Marinomonas mediterranea (strain ATCC 700492 / JCM 21426 / NBRC 103028 / MMB-1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Marinomonas; Marinomonas mediterranea

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4118 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F2JYP6|F2JYP6_MARM1 Uncharacterized protein OS=Marinomonas mediterranea (strain ATCC 700492 / JCM 21426 / NBRC 103028 / MMB-1) OX=717774 GN=Marme_0371 PE=4 SV=1
MM1 pKa = 7.06KK2 pKa = 9.82TVLKK6 pKa = 9.58YY7 pKa = 8.9TVQPGDD13 pKa = 4.14SLSKK17 pKa = 10.55IADD20 pKa = 3.73QISASAGITTDD31 pKa = 3.34QIEE34 pKa = 4.23AANPSVVPSALQIGQLLTIPQLDD57 pKa = 3.96TPTNRR62 pKa = 11.84WFYY65 pKa = 10.45TVLSGDD71 pKa = 3.9SFSGIAAALAQCKK84 pKa = 9.93GLTYY88 pKa = 10.9EE89 pKa = 5.51EE90 pKa = 4.75IEE92 pKa = 4.17QDD94 pKa = 3.3NSLTGSTIDD103 pKa = 3.25VGQVLNIPATSSDD116 pKa = 4.81APTQDD121 pKa = 3.19NLAPNAINMGYY132 pKa = 9.5WNWTWSGTSNPSNATLSLAFSGWTDD157 pKa = 3.36PTTALQDD164 pKa = 3.46SHH166 pKa = 6.18QVKK169 pKa = 10.07PSLVGTKK176 pKa = 10.22YY177 pKa = 9.97LTFGGGNDD185 pKa = 3.44NGKK188 pKa = 8.32FTALSLQDD196 pKa = 3.21ITSAIQSGKK205 pKa = 9.93LEE207 pKa = 4.43GYY209 pKa = 9.97EE210 pKa = 4.02GVAYY214 pKa = 10.03DD215 pKa = 3.75VEE217 pKa = 4.72EE218 pKa = 4.84GDD220 pKa = 3.79SHH222 pKa = 8.56LEE224 pKa = 3.47NDD226 pKa = 3.76FAVSFKK232 pKa = 10.48AAKK235 pKa = 10.02DD236 pKa = 3.29AGLKK240 pKa = 10.12VLVTVSHH247 pKa = 6.43SAPYY251 pKa = 10.65GITDD255 pKa = 4.41ADD257 pKa = 3.63ALMQSFFADD266 pKa = 3.56SNIDD270 pKa = 3.63LLSPQLYY277 pKa = 9.0TEE279 pKa = 4.37GDD281 pKa = 3.56EE282 pKa = 4.85TEE284 pKa = 4.65NDD286 pKa = 3.61YY287 pKa = 9.58QTTSGTSTTWEE298 pKa = 4.54DD299 pKa = 3.54YY300 pKa = 11.28ASAKK304 pKa = 9.88AAIVPSIVTSDD315 pKa = 4.33LYY317 pKa = 11.46DD318 pKa = 3.48SATGYY323 pKa = 8.13FTEE326 pKa = 4.95QGVTLAGYY334 pKa = 8.51IQWAQVV340 pKa = 2.84

Molecular weight: 36.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F2K1V7|F2K1V7_MARM1 MscS Mechanosensitive ion channel OS=Marinomonas mediterranea (strain ATCC 700492 / JCM 21426 / NBRC 103028 / MMB-1) OX=717774 GN=Marme_0667 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.41GGRR28 pKa = 11.84QVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.94ALSAA44 pKa = 4.07

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4118

0

4118

1345423

30

3387

326.7

36.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.417 ± 0.042

1.007 ± 0.012

5.674 ± 0.031

6.475 ± 0.033

4.151 ± 0.029

6.717 ± 0.032

2.197 ± 0.019

6.319 ± 0.034

5.323 ± 0.035

10.438 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.518 ± 0.018

4.175 ± 0.024

3.87 ± 0.021

4.097 ± 0.03

4.604 ± 0.031

7.349 ± 0.038

5.412 ± 0.037

7.024 ± 0.033

1.229 ± 0.013

3.003 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski