Mycobacterium montefiorense
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5384 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2R5HH93|A0A2R5HH93_9MYCO Uncharacterized protein OS=Mycobacterium montefiorense OX=154654 GN=MmonteBS_49640 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 9.95 VTVDD6 pKa = 3.5 QNICASSGNCVMNAPEE22 pKa = 4.59 VFDD25 pKa = 4.66 QRR27 pKa = 11.84 DD28 pKa = 3.24 EE29 pKa = 4.83 DD30 pKa = 4.48 GVVEE34 pKa = 5.3 LLTEE38 pKa = 4.08 QPSADD43 pKa = 3.36 QTDD46 pKa = 4.19 GVRR49 pKa = 11.84 QAAAACPAQAIYY61 pKa = 10.32 IEE63 pKa = 4.43 EE64 pKa = 4.1
Molecular weight: 6.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.77
IPC_protein 3.656
Toseland 3.465
ProMoST 3.783
Dawson 3.656
Bjellqvist 3.91
Wikipedia 3.605
Rodwell 3.503
Grimsley 3.389
Solomon 3.617
Lehninger 3.567
Nozaki 3.795
DTASelect 3.948
Thurlkill 3.541
EMBOSS 3.605
Sillero 3.783
Patrickios 1.837
IPC_peptide 3.605
IPC2_peptide 3.745
IPC2.peptide.svr19 3.772
Protein with the highest isoelectric point:
>tr|A0A2R5HD44|A0A2R5HD44_9MYCO LuxR family transcriptional regulator OS=Mycobacterium montefiorense OX=154654 GN=MmonteBS_34340 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 VHH19 pKa = 5.99 GFRR22 pKa = 11.84 LRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVSGRR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.66 GRR42 pKa = 11.84 RR43 pKa = 11.84 ALSAA47 pKa = 3.69
Molecular weight: 5.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.374
IPC_protein 12.983
Toseland 13.144
ProMoST 13.642
Dawson 13.144
Bjellqvist 13.144
Wikipedia 13.627
Rodwell 12.735
Grimsley 13.188
Solomon 13.642
Lehninger 13.554
Nozaki 13.144
DTASelect 13.144
Thurlkill 13.144
EMBOSS 13.642
Sillero 13.144
Patrickios 12.457
IPC_peptide 13.656
IPC2_peptide 12.632
IPC2.peptide.svr19 9.285
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5384
0
5384
1725077
39
4196
320.4
34.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.812 ± 0.053
0.866 ± 0.009
6.166 ± 0.027
5.137 ± 0.032
3.115 ± 0.018
8.773 ± 0.027
2.271 ± 0.014
4.489 ± 0.022
2.245 ± 0.024
9.869 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.134 ± 0.015
2.401 ± 0.018
5.779 ± 0.03
3.201 ± 0.018
7.034 ± 0.028
5.651 ± 0.021
5.903 ± 0.018
8.427 ± 0.024
1.518 ± 0.013
2.199 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here