Vibrio phage VEN
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H5BN07|A0A2H5BN07_9CAUD DNA packaging protein OS=Vibrio phage VEN OX=2059879 PE=4 SV=1
MM1 pKa = 7.61 RR2 pKa = 11.84 VMSFEE7 pKa = 4.9 DD8 pKa = 3.44 WLDD11 pKa = 3.41 MYY13 pKa = 11.4 GDD15 pKa = 4.69 DD16 pKa = 4.21 VPSGIDD22 pKa = 3.27 LYY24 pKa = 10.82 EE25 pKa = 5.78 AYY27 pKa = 10.46 DD28 pKa = 3.85 SFVDD32 pKa = 3.56 SHH34 pKa = 8.12 KK35 pKa = 10.55 EE36 pKa = 3.89 QQLEE40 pKa = 4.11 TALLNDD46 pKa = 3.79
Molecular weight: 5.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.795
IPC2_protein 3.656
IPC_protein 3.567
Toseland 3.376
ProMoST 3.681
Dawson 3.592
Bjellqvist 3.834
Wikipedia 3.579
Rodwell 3.414
Grimsley 3.287
Solomon 3.554
Lehninger 3.503
Nozaki 3.732
DTASelect 3.948
Thurlkill 3.465
EMBOSS 3.579
Sillero 3.706
Patrickios 1.837
IPC_peptide 3.541
IPC2_peptide 3.668
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|A0A2H5BMY6|A0A2H5BMY6_9CAUD Uncharacterized protein OS=Vibrio phage VEN OX=2059879 PE=4 SV=1
MM1 pKa = 7.85 AIYY4 pKa = 10.38 SCGDD8 pKa = 2.77 ISTPSQSNEE17 pKa = 3.35 YY18 pKa = 9.2 RR19 pKa = 11.84 KK20 pKa = 10.82 AMLARR25 pKa = 11.84 QARR28 pKa = 11.84 EE29 pKa = 3.8 RR30 pKa = 11.84 HH31 pKa = 4.98 GKK33 pKa = 8.99 AAAFQLSNMHH43 pKa = 6.05 HH44 pKa = 5.64 TKK46 pKa = 10.61 KK47 pKa = 10.91 GDD49 pKa = 3.63 FTHH52 pKa = 7.16 LAPVGHH58 pKa = 7.23 LRR60 pKa = 11.84 TVIRR64 pKa = 11.84 GG65 pKa = 3.38
Molecular weight: 7.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 9.619
IPC_protein 10.101
Toseland 10.482
ProMoST 10.116
Dawson 10.613
Bjellqvist 10.277
Wikipedia 10.774
Rodwell 10.965
Grimsley 10.657
Solomon 10.687
Lehninger 10.657
Nozaki 10.467
DTASelect 10.277
Thurlkill 10.482
EMBOSS 10.877
Sillero 10.526
Patrickios 10.774
IPC_peptide 10.687
IPC2_peptide 9.18
IPC2.peptide.svr19 8.559
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
13660
41
1248
248.4
27.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.053 ± 0.482
1.018 ± 0.146
6.325 ± 0.201
7.255 ± 0.335
3.792 ± 0.208
7.306 ± 0.331
2.196 ± 0.218
5.337 ± 0.228
6.801 ± 0.353
8.272 ± 0.204
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.936 ± 0.17
4.693 ± 0.217
3.455 ± 0.226
4.012 ± 0.28
4.824 ± 0.239
6.186 ± 0.327
6.003 ± 0.316
6.589 ± 0.308
1.384 ± 0.147
3.565 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here