Thiocystis violascens (strain ATCC 17096 / DSM 198 / 6111) (Chromatium violascens)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Thiocystis; Thiocystis violascens

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4192 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3YB38|I3YB38_THIV6 Glycosyl transferase OS=Thiocystis violascens (strain ATCC 17096 / DSM 198 / 6111) OX=765911 GN=Thivi_2257 PE=4 SV=1
MM1 pKa = 7.82DD2 pKa = 5.89RR3 pKa = 11.84SDD5 pKa = 3.77TDD7 pKa = 3.11SDD9 pKa = 3.59TDD11 pKa = 3.54RR12 pKa = 11.84EE13 pKa = 4.28EE14 pKa = 4.38IEE16 pKa = 4.31PVPLATCICQRR27 pKa = 11.84SGFDD31 pKa = 3.04QQARR35 pKa = 11.84LDD37 pKa = 3.97NRR39 pKa = 11.84SSHH42 pKa = 6.95PDD44 pKa = 3.36CSDD47 pKa = 3.23ACCQQGWEE55 pKa = 4.25SSADD59 pKa = 3.72GACQSDD65 pKa = 3.62DD66 pKa = 4.01CEE68 pKa = 5.0FVAQCGGDD76 pKa = 3.21

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3Y5T9|I3Y5T9_THIV6 KaiB domain-containing protein OS=Thiocystis violascens (strain ATCC 17096 / DSM 198 / 6111) OX=765911 GN=Thivi_0288 PE=4 SV=1
MM1 pKa = 7.86DD2 pKa = 3.51VFLIVEE8 pKa = 4.34QILGLVWWLIPLALLVGLLKK28 pKa = 10.83SPSVKK33 pKa = 10.45GYY35 pKa = 10.1LGEE38 pKa = 4.15SRR40 pKa = 11.84VRR42 pKa = 11.84RR43 pKa = 11.84IARR46 pKa = 11.84TRR48 pKa = 11.84LDD50 pKa = 3.18PMTYY54 pKa = 10.16HH55 pKa = 7.42PLHH58 pKa = 6.63NLTLPTPDD66 pKa = 2.96GTTQIDD72 pKa = 3.74HH73 pKa = 6.06VFVSRR78 pKa = 11.84FGLFVLEE85 pKa = 4.47TKK87 pKa = 10.99NMTGWIFGGEE97 pKa = 4.16HH98 pKa = 4.89QAQWTQKK105 pKa = 9.09IYY107 pKa = 10.96RR108 pKa = 11.84RR109 pKa = 11.84TTRR112 pKa = 11.84FQNPLRR118 pKa = 11.84QNYY121 pKa = 8.6KK122 pKa = 9.71HH123 pKa = 6.42LKK125 pKa = 8.26ALEE128 pKa = 4.18RR129 pKa = 11.84ALPVPLEE136 pKa = 4.52TIHH139 pKa = 6.93SVVAFVGDD147 pKa = 3.86STFKK151 pKa = 10.85TEE153 pKa = 3.95MPPNVTQGNGFVRR166 pKa = 11.84HH167 pKa = 5.3IQSFRR172 pKa = 11.84EE173 pKa = 3.94PVFTEE178 pKa = 4.09TQVVEE183 pKa = 3.95LVRR186 pKa = 11.84RR187 pKa = 11.84LQDD190 pKa = 2.96GRR192 pKa = 11.84LAPTLATHH200 pKa = 6.91RR201 pKa = 11.84AHH203 pKa = 6.81VRR205 pKa = 11.84SLKK208 pKa = 10.03QRR210 pKa = 11.84SNPDD214 pKa = 2.69ASQRR218 pKa = 11.84CPRR221 pKa = 11.84CGRR224 pKa = 11.84PMILRR229 pKa = 11.84TVKK232 pKa = 10.39KK233 pKa = 8.49GTQAGRR239 pKa = 11.84RR240 pKa = 11.84FWACSGFPDD249 pKa = 4.29CQSTRR254 pKa = 11.84DD255 pKa = 3.68LPP257 pKa = 4.04

Molecular weight: 29.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4192

0

4192

1375469

26

2896

328.1

36.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.428 ± 0.047

1.003 ± 0.013

6.006 ± 0.031

6.148 ± 0.037

3.486 ± 0.02

7.925 ± 0.035

2.229 ± 0.019

4.987 ± 0.028

2.733 ± 0.029

11.489 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.191 ± 0.018

2.553 ± 0.023

5.312 ± 0.032

3.717 ± 0.027

7.969 ± 0.044

5.242 ± 0.027

5.117 ± 0.033

6.77 ± 0.033

1.404 ± 0.017

2.289 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski