Hericium alpestre

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Russulales; Hericiaceae; Hericium

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11000 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Z0AB03|A0A4Z0AB03_9AGAM CID domain-containing protein OS=Hericium alpestre OX=135208 GN=EWM64_g256 PE=4 SV=1
MM1 pKa = 7.58IFGIVYY7 pKa = 9.68DD8 pKa = 4.04AEE10 pKa = 4.3SVRR13 pKa = 11.84IVAHH17 pKa = 5.75VPYY20 pKa = 9.86EE21 pKa = 4.09IPDD24 pKa = 3.36EE25 pKa = 4.77DD26 pKa = 4.02GQAKK30 pKa = 9.24CQYY33 pKa = 9.46PRR35 pKa = 11.84YY36 pKa = 9.98SYY38 pKa = 9.22MSCVIDD44 pKa = 3.94TISYY48 pKa = 9.92RR49 pKa = 11.84GGRR52 pKa = 11.84RR53 pKa = 11.84CDD55 pKa = 3.04KK56 pKa = 10.7TSALDD61 pKa = 3.48RR62 pKa = 11.84FRR64 pKa = 11.84VAMAMVCLGIHH75 pKa = 6.55AEE77 pKa = 4.14FLAALLDD84 pKa = 4.62PSTSWPEE91 pKa = 3.96DD92 pKa = 3.29VVDD95 pKa = 3.64AAEE98 pKa = 4.35SVRR101 pKa = 11.84LLYY104 pKa = 10.59QPTLPTTSDD113 pKa = 3.39EE114 pKa = 4.3DD115 pKa = 4.05ASDD118 pKa = 4.61YY119 pKa = 11.53EE120 pKa = 4.65DD121 pKa = 4.14TPDD124 pKa = 5.47DD125 pKa = 3.74NTFDD129 pKa = 6.07DD130 pKa = 4.5EE131 pKa = 4.81DD132 pKa = 3.9TDD134 pKa = 3.82EE135 pKa = 6.1DD136 pKa = 4.0EE137 pKa = 4.95EE138 pKa = 4.48EE139 pKa = 4.39VAGEE143 pKa = 4.23CRR145 pKa = 3.56

Molecular weight: 16.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y9ZLW2|A0A4Y9ZLW2_9AGAM Tr-type G domain-containing protein OS=Hericium alpestre OX=135208 GN=EWM64_g8924 PE=4 SV=1
MM1 pKa = 7.2TVTVPRR7 pKa = 11.84TRR9 pKa = 11.84MHH11 pKa = 6.73RR12 pKa = 11.84SKK14 pKa = 9.44TLSLAAPRR22 pKa = 11.84APSFTTLPTEE32 pKa = 4.97LILDD36 pKa = 3.6ILEE39 pKa = 4.49RR40 pKa = 11.84ALLASPKK47 pKa = 9.37TGHH50 pKa = 6.78AYY52 pKa = 10.48ARR54 pKa = 11.84INKK57 pKa = 9.11HH58 pKa = 4.98IAHH61 pKa = 7.41LFDD64 pKa = 4.09QILYY68 pKa = 8.07RR69 pKa = 11.84TVVLASSRR77 pKa = 11.84QIALFARR84 pKa = 11.84TLASKK89 pKa = 10.35PPSFLPAHH97 pKa = 6.3VKK99 pKa = 10.29RR100 pKa = 11.84LAITWDD106 pKa = 3.58TPTTLPATTHH116 pKa = 5.56AHH118 pKa = 5.66LTTIIHH124 pKa = 6.49ACAGTRR130 pKa = 11.84ALAVPQEE137 pKa = 4.04LMSICLEE144 pKa = 4.2ACPPAHH150 pKa = 6.8HH151 pKa = 7.22PAAHH155 pKa = 7.52DD156 pKa = 3.89YY157 pKa = 7.39PAPMPADD164 pKa = 3.23ITIGAFTDD172 pKa = 3.66MAPLFPLRR180 pKa = 11.84TEE182 pKa = 4.12KK183 pKa = 10.49QALTSSTSAPPLFAGVTRR201 pKa = 11.84LRR203 pKa = 11.84IAEE206 pKa = 4.13PAGTWTAPRR215 pKa = 11.84AMLAQLGPLAQLTHH229 pKa = 6.26LHH231 pKa = 6.83LARR234 pKa = 11.84ARR236 pKa = 11.84ARR238 pKa = 11.84MRR240 pKa = 11.84TTTSRR245 pKa = 11.84SRR247 pKa = 11.84RR248 pKa = 11.84TSPDD252 pKa = 2.83CSGPGAGVDD261 pKa = 3.59PVKK264 pKa = 10.63EE265 pKa = 3.94IGSAEE270 pKa = 4.07LNHH273 pKa = 6.65

Molecular weight: 29.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11000

0

11000

4291909

20

3630

390.2

43.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.24 ± 0.022

1.196 ± 0.009

5.97 ± 0.017

6.19 ± 0.022

3.716 ± 0.014

6.385 ± 0.02

2.626 ± 0.01

4.755 ± 0.018

4.679 ± 0.026

9.189 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.213 ± 0.01

3.224 ± 0.013

6.41 ± 0.033

3.755 ± 0.014

6.092 ± 0.021

7.989 ± 0.031

5.743 ± 0.016

6.429 ± 0.018

1.437 ± 0.01

2.609 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski