European elk papillomavirus (EEPV)
Average proteome isoelectric point is 7.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P11331|VE6_PAPVE Protein E6 OS=European elk papillomavirus OX=10565 GN=E6 PE=3 SV=1
MM1 pKa = 7.77 TYY3 pKa = 11.23 GLLLFLGLTFGLQLMLLVFLLFFFLVWWDD32 pKa = 3.56 QFGCRR37 pKa = 11.84 CEE39 pKa = 4.24 NMQLL43 pKa = 3.46
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.526
IPC2_protein 5.067
IPC_protein 4.19
Toseland 4.062
ProMoST 4.406
Dawson 4.19
Bjellqvist 4.368
Wikipedia 4.19
Rodwell 4.062
Grimsley 4.012
Solomon 4.113
Lehninger 4.075
Nozaki 4.393
DTASelect 4.444
Thurlkill 4.19
EMBOSS 4.19
Sillero 4.317
Patrickios 0.299
IPC_peptide 4.113
IPC2_peptide 4.279
IPC2.peptide.svr19 4.257
Protein with the highest isoelectric point:
>tr|Q84259|Q84259_PAPVE Uncharacterized protein E4 (Fragment) OS=European elk papillomavirus OX=10565 GN=E4 PE=4 SV=1
MM1 pKa = 7.4 GCYY4 pKa = 9.55 HH5 pKa = 7.28 PLIIVSFITLKK16 pKa = 10.55 DD17 pKa = 3.05 ACYY20 pKa = 10.21 LPKK23 pKa = 10.48 TRR25 pKa = 11.84 DD26 pKa = 3.61 VQSLEE31 pKa = 3.85 QLVHH35 pKa = 6.44 HH36 pKa = 7.07 GGHH39 pKa = 5.49 VAVTSRR45 pKa = 11.84 LVNGFQMSLPRR56 pKa = 11.84 PTATSRR62 pKa = 11.84 LYY64 pKa = 9.06 LTPGFRR70 pKa = 11.84 RR71 pKa = 11.84 TINFMITRR79 pKa = 11.84 CSHH82 pKa = 5.79 GVSPTCVKK90 pKa = 10.53 KK91 pKa = 10.22 PSPNHH96 pKa = 6.91 RR97 pKa = 11.84 ISLWRR102 pKa = 11.84 RR103 pKa = 11.84 DD104 pKa = 3.51 SDD106 pKa = 3.64 SGKK109 pKa = 9.62 PRR111 pKa = 11.84 TKK113 pKa = 10.79 LLDD116 pKa = 3.52 LL117 pKa = 4.89
Molecular weight: 13.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.338
IPC2_protein 9.56
IPC_protein 10.072
Toseland 10.467
ProMoST 10.116
Dawson 10.599
Bjellqvist 10.292
Wikipedia 10.774
Rodwell 10.891
Grimsley 10.643
Solomon 10.672
Lehninger 10.643
Nozaki 10.496
DTASelect 10.277
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.526
Patrickios 10.657
IPC_peptide 10.672
IPC2_peptide 9.502
IPC2.peptide.svr19 8.454
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15
0
15
2914
24
611
194.3
21.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.589 ± 0.655
2.78 ± 0.641
4.633 ± 0.635
5.319 ± 0.506
3.844 ± 0.417
7.344 ± 0.734
2.814 ± 0.453
4.049 ± 0.537
5.045 ± 0.679
9.369 ± 0.924
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.922 ± 0.402
2.917 ± 0.504
7.104 ± 0.781
3.912 ± 0.297
7.241 ± 0.896
7.756 ± 0.616
6.658 ± 0.412
6.143 ± 0.346
1.613 ± 0.334
2.951 ± 0.272
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here