Acinetobacter phage phiAC-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4IBW2|K4IBW2_9CAUD Uncharacterized protein OS=Acinetobacter phage phiAC-1 OX=1229760 GN=phiAC-1_0045 PE=4 SV=1
MM1 pKa = 7.81PCLFLAATVSLIDD14 pKa = 4.93LIFDD18 pKa = 4.1FKK20 pKa = 11.41CFGALDD26 pKa = 4.19GKK28 pKa = 10.66DD29 pKa = 3.76DD30 pKa = 4.04SFTFAVDD37 pKa = 4.36FDD39 pKa = 5.23LEE41 pKa = 4.24LEE43 pKa = 4.18ACFFF47 pKa = 4.62

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4HZ87|K4HZ87_9CAUD Uncharacterized protein OS=Acinetobacter phage phiAC-1 OX=1229760 GN=phiAC-1_0024 PE=4 SV=1
MM1 pKa = 7.79EE2 pKa = 4.79IAIVLIFLAYY12 pKa = 8.71MLPTIIAFFRR22 pKa = 11.84GHH24 pKa = 6.58ASRR27 pKa = 11.84WGILLMNLILGLSVVFWFWALIWSLSNKK55 pKa = 9.73GGNQTVTVVNQMNNRR70 pKa = 11.84NGG72 pKa = 3.21

Molecular weight: 8.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

13082

37

756

159.5

17.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.277 ± 0.308

1.063 ± 0.115

6.031 ± 0.26

6.054 ± 0.297

4.388 ± 0.278

7.055 ± 0.247

1.567 ± 0.139

6.88 ± 0.225

7.354 ± 0.403

8.034 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.805 ± 0.16

6.008 ± 0.31

3.195 ± 0.166

4.067 ± 0.256

3.532 ± 0.183

6.704 ± 0.234

5.802 ± 0.325

6.597 ± 0.255

1.468 ± 0.093

4.12 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski