Amino acid dipepetide frequency for Acinetobacter phage phiAC-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.351AlaAla: 5.351 ± 0.782
0.917AlaCys: 0.917 ± 0.261
3.134AlaAsp: 3.134 ± 0.46
4.205AlaGlu: 4.205 ± 0.64
3.669AlaPhe: 3.669 ± 0.574
4.587AlaGly: 4.587 ± 0.555
1.3AlaHis: 1.3 ± 0.297
4.205AlaIle: 4.205 ± 0.65
5.657AlaLys: 5.657 ± 0.881
5.81AlaLeu: 5.81 ± 0.861
2.293AlaMet: 2.293 ± 0.431
3.822AlaAsn: 3.822 ± 0.605
2.905AlaPro: 2.905 ± 0.568
3.058AlaGln: 3.058 ± 0.648
2.676AlaArg: 2.676 ± 0.421
3.899AlaSer: 3.899 ± 0.557
5.734AlaThr: 5.734 ± 0.988
5.428AlaVal: 5.428 ± 0.837
0.764AlaTrp: 0.764 ± 0.223
3.287AlaTyr: 3.287 ± 0.418
0.0AlaXaa: 0.0 ± 0.0
Cys
0.994CysAla: 0.994 ± 0.274
0.306CysCys: 0.306 ± 0.176
0.917CysAsp: 0.917 ± 0.229
0.535CysGlu: 0.535 ± 0.216
0.688CysPhe: 0.688 ± 0.29
0.841CysGly: 0.841 ± 0.29
0.0CysHis: 0.0 ± 0.0
0.841CysIle: 0.841 ± 0.275
0.994CysLys: 0.994 ± 0.348
0.994CysLeu: 0.994 ± 0.302
0.076CysMet: 0.076 ± 0.089
0.535CysAsn: 0.535 ± 0.222
0.382CysPro: 0.382 ± 0.184
0.306CysGln: 0.306 ± 0.154
0.306CysArg: 0.306 ± 0.162
0.612CysSer: 0.612 ± 0.206
0.459CysThr: 0.459 ± 0.145
0.612CysVal: 0.612 ± 0.246
0.153CysTrp: 0.153 ± 0.094
0.076CysTyr: 0.076 ± 0.069
0.0CysXaa: 0.0 ± 0.0
Asp
4.434AspAla: 4.434 ± 0.562
0.459AspCys: 0.459 ± 0.188
3.746AspAsp: 3.746 ± 0.653
3.593AspGlu: 3.593 ± 0.608
3.44AspPhe: 3.44 ± 0.483
5.198AspGly: 5.198 ± 0.724
0.535AspHis: 0.535 ± 0.192
3.517AspIle: 3.517 ± 0.613
4.969AspLys: 4.969 ± 0.781
4.969AspLeu: 4.969 ± 0.603
1.376AspMet: 1.376 ± 0.318
2.905AspAsn: 2.905 ± 0.47
1.452AspPro: 1.452 ± 0.285
2.293AspGln: 2.293 ± 0.33
1.835AspArg: 1.835 ± 0.451
4.74AspSer: 4.74 ± 0.631
2.37AspThr: 2.37 ± 0.362
4.281AspVal: 4.281 ± 0.386
0.994AspTrp: 0.994 ± 0.263
3.211AspTyr: 3.211 ± 0.442
0.0AspXaa: 0.0 ± 0.0
Glu
3.593GluAla: 3.593 ± 0.534
0.764GluCys: 0.764 ± 0.31
3.287GluAsp: 3.287 ± 0.857
2.981GluGlu: 2.981 ± 0.55
2.905GluPhe: 2.905 ± 0.565
2.599GluGly: 2.599 ± 0.611
0.688GluHis: 0.688 ± 0.219
4.893GluIle: 4.893 ± 0.539
5.428GluLys: 5.428 ± 0.734
6.422GluLeu: 6.422 ± 0.713
2.217GluMet: 2.217 ± 0.479
3.287GluAsn: 3.287 ± 0.652
1.682GluPro: 1.682 ± 0.362
2.981GluGln: 2.981 ± 0.576
2.37GluArg: 2.37 ± 0.384
4.434GluSer: 4.434 ± 0.537
3.287GluThr: 3.287 ± 0.431
3.822GluVal: 3.822 ± 0.487
0.841GluTrp: 0.841 ± 0.24
2.064GluTyr: 2.064 ± 0.324
0.0GluXaa: 0.0 ± 0.0
Phe
2.37PheAla: 2.37 ± 0.443
0.764PheCys: 0.764 ± 0.229
4.587PheAsp: 4.587 ± 0.71
2.905PheGlu: 2.905 ± 0.448
1.605PhePhe: 1.605 ± 0.397
3.44PheGly: 3.44 ± 0.448
0.459PheHis: 0.459 ± 0.187
2.523PheIle: 2.523 ± 0.445
2.752PheLys: 2.752 ± 0.379
2.905PheLeu: 2.905 ± 0.41
0.994PheMet: 0.994 ± 0.242
3.058PheAsn: 3.058 ± 0.478
1.3PhePro: 1.3 ± 0.332
1.529PheGln: 1.529 ± 0.429
1.682PheArg: 1.682 ± 0.292
2.981PheSer: 2.981 ± 0.53
2.217PheThr: 2.217 ± 0.468
2.905PheVal: 2.905 ± 0.532
0.612PheTrp: 0.612 ± 0.237
2.293PheTyr: 2.293 ± 0.42
0.0PheXaa: 0.0 ± 0.0
Gly
4.893GlyAla: 4.893 ± 0.709
1.07GlyCys: 1.07 ± 0.284
2.446GlyAsp: 2.446 ± 0.387
4.663GlyGlu: 4.663 ± 0.598
4.052GlyPhe: 4.052 ± 0.606
5.351GlyGly: 5.351 ± 0.694
1.223GlyHis: 1.223 ± 0.33
4.663GlyIle: 4.663 ± 0.59
4.587GlyLys: 4.587 ± 0.675
6.88GlyLeu: 6.88 ± 0.608
2.523GlyMet: 2.523 ± 0.501
3.44GlyAsn: 3.44 ± 0.533
0.076GlyPro: 0.076 ± 0.087
2.752GlyGln: 2.752 ± 0.464
1.911GlyArg: 1.911 ± 0.462
5.275GlySer: 5.275 ± 0.738
4.51GlyThr: 4.51 ± 0.71
5.81GlyVal: 5.81 ± 0.723
1.07GlyTrp: 1.07 ± 0.294
3.364GlyTyr: 3.364 ± 0.523
0.0GlyXaa: 0.0 ± 0.0
His
0.994HisAla: 0.994 ± 0.273
0.229HisCys: 0.229 ± 0.108
0.917HisAsp: 0.917 ± 0.281
1.07HisGlu: 1.07 ± 0.274
0.688HisPhe: 0.688 ± 0.303
1.3HisGly: 1.3 ± 0.389
0.382HisHis: 0.382 ± 0.152
1.07HisIle: 1.07 ± 0.285
0.994HisLys: 0.994 ± 0.268
1.758HisLeu: 1.758 ± 0.331
0.229HisMet: 0.229 ± 0.132
0.917HisAsn: 0.917 ± 0.311
0.535HisPro: 0.535 ± 0.2
0.459HisGln: 0.459 ± 0.201
0.612HisArg: 0.612 ± 0.285
0.917HisSer: 0.917 ± 0.265
0.764HisThr: 0.764 ± 0.307
0.917HisVal: 0.917 ± 0.27
0.153HisTrp: 0.153 ± 0.096
0.764HisTyr: 0.764 ± 0.228
0.0HisXaa: 0.0 ± 0.0
Ile
5.428IleAla: 5.428 ± 0.772
0.764IleCys: 0.764 ± 0.206
4.893IleAsp: 4.893 ± 0.706
5.275IleGlu: 5.275 ± 0.684
1.758IlePhe: 1.758 ± 0.348
5.351IleGly: 5.351 ± 0.553
1.376IleHis: 1.376 ± 0.349
3.822IleIle: 3.822 ± 0.516
6.804IleLys: 6.804 ± 0.642
4.205IleLeu: 4.205 ± 0.515
1.911IleMet: 1.911 ± 0.43
3.593IleAsn: 3.593 ± 0.506
2.981IlePro: 2.981 ± 0.482
2.905IleGln: 2.905 ± 0.527
1.682IleArg: 1.682 ± 0.294
3.899IleSer: 3.899 ± 0.536
4.357IleThr: 4.357 ± 0.524
4.205IleVal: 4.205 ± 0.571
0.994IleTrp: 0.994 ± 0.266
2.599IleTyr: 2.599 ± 0.494
0.0IleXaa: 0.0 ± 0.0
Lys
5.963LysAla: 5.963 ± 0.805
0.612LysCys: 0.612 ± 0.266
4.281LysAsp: 4.281 ± 0.579
4.893LysGlu: 4.893 ± 0.738
1.988LysPhe: 1.988 ± 0.385
5.198LysGly: 5.198 ± 0.724
1.147LysHis: 1.147 ± 0.299
5.734LysIle: 5.734 ± 0.655
5.428LysLys: 5.428 ± 0.835
6.116LysLeu: 6.116 ± 0.738
2.523LysMet: 2.523 ± 0.487
4.893LysAsn: 4.893 ± 0.627
2.905LysPro: 2.905 ± 0.521
2.676LysGln: 2.676 ± 0.431
2.752LysArg: 2.752 ± 0.498
4.663LysSer: 4.663 ± 0.665
2.981LysThr: 2.981 ± 0.441
4.205LysVal: 4.205 ± 0.595
1.07LysTrp: 1.07 ± 0.308
3.517LysTyr: 3.517 ± 0.645
0.0LysXaa: 0.0 ± 0.0
Leu
6.498LeuAla: 6.498 ± 0.819
0.612LeuCys: 0.612 ± 0.246
4.74LeuAsp: 4.74 ± 0.63
6.192LeuGlu: 6.192 ± 0.638
3.058LeuPhe: 3.058 ± 0.501
4.893LeuGly: 4.893 ± 0.517
1.223LeuHis: 1.223 ± 0.303
6.116LeuIle: 6.116 ± 0.718
6.88LeuLys: 6.88 ± 0.735
4.052LeuLeu: 4.052 ± 0.572
1.911LeuMet: 1.911 ± 0.479
6.498LeuAsn: 6.498 ± 0.857
3.058LeuPro: 3.058 ± 0.542
1.911LeuGln: 1.911 ± 0.482
3.822LeuArg: 3.822 ± 0.467
5.428LeuSer: 5.428 ± 0.72
4.816LeuThr: 4.816 ± 0.654
5.351LeuVal: 5.351 ± 0.487
0.535LeuTrp: 0.535 ± 0.247
2.752LeuTyr: 2.752 ± 0.502
0.0LeuXaa: 0.0 ± 0.0
Met
1.988MetAla: 1.988 ± 0.392
0.306MetCys: 0.306 ± 0.172
1.988MetAsp: 1.988 ± 0.549
1.223MetGlu: 1.223 ± 0.327
0.459MetPhe: 0.459 ± 0.159
1.835MetGly: 1.835 ± 0.492
0.459MetHis: 0.459 ± 0.215
2.293MetIle: 2.293 ± 0.406
2.217MetLys: 2.217 ± 0.45
2.446MetLeu: 2.446 ± 0.328
0.382MetMet: 0.382 ± 0.162
2.217MetAsn: 2.217 ± 0.485
0.994MetPro: 0.994 ± 0.262
1.376MetGln: 1.376 ± 0.371
1.147MetArg: 1.147 ± 0.299
2.293MetSer: 2.293 ± 0.383
2.064MetThr: 2.064 ± 0.327
1.3MetVal: 1.3 ± 0.329
0.306MetTrp: 0.306 ± 0.176
0.764MetTyr: 0.764 ± 0.258
0.0MetXaa: 0.0 ± 0.0
Asn
4.357AsnAla: 4.357 ± 0.749
0.612AsnCys: 0.612 ± 0.233
2.981AsnAsp: 2.981 ± 0.402
2.752AsnGlu: 2.752 ± 0.465
2.752AsnPhe: 2.752 ± 0.459
5.504AsnGly: 5.504 ± 0.678
0.841AsnHis: 0.841 ± 0.245
4.205AsnIle: 4.205 ± 0.561
3.058AsnLys: 3.058 ± 0.41
4.587AsnLeu: 4.587 ± 0.682
0.994AsnMet: 0.994 ± 0.318
3.287AsnAsn: 3.287 ± 0.457
3.058AsnPro: 3.058 ± 0.517
3.134AsnGln: 3.134 ± 0.582
1.911AsnArg: 1.911 ± 0.375
3.899AsnSer: 3.899 ± 0.576
4.434AsnThr: 4.434 ± 0.567
4.052AsnVal: 4.052 ± 0.617
0.917AsnTrp: 0.917 ± 0.277
2.752AsnTyr: 2.752 ± 0.392
0.0AsnXaa: 0.0 ± 0.0
Pro
1.682ProAla: 1.682 ± 0.381
0.306ProCys: 0.306 ± 0.175
2.599ProAsp: 2.599 ± 0.421
2.523ProGlu: 2.523 ± 0.486
1.3ProPhe: 1.3 ± 0.378
0.306ProGly: 0.306 ± 0.147
0.459ProHis: 0.459 ± 0.206
2.829ProIle: 2.829 ± 0.451
3.058ProLys: 3.058 ± 0.574
2.523ProLeu: 2.523 ± 0.459
0.917ProMet: 0.917 ± 0.267
2.293ProAsn: 2.293 ± 0.375
0.841ProPro: 0.841 ± 0.233
1.376ProGln: 1.376 ± 0.295
0.535ProArg: 0.535 ± 0.198
2.446ProSer: 2.446 ± 0.445
2.523ProThr: 2.523 ± 0.406
1.835ProVal: 1.835 ± 0.385
0.153ProTrp: 0.153 ± 0.112
1.452ProTyr: 1.452 ± 0.326
0.0ProXaa: 0.0 ± 0.0
Gln
3.211GlnAla: 3.211 ± 0.621
0.382GlnCys: 0.382 ± 0.17
2.37GlnAsp: 2.37 ± 0.459
2.217GlnGlu: 2.217 ± 0.439
2.217GlnPhe: 2.217 ± 0.417
2.599GlnGly: 2.599 ± 0.523
0.841GlnHis: 0.841 ± 0.236
2.523GlnIle: 2.523 ± 0.453
1.911GlnLys: 1.911 ± 0.458
4.357GlnLeu: 4.357 ± 0.691
1.376GlnMet: 1.376 ± 0.354
2.064GlnAsn: 2.064 ± 0.429
1.452GlnPro: 1.452 ± 0.35
1.529GlnGln: 1.529 ± 0.443
1.529GlnArg: 1.529 ± 0.299
3.44GlnSer: 3.44 ± 0.56
2.217GlnThr: 2.217 ± 0.49
2.217GlnVal: 2.217 ± 0.39
0.764GlnTrp: 0.764 ± 0.242
1.452GlnTyr: 1.452 ± 0.366
0.0GlnXaa: 0.0 ± 0.0
Arg
2.293ArgAla: 2.293 ± 0.438
0.306ArgCys: 0.306 ± 0.146
1.911ArgAsp: 1.911 ± 0.373
2.676ArgGlu: 2.676 ± 0.523
1.605ArgPhe: 1.605 ± 0.323
2.676ArgGly: 2.676 ± 0.382
0.535ArgHis: 0.535 ± 0.195
2.676ArgIle: 2.676 ± 0.447
3.058ArgLys: 3.058 ± 0.482
2.446ArgLeu: 2.446 ± 0.503
0.688ArgMet: 0.688 ± 0.217
1.911ArgAsn: 1.911 ± 0.422
0.688ArgPro: 0.688 ± 0.175
1.758ArgGln: 1.758 ± 0.371
0.994ArgArg: 0.994 ± 0.257
2.37ArgSer: 2.37 ± 0.462
1.376ArgThr: 1.376 ± 0.334
2.905ArgVal: 2.905 ± 0.47
0.764ArgTrp: 0.764 ± 0.254
1.682ArgTyr: 1.682 ± 0.393
0.0ArgXaa: 0.0 ± 0.0
Ser
3.899SerAla: 3.899 ± 0.526
0.612SerCys: 0.612 ± 0.231
4.128SerAsp: 4.128 ± 0.508
3.669SerGlu: 3.669 ± 0.563
2.905SerPhe: 2.905 ± 0.471
5.734SerGly: 5.734 ± 0.712
0.917SerHis: 0.917 ± 0.244
5.275SerIle: 5.275 ± 0.653
4.74SerLys: 4.74 ± 0.594
5.886SerLeu: 5.886 ± 0.662
2.37SerMet: 2.37 ± 0.433
4.969SerAsn: 4.969 ± 0.575
1.911SerPro: 1.911 ± 0.357
2.599SerGln: 2.599 ± 0.376
2.446SerArg: 2.446 ± 0.383
4.51SerSer: 4.51 ± 0.517
3.211SerThr: 3.211 ± 0.436
4.052SerVal: 4.052 ± 0.537
0.917SerTrp: 0.917 ± 0.291
2.293SerTyr: 2.293 ± 0.454
0.0SerXaa: 0.0 ± 0.0
Thr
4.969ThrAla: 4.969 ± 0.627
0.535ThrCys: 0.535 ± 0.194
3.746ThrAsp: 3.746 ± 0.46
2.523ThrGlu: 2.523 ± 0.422
2.905ThrPhe: 2.905 ± 0.424
4.51ThrGly: 4.51 ± 0.645
1.529ThrHis: 1.529 ± 0.328
4.281ThrIle: 4.281 ± 0.581
2.293ThrLys: 2.293 ± 0.371
4.893ThrLeu: 4.893 ± 0.847
1.758ThrMet: 1.758 ± 0.33
2.599ThrAsn: 2.599 ± 0.607
2.064ThrPro: 2.064 ± 0.347
3.287ThrGln: 3.287 ± 0.609
1.758ThrArg: 1.758 ± 0.366
3.211ThrSer: 3.211 ± 0.483
3.593ThrThr: 3.593 ± 0.675
4.205ThrVal: 4.205 ± 0.607
1.529ThrTrp: 1.529 ± 0.366
1.835ThrTyr: 1.835 ± 0.352
0.0ThrXaa: 0.0 ± 0.0
Val
5.428ValAla: 5.428 ± 0.644
0.612ValCys: 0.612 ± 0.213
4.052ValAsp: 4.052 ± 0.551
3.593ValGlu: 3.593 ± 0.605
3.287ValPhe: 3.287 ± 0.481
4.205ValGly: 4.205 ± 0.601
0.764ValHis: 0.764 ± 0.253
3.975ValIle: 3.975 ± 0.589
4.893ValLys: 4.893 ± 0.695
4.969ValLeu: 4.969 ± 0.651
1.605ValMet: 1.605 ± 0.339
3.822ValAsn: 3.822 ± 0.665
1.758ValPro: 1.758 ± 0.323
2.217ValGln: 2.217 ± 0.364
2.676ValArg: 2.676 ± 0.286
3.975ValSer: 3.975 ± 0.446
4.587ValThr: 4.587 ± 0.73
4.816ValVal: 4.816 ± 0.906
1.605ValTrp: 1.605 ± 0.297
3.134ValTyr: 3.134 ± 0.439
0.0ValXaa: 0.0 ± 0.0
Trp
1.376TrpAla: 1.376 ± 0.299
0.306TrpCys: 0.306 ± 0.143
0.841TrpAsp: 0.841 ± 0.258
0.306TrpGlu: 0.306 ± 0.161
1.07TrpPhe: 1.07 ± 0.244
1.147TrpGly: 1.147 ± 0.319
0.306TrpHis: 0.306 ± 0.148
1.07TrpIle: 1.07 ± 0.341
0.841TrpLys: 0.841 ± 0.279
1.07TrpLeu: 1.07 ± 0.291
0.229TrpMet: 0.229 ± 0.143
0.917TrpAsn: 0.917 ± 0.214
0.153TrpPro: 0.153 ± 0.092
0.612TrpGln: 0.612 ± 0.22
1.07TrpArg: 1.07 ± 0.301
0.994TrpSer: 0.994 ± 0.29
0.459TrpThr: 0.459 ± 0.164
0.841TrpVal: 0.841 ± 0.242
0.229TrpTrp: 0.229 ± 0.115
0.841TrpTyr: 0.841 ± 0.243
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.058TyrAla: 3.058 ± 0.558
0.153TyrCys: 0.153 ± 0.11
2.752TyrAsp: 2.752 ± 0.428
2.752TyrGlu: 2.752 ± 0.495
1.529TyrPhe: 1.529 ± 0.276
3.287TyrGly: 3.287 ± 0.586
0.688TyrHis: 0.688 ± 0.262
2.293TyrIle: 2.293 ± 0.349
2.981TyrLys: 2.981 ± 0.528
3.058TyrLeu: 3.058 ± 0.584
1.605TyrMet: 1.605 ± 0.41
3.058TyrAsn: 3.058 ± 0.495
1.758TyrPro: 1.758 ± 0.418
1.911TyrGln: 1.911 ± 0.418
1.605TyrArg: 1.605 ± 0.41
3.058TyrSer: 3.058 ± 0.56
2.064TyrThr: 2.064 ± 0.43
2.217TyrVal: 2.217 ± 0.454
0.306TyrTrp: 0.306 ± 0.157
1.07TyrTyr: 1.07 ± 0.238
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 82 proteins (13082 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski