Shahe tombus-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG67|A0A1L3KG67_9VIRU Uncharacterized protein OS=Shahe tombus-like virus 2 OX=1923456 PE=4 SV=1
MM1 pKa = 7.25EE2 pKa = 5.14QFICSYY8 pKa = 10.65KK9 pKa = 10.14GAKK12 pKa = 7.95QHH14 pKa = 5.24TVRR17 pKa = 11.84AAAEE21 pKa = 4.0DD22 pKa = 4.25VLRR25 pKa = 11.84HH26 pKa = 4.98PAVRR30 pKa = 11.84RR31 pKa = 11.84DD32 pKa = 3.53AKK34 pKa = 10.95LKK36 pKa = 9.82TFVKK40 pKa = 10.52AEE42 pKa = 3.94KK43 pKa = 10.48LNLSSKK49 pKa = 9.85PDD51 pKa = 3.65PAPRR55 pKa = 11.84VIQPRR60 pKa = 11.84DD61 pKa = 3.16VRR63 pKa = 11.84YY64 pKa = 8.53NCEE67 pKa = 3.54VGPYY71 pKa = 9.88LKK73 pKa = 10.6AHH75 pKa = 6.01EE76 pKa = 4.09HH77 pKa = 5.56TIYY80 pKa = 10.67RR81 pKa = 11.84GIAKK85 pKa = 9.48IWGGPTVMKK94 pKa = 10.53GFNSVDD100 pKa = 3.38TAKK103 pKa = 10.5HH104 pKa = 4.72MRR106 pKa = 11.84EE107 pKa = 4.02MWDD110 pKa = 4.38SIHH113 pKa = 7.11DD114 pKa = 3.73PCGIGLDD121 pKa = 3.45ASRR124 pKa = 11.84FDD126 pKa = 3.33QHH128 pKa = 7.84VSEE131 pKa = 4.65EE132 pKa = 4.04ALRR135 pKa = 11.84WEE137 pKa = 4.0HH138 pKa = 6.29SVYY141 pKa = 11.32NGMFKK146 pKa = 10.46SKK148 pKa = 10.46RR149 pKa = 11.84LAEE152 pKa = 4.12LLTWQINNVGTSYY165 pKa = 10.32TPEE168 pKa = 3.56GRR170 pKa = 11.84VTYY173 pKa = 9.83KK174 pKa = 10.97VKK176 pKa = 9.91GCRR179 pKa = 11.84MSGDD183 pKa = 3.68MNTSLGNCLIMCGMVWEE200 pKa = 5.2LCQQLGCPARR210 pKa = 11.84LANNGDD216 pKa = 3.82DD217 pKa = 4.47CMLIVPRR224 pKa = 11.84SWEE227 pKa = 3.43QRR229 pKa = 11.84VRR231 pKa = 11.84DD232 pKa = 4.7KK233 pKa = 10.83IASWFLDD240 pKa = 3.47FGFTMKK246 pKa = 10.55VEE248 pKa = 3.99DD249 pKa = 3.54TVYY252 pKa = 10.43EE253 pKa = 4.07FEE255 pKa = 5.08QIEE258 pKa = 4.63FCQTRR263 pKa = 11.84PVFASNRR270 pKa = 11.84WVMTRR275 pKa = 11.84DD276 pKa = 3.27YY277 pKa = 10.65RR278 pKa = 11.84VVMDD282 pKa = 4.99KK283 pKa = 10.58DD284 pKa = 4.19TFCLHH289 pKa = 7.44PDD291 pKa = 3.06NMPYY295 pKa = 10.22EE296 pKa = 3.97RR297 pKa = 11.84WLRR300 pKa = 11.84GVGTAGLALASGVPVLQTFYY320 pKa = 10.0TQLVALGGGQGEE332 pKa = 5.01LIDD335 pKa = 4.33GSGMSYY341 pKa = 8.8MAKK344 pKa = 10.39GLTPDD349 pKa = 4.06ASPITTEE356 pKa = 4.1ARR358 pKa = 11.84VSFYY362 pKa = 10.79KK363 pKa = 10.79AFGMPPWVQEE373 pKa = 4.03AYY375 pKa = 9.99EE376 pKa = 4.08EE377 pKa = 4.61EE378 pKa = 4.48IVASASSTNLGLIGSRR394 pKa = 11.84YY395 pKa = 7.53TYY397 pKa = 10.88HH398 pKa = 6.89NNTLAFQGMTGKK410 pKa = 8.88MKK412 pKa = 10.62KK413 pKa = 9.98PSTQKK418 pKa = 11.24SMIRR422 pKa = 11.84NIPIDD427 pKa = 3.92VPGGPPGYY435 pKa = 9.5TNFQRR440 pKa = 11.84KK441 pKa = 6.43TGPRR445 pKa = 11.84VTATKK450 pKa = 10.6AGLQIANSEE459 pKa = 4.08QFLPLSADD467 pKa = 3.32SSSNTVVSVGLNPADD482 pKa = 3.57ANLFRR487 pKa = 11.84WLSGIARR494 pKa = 11.84RR495 pKa = 11.84YY496 pKa = 7.87TMYY499 pKa = 10.1RR500 pKa = 11.84WKK502 pKa = 10.33KK503 pKa = 10.42LRR505 pKa = 11.84VLYY508 pKa = 10.61QSTCPTTTPGSVHH521 pKa = 6.94LGLFYY526 pKa = 10.92DD527 pKa = 5.85AEE529 pKa = 4.52DD530 pKa = 3.91LGNWIADD537 pKa = 3.53GSSTVTLSQTVGASIGPTWGSTMTSTNSGSITTNMVEE574 pKa = 3.96VDD576 pKa = 3.29VTRR579 pKa = 11.84AHH581 pKa = 6.5QRR583 pKa = 11.84VSWHH587 pKa = 6.81LIDD590 pKa = 5.25GNTGGTAVDD599 pKa = 3.94NQSVAALCASVVTPVPGGAVVGRR622 pKa = 11.84IWYY625 pKa = 9.3DD626 pKa = 3.3YY627 pKa = 10.34EE628 pKa = 5.14IEE630 pKa = 4.92LIQPTFAFGGNGLRR644 pKa = 11.84GGAGARR650 pKa = 11.84GFDD653 pKa = 3.79PSKK656 pKa = 10.77DD657 pKa = 3.26IYY659 pKa = 9.53LTKK662 pKa = 9.8PDD664 pKa = 4.25YY665 pKa = 11.41VPVPRR670 pKa = 11.84PLPKK674 pKa = 9.11PTPSGDD680 pKa = 3.37DD681 pKa = 3.46DD682 pKa = 4.17EE683 pKa = 5.3EE684 pKa = 5.39RR685 pKa = 11.84EE686 pKa = 4.29PVGG689 pKa = 4.09

Molecular weight: 76.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG67|A0A1L3KG67_9VIRU Uncharacterized protein OS=Shahe tombus-like virus 2 OX=1923456 PE=4 SV=1
MM1 pKa = 7.9ADD3 pKa = 4.28FSPVPAATIGGEE15 pKa = 4.25SQSEE19 pKa = 4.22GMANPNFTFGTVEE32 pKa = 3.8VLARR36 pKa = 11.84GAVACIVLTGRR47 pKa = 11.84KK48 pKa = 8.55LSKK51 pKa = 10.04WFWPYY56 pKa = 11.01QPTAKK61 pKa = 10.56QIDD64 pKa = 4.28GLVKK68 pKa = 10.41LDD70 pKa = 4.7SEE72 pKa = 4.94DD73 pKa = 3.52NDD75 pKa = 4.03GGVSGWALTKK85 pKa = 10.09KK86 pKa = 8.78WCAVAKK92 pKa = 10.77AEE94 pKa = 4.46FGTVQKK100 pKa = 9.5RR101 pKa = 11.84TPANDD106 pKa = 2.99AAIADD111 pKa = 4.32KK112 pKa = 10.53IRR114 pKa = 11.84RR115 pKa = 11.84LMKK118 pKa = 9.92EE119 pKa = 3.53ANTRR123 pKa = 11.84NVDD126 pKa = 4.13IIRR129 pKa = 11.84VLPWAVASVYY139 pKa = 10.66VPTDD143 pKa = 3.27ADD145 pKa = 3.63LVAASVYY152 pKa = 7.6NHH154 pKa = 6.5KK155 pKa = 10.31VVKK158 pKa = 10.32ARR160 pKa = 11.84RR161 pKa = 11.84KK162 pKa = 9.75HH163 pKa = 5.42LAVPQKK169 pKa = 11.06

Molecular weight: 18.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

858

169

689

429.0

47.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.508 ± 2.699

1.748 ± 0.268

5.478 ± 0.208

4.429 ± 0.417

3.263 ± 0.145

8.741 ± 1.058

1.748 ± 0.268

4.196 ± 0.026

5.245 ± 1.441

6.643 ± 0.344

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.03 ± 0.595

3.963 ± 0.085

5.944 ± 0.293

3.38 ± 0.2

5.828 ± 0.238

6.527 ± 0.85

7.226 ± 0.621

8.392 ± 1.352

2.448 ± 0.242

3.263 ± 0.706
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski