Amino acid dipepetide frequency for Gordonia phage PhorbesPhlower

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.481AlaAla: 14.481 ± 1.874
0.91AlaCys: 0.91 ± 0.339
8.036AlaAsp: 8.036 ± 0.762
9.098AlaGlu: 9.098 ± 1.03
2.729AlaPhe: 2.729 ± 0.438
9.401AlaGly: 9.401 ± 1.103
2.274AlaHis: 2.274 ± 0.412
4.852AlaIle: 4.852 ± 0.51
5.307AlaLys: 5.307 ± 0.994
9.78AlaLeu: 9.78 ± 1.02
3.563AlaMet: 3.563 ± 0.516
4.17AlaAsn: 4.17 ± 0.627
4.321AlaPro: 4.321 ± 0.624
4.246AlaGln: 4.246 ± 0.655
8.491AlaArg: 8.491 ± 0.93
5.459AlaSer: 5.459 ± 0.567
7.202AlaThr: 7.202 ± 0.774
8.188AlaVal: 8.188 ± 0.815
2.123AlaTrp: 2.123 ± 0.429
2.047AlaTyr: 2.047 ± 0.368
0.0AlaXaa: 0.0 ± 0.0
Cys
0.607CysAla: 0.607 ± 0.225
0.227CysCys: 0.227 ± 0.134
0.531CysAsp: 0.531 ± 0.213
0.379CysGlu: 0.379 ± 0.207
0.076CysPhe: 0.076 ± 0.076
1.061CysGly: 1.061 ± 0.344
0.379CysHis: 0.379 ± 0.178
0.531CysIle: 0.531 ± 0.195
0.379CysLys: 0.379 ± 0.202
0.379CysLeu: 0.379 ± 0.155
0.152CysMet: 0.152 ± 0.104
0.379CysAsn: 0.379 ± 0.193
0.455CysPro: 0.455 ± 0.203
0.531CysGln: 0.531 ± 0.29
0.682CysArg: 0.682 ± 0.277
0.607CysSer: 0.607 ± 0.231
0.379CysThr: 0.379 ± 0.161
0.227CysVal: 0.227 ± 0.128
0.379CysTrp: 0.379 ± 0.172
0.076CysTyr: 0.076 ± 0.086
0.0CysXaa: 0.0 ± 0.0
Asp
8.036AspAla: 8.036 ± 0.753
0.303AspCys: 0.303 ± 0.13
5.004AspAsp: 5.004 ± 0.911
3.791AspGlu: 3.791 ± 0.655
1.213AspPhe: 1.213 ± 0.304
5.989AspGly: 5.989 ± 0.73
1.289AspHis: 1.289 ± 0.338
3.184AspIle: 3.184 ± 0.306
1.744AspLys: 1.744 ± 0.304
7.43AspLeu: 7.43 ± 0.641
1.365AspMet: 1.365 ± 0.342
1.592AspAsn: 1.592 ± 0.325
5.231AspPro: 5.231 ± 0.668
2.502AspGln: 2.502 ± 0.551
4.776AspArg: 4.776 ± 0.628
3.184AspSer: 3.184 ± 0.407
3.791AspThr: 3.791 ± 0.48
3.108AspVal: 3.108 ± 0.601
1.289AspTrp: 1.289 ± 0.326
1.592AspTyr: 1.592 ± 0.354
0.0AspXaa: 0.0 ± 0.0
Glu
6.748GluAla: 6.748 ± 0.805
0.607GluCys: 0.607 ± 0.255
3.26GluAsp: 3.26 ± 0.511
2.957GluGlu: 2.957 ± 0.461
1.82GluPhe: 1.82 ± 0.414
2.881GluGly: 2.881 ± 0.562
1.213GluHis: 1.213 ± 0.333
3.563GluIle: 3.563 ± 0.563
2.426GluLys: 2.426 ± 0.413
6.217GluLeu: 6.217 ± 0.797
1.516GluMet: 1.516 ± 0.354
2.199GluAsn: 2.199 ± 0.401
3.336GluPro: 3.336 ± 0.524
2.426GluGln: 2.426 ± 0.423
5.383GluArg: 5.383 ± 0.783
3.715GluSer: 3.715 ± 0.586
3.715GluThr: 3.715 ± 0.538
3.791GluVal: 3.791 ± 0.455
1.82GluTrp: 1.82 ± 0.473
1.213GluTyr: 1.213 ± 0.274
0.0GluXaa: 0.0 ± 0.0
Phe
2.502PheAla: 2.502 ± 0.341
0.303PheCys: 0.303 ± 0.183
1.668PheAsp: 1.668 ± 0.319
1.971PheGlu: 1.971 ± 0.388
0.834PhePhe: 0.834 ± 0.34
3.412PheGly: 3.412 ± 0.446
0.303PheHis: 0.303 ± 0.155
0.607PheIle: 0.607 ± 0.21
0.91PheLys: 0.91 ± 0.282
1.365PheLeu: 1.365 ± 0.416
0.303PheMet: 0.303 ± 0.135
1.061PheAsn: 1.061 ± 0.349
0.455PhePro: 0.455 ± 0.178
0.834PheGln: 0.834 ± 0.266
1.516PheArg: 1.516 ± 0.301
1.289PheSer: 1.289 ± 0.45
2.426PheThr: 2.426 ± 0.399
2.047PheVal: 2.047 ± 0.476
0.455PheTrp: 0.455 ± 0.186
0.227PheTyr: 0.227 ± 0.129
0.0PheXaa: 0.0 ± 0.0
Gly
8.112GlyAla: 8.112 ± 1.511
0.682GlyCys: 0.682 ± 0.258
5.914GlyAsp: 5.914 ± 0.734
5.459GlyGlu: 5.459 ± 0.7
1.971GlyPhe: 1.971 ± 0.292
9.704GlyGly: 9.704 ± 1.951
1.44GlyHis: 1.44 ± 0.392
3.942GlyIle: 3.942 ± 0.472
2.502GlyLys: 2.502 ± 0.574
5.307GlyLeu: 5.307 ± 0.72
2.654GlyMet: 2.654 ± 0.59
2.881GlyAsn: 2.881 ± 0.472
4.17GlyPro: 4.17 ± 0.509
2.578GlyGln: 2.578 ± 0.441
4.928GlyArg: 4.928 ± 0.558
4.776GlySer: 4.776 ± 0.559
5.307GlyThr: 5.307 ± 0.587
7.506GlyVal: 7.506 ± 0.654
2.502GlyTrp: 2.502 ± 0.326
2.578GlyTyr: 2.578 ± 0.46
0.0GlyXaa: 0.0 ± 0.0
His
1.44HisAla: 1.44 ± 0.295
0.227HisCys: 0.227 ± 0.122
0.834HisAsp: 0.834 ± 0.252
1.744HisGlu: 1.744 ± 0.356
0.303HisPhe: 0.303 ± 0.178
1.289HisGly: 1.289 ± 0.306
0.682HisHis: 0.682 ± 0.251
1.137HisIle: 1.137 ± 0.248
0.682HisLys: 0.682 ± 0.221
1.668HisLeu: 1.668 ± 0.446
0.227HisMet: 0.227 ± 0.131
0.91HisAsn: 0.91 ± 0.228
1.516HisPro: 1.516 ± 0.329
0.303HisGln: 0.303 ± 0.152
1.668HisArg: 1.668 ± 0.453
1.137HisSer: 1.137 ± 0.289
1.365HisThr: 1.365 ± 0.347
1.668HisVal: 1.668 ± 0.422
0.455HisTrp: 0.455 ± 0.156
0.607HisTyr: 0.607 ± 0.194
0.0HisXaa: 0.0 ± 0.0
Ile
6.672IleAla: 6.672 ± 0.754
0.227IleCys: 0.227 ± 0.12
3.942IleAsp: 3.942 ± 0.631
3.639IleGlu: 3.639 ± 0.69
0.91IlePhe: 0.91 ± 0.227
4.397IleGly: 4.397 ± 0.645
1.137IleHis: 1.137 ± 0.307
2.123IleIle: 2.123 ± 0.308
1.668IleLys: 1.668 ± 0.371
2.881IleLeu: 2.881 ± 0.572
0.531IleMet: 0.531 ± 0.176
1.592IleAsn: 1.592 ± 0.377
2.881IlePro: 2.881 ± 0.559
1.971IleGln: 1.971 ± 0.333
3.639IleArg: 3.639 ± 0.45
2.35IleSer: 2.35 ± 0.376
2.957IleThr: 2.957 ± 0.401
4.018IleVal: 4.018 ± 0.551
0.607IleTrp: 0.607 ± 0.217
0.455IleTyr: 0.455 ± 0.194
0.0IleXaa: 0.0 ± 0.0
Lys
4.625LysAla: 4.625 ± 0.775
0.303LysCys: 0.303 ± 0.15
1.895LysAsp: 1.895 ± 0.384
0.986LysGlu: 0.986 ± 0.315
0.834LysPhe: 0.834 ± 0.24
1.668LysGly: 1.668 ± 0.393
0.758LysHis: 0.758 ± 0.172
1.137LysIle: 1.137 ± 0.318
1.744LysLys: 1.744 ± 0.371
3.108LysLeu: 3.108 ± 0.419
0.531LysMet: 0.531 ± 0.187
1.592LysAsn: 1.592 ± 0.362
2.805LysPro: 2.805 ± 0.481
2.047LysGln: 2.047 ± 0.46
3.336LysArg: 3.336 ± 0.511
2.274LysSer: 2.274 ± 0.399
2.35LysThr: 2.35 ± 0.387
2.578LysVal: 2.578 ± 0.4
0.682LysTrp: 0.682 ± 0.278
1.061LysTyr: 1.061 ± 0.235
0.0LysXaa: 0.0 ± 0.0
Leu
10.311LeuAla: 10.311 ± 0.873
0.607LeuCys: 0.607 ± 0.243
5.989LeuAsp: 5.989 ± 0.686
4.852LeuGlu: 4.852 ± 0.523
1.592LeuPhe: 1.592 ± 0.314
6.444LeuGly: 6.444 ± 0.912
0.91LeuHis: 0.91 ± 0.293
3.715LeuIle: 3.715 ± 0.431
3.184LeuLys: 3.184 ± 0.461
4.701LeuLeu: 4.701 ± 0.622
2.123LeuMet: 2.123 ± 0.369
1.895LeuAsn: 1.895 ± 0.531
4.928LeuPro: 4.928 ± 0.634
1.744LeuGln: 1.744 ± 0.382
6.899LeuArg: 6.899 ± 0.761
4.549LeuSer: 4.549 ± 0.841
5.307LeuThr: 5.307 ± 0.492
6.065LeuVal: 6.065 ± 0.816
0.986LeuTrp: 0.986 ± 0.271
1.516LeuTyr: 1.516 ± 0.331
0.0LeuXaa: 0.0 ± 0.0
Met
2.957MetAla: 2.957 ± 0.587
0.152MetCys: 0.152 ± 0.103
1.137MetAsp: 1.137 ± 0.254
0.834MetGlu: 0.834 ± 0.219
0.531MetPhe: 0.531 ± 0.208
2.35MetGly: 2.35 ± 0.767
0.227MetHis: 0.227 ± 0.139
1.365MetIle: 1.365 ± 0.274
0.834MetLys: 0.834 ± 0.199
1.744MetLeu: 1.744 ± 0.352
0.303MetMet: 0.303 ± 0.177
0.682MetAsn: 0.682 ± 0.196
1.061MetPro: 1.061 ± 0.278
0.91MetGln: 0.91 ± 0.342
1.82MetArg: 1.82 ± 0.348
2.502MetSer: 2.502 ± 0.396
2.729MetThr: 2.729 ± 0.483
0.986MetVal: 0.986 ± 0.241
0.303MetTrp: 0.303 ± 0.19
0.455MetTyr: 0.455 ± 0.207
0.0MetXaa: 0.0 ± 0.0
Asn
3.639AsnAla: 3.639 ± 0.489
0.227AsnCys: 0.227 ± 0.139
1.895AsnAsp: 1.895 ± 0.391
1.289AsnGlu: 1.289 ± 0.285
0.834AsnPhe: 0.834 ± 0.27
3.184AsnGly: 3.184 ± 0.697
0.834AsnHis: 0.834 ± 0.29
0.834AsnIle: 0.834 ± 0.284
0.986AsnLys: 0.986 ± 0.256
3.336AsnLeu: 3.336 ± 0.716
0.682AsnMet: 0.682 ± 0.205
0.758AsnAsn: 0.758 ± 0.295
2.881AsnPro: 2.881 ± 0.454
0.986AsnGln: 0.986 ± 0.295
1.516AsnArg: 1.516 ± 0.316
1.061AsnSer: 1.061 ± 0.282
2.35AsnThr: 2.35 ± 0.423
2.426AsnVal: 2.426 ± 0.355
0.531AsnTrp: 0.531 ± 0.201
0.834AsnTyr: 0.834 ± 0.286
0.0AsnXaa: 0.0 ± 0.0
Pro
6.293ProAla: 6.293 ± 0.53
0.607ProCys: 0.607 ± 0.213
4.473ProAsp: 4.473 ± 0.895
4.018ProGlu: 4.018 ± 0.535
1.44ProPhe: 1.44 ± 0.228
4.246ProGly: 4.246 ± 0.683
1.137ProHis: 1.137 ± 0.319
2.805ProIle: 2.805 ± 0.549
2.199ProLys: 2.199 ± 0.346
3.942ProLeu: 3.942 ± 0.582
0.91ProMet: 0.91 ± 0.208
1.895ProAsn: 1.895 ± 0.314
2.805ProPro: 2.805 ± 0.596
1.744ProGln: 1.744 ± 0.329
3.412ProArg: 3.412 ± 0.534
3.487ProSer: 3.487 ± 0.551
3.412ProThr: 3.412 ± 0.711
4.17ProVal: 4.17 ± 0.747
1.289ProTrp: 1.289 ± 0.304
1.137ProTyr: 1.137 ± 0.261
0.0ProXaa: 0.0 ± 0.0
Gln
4.094GlnAla: 4.094 ± 0.553
0.227GlnCys: 0.227 ± 0.181
1.668GlnAsp: 1.668 ± 0.397
1.82GlnGlu: 1.82 ± 0.324
1.213GlnPhe: 1.213 ± 0.232
2.881GlnGly: 2.881 ± 0.478
0.834GlnHis: 0.834 ± 0.222
2.199GlnIle: 2.199 ± 0.328
1.516GlnLys: 1.516 ± 0.342
3.26GlnLeu: 3.26 ± 0.627
0.91GlnMet: 0.91 ± 0.226
0.607GlnAsn: 0.607 ± 0.175
1.592GlnPro: 1.592 ± 0.292
1.895GlnGln: 1.895 ± 0.4
2.729GlnArg: 2.729 ± 0.528
1.744GlnSer: 1.744 ± 0.325
2.123GlnThr: 2.123 ± 0.515
1.668GlnVal: 1.668 ± 0.364
0.682GlnTrp: 0.682 ± 0.221
0.758GlnTyr: 0.758 ± 0.334
0.0GlnXaa: 0.0 ± 0.0
Arg
8.264ArgAla: 8.264 ± 0.943
0.91ArgCys: 0.91 ± 0.254
3.867ArgAsp: 3.867 ± 0.605
3.942ArgGlu: 3.942 ± 0.521
2.729ArgPhe: 2.729 ± 0.35
4.473ArgGly: 4.473 ± 0.58
2.047ArgHis: 2.047 ± 0.367
3.336ArgIle: 3.336 ± 0.54
3.26ArgLys: 3.26 ± 0.47
6.141ArgLeu: 6.141 ± 0.661
1.82ArgMet: 1.82 ± 0.405
2.047ArgAsn: 2.047 ± 0.362
4.397ArgPro: 4.397 ± 0.611
3.336ArgGln: 3.336 ± 0.548
7.354ArgArg: 7.354 ± 1.005
4.17ArgSer: 4.17 ± 0.606
4.701ArgThr: 4.701 ± 0.616
4.018ArgVal: 4.018 ± 0.585
2.274ArgTrp: 2.274 ± 0.492
2.047ArgTyr: 2.047 ± 0.354
0.0ArgXaa: 0.0 ± 0.0
Ser
5.61SerAla: 5.61 ± 1.098
0.379SerCys: 0.379 ± 0.171
4.094SerAsp: 4.094 ± 0.562
3.715SerGlu: 3.715 ± 0.581
1.137SerPhe: 1.137 ± 0.244
5.762SerGly: 5.762 ± 0.884
1.061SerHis: 1.061 ± 0.298
3.26SerIle: 3.26 ± 0.559
1.365SerLys: 1.365 ± 0.261
3.715SerLeu: 3.715 ± 0.48
2.502SerMet: 2.502 ± 0.35
1.44SerAsn: 1.44 ± 0.284
2.729SerPro: 2.729 ± 0.578
1.744SerGln: 1.744 ± 0.394
4.473SerArg: 4.473 ± 0.627
3.715SerSer: 3.715 ± 0.66
4.094SerThr: 4.094 ± 0.583
3.715SerVal: 3.715 ± 0.448
1.137SerTrp: 1.137 ± 0.315
0.834SerTyr: 0.834 ± 0.286
0.0SerXaa: 0.0 ± 0.0
Thr
9.325ThrAla: 9.325 ± 0.725
0.531ThrCys: 0.531 ± 0.239
4.473ThrAsp: 4.473 ± 0.429
3.26ThrGlu: 3.26 ± 0.622
1.213ThrPhe: 1.213 ± 0.306
6.293ThrGly: 6.293 ± 0.715
1.592ThrHis: 1.592 ± 0.383
4.321ThrIle: 4.321 ± 0.805
1.82ThrLys: 1.82 ± 0.28
5.08ThrLeu: 5.08 ± 0.643
0.986ThrMet: 0.986 ± 0.252
1.82ThrAsn: 1.82 ± 0.465
4.321ThrPro: 4.321 ± 0.531
1.668ThrGln: 1.668 ± 0.364
3.715ThrArg: 3.715 ± 0.576
3.942ThrSer: 3.942 ± 0.667
4.625ThrThr: 4.625 ± 0.637
4.852ThrVal: 4.852 ± 0.638
1.44ThrTrp: 1.44 ± 0.329
1.668ThrTyr: 1.668 ± 0.401
0.0ThrXaa: 0.0 ± 0.0
Val
8.87ValAla: 8.87 ± 0.866
0.379ValCys: 0.379 ± 0.173
4.473ValAsp: 4.473 ± 0.561
4.397ValGlu: 4.397 ± 0.812
1.971ValPhe: 1.971 ± 0.452
5.686ValGly: 5.686 ± 0.683
0.834ValHis: 0.834 ± 0.287
3.26ValIle: 3.26 ± 0.522
2.502ValLys: 2.502 ± 0.445
5.155ValLeu: 5.155 ± 0.626
1.44ValMet: 1.44 ± 0.305
2.123ValAsn: 2.123 ± 0.419
3.412ValPro: 3.412 ± 0.533
1.668ValGln: 1.668 ± 0.339
5.459ValArg: 5.459 ± 0.66
3.942ValSer: 3.942 ± 0.577
5.838ValThr: 5.838 ± 0.688
5.459ValVal: 5.459 ± 0.804
1.44ValTrp: 1.44 ± 0.272
1.289ValTyr: 1.289 ± 0.326
0.0ValXaa: 0.0 ± 0.0
Trp
2.199TrpAla: 2.199 ± 0.447
0.227TrpCys: 0.227 ± 0.133
1.289TrpAsp: 1.289 ± 0.262
0.91TrpGlu: 0.91 ± 0.255
0.379TrpPhe: 0.379 ± 0.152
1.592TrpGly: 1.592 ± 0.269
0.379TrpHis: 0.379 ± 0.183
1.744TrpIle: 1.744 ± 0.337
0.682TrpLys: 0.682 ± 0.228
1.668TrpLeu: 1.668 ± 0.333
0.682TrpMet: 0.682 ± 0.191
1.213TrpAsn: 1.213 ± 0.244
1.061TrpPro: 1.061 ± 0.295
0.986TrpGln: 0.986 ± 0.215
1.971TrpArg: 1.971 ± 0.488
1.061TrpSer: 1.061 ± 0.287
1.061TrpThr: 1.061 ± 0.277
1.061TrpVal: 1.061 ± 0.304
0.531TrpTrp: 0.531 ± 0.237
0.379TrpTyr: 0.379 ± 0.173
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.971TyrAla: 1.971 ± 0.339
0.379TyrCys: 0.379 ± 0.174
1.971TyrAsp: 1.971 ± 0.594
1.744TyrGlu: 1.744 ± 0.317
0.758TyrPhe: 0.758 ± 0.192
2.199TyrGly: 2.199 ± 0.334
0.531TyrHis: 0.531 ± 0.181
0.682TyrIle: 0.682 ± 0.241
0.607TyrLys: 0.607 ± 0.224
1.44TyrLeu: 1.44 ± 0.344
0.607TyrMet: 0.607 ± 0.17
0.227TyrAsn: 0.227 ± 0.125
1.061TyrPro: 1.061 ± 0.265
0.303TyrGln: 0.303 ± 0.138
1.365TyrArg: 1.365 ± 0.372
1.516TyrSer: 1.516 ± 0.357
1.213TyrThr: 1.213 ± 0.333
2.047TyrVal: 2.047 ± 0.369
0.152TyrTrp: 0.152 ± 0.112
0.379TyrTyr: 0.379 ± 0.176
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 72 proteins (13191 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski