Viridibacillus arenosi FSL R5-213

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Viridibacillus; Viridibacillus arenosi

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4296 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W4EWF0|W4EWF0_9BACL Putative Ply protein OS=Viridibacillus arenosi FSL R5-213 OX=1227360 GN=C176_12373 PE=4 SV=1
MM1 pKa = 7.93DD2 pKa = 5.37PEE4 pKa = 4.91KK5 pKa = 10.65LICDD9 pKa = 4.02SCCEE13 pKa = 4.03NNQVLWPRR21 pKa = 11.84IKK23 pKa = 10.75AIDD26 pKa = 3.72IGSIQPPPVVIPEE39 pKa = 4.06GTIIPIINRR48 pKa = 11.84YY49 pKa = 8.68FFIVTEE55 pKa = 5.42DD56 pKa = 3.13IDD58 pKa = 4.1LTNGATIPANLFTDD72 pKa = 4.32DD73 pKa = 4.17NNNPLTEE80 pKa = 4.47FKK82 pKa = 10.87LFNPNGYY89 pKa = 10.2VNLYY93 pKa = 9.48INAVMQEE100 pKa = 3.56GGAYY104 pKa = 9.44KK105 pKa = 9.07VTPDD109 pKa = 4.11PLIINPFNGTIFAGTPIIIEE129 pKa = 4.14SLGFQQMM136 pKa = 3.36

Molecular weight: 15.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W4F6W5|W4F6W5_9BACL Nicotinate phosphoribosyltransferase OS=Viridibacillus arenosi FSL R5-213 OX=1227360 GN=C176_02019 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.54VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 9.98NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4296

0

4296

1217398

22

2008

283.4

31.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.02 ± 0.043

0.7 ± 0.01

5.045 ± 0.029

7.257 ± 0.039

4.616 ± 0.029

6.592 ± 0.038

1.964 ± 0.017

8.316 ± 0.041

7.099 ± 0.034

9.754 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.796 ± 0.021

4.626 ± 0.032

3.38 ± 0.018

3.784 ± 0.025

3.688 ± 0.028

6.14 ± 0.029

5.681 ± 0.03

7.022 ± 0.033

0.99 ± 0.011

3.529 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski