Rosellinia necatrix mycoreovirus 3 (isolate W370) (RnMYRV-3) (Rosellinia anti-rot virus)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Mycoreovirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8JXF4|Q8JXF4_MYRVW p6 OS=Rosellinia necatrix mycoreovirus 3 (isolate W370) OX=311229 PE=4 SV=1
MM1 pKa = 7.29AQQVHH6 pKa = 6.28AVQLSPRR13 pKa = 11.84LRR15 pKa = 11.84NDD17 pKa = 2.91VLLQLTKK24 pKa = 10.5RR25 pKa = 11.84SLMTIDD31 pKa = 3.73TGTILQQVEE40 pKa = 4.29LPLNLFLLGVCFVGDD55 pKa = 3.45GDD57 pKa = 4.43VPYY60 pKa = 11.28LLIRR64 pKa = 11.84QIAGYY69 pKa = 7.09TWHH72 pKa = 7.62IDD74 pKa = 3.14PGYY77 pKa = 9.45GIEE80 pKa = 4.13MYY82 pKa = 10.21EE83 pKa = 3.91VDD85 pKa = 4.33VGEE88 pKa = 4.46EE89 pKa = 4.13MPVLQARR96 pKa = 11.84IDD98 pKa = 3.63SLAYY102 pKa = 10.85VMTIDD107 pKa = 4.29EE108 pKa = 4.72RR109 pKa = 11.84DD110 pKa = 3.38YY111 pKa = 10.54LYY113 pKa = 10.35PVITVDD119 pKa = 3.84EE120 pKa = 4.91DD121 pKa = 3.66GRR123 pKa = 11.84TLNINYY129 pKa = 9.12TDD131 pKa = 4.31DD132 pKa = 3.91PSLRR136 pKa = 11.84HH137 pKa = 6.79DD138 pKa = 3.44VDD140 pKa = 3.61TYY142 pKa = 11.73ARR144 pKa = 11.84LSRR147 pKa = 11.84EE148 pKa = 3.99LQLVRR153 pKa = 11.84DD154 pKa = 4.86DD155 pKa = 3.65ISVRR159 pKa = 11.84SHH161 pKa = 6.77DD162 pKa = 4.49RR163 pKa = 11.84RR164 pKa = 11.84PDD166 pKa = 3.37SPSIYY171 pKa = 9.26SDD173 pKa = 2.97QRR175 pKa = 11.84SRR177 pKa = 11.84PNTPPRR183 pKa = 11.84PITPPYY189 pKa = 9.94LRR191 pKa = 11.84DD192 pKa = 3.45YY193 pKa = 11.03DD194 pKa = 4.15VEE196 pKa = 4.77SEE198 pKa = 4.96DD199 pKa = 3.6DD200 pKa = 3.82TEE202 pKa = 5.83DD203 pKa = 3.53GFVRR207 pKa = 11.84ISYY210 pKa = 10.56ASLRR214 pKa = 11.84AAYY217 pKa = 9.24RR218 pKa = 11.84PPNTTTTLVRR228 pKa = 11.84SMLYY232 pKa = 10.42NPIPVPPPQPLEE244 pKa = 4.31TRR246 pKa = 11.84WSTFYY251 pKa = 11.62AMDD254 pKa = 4.62ADD256 pKa = 3.91LRR258 pKa = 11.84HH259 pKa = 6.74KK260 pKa = 10.23IRR262 pKa = 11.84HH263 pKa = 5.37SLNFPLSHH271 pKa = 6.53GEE273 pKa = 3.56WRR275 pKa = 11.84LSDD278 pKa = 3.33NALRR282 pKa = 11.84GVGMMPRR289 pKa = 11.84PSGGVYY295 pKa = 7.14QHH297 pKa = 6.93ISHH300 pKa = 6.8MCNDD304 pKa = 3.71PEE306 pKa = 4.7SRR308 pKa = 11.84LLQSTEE314 pKa = 4.05WEE316 pKa = 4.05HH317 pKa = 6.14MIYY320 pKa = 9.94MIDD323 pKa = 3.78KK324 pKa = 10.41RR325 pKa = 11.84YY326 pKa = 9.62IDD328 pKa = 4.23LGAVCSNGLGATVLSIDD345 pKa = 3.63AATSLEE351 pKa = 3.98MAKK354 pKa = 10.52DD355 pKa = 3.44VLYY358 pKa = 11.03LFGRR362 pKa = 11.84AVVTFRR368 pKa = 11.84EE369 pKa = 4.82WKK371 pKa = 10.47RR372 pKa = 11.84EE373 pKa = 3.86TADD376 pKa = 3.52EE377 pKa = 4.41NEE379 pKa = 3.82LLMRR383 pKa = 11.84VFPDD387 pKa = 3.83KK388 pKa = 10.75IITVVDD394 pKa = 3.31YY395 pKa = 11.29SYY397 pKa = 11.92LNTFKK402 pKa = 11.03YY403 pKa = 10.51RR404 pKa = 11.84IDD406 pKa = 3.21QRR408 pKa = 11.84EE409 pKa = 4.05NVRR412 pKa = 11.84MVLGLINGALSDD424 pKa = 4.02SNRR427 pKa = 11.84EE428 pKa = 3.35EE429 pKa = 4.2WASVLALIYY438 pKa = 10.0MVTNRR443 pKa = 11.84QLPGSQDD450 pKa = 3.1AGVDD454 pKa = 4.01DD455 pKa = 3.56IHH457 pKa = 8.47VLLRR461 pKa = 11.84PPEE464 pKa = 4.65SGLSYY469 pKa = 10.98LVLLALIIVVSDD481 pKa = 3.44RR482 pKa = 3.67

Molecular weight: 55.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8JXF5|Q8JXF5_MYRVW PX OS=Rosellinia necatrix mycoreovirus 3 (isolate W370) OX=311229 PE=4 SV=1
MM1 pKa = 7.13QHH3 pKa = 5.85MSHH6 pKa = 5.24YY7 pKa = 9.9HH8 pKa = 5.41FRR10 pKa = 11.84LICTGPYY17 pKa = 9.66AVLRR21 pKa = 11.84IILNDD26 pKa = 3.42CTPPMLHH33 pKa = 5.94GTLMSSQTTGKK44 pKa = 8.74PQYY47 pKa = 10.36LRR49 pKa = 11.84AVPHH53 pKa = 6.6IFMIDD58 pKa = 3.34SMDD61 pKa = 3.55KK62 pKa = 10.78FSTSHH67 pKa = 5.96ATLLSKK73 pKa = 10.8CPQEE77 pKa = 4.27YY78 pKa = 9.89VSSDD82 pKa = 2.66ITVITSPTAQDD93 pKa = 2.93IARR96 pKa = 11.84SIRR99 pKa = 11.84ANKK102 pKa = 9.97HH103 pKa = 3.99VVCATLPVGVFNDD116 pKa = 4.7AIVFSSITVPDD127 pKa = 3.4CTKK130 pKa = 9.05YY131 pKa = 10.77LRR133 pKa = 11.84RR134 pKa = 11.84TFHH137 pKa = 6.63ALLRR141 pKa = 11.84RR142 pKa = 11.84LSFGHH147 pKa = 5.88SCYY150 pKa = 10.57SCSNVLQLSLPPWLSVLASGWKK172 pKa = 9.74RR173 pKa = 11.84YY174 pKa = 9.44VPSSSNDD181 pKa = 3.45DD182 pKa = 3.4LAKK185 pKa = 10.43FYY187 pKa = 10.02LQPSSSPNQIVTNVQDD203 pKa = 3.81LQDD206 pKa = 3.69FVTNQLKK213 pKa = 10.57SDD215 pKa = 3.53VDD217 pKa = 3.66VVYY220 pKa = 10.68FDD222 pKa = 6.22DD223 pKa = 4.77VLTTDD228 pKa = 5.41GITIGEE234 pKa = 4.54KK235 pKa = 9.85IRR237 pKa = 11.84STGAQFNVARR247 pKa = 11.84DD248 pKa = 3.66LTHH251 pKa = 7.06FNIIKK256 pKa = 9.53QRR258 pKa = 11.84YY259 pKa = 7.6VEE261 pKa = 4.02QTSIRR266 pKa = 11.84TRR268 pKa = 11.84RR269 pKa = 11.84QHH271 pKa = 6.93KK272 pKa = 10.13KK273 pKa = 10.03DD274 pKa = 3.16LHH276 pKa = 5.43ASKK279 pKa = 10.6RR280 pKa = 11.84KK281 pKa = 6.23QTITARR287 pKa = 11.84VFPPVYY293 pKa = 10.39LFDD296 pKa = 3.19ITVTIKK302 pKa = 9.95SWSSAMPMSQQRR314 pKa = 11.84RR315 pKa = 11.84LSRR318 pKa = 11.84VYY320 pKa = 10.91SDD322 pKa = 5.91LIQLSKK328 pKa = 11.15KK329 pKa = 9.65PLTVLHH335 pKa = 5.94MLSWDD340 pKa = 3.83GPNTISTDD348 pKa = 3.57DD349 pKa = 5.2DD350 pKa = 3.08IWSQFTEE357 pKa = 3.93WLFGVVQNVDD367 pKa = 2.81EE368 pKa = 4.44TMARR372 pKa = 11.84LLTAIPYY379 pKa = 7.7MSVMVAVSRR388 pKa = 11.84RR389 pKa = 11.84GFGTWIDD396 pKa = 3.69FRR398 pKa = 11.84NWTEE402 pKa = 4.07GFSLSKK408 pKa = 10.41ILVGRR413 pKa = 11.84TYY415 pKa = 10.47MVGKK419 pKa = 9.93KK420 pKa = 10.13NSGKK424 pKa = 10.94GMIGKK429 pKa = 8.59LIWKK433 pKa = 9.44LGVPVIDD440 pKa = 3.52SDD442 pKa = 4.16DD443 pKa = 3.68YY444 pKa = 12.01GRR446 pKa = 11.84VLLIAEE452 pKa = 4.36DD453 pKa = 3.35QGVPLNEE460 pKa = 3.57AVHH463 pKa = 5.98RR464 pKa = 11.84HH465 pKa = 5.36FSLTYY470 pKa = 7.42QQRR473 pKa = 11.84DD474 pKa = 3.28TAPTVFEE481 pKa = 4.58KK482 pKa = 11.1AMDD485 pKa = 5.14DD486 pKa = 3.39IVRR489 pKa = 11.84DD490 pKa = 3.73LAVVRR495 pKa = 11.84YY496 pKa = 9.03AIPEE500 pKa = 3.9VDD502 pKa = 4.14HH503 pKa = 6.97PALIAFGRR511 pKa = 11.84VYY513 pKa = 11.21DD514 pKa = 4.32EE515 pKa = 4.27LTSKK519 pKa = 10.83YY520 pKa = 10.3KK521 pKa = 10.33YY522 pKa = 10.47ADD524 pKa = 3.27FEE526 pKa = 4.53RR527 pKa = 11.84VVRR530 pKa = 11.84SHH532 pKa = 6.64IASEE536 pKa = 4.63GVLAPDD542 pKa = 3.56GTLLKK547 pKa = 10.57VDD549 pKa = 3.85DD550 pKa = 4.35RR551 pKa = 11.84FVFSTHH557 pKa = 6.2CSEE560 pKa = 5.02EE561 pKa = 3.95ATQVLGANYY570 pKa = 8.8MFQLSTAIDD579 pKa = 3.77SYY581 pKa = 11.36IGVLLRR587 pKa = 11.84GQHH590 pKa = 5.87SNAISEE596 pKa = 4.48LMLAVYY602 pKa = 10.0YY603 pKa = 10.69DD604 pKa = 4.57RR605 pKa = 11.84IHH607 pKa = 7.83VNIFDD612 pKa = 4.57LVPTGVVCTVLRR624 pKa = 11.84TGLARR629 pKa = 11.84LVPKK633 pKa = 10.64AA634 pKa = 3.7

Molecular weight: 71.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

7721

265

1360

643.4

71.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.696 ± 0.282

1.567 ± 0.116

6.282 ± 0.268

4.352 ± 0.256

4.287 ± 0.256

6.178 ± 0.34

2.953 ± 0.219

5.453 ± 0.186

2.862 ± 0.281

8.937 ± 0.312

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.875 ± 0.14

3.536 ± 0.267

5.44 ± 0.226

3.393 ± 0.205

6.774 ± 0.389

8.432 ± 0.504

6.567 ± 0.244

8.483 ± 0.376

1.295 ± 0.113

3.639 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski