TVHH1635 pKa = 7.28CDD1637 pKa = 3.27GAIGGAGAIPGITLVGNAGNDD1658 pKa = 4.06DD1659 pKa = 4.0IIGTNGNDD1667 pKa = 3.53LLLGGKK1673 pKa = 9.8GGATYY1678 pKa = 10.35RR1679 pKa = 11.84FSGGGCGSGGGWSIVQSDD1697 pKa = 4.1TNDD1700 pKa = 3.46VISAGAGDD1708 pKa = 3.87DD1709 pKa = 4.15VIYY1712 pKa = 11.07GDD1714 pKa = 3.94ARR1716 pKa = 11.84LVNGNIQITGSGNDD1730 pKa = 3.48VLDD1733 pKa = 4.34GGSGNDD1739 pKa = 3.86QIHH1742 pKa = 6.5GGAGNDD1748 pKa = 3.89TIIGGTGDD1756 pKa = 4.03DD1757 pKa = 5.02VMFGDD1762 pKa = 4.34QGNDD1766 pKa = 2.65TFLFDD1771 pKa = 4.72FGFGHH1776 pKa = 7.17DD1777 pKa = 3.76VVDD1780 pKa = 4.93GGRR1783 pKa = 11.84GSNWTDD1789 pKa = 3.21TLDD1792 pKa = 3.65LTHH1795 pKa = 7.57DD1796 pKa = 3.87NQISSVNIEE1805 pKa = 4.54GVSGWAVSVDD1815 pKa = 3.54AQGHH1819 pKa = 5.35HH1820 pKa = 6.2VAQATNGAHH1829 pKa = 7.07DD1830 pKa = 4.45ANGTIVVTNHH1840 pKa = 7.04DD1841 pKa = 4.03GSQDD1845 pKa = 3.32TIEE1848 pKa = 3.94FHH1850 pKa = 6.8NVEE1853 pKa = 4.2KK1854 pKa = 11.09VVWW1857 pKa = 3.61

Molecular weight:
185.61 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q2W777|Q2W777_MAGSA Retron-type reverse transcriptase OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=amb1494 PE=4 SV=1
MM1 pKa = 7.53IGALLRR7 pKa = 11.84RR8 pKa = 11.84LRR10 pKa = 11.84RR11 pKa = 11.84FFRR14 pKa = 11.84PPGSPVVIQSVRR26 pKa = 11.84GLSAPRR32 pKa = 11.84HH33 pKa = 4.87PQNN36 pKa = 3.88

Molecular weight:
4.08 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4515

0

4515

1451216

34

9529

321.4

34.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.644 ± 0.056

0.992 ± 0.016

5.671 ± 0.037

5.71 ± 0.05

3.392 ± 0.022

8.737 ± 0.054

2.135 ± 0.019

4.706 ± 0.027

3.51 ± 0.033

10.26 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.668 ± 0.02

2.489 ± 0.034

5.218 ± 0.049

3.093 ± 0.024

7.034 ± 0.07

5.468 ± 0.046

5.307 ± 0.089

7.613 ± 0.031

1.363 ± 0.016

1.99 ± 0.021

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski