Gordonia phage PhorbesPhlower
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A516KPS1|A0A516KPS1_9CAUD DUF1508 domain-containing protein OS=Gordonia phage PhorbesPhlower OX=2596973 GN=51 PE=4 SV=1
MM1 pKa = 7.41 GVFEE5 pKa = 4.15 QVSLVVGGEE14 pKa = 4.13 HH15 pKa = 6.69 WGSMAPSAAVDD26 pKa = 3.83 LAITAYY32 pKa = 10.36 AAAQHH37 pKa = 6.84 AGDD40 pKa = 3.94 TQQ42 pKa = 3.21
Molecular weight: 4.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.074
IPC2_protein 4.52
IPC_protein 4.139
Toseland 4.012
ProMoST 4.329
Dawson 4.139
Bjellqvist 4.304
Wikipedia 4.088
Rodwell 4.012
Grimsley 3.948
Solomon 4.101
Lehninger 4.05
Nozaki 4.304
DTASelect 4.418
Thurlkill 4.088
EMBOSS 4.101
Sillero 4.279
Patrickios 1.99
IPC_peptide 4.101
IPC2_peptide 4.253
IPC2.peptide.svr19 4.173
Protein with the highest isoelectric point:
>tr|A0A516KPS7|A0A516KPS7_9CAUD Uncharacterized protein OS=Gordonia phage PhorbesPhlower OX=2596973 GN=50 PE=4 SV=1
MM1 pKa = 7.6 TDD3 pKa = 3.19 DD4 pKa = 4.5 DD5 pKa = 6.0 RR6 pKa = 11.84 RR7 pKa = 11.84 MLDD10 pKa = 3.72 LAGQRR15 pKa = 11.84 WKK17 pKa = 11.06 YY18 pKa = 10.66 AGSLEE23 pKa = 4.01 QRR25 pKa = 11.84 VRR27 pKa = 11.84 DD28 pKa = 3.59 EE29 pKa = 4.59 FGISLTRR36 pKa = 11.84 FWQKK40 pKa = 10.39 VGQLIDD46 pKa = 3.62 TEE48 pKa = 4.28 EE49 pKa = 4.33 ALAYY53 pKa = 10.43 APIVVNRR60 pKa = 11.84 LRR62 pKa = 11.84 RR63 pKa = 11.84 LRR65 pKa = 11.84 TARR68 pKa = 11.84 RR69 pKa = 11.84 RR70 pKa = 3.54
Molecular weight: 8.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.619
IPC_protein 10.804
Toseland 10.789
ProMoST 11.286
Dawson 10.862
Bjellqvist 10.716
Wikipedia 11.213
Rodwell 10.76
Grimsley 10.921
Solomon 11.155
Lehninger 11.082
Nozaki 10.774
DTASelect 10.716
Thurlkill 10.804
EMBOSS 11.242
Sillero 10.818
Patrickios 10.57
IPC_peptide 11.155
IPC2_peptide 9.926
IPC2.peptide.svr19 9.048
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
72
0
72
13191
37
1797
183.2
19.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.675 ± 0.56
0.841 ± 0.173
6.641 ± 0.256
6.08 ± 0.343
2.449 ± 0.142
8.491 ± 0.569
1.986 ± 0.212
4.859 ± 0.248
3.631 ± 0.227
7.831 ± 0.241
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.388 ± 0.16
3.108 ± 0.224
5.542 ± 0.308
3.442 ± 0.253
7.513 ± 0.438
5.648 ± 0.242
6.694 ± 0.325
6.959 ± 0.283
2.107 ± 0.15
2.115 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here