Paracoccus phage Shpa

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vhulanivirus; Paracoccus virus Shpa

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U2BX41|A0A0U2BX41_9CAUD Lysozyme-like protein OS=Paracoccus phage Shpa OX=1647282 GN=Shpa_27 PE=3 SV=1
MM1 pKa = 7.84TDD3 pKa = 3.18QIDD6 pKa = 3.66NGGPAFPTAPGQMAHH21 pKa = 6.29NSGMTLRR28 pKa = 11.84DD29 pKa = 3.21WFAGQALVGMTADD42 pKa = 5.91GGDD45 pKa = 3.36VWNDD49 pKa = 3.29AVWNDD54 pKa = 3.85TPRR57 pKa = 11.84EE58 pKa = 3.94AAMRR62 pKa = 11.84AASLCYY68 pKa = 10.33GIADD72 pKa = 3.17AMLAVRR78 pKa = 11.84GGGAAA83 pKa = 3.5

Molecular weight: 8.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U2C177|A0A0U2C177_9CAUD Uncharacterized protein OS=Paracoccus phage Shpa OX=1647282 GN=Shpa_52 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 9.61ATINGRR8 pKa = 11.84RR9 pKa = 11.84FDD11 pKa = 3.37TSTARR16 pKa = 11.84KK17 pKa = 8.6LAEE20 pKa = 4.12VSHH23 pKa = 6.09GQPGDD28 pKa = 3.23PGRR31 pKa = 11.84YY32 pKa = 8.95VEE34 pKa = 4.9TLYY37 pKa = 8.83QTPRR41 pKa = 11.84SRR43 pKa = 11.84AYY45 pKa = 10.39FLACEE50 pKa = 4.63GGPGSRR56 pKa = 11.84YY57 pKa = 9.41AVSVRR62 pKa = 11.84PGEE65 pKa = 3.69WRR67 pKa = 11.84AGHH70 pKa = 7.1KK71 pKa = 8.37ITPLTAEE78 pKa = 4.47DD79 pKa = 3.48AMRR82 pKa = 11.84WAAAAGRR89 pKa = 11.84QGYY92 pKa = 9.9GSLSVSRR99 pKa = 11.84ALGMTEE105 pKa = 4.75KK106 pKa = 10.68NALPIMRR113 pKa = 11.84RR114 pKa = 11.84GGWNGEE120 pKa = 4.02RR121 pKa = 11.84NAA123 pKa = 4.51

Molecular weight: 13.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11640

55

1072

207.9

22.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.393 ± 0.549

0.962 ± 0.18

6.151 ± 0.224

5.507 ± 0.378

3.084 ± 0.217

8.84 ± 0.342

1.993 ± 0.19

5.12 ± 0.296

3.385 ± 0.364

7.543 ± 0.438

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.809 ± 0.226

2.784 ± 0.283

5.206 ± 0.333

3.866 ± 0.23

8.239 ± 0.3

5.43 ± 0.309

5.55 ± 0.51

6.203 ± 0.271

2.01 ± 0.153

1.924 ± 0.123
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski