Amino acid dipepetide frequency for Salmonella phage GJL01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.977AlaAla: 6.977 ± 1.084
0.712AlaCys: 0.712 ± 0.238
4.485AlaAsp: 4.485 ± 0.592
5.624AlaGlu: 5.624 ± 0.731
2.99AlaPhe: 2.99 ± 0.478
5.98AlaGly: 5.98 ± 0.747
0.783AlaHis: 0.783 ± 0.261
5.055AlaIle: 5.055 ± 0.567
7.262AlaLys: 7.262 ± 1.149
7.76AlaLeu: 7.76 ± 0.697
2.919AlaMet: 2.919 ± 0.495
4.129AlaAsn: 4.129 ± 0.729
1.709AlaPro: 1.709 ± 0.304
4.343AlaGln: 4.343 ± 0.6
3.987AlaArg: 3.987 ± 0.617
3.916AlaSer: 3.916 ± 0.624
3.56AlaThr: 3.56 ± 0.639
5.34AlaVal: 5.34 ± 0.693
0.712AlaTrp: 0.712 ± 0.195
3.204AlaTyr: 3.204 ± 0.478
0.0AlaXaa: 0.0 ± 0.0
Cys
1.068CysAla: 1.068 ± 0.324
0.142CysCys: 0.142 ± 0.091
1.139CysAsp: 1.139 ± 0.223
0.854CysGlu: 0.854 ± 0.267
0.285CysPhe: 0.285 ± 0.158
1.21CysGly: 1.21 ± 0.332
0.356CysHis: 0.356 ± 0.166
0.641CysIle: 0.641 ± 0.236
0.641CysLys: 0.641 ± 0.233
0.926CysLeu: 0.926 ± 0.279
0.854CysMet: 0.854 ± 0.248
0.783CysAsn: 0.783 ± 0.242
0.498CysPro: 0.498 ± 0.176
0.356CysGln: 0.356 ± 0.193
0.854CysArg: 0.854 ± 0.304
0.57CysSer: 0.57 ± 0.167
0.997CysThr: 0.997 ± 0.306
0.926CysVal: 0.926 ± 0.241
0.356CysTrp: 0.356 ± 0.158
0.57CysTyr: 0.57 ± 0.203
0.0CysXaa: 0.0 ± 0.0
Asp
4.77AspAla: 4.77 ± 0.647
0.641AspCys: 0.641 ± 0.216
3.631AspAsp: 3.631 ± 0.525
4.628AspGlu: 4.628 ± 0.488
2.848AspPhe: 2.848 ± 0.433
5.197AspGly: 5.197 ± 0.934
1.139AspHis: 1.139 ± 0.234
3.489AspIle: 3.489 ± 0.386
4.77AspLys: 4.77 ± 0.476
4.841AspLeu: 4.841 ± 0.546
1.495AspMet: 1.495 ± 0.315
3.702AspAsn: 3.702 ± 0.514
2.492AspPro: 2.492 ± 0.421
1.78AspGln: 1.78 ± 0.338
2.777AspArg: 2.777 ± 0.542
2.777AspSer: 2.777 ± 0.422
3.417AspThr: 3.417 ± 0.416
3.417AspVal: 3.417 ± 0.555
0.926AspTrp: 0.926 ± 0.244
3.56AspTyr: 3.56 ± 0.465
0.0AspXaa: 0.0 ± 0.0
Glu
5.482GluAla: 5.482 ± 0.697
1.424GluCys: 1.424 ± 0.341
3.204GluAsp: 3.204 ± 0.582
4.699GluGlu: 4.699 ± 0.714
3.845GluPhe: 3.845 ± 0.591
3.417GluGly: 3.417 ± 0.414
1.495GluHis: 1.495 ± 0.318
4.556GluIle: 4.556 ± 0.635
5.34GluLys: 5.34 ± 0.617
5.34GluLeu: 5.34 ± 0.633
2.278GluMet: 2.278 ± 0.379
4.272GluAsn: 4.272 ± 0.45
2.207GluPro: 2.207 ± 0.466
4.129GluGln: 4.129 ± 0.652
3.204GluArg: 3.204 ± 0.527
4.414GluSer: 4.414 ± 0.523
4.414GluThr: 4.414 ± 0.542
4.841GluVal: 4.841 ± 0.581
0.854GluTrp: 0.854 ± 0.289
3.133GluTyr: 3.133 ± 0.415
0.0GluXaa: 0.0 ± 0.0
Phe
2.99PheAla: 2.99 ± 0.504
0.498PheCys: 0.498 ± 0.201
3.346PheAsp: 3.346 ± 0.529
2.634PheGlu: 2.634 ± 0.523
0.997PhePhe: 0.997 ± 0.242
3.133PheGly: 3.133 ± 0.623
0.427PheHis: 0.427 ± 0.164
2.634PheIle: 2.634 ± 0.549
2.99PheLys: 2.99 ± 0.431
2.207PheLeu: 2.207 ± 0.338
1.139PheMet: 1.139 ± 0.294
2.563PheAsn: 2.563 ± 0.286
1.78PhePro: 1.78 ± 0.379
1.282PheGln: 1.282 ± 0.337
1.353PheArg: 1.353 ± 0.3
2.136PheSer: 2.136 ± 0.451
3.133PheThr: 3.133 ± 0.57
2.634PheVal: 2.634 ± 0.394
0.427PheTrp: 0.427 ± 0.179
1.139PheTyr: 1.139 ± 0.362
0.0PheXaa: 0.0 ± 0.0
Gly
4.556GlyAla: 4.556 ± 0.733
1.282GlyCys: 1.282 ± 0.329
4.2GlyAsp: 4.2 ± 0.632
5.909GlyGlu: 5.909 ± 0.601
2.777GlyPhe: 2.777 ± 0.465
6.479GlyGly: 6.479 ± 0.781
1.139GlyHis: 1.139 ± 0.289
4.058GlyIle: 4.058 ± 0.501
5.909GlyLys: 5.909 ± 0.582
4.841GlyLeu: 4.841 ± 0.453
2.919GlyMet: 2.919 ± 0.394
3.275GlyAsn: 3.275 ± 0.373
0.071GlyPro: 0.071 ± 0.08
1.993GlyGln: 1.993 ± 0.461
2.848GlyArg: 2.848 ± 0.404
5.055GlySer: 5.055 ± 0.647
3.489GlyThr: 3.489 ± 0.543
5.268GlyVal: 5.268 ± 0.55
1.424GlyTrp: 1.424 ± 0.259
3.133GlyTyr: 3.133 ± 0.42
0.0GlyXaa: 0.0 ± 0.0
His
1.139HisAla: 1.139 ± 0.259
0.142HisCys: 0.142 ± 0.104
1.21HisAsp: 1.21 ± 0.357
0.854HisGlu: 0.854 ± 0.246
0.783HisPhe: 0.783 ± 0.263
1.78HisGly: 1.78 ± 0.401
0.712HisHis: 0.712 ± 0.285
1.068HisIle: 1.068 ± 0.294
1.21HisLys: 1.21 ± 0.414
1.495HisLeu: 1.495 ± 0.431
0.356HisMet: 0.356 ± 0.149
0.641HisAsn: 0.641 ± 0.274
0.641HisPro: 0.641 ± 0.276
0.854HisGln: 0.854 ± 0.265
0.997HisArg: 0.997 ± 0.307
1.068HisSer: 1.068 ± 0.309
0.997HisThr: 0.997 ± 0.254
1.21HisVal: 1.21 ± 0.291
0.142HisTrp: 0.142 ± 0.103
0.641HisTyr: 0.641 ± 0.244
0.0HisXaa: 0.0 ± 0.0
Ile
6.408IleAla: 6.408 ± 0.652
0.783IleCys: 0.783 ± 0.215
5.34IleAsp: 5.34 ± 0.595
5.197IleGlu: 5.197 ± 0.692
2.349IlePhe: 2.349 ± 0.412
3.489IleGly: 3.489 ± 0.471
1.353IleHis: 1.353 ± 0.279
3.987IleIle: 3.987 ± 0.5
4.984IleLys: 4.984 ± 0.557
3.275IleLeu: 3.275 ± 0.509
2.136IleMet: 2.136 ± 0.443
3.702IleAsn: 3.702 ± 0.643
2.634IlePro: 2.634 ± 0.422
1.922IleGln: 1.922 ± 0.405
2.919IleArg: 2.919 ± 0.476
4.343IleSer: 4.343 ± 0.572
4.984IleThr: 4.984 ± 0.546
3.773IleVal: 3.773 ± 0.451
0.854IleTrp: 0.854 ± 0.261
2.207IleTyr: 2.207 ± 0.364
0.0IleXaa: 0.0 ± 0.0
Lys
7.333LysAla: 7.333 ± 0.733
0.783LysCys: 0.783 ± 0.261
4.343LysAsp: 4.343 ± 0.524
7.191LysGlu: 7.191 ± 0.95
2.065LysPhe: 2.065 ± 0.349
3.987LysGly: 3.987 ± 0.527
1.424LysHis: 1.424 ± 0.341
4.628LysIle: 4.628 ± 0.496
4.556LysLys: 4.556 ± 0.642
5.34LysLeu: 5.34 ± 0.521
3.061LysMet: 3.061 ± 0.471
3.631LysAsn: 3.631 ± 0.47
3.133LysPro: 3.133 ± 0.493
2.492LysGln: 2.492 ± 0.482
4.2LysArg: 4.2 ± 0.678
3.773LysSer: 3.773 ± 0.531
4.414LysThr: 4.414 ± 0.591
4.699LysVal: 4.699 ± 0.557
0.997LysTrp: 0.997 ± 0.306
2.634LysTyr: 2.634 ± 0.495
0.0LysXaa: 0.0 ± 0.0
Leu
7.191LeuAla: 7.191 ± 1.107
1.282LeuCys: 1.282 ± 0.321
3.061LeuAsp: 3.061 ± 0.418
4.343LeuGlu: 4.343 ± 0.589
2.278LeuPhe: 2.278 ± 0.406
3.346LeuGly: 3.346 ± 0.557
1.282LeuHis: 1.282 ± 0.33
4.77LeuIle: 4.77 ± 0.524
5.268LeuLys: 5.268 ± 0.677
4.272LeuLeu: 4.272 ± 0.513
2.065LeuMet: 2.065 ± 0.459
4.129LeuAsn: 4.129 ± 0.577
2.99LeuPro: 2.99 ± 0.44
1.922LeuGln: 1.922 ± 0.389
3.702LeuArg: 3.702 ± 0.512
4.058LeuSer: 4.058 ± 0.478
5.767LeuThr: 5.767 ± 0.765
4.414LeuVal: 4.414 ± 0.49
1.282LeuTrp: 1.282 ± 0.297
1.993LeuTyr: 1.993 ± 0.423
0.0LeuXaa: 0.0 ± 0.0
Met
2.563MetAla: 2.563 ± 0.43
0.57MetCys: 0.57 ± 0.196
1.78MetAsp: 1.78 ± 0.432
1.21MetGlu: 1.21 ± 0.327
1.566MetPhe: 1.566 ± 0.384
1.353MetGly: 1.353 ± 0.291
0.57MetHis: 0.57 ± 0.167
3.061MetIle: 3.061 ± 0.482
2.421MetLys: 2.421 ± 0.463
2.634MetLeu: 2.634 ± 0.462
1.353MetMet: 1.353 ± 0.347
1.353MetAsn: 1.353 ± 0.277
0.997MetPro: 0.997 ± 0.284
1.993MetGln: 1.993 ± 0.379
1.993MetArg: 1.993 ± 0.327
1.851MetSer: 1.851 ± 0.434
1.495MetThr: 1.495 ± 0.316
1.851MetVal: 1.851 ± 0.328
0.285MetTrp: 0.285 ± 0.143
0.712MetTyr: 0.712 ± 0.209
0.0MetXaa: 0.0 ± 0.0
Asn
4.485AsnAla: 4.485 ± 0.539
0.498AsnCys: 0.498 ± 0.187
3.417AsnAsp: 3.417 ± 0.457
3.773AsnGlu: 3.773 ± 0.556
1.993AsnPhe: 1.993 ± 0.346
4.841AsnGly: 4.841 ± 0.614
0.926AsnHis: 0.926 ± 0.277
3.417AsnIle: 3.417 ± 0.513
4.058AsnLys: 4.058 ± 0.542
2.705AsnLeu: 2.705 ± 0.442
0.854AsnMet: 0.854 ± 0.29
2.563AsnAsn: 2.563 ± 0.446
1.709AsnPro: 1.709 ± 0.372
1.993AsnGln: 1.993 ± 0.398
2.278AsnArg: 2.278 ± 0.379
2.634AsnSer: 2.634 ± 0.507
1.78AsnThr: 1.78 ± 0.303
3.346AsnVal: 3.346 ± 0.447
0.641AsnTrp: 0.641 ± 0.181
1.495AsnTyr: 1.495 ± 0.317
0.0AsnXaa: 0.0 ± 0.0
Pro
2.349ProAla: 2.349 ± 0.403
0.427ProCys: 0.427 ± 0.211
2.349ProAsp: 2.349 ± 0.515
3.346ProGlu: 3.346 ± 0.652
1.282ProPhe: 1.282 ± 0.291
2.421ProGly: 2.421 ± 0.423
0.641ProHis: 0.641 ± 0.224
2.207ProIle: 2.207 ± 0.469
1.495ProLys: 1.495 ± 0.34
1.566ProLeu: 1.566 ± 0.251
0.854ProMet: 0.854 ± 0.275
1.709ProAsn: 1.709 ± 0.324
1.21ProPro: 1.21 ± 0.38
1.424ProGln: 1.424 ± 0.305
1.353ProArg: 1.353 ± 0.326
1.495ProSer: 1.495 ± 0.302
1.495ProThr: 1.495 ± 0.266
2.919ProVal: 2.919 ± 0.433
0.285ProTrp: 0.285 ± 0.132
1.353ProTyr: 1.353 ± 0.294
0.0ProXaa: 0.0 ± 0.0
Gln
2.99GlnAla: 2.99 ± 0.466
0.498GlnCys: 0.498 ± 0.165
2.705GlnAsp: 2.705 ± 0.473
2.777GlnGlu: 2.777 ± 0.438
1.637GlnPhe: 1.637 ± 0.359
1.566GlnGly: 1.566 ± 0.39
0.712GlnHis: 0.712 ± 0.224
3.133GlnIle: 3.133 ± 0.505
2.705GlnLys: 2.705 ± 0.553
3.702GlnLeu: 3.702 ± 0.634
1.068GlnMet: 1.068 ± 0.279
1.495GlnAsn: 1.495 ± 0.364
1.353GlnPro: 1.353 ± 0.304
2.705GlnGln: 2.705 ± 0.756
2.421GlnArg: 2.421 ± 0.413
2.207GlnSer: 2.207 ± 0.49
1.709GlnThr: 1.709 ± 0.421
2.207GlnVal: 2.207 ± 0.422
0.356GlnTrp: 0.356 ± 0.131
1.637GlnTyr: 1.637 ± 0.29
0.0GlnXaa: 0.0 ± 0.0
Arg
3.987ArgAla: 3.987 ± 0.562
0.926ArgCys: 0.926 ± 0.38
2.349ArgAsp: 2.349 ± 0.432
3.631ArgGlu: 3.631 ± 0.445
2.207ArgPhe: 2.207 ± 0.374
3.417ArgGly: 3.417 ± 0.489
0.712ArgHis: 0.712 ± 0.24
3.916ArgIle: 3.916 ± 0.489
4.129ArgLys: 4.129 ± 0.584
2.99ArgLeu: 2.99 ± 0.526
1.709ArgMet: 1.709 ± 0.366
1.709ArgAsn: 1.709 ± 0.37
1.424ArgPro: 1.424 ± 0.402
1.78ArgGln: 1.78 ± 0.422
3.417ArgArg: 3.417 ± 0.622
2.349ArgSer: 2.349 ± 0.431
1.709ArgThr: 1.709 ± 0.372
4.485ArgVal: 4.485 ± 0.649
0.641ArgTrp: 0.641 ± 0.214
2.136ArgTyr: 2.136 ± 0.448
0.0ArgXaa: 0.0 ± 0.0
Ser
4.485SerAla: 4.485 ± 0.612
0.641SerCys: 0.641 ± 0.224
4.2SerAsp: 4.2 ± 0.619
4.556SerGlu: 4.556 ± 0.564
1.993SerPhe: 1.993 ± 0.291
5.553SerGly: 5.553 ± 0.625
0.712SerHis: 0.712 ± 0.289
4.628SerIle: 4.628 ± 0.717
3.489SerLys: 3.489 ± 0.55
3.275SerLeu: 3.275 ± 0.522
1.566SerMet: 1.566 ± 0.32
1.709SerAsn: 1.709 ± 0.372
1.495SerPro: 1.495 ± 0.367
2.207SerGln: 2.207 ± 0.536
2.634SerArg: 2.634 ± 0.47
2.207SerSer: 2.207 ± 0.448
2.492SerThr: 2.492 ± 0.505
3.916SerVal: 3.916 ± 0.523
1.139SerTrp: 1.139 ± 0.267
2.207SerTyr: 2.207 ± 0.365
0.0SerXaa: 0.0 ± 0.0
Thr
4.343ThrAla: 4.343 ± 0.711
0.427ThrCys: 0.427 ± 0.204
3.489ThrAsp: 3.489 ± 0.502
3.061ThrGlu: 3.061 ± 0.556
3.275ThrPhe: 3.275 ± 0.391
5.34ThrGly: 5.34 ± 0.464
0.926ThrHis: 0.926 ± 0.266
3.133ThrIle: 3.133 ± 0.335
3.916ThrLys: 3.916 ± 0.731
3.916ThrLeu: 3.916 ± 0.511
1.495ThrMet: 1.495 ± 0.276
2.207ThrAsn: 2.207 ± 0.392
2.563ThrPro: 2.563 ± 0.413
2.136ThrGln: 2.136 ± 0.483
1.993ThrArg: 1.993 ± 0.391
2.919ThrSer: 2.919 ± 0.551
2.207ThrThr: 2.207 ± 0.368
4.485ThrVal: 4.485 ± 0.739
1.21ThrTrp: 1.21 ± 0.234
1.993ThrTyr: 1.993 ± 0.408
0.0ThrXaa: 0.0 ± 0.0
Val
3.845ValAla: 3.845 ± 0.48
1.353ValCys: 1.353 ± 0.347
4.414ValAsp: 4.414 ± 0.619
5.34ValGlu: 5.34 ± 0.677
2.136ValPhe: 2.136 ± 0.462
4.129ValGly: 4.129 ± 0.464
1.068ValHis: 1.068 ± 0.286
4.984ValIle: 4.984 ± 0.591
6.123ValLys: 6.123 ± 0.909
4.556ValLeu: 4.556 ± 0.488
2.563ValMet: 2.563 ± 0.414
3.061ValAsn: 3.061 ± 0.511
2.065ValPro: 2.065 ± 0.362
1.922ValGln: 1.922 ± 0.292
3.56ValArg: 3.56 ± 0.55
3.987ValSer: 3.987 ± 0.63
4.2ValThr: 4.2 ± 0.46
4.058ValVal: 4.058 ± 0.537
1.139ValTrp: 1.139 ± 0.271
2.349ValTyr: 2.349 ± 0.373
0.0ValXaa: 0.0 ± 0.0
Trp
0.997TrpAla: 0.997 ± 0.306
0.285TrpCys: 0.285 ± 0.161
0.712TrpAsp: 0.712 ± 0.209
0.854TrpGlu: 0.854 ± 0.281
0.641TrpPhe: 0.641 ± 0.25
1.139TrpGly: 1.139 ± 0.25
0.427TrpHis: 0.427 ± 0.15
0.997TrpIle: 0.997 ± 0.234
0.854TrpLys: 0.854 ± 0.227
1.353TrpLeu: 1.353 ± 0.281
0.214TrpMet: 0.214 ± 0.127
0.926TrpAsn: 0.926 ± 0.199
0.214TrpPro: 0.214 ± 0.121
0.356TrpGln: 0.356 ± 0.147
1.068TrpArg: 1.068 ± 0.316
0.783TrpSer: 0.783 ± 0.314
0.926TrpThr: 0.926 ± 0.201
0.783TrpVal: 0.783 ± 0.2
0.142TrpTrp: 0.142 ± 0.097
0.356TrpTyr: 0.356 ± 0.124
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.489TyrAla: 3.489 ± 0.655
0.712TyrCys: 0.712 ± 0.225
2.919TyrAsp: 2.919 ± 0.369
2.065TyrGlu: 2.065 ± 0.439
1.495TyrPhe: 1.495 ± 0.344
2.848TyrGly: 2.848 ± 0.43
1.139TyrHis: 1.139 ± 0.26
2.278TyrIle: 2.278 ± 0.361
2.563TyrLys: 2.563 ± 0.422
2.492TyrLeu: 2.492 ± 0.378
0.641TyrMet: 0.641 ± 0.195
1.851TyrAsn: 1.851 ± 0.38
0.997TyrPro: 0.997 ± 0.268
1.993TyrGln: 1.993 ± 0.338
2.065TyrArg: 2.065 ± 0.506
2.563TyrSer: 2.563 ± 0.483
1.851TyrThr: 1.851 ± 0.298
2.278TyrVal: 2.278 ± 0.374
0.214TyrTrp: 0.214 ± 0.103
1.068TyrTyr: 1.068 ± 0.333
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (14047 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski