Amino acid dipepetide frequency for Vibrio phage VEN

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.712AlaAla: 8.712 ± 1.542
1.025AlaCys: 1.025 ± 0.324
5.637AlaAsp: 5.637 ± 0.648
5.784AlaGlu: 5.784 ± 0.617
3.441AlaPhe: 3.441 ± 0.399
4.686AlaGly: 4.686 ± 0.601
1.318AlaHis: 1.318 ± 0.313
4.978AlaIle: 4.978 ± 0.425
5.93AlaLys: 5.93 ± 0.665
5.711AlaLeu: 5.711 ± 0.723
2.709AlaMet: 2.709 ± 0.505
3.88AlaAsn: 3.88 ± 0.589
2.562AlaPro: 2.562 ± 0.415
3.587AlaGln: 3.587 ± 0.478
4.246AlaArg: 4.246 ± 0.708
4.027AlaSer: 4.027 ± 0.698
4.173AlaThr: 4.173 ± 0.58
4.027AlaVal: 4.027 ± 0.632
0.879AlaTrp: 0.879 ± 0.343
3.221AlaTyr: 3.221 ± 0.577
0.0AlaXaa: 0.0 ± 0.0
Cys
0.659CysAla: 0.659 ± 0.224
0.073CysCys: 0.073 ± 0.076
0.512CysAsp: 0.512 ± 0.182
0.659CysGlu: 0.659 ± 0.254
0.366CysPhe: 0.366 ± 0.183
0.659CysGly: 0.659 ± 0.229
0.073CysHis: 0.073 ± 0.063
0.586CysIle: 0.586 ± 0.233
1.391CysLys: 1.391 ± 0.343
0.879CysLeu: 0.879 ± 0.31
0.293CysMet: 0.293 ± 0.142
0.439CysAsn: 0.439 ± 0.16
0.512CysPro: 0.512 ± 0.219
0.366CysGln: 0.366 ± 0.161
0.586CysArg: 0.586 ± 0.223
0.659CysSer: 0.659 ± 0.263
0.293CysThr: 0.293 ± 0.133
0.586CysVal: 0.586 ± 0.231
0.22CysTrp: 0.22 ± 0.121
0.366CysTyr: 0.366 ± 0.164
0.0CysXaa: 0.0 ± 0.0
Asp
6.003AspAla: 6.003 ± 0.867
0.586AspCys: 0.586 ± 0.228
3.441AspAsp: 3.441 ± 0.433
4.393AspGlu: 4.393 ± 0.534
2.636AspPhe: 2.636 ± 0.445
4.978AspGly: 4.978 ± 0.54
1.025AspHis: 1.025 ± 0.335
4.466AspIle: 4.466 ± 0.471
4.686AspLys: 4.686 ± 0.74
4.686AspLeu: 4.686 ± 0.646
1.537AspMet: 1.537 ± 0.399
3.368AspAsn: 3.368 ± 0.535
1.391AspPro: 1.391 ± 0.305
1.611AspGln: 1.611 ± 0.274
2.05AspArg: 2.05 ± 0.345
4.1AspSer: 4.1 ± 0.61
4.686AspThr: 4.686 ± 0.671
4.539AspVal: 4.539 ± 0.548
1.171AspTrp: 1.171 ± 0.326
1.904AspTyr: 1.904 ± 0.299
0.0AspXaa: 0.0 ± 0.0
Glu
6.296GluAla: 6.296 ± 0.691
0.512GluCys: 0.512 ± 0.216
4.759GluAsp: 4.759 ± 0.567
6.443GluGlu: 6.443 ± 0.797
3.002GluPhe: 3.002 ± 0.375
5.491GluGly: 5.491 ± 0.591
1.977GluHis: 1.977 ± 0.366
2.343GluIle: 2.343 ± 0.722
5.052GluLys: 5.052 ± 0.9
6.223GluLeu: 6.223 ± 0.669
2.27GluMet: 2.27 ± 0.386
2.123GluAsn: 2.123 ± 0.458
2.343GluPro: 2.343 ± 0.485
2.782GluGln: 2.782 ± 0.404
3.587GluArg: 3.587 ± 0.624
4.173GluSer: 4.173 ± 0.749
3.661GluThr: 3.661 ± 0.723
5.564GluVal: 5.564 ± 0.607
1.611GluTrp: 1.611 ± 0.345
2.343GluTyr: 2.343 ± 0.39
0.0GluXaa: 0.0 ± 0.0
Phe
3.002PheAla: 3.002 ± 0.39
0.586PheCys: 0.586 ± 0.21
2.636PheAsp: 2.636 ± 0.412
2.782PheGlu: 2.782 ± 0.498
1.171PhePhe: 1.171 ± 0.285
2.27PheGly: 2.27 ± 0.447
0.659PheHis: 0.659 ± 0.214
2.27PheIle: 2.27 ± 0.462
2.416PheLys: 2.416 ± 0.417
2.709PheLeu: 2.709 ± 0.487
1.245PheMet: 1.245 ± 0.389
1.904PheAsn: 1.904 ± 0.362
1.611PhePro: 1.611 ± 0.359
0.879PheGln: 0.879 ± 0.269
1.977PheArg: 1.977 ± 0.31
2.855PheSer: 2.855 ± 0.419
2.636PheThr: 2.636 ± 0.516
2.416PheVal: 2.416 ± 0.452
0.512PheTrp: 0.512 ± 0.163
1.391PheTyr: 1.391 ± 0.394
0.0PheXaa: 0.0 ± 0.0
Gly
5.271GlyAla: 5.271 ± 0.716
1.025GlyCys: 1.025 ± 0.259
5.125GlyAsp: 5.125 ± 0.587
5.271GlyGlu: 5.271 ± 0.731
1.757GlyPhe: 1.757 ± 0.411
5.491GlyGly: 5.491 ± 0.716
1.83GlyHis: 1.83 ± 0.483
4.686GlyIle: 4.686 ± 0.53
5.857GlyLys: 5.857 ± 0.725
5.564GlyLeu: 5.564 ± 0.741
2.123GlyMet: 2.123 ± 0.461
3.514GlyAsn: 3.514 ± 0.441
0.732GlyPro: 0.732 ± 0.217
2.709GlyGln: 2.709 ± 0.392
3.148GlyArg: 3.148 ± 0.517
5.271GlySer: 5.271 ± 0.704
4.319GlyThr: 4.319 ± 0.685
4.686GlyVal: 4.686 ± 0.546
1.245GlyTrp: 1.245 ± 0.329
3.441GlyTyr: 3.441 ± 0.564
0.0GlyXaa: 0.0 ± 0.0
His
1.025HisAla: 1.025 ± 0.239
0.22HisCys: 0.22 ± 0.131
1.318HisAsp: 1.318 ± 0.334
1.025HisGlu: 1.025 ± 0.263
1.025HisPhe: 1.025 ± 0.27
1.83HisGly: 1.83 ± 0.319
0.659HisHis: 0.659 ± 0.234
1.098HisIle: 1.098 ± 0.28
2.27HisLys: 2.27 ± 0.49
2.416HisLeu: 2.416 ± 0.485
0.512HisMet: 0.512 ± 0.234
1.245HisAsn: 1.245 ± 0.297
0.805HisPro: 0.805 ± 0.249
0.732HisGln: 0.732 ± 0.255
0.952HisArg: 0.952 ± 0.276
1.245HisSer: 1.245 ± 0.324
0.952HisThr: 0.952 ± 0.271
1.391HisVal: 1.391 ± 0.294
0.366HisTrp: 0.366 ± 0.159
0.879HisTyr: 0.879 ± 0.277
0.0HisXaa: 0.0 ± 0.0
Ile
3.807IleAla: 3.807 ± 0.46
0.366IleCys: 0.366 ± 0.167
3.88IleAsp: 3.88 ± 0.512
4.612IleGlu: 4.612 ± 0.611
1.611IlePhe: 1.611 ± 0.344
3.148IleGly: 3.148 ± 0.622
1.318IleHis: 1.318 ± 0.33
2.709IleIle: 2.709 ± 0.393
4.173IleLys: 4.173 ± 0.48
3.953IleLeu: 3.953 ± 0.58
1.391IleMet: 1.391 ± 0.368
3.075IleAsn: 3.075 ± 0.659
2.05IlePro: 2.05 ± 0.368
2.636IleGln: 2.636 ± 0.365
2.636IleArg: 2.636 ± 0.48
4.173IleSer: 4.173 ± 0.748
3.075IleThr: 3.075 ± 0.511
2.855IleVal: 2.855 ± 0.534
0.366IleTrp: 0.366 ± 0.18
1.537IleTyr: 1.537 ± 0.353
0.0IleXaa: 0.0 ± 0.0
Lys
5.93LysAla: 5.93 ± 0.883
0.732LysCys: 0.732 ± 0.26
4.393LysAsp: 4.393 ± 0.808
5.784LysGlu: 5.784 ± 0.831
2.05LysPhe: 2.05 ± 0.478
5.711LysGly: 5.711 ± 0.797
2.05LysHis: 2.05 ± 0.437
2.196LysIle: 2.196 ± 0.41
3.661LysLys: 3.661 ± 0.515
5.198LysLeu: 5.198 ± 0.675
1.757LysMet: 1.757 ± 0.373
2.709LysAsn: 2.709 ± 0.347
2.855LysPro: 2.855 ± 0.537
3.002LysGln: 3.002 ± 0.543
4.686LysArg: 4.686 ± 0.629
3.734LysSer: 3.734 ± 0.582
3.002LysThr: 3.002 ± 0.52
4.612LysVal: 4.612 ± 0.553
0.879LysTrp: 0.879 ± 0.21
3.075LysTyr: 3.075 ± 0.433
0.0LysXaa: 0.0 ± 0.0
Leu
6.955LeuAla: 6.955 ± 0.765
0.805LeuCys: 0.805 ± 0.249
6.003LeuAsp: 6.003 ± 0.603
5.637LeuGlu: 5.637 ± 0.552
2.489LeuPhe: 2.489 ± 0.4
5.564LeuGly: 5.564 ± 0.651
1.977LeuHis: 1.977 ± 0.416
3.734LeuIle: 3.734 ± 0.51
4.539LeuLys: 4.539 ± 0.518
6.735LeuLeu: 6.735 ± 0.781
1.904LeuMet: 1.904 ± 0.381
3.807LeuAsn: 3.807 ± 0.545
3.807LeuPro: 3.807 ± 0.477
3.661LeuGln: 3.661 ± 0.654
5.125LeuArg: 5.125 ± 0.651
5.271LeuSer: 5.271 ± 0.554
5.711LeuThr: 5.711 ± 0.673
5.784LeuVal: 5.784 ± 0.708
0.879LeuTrp: 0.879 ± 0.217
2.343LeuTyr: 2.343 ± 0.459
0.0LeuXaa: 0.0 ± 0.0
Met
3.368MetAla: 3.368 ± 0.444
0.22MetCys: 0.22 ± 0.125
1.245MetAsp: 1.245 ± 0.372
1.977MetGlu: 1.977 ± 0.551
0.952MetPhe: 0.952 ± 0.274
1.757MetGly: 1.757 ± 0.335
0.512MetHis: 0.512 ± 0.17
1.464MetIle: 1.464 ± 0.281
2.05MetLys: 2.05 ± 0.401
3.514MetLeu: 3.514 ± 0.471
0.659MetMet: 0.659 ± 0.22
1.245MetAsn: 1.245 ± 0.26
1.318MetPro: 1.318 ± 0.282
1.171MetGln: 1.171 ± 0.341
1.318MetArg: 1.318 ± 0.34
2.123MetSer: 2.123 ± 0.406
1.83MetThr: 1.83 ± 0.371
1.245MetVal: 1.245 ± 0.259
0.146MetTrp: 0.146 ± 0.146
1.245MetTyr: 1.245 ± 0.291
0.0MetXaa: 0.0 ± 0.0
Asn
3.148AsnAla: 3.148 ± 0.389
0.659AsnCys: 0.659 ± 0.216
2.123AsnAsp: 2.123 ± 0.393
1.904AsnGlu: 1.904 ± 0.403
1.318AsnPhe: 1.318 ± 0.243
3.953AsnGly: 3.953 ± 0.59
0.805AsnHis: 0.805 ± 0.227
2.343AsnIle: 2.343 ± 0.32
4.466AsnLys: 4.466 ± 0.544
5.052AsnLeu: 5.052 ± 0.642
1.245AsnMet: 1.245 ± 0.262
2.782AsnAsn: 2.782 ± 0.543
2.123AsnPro: 2.123 ± 0.464
1.977AsnGln: 1.977 ± 0.342
2.343AsnArg: 2.343 ± 0.373
2.05AsnSer: 2.05 ± 0.425
3.368AsnThr: 3.368 ± 0.43
3.368AsnVal: 3.368 ± 0.585
0.22AsnTrp: 0.22 ± 0.131
1.684AsnTyr: 1.684 ± 0.452
0.0AsnXaa: 0.0 ± 0.0
Pro
2.27ProAla: 2.27 ± 0.381
0.366ProCys: 0.366 ± 0.143
3.075ProAsp: 3.075 ± 0.509
3.441ProGlu: 3.441 ± 0.527
1.611ProPhe: 1.611 ± 0.39
0.439ProGly: 0.439 ± 0.188
0.952ProHis: 0.952 ± 0.234
1.904ProIle: 1.904 ± 0.411
1.977ProLys: 1.977 ± 0.482
2.343ProLeu: 2.343 ± 0.352
1.098ProMet: 1.098 ± 0.306
1.611ProAsn: 1.611 ± 0.229
1.245ProPro: 1.245 ± 0.341
1.391ProGln: 1.391 ± 0.258
1.391ProArg: 1.391 ± 0.234
2.709ProSer: 2.709 ± 0.603
2.05ProThr: 2.05 ± 0.37
3.002ProVal: 3.002 ± 0.481
0.439ProTrp: 0.439 ± 0.139
1.245ProTyr: 1.245 ± 0.293
0.0ProXaa: 0.0 ± 0.0
Gln
3.441GlnAla: 3.441 ± 0.563
0.366GlnCys: 0.366 ± 0.156
1.83GlnAsp: 1.83 ± 0.314
4.173GlnGlu: 4.173 ± 0.649
1.684GlnPhe: 1.684 ± 0.51
2.855GlnGly: 2.855 ± 0.496
0.879GlnHis: 0.879 ± 0.23
2.343GlnIle: 2.343 ± 0.388
2.05GlnLys: 2.05 ± 0.471
3.734GlnLeu: 3.734 ± 0.561
1.245GlnMet: 1.245 ± 0.249
1.464GlnAsn: 1.464 ± 0.351
1.318GlnPro: 1.318 ± 0.332
1.537GlnGln: 1.537 ± 0.412
2.27GlnArg: 2.27 ± 0.346
2.416GlnSer: 2.416 ± 0.576
1.684GlnThr: 1.684 ± 0.435
3.148GlnVal: 3.148 ± 0.603
0.366GlnTrp: 0.366 ± 0.163
1.318GlnTyr: 1.318 ± 0.33
0.0GlnXaa: 0.0 ± 0.0
Arg
4.466ArgAla: 4.466 ± 0.936
0.732ArgCys: 0.732 ± 0.246
2.855ArgAsp: 2.855 ± 0.435
3.368ArgGlu: 3.368 ± 0.493
1.977ArgPhe: 1.977 ± 0.358
3.514ArgGly: 3.514 ± 0.474
0.659ArgHis: 0.659 ± 0.226
2.416ArgIle: 2.416 ± 0.462
2.489ArgLys: 2.489 ± 0.446
3.88ArgLeu: 3.88 ± 0.407
2.05ArgMet: 2.05 ± 0.428
1.83ArgAsn: 1.83 ± 0.388
1.391ArgPro: 1.391 ± 0.267
1.904ArgGln: 1.904 ± 0.345
2.416ArgArg: 2.416 ± 0.336
2.928ArgSer: 2.928 ± 0.451
2.562ArgThr: 2.562 ± 0.45
3.368ArgVal: 3.368 ± 0.503
1.098ArgTrp: 1.098 ± 0.256
2.343ArgTyr: 2.343 ± 0.386
0.0ArgXaa: 0.0 ± 0.0
Ser
3.661SerAla: 3.661 ± 0.644
0.659SerCys: 0.659 ± 0.207
3.88SerAsp: 3.88 ± 0.418
3.368SerGlu: 3.368 ± 0.565
2.782SerPhe: 2.782 ± 0.475
5.784SerGly: 5.784 ± 0.665
1.171SerHis: 1.171 ± 0.366
4.246SerIle: 4.246 ± 0.505
3.368SerLys: 3.368 ± 0.509
5.711SerLeu: 5.711 ± 0.577
2.196SerMet: 2.196 ± 0.563
3.148SerAsn: 3.148 ± 0.438
2.416SerPro: 2.416 ± 0.498
3.075SerGln: 3.075 ± 0.552
1.977SerArg: 1.977 ± 0.424
3.221SerSer: 3.221 ± 0.682
3.953SerThr: 3.953 ± 0.61
4.319SerVal: 4.319 ± 0.464
0.732SerTrp: 0.732 ± 0.26
2.196SerTyr: 2.196 ± 0.314
0.0SerXaa: 0.0 ± 0.0
Thr
3.807ThrAla: 3.807 ± 0.656
0.439ThrCys: 0.439 ± 0.179
3.807ThrAsp: 3.807 ± 0.647
3.88ThrGlu: 3.88 ± 0.547
3.441ThrPhe: 3.441 ± 0.632
5.637ThrGly: 5.637 ± 0.69
1.537ThrHis: 1.537 ± 0.299
3.368ThrIle: 3.368 ± 0.531
4.466ThrLys: 4.466 ± 0.616
5.125ThrLeu: 5.125 ± 0.686
1.757ThrMet: 1.757 ± 0.371
2.562ThrAsn: 2.562 ± 0.369
2.489ThrPro: 2.489 ± 0.412
2.343ThrGln: 2.343 ± 0.411
2.05ThrArg: 2.05 ± 0.37
3.734ThrSer: 3.734 ± 0.531
4.173ThrThr: 4.173 ± 0.695
3.514ThrVal: 3.514 ± 0.475
0.586ThrTrp: 0.586 ± 0.197
1.318ThrTyr: 1.318 ± 0.253
0.0ThrXaa: 0.0 ± 0.0
Val
4.759ValAla: 4.759 ± 0.439
0.366ValCys: 0.366 ± 0.16
3.661ValAsp: 3.661 ± 0.598
4.686ValGlu: 4.686 ± 0.718
2.416ValPhe: 2.416 ± 0.454
5.857ValGly: 5.857 ± 0.679
1.391ValHis: 1.391 ± 0.357
3.441ValIle: 3.441 ± 0.629
3.953ValLys: 3.953 ± 0.581
4.832ValLeu: 4.832 ± 0.54
2.123ValMet: 2.123 ± 0.371
3.441ValAsn: 3.441 ± 0.408
2.123ValPro: 2.123 ± 0.369
2.562ValGln: 2.562 ± 0.502
3.075ValArg: 3.075 ± 0.444
4.393ValSer: 4.393 ± 0.668
4.978ValThr: 4.978 ± 0.74
4.832ValVal: 4.832 ± 0.636
1.171ValTrp: 1.171 ± 0.333
1.83ValTyr: 1.83 ± 0.329
0.0ValXaa: 0.0 ± 0.0
Trp
0.952TrpAla: 0.952 ± 0.25
0.22TrpCys: 0.22 ± 0.126
0.805TrpAsp: 0.805 ± 0.252
1.245TrpGlu: 1.245 ± 0.314
0.512TrpPhe: 0.512 ± 0.165
0.659TrpGly: 0.659 ± 0.188
0.366TrpHis: 0.366 ± 0.147
0.439TrpIle: 0.439 ± 0.155
1.098TrpLys: 1.098 ± 0.41
1.245TrpLeu: 1.245 ± 0.333
0.512TrpMet: 0.512 ± 0.204
0.586TrpAsn: 0.586 ± 0.182
0.439TrpPro: 0.439 ± 0.185
0.366TrpGln: 0.366 ± 0.183
0.512TrpArg: 0.512 ± 0.165
0.805TrpSer: 0.805 ± 0.253
1.025TrpThr: 1.025 ± 0.285
1.245TrpVal: 1.245 ± 0.257
0.146TrpTrp: 0.146 ± 0.116
0.659TrpTyr: 0.659 ± 0.167
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.002TyrAla: 3.002 ± 0.309
0.22TyrCys: 0.22 ± 0.139
2.27TyrAsp: 2.27 ± 0.43
2.123TyrGlu: 2.123 ± 0.436
1.684TyrPhe: 1.684 ± 0.456
2.782TyrGly: 2.782 ± 0.498
0.805TyrHis: 0.805 ± 0.219
2.343TyrIle: 2.343 ± 0.378
2.123TyrLys: 2.123 ± 0.442
3.221TyrLeu: 3.221 ± 0.385
0.659TyrMet: 0.659 ± 0.196
2.196TyrAsn: 2.196 ± 0.5
1.025TyrPro: 1.025 ± 0.365
1.83TyrGln: 1.83 ± 0.335
1.904TyrArg: 1.904 ± 0.323
1.977TyrSer: 1.977 ± 0.386
1.904TyrThr: 1.904 ± 0.402
1.391TyrVal: 1.391 ± 0.245
0.805TyrTrp: 0.805 ± 0.213
1.318TyrTyr: 1.318 ± 0.294
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (13660 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski