Amino acid dipepetide frequency for Paracoccus phage Shpa

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.136AlaAla: 21.136 ± 1.981
1.547AlaCys: 1.547 ± 0.401
7.475AlaAsp: 7.475 ± 0.988
8.935AlaGlu: 8.935 ± 1.614
4.725AlaPhe: 4.725 ± 0.866
9.623AlaGly: 9.623 ± 0.752
2.492AlaHis: 2.492 ± 0.459
6.702AlaIle: 6.702 ± 0.727
5.499AlaLys: 5.499 ± 0.78
12.544AlaLeu: 12.544 ± 1.017
4.296AlaMet: 4.296 ± 0.542
3.093AlaAsn: 3.093 ± 0.614
5.928AlaPro: 5.928 ± 0.719
4.725AlaGln: 4.725 ± 0.547
9.021AlaArg: 9.021 ± 1.078
6.1AlaSer: 6.1 ± 0.893
7.131AlaThr: 7.131 ± 1.052
8.506AlaVal: 8.506 ± 1.101
1.976AlaTrp: 1.976 ± 0.427
2.406AlaTyr: 2.406 ± 0.441
0.0AlaXaa: 0.0 ± 0.0
Cys
1.203CysAla: 1.203 ± 0.326
0.172CysCys: 0.172 ± 0.111
0.773CysAsp: 0.773 ± 0.208
0.516CysGlu: 0.516 ± 0.194
0.086CysPhe: 0.086 ± 0.073
0.687CysGly: 0.687 ± 0.273
0.43CysHis: 0.43 ± 0.208
0.258CysIle: 0.258 ± 0.138
0.344CysLys: 0.344 ± 0.155
0.601CysLeu: 0.601 ± 0.248
0.258CysMet: 0.258 ± 0.156
0.43CysAsn: 0.43 ± 0.2
0.859CysPro: 0.859 ± 0.297
0.43CysGln: 0.43 ± 0.215
0.687CysArg: 0.687 ± 0.257
0.773CysSer: 0.773 ± 0.304
0.258CysThr: 0.258 ± 0.15
0.516CysVal: 0.516 ± 0.216
0.258CysTrp: 0.258 ± 0.17
0.086CysTyr: 0.086 ± 0.081
0.0CysXaa: 0.0 ± 0.0
Asp
8.764AspAla: 8.764 ± 0.768
0.516AspCys: 0.516 ± 0.25
5.155AspAsp: 5.155 ± 0.714
3.437AspGlu: 3.437 ± 0.533
1.547AspPhe: 1.547 ± 0.348
7.045AspGly: 7.045 ± 0.632
1.461AspHis: 1.461 ± 0.311
2.32AspIle: 2.32 ± 0.473
1.461AspLys: 1.461 ± 0.469
5.069AspLeu: 5.069 ± 0.866
1.718AspMet: 1.718 ± 0.48
1.547AspAsn: 1.547 ± 0.28
2.749AspPro: 2.749 ± 0.373
3.007AspGln: 3.007 ± 0.563
5.241AspArg: 5.241 ± 0.929
2.749AspSer: 2.749 ± 0.622
1.976AspThr: 1.976 ± 0.551
3.437AspVal: 3.437 ± 0.483
1.031AspTrp: 1.031 ± 0.333
1.289AspTyr: 1.289 ± 0.353
0.0AspXaa: 0.0 ± 0.0
Glu
8.592GluAla: 8.592 ± 1.545
0.344GluCys: 0.344 ± 0.26
2.578GluAsp: 2.578 ± 0.57
2.234GluGlu: 2.234 ± 0.522
1.976GluPhe: 1.976 ± 0.449
3.78GluGly: 3.78 ± 0.626
1.203GluHis: 1.203 ± 0.265
3.523GluIle: 3.523 ± 0.654
1.976GluLys: 1.976 ± 0.399
3.007GluLeu: 3.007 ± 0.451
2.406GluMet: 2.406 ± 0.542
1.289GluAsn: 1.289 ± 0.301
1.976GluPro: 1.976 ± 0.427
2.234GluGln: 2.234 ± 0.613
5.327GluArg: 5.327 ± 0.79
2.32GluSer: 2.32 ± 0.494
3.523GluThr: 3.523 ± 0.571
4.124GluVal: 4.124 ± 0.613
1.289GluTrp: 1.289 ± 0.309
1.375GluTyr: 1.375 ± 0.352
0.0GluXaa: 0.0 ± 0.0
Phe
4.124PheAla: 4.124 ± 0.503
0.172PheCys: 0.172 ± 0.146
2.578PheAsp: 2.578 ± 0.551
1.547PheGlu: 1.547 ± 0.431
0.945PhePhe: 0.945 ± 0.275
2.921PheGly: 2.921 ± 0.532
0.43PheHis: 0.43 ± 0.219
1.547PheIle: 1.547 ± 0.477
1.031PheLys: 1.031 ± 0.391
1.804PheLeu: 1.804 ± 0.338
0.859PheMet: 0.859 ± 0.23
0.859PheAsn: 0.859 ± 0.201
1.289PhePro: 1.289 ± 0.398
1.117PheGln: 1.117 ± 0.236
2.835PheArg: 2.835 ± 0.49
1.203PheSer: 1.203 ± 0.327
2.062PheThr: 2.062 ± 0.444
2.406PheVal: 2.406 ± 0.558
0.601PheTrp: 0.601 ± 0.232
0.516PheTyr: 0.516 ± 0.2
0.0PheXaa: 0.0 ± 0.0
Gly
9.795GlyAla: 9.795 ± 1.183
0.687GlyCys: 0.687 ± 0.267
6.186GlyAsp: 6.186 ± 0.982
5.671GlyGlu: 5.671 ± 0.918
3.093GlyPhe: 3.093 ± 0.502
8.764GlyGly: 8.764 ± 0.979
1.976GlyHis: 1.976 ± 0.462
5.327GlyIle: 5.327 ± 0.919
3.179GlyLys: 3.179 ± 0.522
7.475GlyLeu: 7.475 ± 0.984
2.406GlyMet: 2.406 ± 0.369
2.835GlyAsn: 2.835 ± 0.639
3.179GlyPro: 3.179 ± 0.489
4.038GlyGln: 4.038 ± 0.531
5.671GlyArg: 5.671 ± 0.777
4.811GlySer: 4.811 ± 0.517
3.179GlyThr: 3.179 ± 0.511
5.069GlyVal: 5.069 ± 0.663
2.062GlyTrp: 2.062 ± 0.405
3.007GlyTyr: 3.007 ± 0.589
0.0GlyXaa: 0.0 ± 0.0
His
2.32HisAla: 2.32 ± 0.374
0.172HisCys: 0.172 ± 0.107
1.203HisAsp: 1.203 ± 0.33
1.203HisGlu: 1.203 ± 0.339
0.687HisPhe: 0.687 ± 0.223
1.718HisGly: 1.718 ± 0.459
0.516HisHis: 0.516 ± 0.226
0.687HisIle: 0.687 ± 0.218
0.687HisLys: 0.687 ± 0.211
1.547HisLeu: 1.547 ± 0.45
0.516HisMet: 0.516 ± 0.193
0.43HisAsn: 0.43 ± 0.191
1.632HisPro: 1.632 ± 0.327
0.859HisGln: 0.859 ± 0.297
2.663HisArg: 2.663 ± 0.551
0.945HisSer: 0.945 ± 0.305
0.687HisThr: 0.687 ± 0.243
0.945HisVal: 0.945 ± 0.271
0.172HisTrp: 0.172 ± 0.108
0.344HisTyr: 0.344 ± 0.211
0.0HisXaa: 0.0 ± 0.0
Ile
5.757IleAla: 5.757 ± 0.761
0.687IleCys: 0.687 ± 0.266
3.78IleAsp: 3.78 ± 0.971
4.382IleGlu: 4.382 ± 0.684
0.945IlePhe: 0.945 ± 0.28
4.897IleGly: 4.897 ± 0.755
0.516IleHis: 0.516 ± 0.199
1.976IleIle: 1.976 ± 0.593
1.976IleLys: 1.976 ± 0.325
2.835IleLeu: 2.835 ± 0.396
1.289IleMet: 1.289 ± 0.311
1.375IleAsn: 1.375 ± 0.372
1.718IlePro: 1.718 ± 0.373
1.117IleGln: 1.117 ± 0.276
4.124IleArg: 4.124 ± 0.62
3.265IleSer: 3.265 ± 0.632
5.069IleThr: 5.069 ± 1.254
3.093IleVal: 3.093 ± 0.593
1.461IleTrp: 1.461 ± 0.408
0.945IleTyr: 0.945 ± 0.257
0.0IleXaa: 0.0 ± 0.0
Lys
6.186LysAla: 6.186 ± 1.016
0.258LysCys: 0.258 ± 0.164
1.289LysAsp: 1.289 ± 0.249
1.632LysGlu: 1.632 ± 0.38
1.031LysPhe: 1.031 ± 0.369
2.578LysGly: 2.578 ± 0.625
0.773LysHis: 0.773 ± 0.287
2.062LysIle: 2.062 ± 0.556
1.89LysLys: 1.89 ± 0.52
2.062LysLeu: 2.062 ± 0.536
1.031LysMet: 1.031 ± 0.33
0.687LysAsn: 0.687 ± 0.222
2.663LysPro: 2.663 ± 0.528
1.031LysGln: 1.031 ± 0.323
1.976LysArg: 1.976 ± 0.476
1.89LysSer: 1.89 ± 0.452
1.461LysThr: 1.461 ± 0.529
2.062LysVal: 2.062 ± 0.464
0.773LysTrp: 0.773 ± 0.231
0.516LysTyr: 0.516 ± 0.207
0.0LysXaa: 0.0 ± 0.0
Leu
7.561LeuAla: 7.561 ± 1.06
1.031LeuCys: 1.031 ± 0.344
5.155LeuAsp: 5.155 ± 0.793
3.265LeuGlu: 3.265 ± 0.593
1.718LeuPhe: 1.718 ± 0.304
6.186LeuGly: 6.186 ± 0.949
1.375LeuHis: 1.375 ± 0.402
4.296LeuIle: 4.296 ± 0.586
2.492LeuLys: 2.492 ± 0.566
4.811LeuLeu: 4.811 ± 0.721
1.289LeuMet: 1.289 ± 0.372
2.234LeuAsn: 2.234 ± 0.461
4.21LeuPro: 4.21 ± 0.603
2.148LeuGln: 2.148 ± 0.543
7.99LeuArg: 7.99 ± 0.757
6.014LeuSer: 6.014 ± 0.987
5.671LeuThr: 5.671 ± 0.715
5.155LeuVal: 5.155 ± 0.487
1.718LeuTrp: 1.718 ± 0.351
1.117LeuTyr: 1.117 ± 0.338
0.0LeuXaa: 0.0 ± 0.0
Met
3.179MetAla: 3.179 ± 0.563
0.43MetCys: 0.43 ± 0.207
0.945MetAsp: 0.945 ± 0.272
1.461MetGlu: 1.461 ± 0.415
0.516MetPhe: 0.516 ± 0.24
2.148MetGly: 2.148 ± 0.478
0.43MetHis: 0.43 ± 0.186
1.289MetIle: 1.289 ± 0.261
0.945MetLys: 0.945 ± 0.338
1.976MetLeu: 1.976 ± 0.369
1.031MetMet: 1.031 ± 0.314
1.117MetAsn: 1.117 ± 0.349
2.062MetPro: 2.062 ± 0.414
1.117MetGln: 1.117 ± 0.342
3.007MetArg: 3.007 ± 0.425
2.148MetSer: 2.148 ± 0.416
2.749MetThr: 2.749 ± 0.637
1.117MetVal: 1.117 ± 0.253
0.43MetTrp: 0.43 ± 0.177
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
4.468AsnAla: 4.468 ± 0.565
0.086AsnCys: 0.086 ± 0.089
1.547AsnAsp: 1.547 ± 0.392
1.461AsnGlu: 1.461 ± 0.507
0.859AsnPhe: 0.859 ± 0.222
3.523AsnGly: 3.523 ± 0.554
0.086AsnHis: 0.086 ± 0.092
1.289AsnIle: 1.289 ± 0.286
0.945AsnLys: 0.945 ± 0.302
2.32AsnLeu: 2.32 ± 0.426
0.172AsnMet: 0.172 ± 0.12
0.859AsnAsn: 0.859 ± 0.522
2.32AsnPro: 2.32 ± 0.589
0.344AsnGln: 0.344 ± 0.196
2.062AsnArg: 2.062 ± 0.408
1.031AsnSer: 1.031 ± 0.258
1.718AsnThr: 1.718 ± 0.526
1.718AsnVal: 1.718 ± 0.386
0.516AsnTrp: 0.516 ± 0.266
0.516AsnTyr: 0.516 ± 0.202
0.0AsnXaa: 0.0 ± 0.0
Pro
6.358ProAla: 6.358 ± 0.685
0.773ProCys: 0.773 ± 0.265
4.296ProAsp: 4.296 ± 0.535
3.523ProGlu: 3.523 ± 0.494
1.375ProPhe: 1.375 ± 0.323
4.811ProGly: 4.811 ± 0.76
1.031ProHis: 1.031 ± 0.314
2.062ProIle: 2.062 ± 0.391
1.375ProLys: 1.375 ± 0.393
3.952ProLeu: 3.952 ± 0.54
1.031ProMet: 1.031 ± 0.257
1.289ProAsn: 1.289 ± 0.381
3.351ProPro: 3.351 ± 0.727
2.492ProGln: 2.492 ± 0.449
4.983ProArg: 4.983 ± 0.924
2.32ProSer: 2.32 ± 0.556
2.148ProThr: 2.148 ± 0.412
3.265ProVal: 3.265 ± 0.61
1.031ProTrp: 1.031 ± 0.35
0.601ProTyr: 0.601 ± 0.2
0.0ProXaa: 0.0 ± 0.0
Gln
5.413GlnAla: 5.413 ± 0.55
0.258GlnCys: 0.258 ± 0.126
1.89GlnAsp: 1.89 ± 0.391
1.461GlnGlu: 1.461 ± 0.358
1.718GlnPhe: 1.718 ± 0.424
3.609GlnGly: 3.609 ± 0.579
0.687GlnHis: 0.687 ± 0.277
2.578GlnIle: 2.578 ± 0.553
0.773GlnLys: 0.773 ± 0.228
2.148GlnLeu: 2.148 ± 0.391
1.547GlnMet: 1.547 ± 0.417
1.117GlnAsn: 1.117 ± 0.277
1.89GlnPro: 1.89 ± 0.503
1.632GlnGln: 1.632 ± 0.415
3.437GlnArg: 3.437 ± 0.518
1.804GlnSer: 1.804 ± 0.37
1.804GlnThr: 1.804 ± 0.344
3.437GlnVal: 3.437 ± 0.45
1.031GlnTrp: 1.031 ± 0.322
0.43GlnTyr: 0.43 ± 0.156
0.0GlnXaa: 0.0 ± 0.0
Arg
10.482ArgAla: 10.482 ± 1.383
0.516ArgCys: 0.516 ± 0.182
5.585ArgAsp: 5.585 ± 0.516
5.155ArgGlu: 5.155 ± 0.673
3.179ArgPhe: 3.179 ± 0.537
5.928ArgGly: 5.928 ± 0.719
2.234ArgHis: 2.234 ± 0.529
4.21ArgIle: 4.21 ± 0.568
2.835ArgLys: 2.835 ± 0.602
7.561ArgLeu: 7.561 ± 0.992
2.749ArgMet: 2.749 ± 0.595
1.632ArgAsn: 1.632 ± 0.319
3.952ArgPro: 3.952 ± 0.639
3.007ArgGln: 3.007 ± 0.471
6.788ArgArg: 6.788 ± 0.683
4.811ArgSer: 4.811 ± 0.646
3.265ArgThr: 3.265 ± 0.552
4.554ArgVal: 4.554 ± 0.586
1.976ArgTrp: 1.976 ± 0.452
1.976ArgTyr: 1.976 ± 0.548
0.0ArgXaa: 0.0 ± 0.0
Ser
7.389SerAla: 7.389 ± 1.003
0.516SerCys: 0.516 ± 0.214
2.663SerAsp: 2.663 ± 0.462
1.804SerGlu: 1.804 ± 0.467
2.234SerPhe: 2.234 ± 0.475
6.788SerGly: 6.788 ± 0.894
1.203SerHis: 1.203 ± 0.318
2.749SerIle: 2.749 ± 0.54
1.632SerLys: 1.632 ± 0.361
3.694SerLeu: 3.694 ± 0.518
1.89SerMet: 1.89 ± 0.423
1.976SerAsn: 1.976 ± 0.403
2.921SerPro: 2.921 ± 0.535
2.234SerGln: 2.234 ± 0.474
3.866SerArg: 3.866 ± 0.731
2.578SerSer: 2.578 ± 0.722
3.609SerThr: 3.609 ± 1.362
3.093SerVal: 3.093 ± 0.613
0.601SerTrp: 0.601 ± 0.242
0.859SerTyr: 0.859 ± 0.253
0.0SerXaa: 0.0 ± 0.0
Thr
8.162ThrAla: 8.162 ± 0.74
0.344ThrCys: 0.344 ± 0.16
2.492ThrAsp: 2.492 ± 0.466
2.578ThrGlu: 2.578 ± 0.461
1.547ThrPhe: 1.547 ± 0.42
6.186ThrGly: 6.186 ± 1.364
0.859ThrHis: 0.859 ± 0.331
4.038ThrIle: 4.038 ± 1.083
1.461ThrLys: 1.461 ± 0.351
4.897ThrLeu: 4.897 ± 0.751
1.117ThrMet: 1.117 ± 0.323
1.031ThrAsn: 1.031 ± 0.291
4.725ThrPro: 4.725 ± 0.762
1.375ThrGln: 1.375 ± 0.349
3.952ThrArg: 3.952 ± 0.556
3.78ThrSer: 3.78 ± 1.422
2.234ThrThr: 2.234 ± 0.753
3.007ThrVal: 3.007 ± 0.525
0.773ThrTrp: 0.773 ± 0.25
0.945ThrTyr: 0.945 ± 0.267
0.0ThrXaa: 0.0 ± 0.0
Val
8.162ValAla: 8.162 ± 0.912
0.687ValCys: 0.687 ± 0.262
3.093ValAsp: 3.093 ± 0.54
3.694ValGlu: 3.694 ± 0.549
1.718ValPhe: 1.718 ± 0.358
3.351ValGly: 3.351 ± 0.54
1.031ValHis: 1.031 ± 0.283
3.093ValIle: 3.093 ± 0.581
2.234ValLys: 2.234 ± 0.584
4.124ValLeu: 4.124 ± 0.539
1.632ValMet: 1.632 ± 0.345
1.976ValAsn: 1.976 ± 0.487
3.093ValPro: 3.093 ± 0.579
3.866ValGln: 3.866 ± 0.517
4.983ValArg: 4.983 ± 0.725
4.124ValSer: 4.124 ± 0.591
4.554ValThr: 4.554 ± 0.725
3.437ValVal: 3.437 ± 0.479
1.632ValTrp: 1.632 ± 0.384
1.547ValTyr: 1.547 ± 0.313
0.0ValXaa: 0.0 ± 0.0
Trp
2.148TrpAla: 2.148 ± 0.369
0.172TrpCys: 0.172 ± 0.122
1.547TrpAsp: 1.547 ± 0.453
0.687TrpGlu: 0.687 ± 0.289
0.516TrpPhe: 0.516 ± 0.169
1.718TrpGly: 1.718 ± 0.432
0.43TrpHis: 0.43 ± 0.193
0.773TrpIle: 0.773 ± 0.232
0.601TrpLys: 0.601 ± 0.249
1.804TrpLeu: 1.804 ± 0.409
0.43TrpMet: 0.43 ± 0.201
1.117TrpAsn: 1.117 ± 0.36
0.516TrpPro: 0.516 ± 0.188
1.117TrpGln: 1.117 ± 0.399
1.718TrpArg: 1.718 ± 0.321
0.773TrpSer: 0.773 ± 0.292
1.547TrpThr: 1.547 ± 0.353
1.461TrpVal: 1.461 ± 0.372
0.601TrpTrp: 0.601 ± 0.278
0.43TrpTyr: 0.43 ± 0.258
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.749TyrAla: 2.749 ± 0.374
0.258TyrCys: 0.258 ± 0.135
1.289TyrAsp: 1.289 ± 0.355
0.43TyrGlu: 0.43 ± 0.165
0.43TyrPhe: 0.43 ± 0.195
2.148TyrGly: 2.148 ± 0.517
0.773TyrHis: 0.773 ± 0.221
0.43TyrIle: 0.43 ± 0.177
0.516TyrLys: 0.516 ± 0.208
1.203TyrLeu: 1.203 ± 0.337
0.344TyrMet: 0.344 ± 0.149
0.945TyrAsn: 0.945 ± 0.439
1.031TyrPro: 1.031 ± 0.263
0.773TyrGln: 0.773 ± 0.21
2.062TyrArg: 2.062 ± 0.453
0.859TyrSer: 0.859 ± 0.286
0.859TyrThr: 0.859 ± 0.245
1.632TyrVal: 1.632 ± 0.336
0.172TyrTrp: 0.172 ± 0.117
0.344TyrTyr: 0.344 ± 0.158
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (11640 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski