Roseovarius albus
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4169 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X6ZTS5|A0A1X6ZTS5_9RHOB Aminopeptidase N OS=Roseovarius albus OX=1247867 GN=pepN PE=3 SV=1
MM1 pKa = 7.42 HH2 pKa = 7.97 PNRR5 pKa = 11.84 LSVFIASMMLMPVAGVAEE23 pKa = 4.7 DD24 pKa = 4.5 DD25 pKa = 3.91 SHH27 pKa = 8.62 FLITGEE33 pKa = 4.09 RR34 pKa = 11.84 LACVMSHH41 pKa = 5.9 SADD44 pKa = 3.45 YY45 pKa = 11.3 SFDD48 pKa = 3.6 SGDD51 pKa = 3.35 VAFIAVEE58 pKa = 4.19 DD59 pKa = 4.23 CSGDD63 pKa = 3.14 GGGQVSLANMVLNSAPDD80 pKa = 3.31 VSVADD85 pKa = 4.26 DD86 pKa = 3.64 QAPDD90 pKa = 3.43 AVVALSKK97 pKa = 11.24 DD98 pKa = 3.63 DD99 pKa = 4.33 LACLANLDD107 pKa = 3.89 VAGADD112 pKa = 3.36 ALYY115 pKa = 10.85 AFYY118 pKa = 10.49 PNGCRR123 pKa = 11.84 LEE125 pKa = 4.21 QKK127 pKa = 10.47 DD128 pKa = 3.44
Molecular weight: 13.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.795
IPC_protein 3.795
Toseland 3.567
ProMoST 3.935
Dawson 3.808
Bjellqvist 4.012
Wikipedia 3.795
Rodwell 3.617
Grimsley 3.478
Solomon 3.795
Lehninger 3.757
Nozaki 3.935
DTASelect 4.228
Thurlkill 3.643
EMBOSS 3.795
Sillero 3.923
Patrickios 0.947
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|A0A1X6YV72|A0A1X6YV72_9RHOB Uncharacterized protein OS=Roseovarius albus OX=1247867 GN=ROA7450_01415 PE=4 SV=1
MM1 pKa = 7.7 VEE3 pKa = 3.81 AAPRR7 pKa = 11.84 KK8 pKa = 8.43 VWAKK12 pKa = 9.97 RR13 pKa = 11.84 VLYY16 pKa = 10.37 LALCFTLIFLHH27 pKa = 6.91 LLPLDD32 pKa = 3.41 MLAFRR37 pKa = 11.84 GISEE41 pKa = 4.19 NAGDD45 pKa = 4.51 LIEE48 pKa = 4.73 PRR50 pKa = 11.84 LLRR53 pKa = 11.84 PIRR56 pKa = 11.84 WAGPDD61 pKa = 3.37 LMLAFTLAWVRR72 pKa = 11.84 RR73 pKa = 11.84 RR74 pKa = 11.84 PDD76 pKa = 3.89 YY77 pKa = 11.02 IPAILVAFVFLLADD91 pKa = 4.52 LLFQRR96 pKa = 11.84 PPGLLSVIVVLACEE110 pKa = 4.19 LQRR113 pKa = 11.84 RR114 pKa = 11.84 RR115 pKa = 11.84 TMLFRR120 pKa = 11.84 TQPLLVEE127 pKa = 4.59 WFNVAIVVAAIALANRR143 pKa = 11.84 LVLSVTFVDD152 pKa = 4.88 QVPLGLNLMQVIATVLVYY170 pKa = 9.86 PLVVFATQVIFGVRR184 pKa = 11.84 RR185 pKa = 11.84 TTKK188 pKa = 10.52 KK189 pKa = 10.54 DD190 pKa = 2.88 IAAMGVGII198 pKa = 4.93
Molecular weight: 22.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.589
IPC_protein 10.628
Toseland 10.672
ProMoST 10.511
Dawson 10.774
Bjellqvist 10.555
Wikipedia 11.038
Rodwell 10.804
Grimsley 10.818
Solomon 10.95
Lehninger 10.906
Nozaki 10.672
DTASelect 10.54
Thurlkill 10.672
EMBOSS 11.096
Sillero 10.716
Patrickios 10.54
IPC_peptide 10.95
IPC2_peptide 9.78
IPC2.peptide.svr19 8.601
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4169
0
4169
1280323
31
4734
307.1
33.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.819 ± 0.048
1.022 ± 0.013
6.029 ± 0.035
6.221 ± 0.042
3.936 ± 0.027
8.297 ± 0.09
2.186 ± 0.024
5.624 ± 0.028
3.775 ± 0.029
9.782 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.81 ± 0.021
3.191 ± 0.022
4.76 ± 0.029
3.511 ± 0.019
5.859 ± 0.041
5.738 ± 0.036
5.47 ± 0.027
7.068 ± 0.032
1.428 ± 0.016
2.476 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here