Methanohalophilus mahii (strain ATCC 35705 / DSM 5219 / SLP)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; Methanosarcinaceae; Methanohalophilus; Methanohalophilus mahii

Average proteome isoelectric point is 5.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1986 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5E9L3|D5E9L3_METMS ORC1-type DNA replication protein OS=Methanohalophilus mahii (strain ATCC 35705 / DSM 5219 / SLP) OX=547558 GN=Mmah_0332 PE=3 SV=1
MM1 pKa = 7.08KK2 pKa = 9.93TGFKK6 pKa = 10.05ILLIGFMIIGMGCGVAAAEE25 pKa = 4.31SAPNASVTNVSSDD38 pKa = 3.27VDD40 pKa = 3.71SATIDD45 pKa = 3.72FNVNQSDD52 pKa = 3.31ASTRR56 pKa = 11.84IAYY59 pKa = 8.5GISNSLSSWSDD70 pKa = 2.78WNYY73 pKa = 10.09EE74 pKa = 3.98DD75 pKa = 4.29SNSRR79 pKa = 11.84SIVLSEE85 pKa = 4.45LNDD88 pKa = 3.24GTMYY92 pKa = 10.58YY93 pKa = 10.49YY94 pKa = 10.62SVYY97 pKa = 10.56AYY99 pKa = 10.81NGTNDD104 pKa = 3.25SFVYY108 pKa = 9.15NTTISTFNTDD118 pKa = 4.09SPTAPNASVTNVSSDD133 pKa = 3.27VDD135 pKa = 3.71SATIDD140 pKa = 3.72FNVNQSDD147 pKa = 3.31ASTRR151 pKa = 11.84IAYY154 pKa = 8.5GISNSLSSWSDD165 pKa = 2.78WNYY168 pKa = 10.09EE169 pKa = 3.98DD170 pKa = 4.29SNSRR174 pKa = 11.84SIVLSEE180 pKa = 4.0LNDD183 pKa = 3.19RR184 pKa = 11.84TMYY187 pKa = 10.27YY188 pKa = 10.33YY189 pKa = 10.61SVYY192 pKa = 10.56AYY194 pKa = 10.81NGTNDD199 pKa = 3.25SFVYY203 pKa = 9.04NTTIATLNTTSPTAPNASVTNVSSDD228 pKa = 3.27VDD230 pKa = 3.71SATIDD235 pKa = 3.72FNVNQSDD242 pKa = 3.31ASTRR246 pKa = 11.84IAYY249 pKa = 8.5GISNSLSSWSDD260 pKa = 2.78WNYY263 pKa = 10.09EE264 pKa = 3.98DD265 pKa = 4.29SNSRR269 pKa = 11.84SIVLSEE275 pKa = 4.45LNDD278 pKa = 3.24GTMYY282 pKa = 10.58YY283 pKa = 10.49YY284 pKa = 10.62SVYY287 pKa = 10.56AYY289 pKa = 10.81NGTNDD294 pKa = 3.25SFVYY298 pKa = 9.04NTTIATLNTTSPTAPSISDD317 pKa = 3.05ISSSNIGGDD326 pKa = 2.92RR327 pKa = 11.84ATINYY332 pKa = 8.29VVNQSDD338 pKa = 3.94APTRR342 pKa = 11.84IAYY345 pKa = 7.37GTSSDD350 pKa = 3.43LSSYY354 pKa = 9.51EE355 pKa = 3.44WSGWFNDD362 pKa = 3.69STVHH366 pKa = 6.92NEE368 pKa = 4.14TISNLNGSTKK378 pKa = 10.37YY379 pKa = 10.5YY380 pKa = 9.87YY381 pKa = 10.44SIYY384 pKa = 10.53AYY386 pKa = 10.47NEE388 pKa = 4.13SNNSVFYY395 pKa = 10.69NSSIEE400 pKa = 4.15SFTTISNWGDD410 pKa = 3.65RR411 pKa = 11.84IWDD414 pKa = 3.61EE415 pKa = 5.43KK416 pKa = 11.41ANQSNKK422 pKa = 7.55YY423 pKa = 7.11TWDD426 pKa = 3.22GRR428 pKa = 11.84SFSGFYY434 pKa = 10.55YY435 pKa = 10.81DD436 pKa = 6.14LDD438 pKa = 3.83TGDD441 pKa = 3.92TSEE444 pKa = 5.18SMTITIDD451 pKa = 3.43GRR453 pKa = 11.84RR454 pKa = 11.84IDD456 pKa = 5.57DD457 pKa = 3.79GDD459 pKa = 3.76LVYY462 pKa = 8.25TTKK465 pKa = 10.78PIDD468 pKa = 3.23KK469 pKa = 9.47EE470 pKa = 4.38FEE472 pKa = 3.97FGSWGEE478 pKa = 3.88YY479 pKa = 9.92QIIGFMAEE487 pKa = 4.29RR488 pKa = 11.84YY489 pKa = 8.18FAGYY493 pKa = 9.7SVNTSSDD500 pKa = 3.29VTGDD504 pKa = 3.82DD505 pKa = 3.42VSLISDD511 pKa = 4.18GVLSKK516 pKa = 11.13VLIDD520 pKa = 3.71TDD522 pKa = 4.16DD523 pKa = 4.59DD524 pKa = 4.27EE525 pKa = 5.33SVYY528 pKa = 10.96SGSSLTLEE536 pKa = 4.33EE537 pKa = 5.43GYY539 pKa = 10.69SLNIKK544 pKa = 9.82QVDD547 pKa = 3.8VNGDD551 pKa = 3.98SVWISLTKK559 pKa = 10.91DD560 pKa = 2.87GDD562 pKa = 4.09EE563 pKa = 4.24VDD565 pKa = 4.85DD566 pKa = 6.1AILSSDD572 pKa = 3.15EE573 pKa = 4.26TYY575 pKa = 11.0VYY577 pKa = 10.6EE578 pKa = 4.98KK579 pKa = 11.06DD580 pKa = 3.24LGGVDD585 pKa = 4.56DD586 pKa = 4.52VPVIAVHH593 pKa = 6.54IDD595 pKa = 3.39DD596 pKa = 4.51VFSGTEE602 pKa = 3.85TNAVFIDD609 pKa = 4.89GIFQISDD616 pKa = 3.51DD617 pKa = 4.03YY618 pKa = 12.04VEE620 pKa = 4.1VDD622 pKa = 3.23RR623 pKa = 11.84GDD625 pKa = 3.41EE626 pKa = 4.12FGEE629 pKa = 4.18MEE631 pKa = 4.4VTSLSSTEE639 pKa = 3.32IKK641 pKa = 9.92MEE643 pKa = 4.23NEE645 pKa = 3.93DD646 pKa = 5.33SISLDD651 pKa = 3.25EE652 pKa = 4.95GDD654 pKa = 5.25IIDD657 pKa = 4.97IMGKK661 pKa = 10.13LKK663 pKa = 10.86FIVADD668 pKa = 4.23DD669 pKa = 3.88NDD671 pKa = 3.93VLRR674 pKa = 11.84FAPFVDD680 pKa = 3.76MSEE683 pKa = 4.08PGTYY687 pKa = 9.25EE688 pKa = 3.51LRR690 pKa = 11.84GTIAEE695 pKa = 4.49DD696 pKa = 4.04EE697 pKa = 4.59GFSWTPLNFEE707 pKa = 4.04GFYY710 pKa = 10.87YY711 pKa = 10.65DD712 pKa = 3.07IDD714 pKa = 3.59EE715 pKa = 4.57GLRR718 pKa = 11.84SEE720 pKa = 4.52SLNVTNYY727 pKa = 10.26NGRR730 pKa = 11.84NIDD733 pKa = 4.04AGDD736 pKa = 5.11LIYY739 pKa = 10.85KK740 pKa = 6.86STPVEE745 pKa = 4.07VEE747 pKa = 3.86FEE749 pKa = 3.96YY750 pKa = 11.4GPWGKK755 pKa = 10.9YY756 pKa = 7.71NVIGFMAEE764 pKa = 3.95KK765 pKa = 10.57YY766 pKa = 9.38FAGYY770 pKa = 10.31SGDD773 pKa = 3.71TSDD776 pKa = 6.41DD777 pKa = 3.37ITDD780 pKa = 3.69DD781 pKa = 4.29TISLMSSGQLSKK793 pKa = 11.38VLIDD797 pKa = 5.23DD798 pKa = 4.84DD799 pKa = 4.88DD800 pKa = 4.28EE801 pKa = 6.5KK802 pKa = 11.42SVYY805 pKa = 9.45TGSSLILEE813 pKa = 4.41EE814 pKa = 5.82GYY816 pKa = 11.12ALNIRR821 pKa = 11.84EE822 pKa = 4.05IDD824 pKa = 3.77VNGDD828 pKa = 2.85RR829 pKa = 11.84VMIQLTQDD837 pKa = 2.9GDD839 pKa = 4.14NIEE842 pKa = 4.25SAIVSSDD849 pKa = 3.38DD850 pKa = 3.49EE851 pKa = 4.48YY852 pKa = 11.79VYY854 pKa = 11.05EE855 pKa = 5.2KK856 pKa = 11.06DD857 pKa = 4.56VGDD860 pKa = 4.28EE861 pKa = 4.1DD862 pKa = 4.38DD863 pKa = 3.8VPIIVVHH870 pKa = 6.02FNEE873 pKa = 4.5IFSGTEE879 pKa = 3.46TSAVFVEE886 pKa = 5.59GIFQISEE893 pKa = 4.24DD894 pKa = 3.9YY895 pKa = 11.56LEE897 pKa = 5.41VEE899 pKa = 5.12DD900 pKa = 5.75GDD902 pKa = 4.44DD903 pKa = 3.9FGVMTVDD910 pKa = 3.44STSGSIILEE919 pKa = 4.18NEE921 pKa = 3.87DD922 pKa = 5.49NIDD925 pKa = 3.88LDD927 pKa = 4.05EE928 pKa = 5.98GDD930 pKa = 4.7TIDD933 pKa = 4.79IMGEE937 pKa = 3.87IKK939 pKa = 10.58FKK941 pKa = 11.13VADD944 pKa = 4.12DD945 pKa = 4.05SSTVRR950 pKa = 11.84YY951 pKa = 9.26YY952 pKa = 10.49PFVEE956 pKa = 4.66VEE958 pKa = 4.22TKK960 pKa = 10.34PYY962 pKa = 10.76DD963 pKa = 3.56SLDD966 pKa = 3.18IDD968 pKa = 3.81IEE970 pKa = 4.18PTTVVEE976 pKa = 4.07GDD978 pKa = 3.91EE979 pKa = 4.04ITFTVTSRR987 pKa = 11.84GAAIRR992 pKa = 11.84DD993 pKa = 3.54ASILIHH999 pKa = 6.48GDD1001 pKa = 2.98RR1002 pKa = 11.84VGITSDD1008 pKa = 3.29EE1009 pKa = 4.38GVFEE1013 pKa = 4.35YY1014 pKa = 11.0DD1015 pKa = 3.98ADD1017 pKa = 4.09DD1018 pKa = 4.18AGKK1021 pKa = 8.46FTATAEE1027 pKa = 4.15KK1028 pKa = 10.09EE1029 pKa = 4.58GYY1031 pKa = 9.9VSSKK1035 pKa = 11.31GNFEE1039 pKa = 4.6VISPDD1044 pKa = 3.9DD1045 pKa = 3.84EE1046 pKa = 4.58SKK1048 pKa = 10.87KK1049 pKa = 10.54ISIEE1053 pKa = 3.89VSPEE1057 pKa = 3.59EE1058 pKa = 4.26VFEE1061 pKa = 4.2GTPITISVVKK1071 pKa = 10.62AIGSEE1076 pKa = 3.94PLEE1079 pKa = 4.24GAEE1082 pKa = 4.27VFFDD1086 pKa = 3.85GKK1088 pKa = 11.12SLGEE1092 pKa = 3.95TDD1094 pKa = 3.54EE1095 pKa = 5.79DD1096 pKa = 4.05GTITYY1101 pKa = 9.83NPKK1104 pKa = 10.46EE1105 pKa = 4.33PGTHH1109 pKa = 6.5KK1110 pKa = 10.51IEE1112 pKa = 4.41AKK1114 pKa = 10.04VDD1116 pKa = 3.76GYY1118 pKa = 11.64LDD1120 pKa = 3.74AEE1122 pKa = 4.56LNIKK1126 pKa = 10.1VNEE1129 pKa = 3.93LAANFEE1135 pKa = 4.29FSNLRR1140 pKa = 11.84IDD1142 pKa = 4.5PLPATAGEE1150 pKa = 4.58EE1151 pKa = 4.18FTVMLDD1157 pKa = 4.54SINTGNAEE1165 pKa = 3.57GSYY1168 pKa = 9.28TVEE1171 pKa = 4.82LSINDD1176 pKa = 3.64NVVDD1180 pKa = 3.94SQEE1183 pKa = 3.76ITLGQNNSTTIEE1195 pKa = 4.15FTSTAGEE1202 pKa = 4.17PGTYY1206 pKa = 9.43LVKK1209 pKa = 10.9AGGLSTTMDD1218 pKa = 3.56VEE1220 pKa = 4.55EE1221 pKa = 4.63GTSIVWYY1228 pKa = 10.21VLGGLVLVLGGGAAYY1243 pKa = 9.66IFATGGTAKK1252 pKa = 10.02FAEE1255 pKa = 4.46IAEE1258 pKa = 4.51SLRR1261 pKa = 11.84SRR1263 pKa = 11.84LGRR1266 pKa = 3.68

Molecular weight: 138.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5EC60|D5EC60_METMS Uncharacterized protein OS=Methanohalophilus mahii (strain ATCC 35705 / DSM 5219 / SLP) OX=547558 GN=Mmah_1257 PE=4 SV=1
MM1 pKa = 7.52DD2 pKa = 3.41QVRR5 pKa = 11.84QVSHH9 pKa = 4.82NTKK12 pKa = 8.26GQKK15 pKa = 8.66MRR17 pKa = 11.84LAKK20 pKa = 10.61AHH22 pKa = 5.45VQNQRR27 pKa = 11.84VPTWAIIKK35 pKa = 9.51SNRR38 pKa = 11.84KK39 pKa = 8.84VVSHH43 pKa = 6.56PKK45 pKa = 8.36RR46 pKa = 11.84RR47 pKa = 11.84HH48 pKa = 3.55WRR50 pKa = 11.84RR51 pKa = 11.84NSLKK55 pKa = 10.72VKK57 pKa = 10.47

Molecular weight: 6.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1986

0

1986

589724

31

3593

296.9

33.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.122 ± 0.061

1.304 ± 0.032

6.221 ± 0.053

7.659 ± 0.06

3.784 ± 0.041

7.384 ± 0.053

1.942 ± 0.024

8.012 ± 0.048

6.112 ± 0.061

8.762 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.927 ± 0.03

4.462 ± 0.066

3.888 ± 0.029

2.757 ± 0.028

4.435 ± 0.056

6.457 ± 0.057

5.306 ± 0.053

7.196 ± 0.047

0.845 ± 0.018

3.423 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski