Salmonella phage GJL01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tempevirinae; Tlsvirus; unclassified Tlsvirus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V0DZ42|A0A1V0DZ42_9CAUD Putative DNA cytosine methyltransferase OS=Salmonella phage GJL01 OX=1965464 GN=GJL01_64 PE=4 SV=1
MM1 pKa = 7.94KK2 pKa = 10.17YY3 pKa = 10.04QIYY6 pKa = 10.22SAIDD10 pKa = 4.4AIDD13 pKa = 4.28DD14 pKa = 4.03AQSLFMFDD22 pKa = 4.17EE23 pKa = 4.4NDD25 pKa = 3.42NYY27 pKa = 11.13TVVCDD32 pKa = 4.75CYY34 pKa = 11.03TDD36 pKa = 4.17ADD38 pKa = 4.19YY39 pKa = 11.65LEE41 pKa = 4.02QLARR45 pKa = 11.84NGEE48 pKa = 4.14GCNWSDD54 pKa = 4.45NKK56 pKa = 11.0NEE58 pKa = 4.14TPRR61 pKa = 11.84RR62 pKa = 11.84QDD64 pKa = 2.95MINPVLVKK72 pKa = 10.43EE73 pKa = 4.48FEE75 pKa = 4.25VV76 pKa = 3.55

Molecular weight: 8.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V0DZ56|A0A1V0DZ56_9CAUD Putative polyribonucleotide nucleotidyltransferase OS=Salmonella phage GJL01 OX=1965464 GN=GJL01_62 PE=4 SV=1
MM1 pKa = 7.37FCPIRR6 pKa = 11.84FNGVDD11 pKa = 3.64LLKK14 pKa = 10.3EE15 pKa = 4.09SRR17 pKa = 11.84MCTTAIVNRR26 pKa = 11.84LYY28 pKa = 10.91LRR30 pKa = 11.84SGGNIVKK37 pKa = 10.48LEE39 pKa = 3.91YY40 pKa = 10.08RR41 pKa = 11.84SGRR44 pKa = 11.84IIRR47 pKa = 11.84TDD49 pKa = 2.97TGRR52 pKa = 11.84WIEE55 pKa = 4.14TNEE58 pKa = 4.1LLWHH62 pKa = 5.66NAKK65 pKa = 10.09GRR67 pKa = 11.84LLFKK71 pKa = 10.22TFEE74 pKa = 5.07DD75 pKa = 3.57IRR77 pKa = 5.28

Molecular weight: 9.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

14047

35

1258

175.6

19.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.144 ± 0.391

1.402 ± 0.171

6.229 ± 0.257

7.254 ± 0.253

3.944 ± 0.162

7.062 ± 0.209

1.794 ± 0.216

6.663 ± 0.199

7.468 ± 0.391

6.948 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.161 ± 0.197

4.941 ± 0.231

3.218 ± 0.184

3.958 ± 0.285

4.962 ± 0.219

5.51 ± 0.239

5.503 ± 0.219

6.685 ± 0.279

1.402 ± 0.117

3.752 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski