Mycobacterium virus Avani
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 107 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I3WWV8|I3WWV8_9CAUD Uncharacterized protein OS=Mycobacterium virus Avani OX=1168594 GN=81 PE=4 SV=1
MM1 pKa = 7.71 SDD3 pKa = 5.03 CLLCDD8 pKa = 3.92 HH9 pKa = 7.15 PRR11 pKa = 11.84 STHH14 pKa = 5.11 TPQCRR19 pKa = 11.84 TRR21 pKa = 11.84 LGVDD25 pKa = 2.95 ADD27 pKa = 3.66 DD28 pKa = 3.59 MTRR31 pKa = 11.84 YY32 pKa = 6.71 TQCLCPGFEE41 pKa = 4.05 GTEE44 pKa = 4.14 DD45 pKa = 3.92 GEE47 pKa = 4.23 EE48 pKa = 4.1 DD49 pKa = 3.26
Molecular weight: 5.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.989
IPC2_protein 4.279
IPC_protein 4.151
Toseland 3.961
ProMoST 4.19
Dawson 4.139
Bjellqvist 4.368
Wikipedia 4.088
Rodwell 3.986
Grimsley 3.872
Solomon 4.126
Lehninger 4.088
Nozaki 4.266
DTASelect 4.482
Thurlkill 4.012
EMBOSS 4.088
Sillero 4.279
Patrickios 0.846
IPC_peptide 4.126
IPC2_peptide 4.253
IPC2.peptide.svr19 4.221
Protein with the highest isoelectric point:
>tr|I3WWX7|I3WWX7_9CAUD Uncharacterized protein OS=Mycobacterium virus Avani OX=1168594 GN=102 PE=4 SV=1
MM1 pKa = 6.68 TQRR4 pKa = 11.84 KK5 pKa = 9.5 GGFDD9 pKa = 4.35 WIRR12 pKa = 11.84 STYY15 pKa = 9.77 RR16 pKa = 11.84 VPAKK20 pKa = 10.02 RR21 pKa = 11.84 GMRR24 pKa = 11.84 VVFDD28 pKa = 3.72 GRR30 pKa = 11.84 PGRR33 pKa = 11.84 ILSVDD38 pKa = 3.4 GPYY41 pKa = 11.37 LMLHH45 pKa = 6.91 LDD47 pKa = 3.96 SDD49 pKa = 4.41 PKK51 pKa = 10.37 NLRR54 pKa = 11.84 TRR56 pKa = 11.84 VHH58 pKa = 5.46 PTWRR62 pKa = 11.84 MEE64 pKa = 3.91 YY65 pKa = 10.61 LPP67 pKa = 4.85
Molecular weight: 7.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 9.94
IPC_protein 11.008
Toseland 10.965
ProMoST 11.052
Dawson 11.038
Bjellqvist 10.877
Wikipedia 11.374
Rodwell 11.038
Grimsley 11.096
Solomon 11.301
Lehninger 11.242
Nozaki 10.935
DTASelect 10.877
Thurlkill 10.965
EMBOSS 11.403
Sillero 10.994
Patrickios 10.804
IPC_peptide 11.301
IPC2_peptide 10.072
IPC2.peptide.svr19 8.517
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
107
0
107
17509
33
1183
163.6
17.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.914 ± 0.487
1.314 ± 0.173
6.248 ± 0.226
6.083 ± 0.351
2.924 ± 0.135
8.653 ± 0.675
2.245 ± 0.154
4.603 ± 0.186
3.53 ± 0.205
7.71 ± 0.279
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.193 ± 0.128
3.318 ± 0.187
5.814 ± 0.206
3.615 ± 0.193
6.808 ± 0.484
5.934 ± 0.372
6.362 ± 0.35
7.054 ± 0.258
2.279 ± 0.161
2.399 ± 0.109
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here