Paracoccidioides lutzii (strain ATCC MYA-826 / Pb01) (Paracoccidioides brasiliensis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales; Onygenales incertae sedis; Paracoccidioides; Paracoccidioides lutzii

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8811 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A2V3E6|A0A0A2V3E6_PARBA Uncharacterized protein (Fragment) OS=Paracoccidioides lutzii (strain ATCC MYA-826 / Pb01) OX=502779 GN=PAAG_12703 PE=4 SV=1
MM1 pKa = 7.51AFYY4 pKa = 10.58YY5 pKa = 10.56LKK7 pKa = 10.45LVHH10 pKa = 6.82FSTYY14 pKa = 9.64EE15 pKa = 3.65EE16 pKa = 4.65SNIKK20 pKa = 10.65HH21 pKa = 5.92NFHH24 pKa = 6.99NILIHH29 pKa = 4.81VHH31 pKa = 5.7FTPTVNLVNVPTVDD45 pKa = 3.53VNVPAVDD52 pKa = 4.08LVNIPTVDD60 pKa = 3.58VNVPTVDD67 pKa = 3.51VNVPAVDD74 pKa = 3.76VNVPTVNVNVPAIDD88 pKa = 3.57INVPTVDD95 pKa = 4.25LVNIPTVDD103 pKa = 3.54VNVPTVNVNVPAVDD117 pKa = 3.76VNVPTVDD124 pKa = 3.51LVNNPTVDD132 pKa = 3.65VNVPTVDD139 pKa = 3.51LVNNPTVDD147 pKa = 3.63VNVPTVNVNVPAVDD161 pKa = 3.37INVPAVDD168 pKa = 3.88VNVPTVDD175 pKa = 3.66LVNNPAVDD183 pKa = 3.92VNVPTVDD190 pKa = 3.51LVNNPTVGHH199 pKa = 6.77

Molecular weight: 21.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C1GQW1|C1GQW1_PARBA Acetyltransferase OS=Paracoccidioides lutzii (strain ATCC MYA-826 / Pb01) OX=502779 GN=PAAG_00906 PE=4 SV=2
MM1 pKa = 7.01QGSYY5 pKa = 10.01RR6 pKa = 11.84QNSWHH11 pKa = 6.05SEE13 pKa = 3.58VRR15 pKa = 11.84FVEE18 pKa = 4.46EE19 pKa = 3.95GCLAGRR25 pKa = 11.84SKK27 pKa = 10.63GTGGGRR33 pKa = 11.84TAKK36 pKa = 10.14SAHH39 pKa = 6.66CCHH42 pKa = 5.82QQSAVEE48 pKa = 4.12QPNFAAVLAWNTARR62 pKa = 11.84TRR64 pKa = 11.84LTNKK68 pKa = 8.88PDD70 pKa = 3.2EE71 pKa = 4.54AVKK74 pKa = 10.57HH75 pKa = 5.06RR76 pKa = 11.84AQMKK80 pKa = 7.8SRR82 pKa = 11.84RR83 pKa = 11.84RR84 pKa = 11.84VTEE87 pKa = 3.88LQVKK91 pKa = 9.23RR92 pKa = 11.84SLMRR96 pKa = 11.84AVFGGVTHH104 pKa = 7.26RR105 pKa = 11.84RR106 pKa = 11.84ALSPDD111 pKa = 3.31SVLFWAKK118 pKa = 10.28VGPLGTSSVWLGILGSITRR137 pKa = 11.84YY138 pKa = 9.8RR139 pKa = 11.84SYY141 pKa = 11.37TEE143 pKa = 4.91DD144 pKa = 4.43DD145 pKa = 3.47KK146 pKa = 12.28DD147 pKa = 3.41MDD149 pKa = 4.11KK150 pKa = 10.95PATTTTGLRR159 pKa = 11.84LLFLFPSMTGINHH172 pKa = 5.74QLRR175 pKa = 11.84AICLRR180 pKa = 11.84RR181 pKa = 11.84RR182 pKa = 11.84GTPHH186 pKa = 7.32ILHH189 pKa = 7.03LLLSEE194 pKa = 4.71FPPAARR200 pKa = 11.84SRR202 pKa = 11.84ASSVMINVTSIQPYY216 pKa = 7.08VASWFTPQLKK226 pKa = 10.43ACITSIPAGTVEE238 pKa = 4.81KK239 pKa = 10.71QSSCQKK245 pKa = 8.4TCVILSPTRR254 pKa = 11.84SPTHH258 pKa = 5.87STSQLQQ264 pKa = 3.21

Molecular weight: 29.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8811

0

8811

3951676

34

5834

448.5

49.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.753 ± 0.025

1.228 ± 0.011

5.517 ± 0.019

6.2 ± 0.024

3.652 ± 0.014

6.716 ± 0.024

2.482 ± 0.013

5.13 ± 0.018

5.094 ± 0.024

8.821 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.092 ± 0.01

4.043 ± 0.014

6.251 ± 0.026

4.104 ± 0.021

6.354 ± 0.024

8.901 ± 0.03

5.887 ± 0.017

5.859 ± 0.018

1.286 ± 0.009

2.629 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski