Amino acid dipepetide frequency for Viridibacillus arenosi FSL R5-213

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.279AlaAla: 5.279 ± 0.083	0.545AlaCys: 0.545 ± 0.023	3.481AlaAsp: 3.481 ± 0.056	4.664AlaGlu: 4.664 ± 0.075	3.411AlaPhe: 3.411 ± 0.059	4.961AlaGly: 4.961 ± 0.091	1.235AlaHis: 1.235 ± 0.033	6.584AlaIle: 6.584 ± 0.089	4.98AlaLys: 4.98 ± 0.066	7.127AlaLeu: 7.127 ± 0.087	2.041AlaMet: 2.041 ± 0.038	3.027AlaAsn: 3.027 ± 0.055	1.953AlaPro: 1.953 ± 0.048	2.284AlaGln: 2.284 ± 0.046	2.379AlaArg: 2.379 ± 0.044	4.225AlaSer: 4.225 ± 0.066	4.029AlaThr: 4.029 ± 0.071	5.136AlaVal: 5.136 ± 0.075	0.536AlaTrp: 0.536 ± 0.021	2.326AlaTyr: 2.326 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	0.425CysAla: 0.425 ± 0.019	0.08CysCys: 0.08 ± 0.009	0.355CysAsp: 0.355 ± 0.016	0.478CysGlu: 0.478 ± 0.022	0.301CysPhe: 0.301 ± 0.015	0.636CysGly: 0.636 ± 0.023	0.188CysHis: 0.188 ± 0.011	0.618CysIle: 0.618 ± 0.028	0.346CysLys: 0.346 ± 0.019	0.623CysLeu: 0.623 ± 0.027	0.188CysMet: 0.188 ± 0.013	0.31CysAsn: 0.31 ± 0.014	0.309CysPro: 0.309 ± 0.017	0.238CysGln: 0.238 ± 0.016	0.279CysArg: 0.279 ± 0.017	0.493CysSer: 0.493 ± 0.022	0.397CysThr: 0.397 ± 0.018	0.407CysVal: 0.407 ± 0.017	0.08CysTrp: 0.08 ± 0.009	0.252CysTyr: 0.252 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.456AspAla: 3.456 ± 0.061	0.361AspCys: 0.361 ± 0.018	2.482AspAsp: 2.482 ± 0.048	4.425AspGlu: 4.425 ± 0.068	2.505AspPhe: 2.505 ± 0.045	3.364AspGly: 3.364 ± 0.066	1.01AspHis: 1.01 ± 0.029	4.279AspIle: 4.279 ± 0.068	3.285AspLys: 3.285 ± 0.063	4.841AspLeu: 4.841 ± 0.068	1.41AspMet: 1.41 ± 0.038	1.978AspAsn: 1.978 ± 0.047	1.688AspPro: 1.688 ± 0.036	1.806AspGln: 1.806 ± 0.036	1.942AspArg: 1.942 ± 0.044	2.654AspSer: 2.654 ± 0.048	2.474AspThr: 2.474 ± 0.049	3.786AspVal: 3.786 ± 0.058	0.574AspTrp: 0.574 ± 0.02	2.13AspTyr: 2.13 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	5.051GluAla: 5.051 ± 0.078	0.389GluCys: 0.389 ± 0.02	3.671GluAsp: 3.671 ± 0.056	6.36GluGlu: 6.36 ± 0.101	2.609GluPhe: 2.609 ± 0.043	4.144GluGly: 4.144 ± 0.069	1.444GluHis: 1.444 ± 0.038	5.711GluIle: 5.711 ± 0.077	6.263GluLys: 6.263 ± 0.084	7.191GluLeu: 7.191 ± 0.083	2.18GluMet: 2.18 ± 0.042	3.705GluAsn: 3.705 ± 0.057	1.921GluPro: 1.921 ± 0.037	3.591GluGln: 3.591 ± 0.069	3.128GluArg: 3.128 ± 0.055	3.569GluSer: 3.569 ± 0.059	3.566GluThr: 3.566 ± 0.053	5.048GluVal: 5.048 ± 0.071	0.831GluTrp: 0.831 ± 0.027	2.199GluTyr: 2.199 ± 0.05	0.0GluXaa: 0.0 ± 0.0
Phe	3.189PheAla: 3.189 ± 0.049	0.366PheCys: 0.366 ± 0.016	2.375PheAsp: 2.375 ± 0.042	3.166PheGlu: 3.166 ± 0.058	2.353PhePhe: 2.353 ± 0.054	3.337PheGly: 3.337 ± 0.06	0.882PheHis: 0.882 ± 0.024	4.15PheIle: 4.15 ± 0.069	2.738PheLys: 2.738 ± 0.049	4.386PheLeu: 4.386 ± 0.074	1.232PheMet: 1.232 ± 0.033	2.074PheAsn: 2.074 ± 0.039	1.557PhePro: 1.557 ± 0.037	1.575PheGln: 1.575 ± 0.038	1.384PheArg: 1.384 ± 0.033	3.235PheSer: 3.235 ± 0.054	2.734PheThr: 2.734 ± 0.044	3.282PheVal: 3.282 ± 0.059	0.468PheTrp: 0.468 ± 0.022	1.683PheTyr: 1.683 ± 0.044	0.0PheXaa: 0.0 ± 0.0
Gly	4.676GlyAla: 4.676 ± 0.079	0.565GlyCys: 0.565 ± 0.023	3.093GlyAsp: 3.093 ± 0.06	4.303GlyGlu: 4.303 ± 0.072	3.324GlyPhe: 3.324 ± 0.055	4.407GlyGly: 4.407 ± 0.082	1.328GlyHis: 1.328 ± 0.037	5.872GlyIle: 5.872 ± 0.081	4.872GlyLys: 4.872 ± 0.07	6.364GlyLeu: 6.364 ± 0.089	1.993GlyMet: 1.993 ± 0.044	2.725GlyAsn: 2.725 ± 0.049	1.562GlyPro: 1.562 ± 0.038	2.218GlyGln: 2.218 ± 0.048	2.404GlyArg: 2.404 ± 0.047	3.923GlySer: 3.923 ± 0.061	3.963GlyThr: 3.963 ± 0.068	4.886GlyVal: 4.886 ± 0.071	0.747GlyTrp: 0.747 ± 0.025	2.69GlyTyr: 2.69 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	1.277HisAla: 1.277 ± 0.035	0.186HisCys: 0.186 ± 0.013	0.976HisAsp: 0.976 ± 0.029	1.373HisGlu: 1.373 ± 0.037	1.055HisPhe: 1.055 ± 0.029	1.262HisGly: 1.262 ± 0.031	0.581HisHis: 0.581 ± 0.023	1.587HisIle: 1.587 ± 0.039	1.034HisLys: 1.034 ± 0.029	1.976HisLeu: 1.976 ± 0.042	0.512HisMet: 0.512 ± 0.023	0.821HisAsn: 0.821 ± 0.028	0.996HisPro: 0.996 ± 0.034	0.791HisGln: 0.791 ± 0.023	0.707HisArg: 0.707 ± 0.023	1.133HisSer: 1.133 ± 0.029	1.034HisThr: 1.034 ± 0.029	1.313HisVal: 1.313 ± 0.031	0.204HisTrp: 0.204 ± 0.015	0.825HisTyr: 0.825 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	6.576IleAla: 6.576 ± 0.088	0.665IleCys: 0.665 ± 0.025	4.547IleAsp: 4.547 ± 0.059	6.078IleGlu: 6.078 ± 0.083	3.587IlePhe: 3.587 ± 0.066	6.111IleGly: 6.111 ± 0.091	1.617IleHis: 1.617 ± 0.042	6.842IleIle: 6.842 ± 0.1	4.687IleLys: 4.687 ± 0.069	7.837IleLeu: 7.837 ± 0.096	1.942IleMet: 1.942 ± 0.043	3.469IleAsn: 3.469 ± 0.06	3.26IlePro: 3.26 ± 0.059	3.202IleGln: 3.202 ± 0.057	3.134IleArg: 3.134 ± 0.056	5.477IleSer: 5.477 ± 0.064	4.707IleThr: 4.707 ± 0.064	6.189IleVal: 6.189 ± 0.086	0.708IleTrp: 0.708 ± 0.024	2.531IleTyr: 2.531 ± 0.051	0.0IleXaa: 0.0 ± 0.0
Lys	4.713LysAla: 4.713 ± 0.066	0.356LysCys: 0.356 ± 0.017	3.84LysAsp: 3.84 ± 0.08	6.585LysGlu: 6.585 ± 0.079	2.172LysPhe: 2.172 ± 0.041	4.393LysGly: 4.393 ± 0.065	1.253LysHis: 1.253 ± 0.033	5.052LysIle: 5.052 ± 0.066	6.145LysLys: 6.145 ± 0.091	6.254LysLeu: 6.254 ± 0.077	2.459LysMet: 2.459 ± 0.049	3.591LysAsn: 3.591 ± 0.055	2.173LysPro: 2.173 ± 0.049	3.033LysGln: 3.033 ± 0.067	2.998LysArg: 2.998 ± 0.054	3.958LysSer: 3.958 ± 0.061	3.856LysThr: 3.856 ± 0.06	4.926LysVal: 4.926 ± 0.062	0.867LysTrp: 0.867 ± 0.027	2.367LysTyr: 2.367 ± 0.05	0.0LysXaa: 0.0 ± 0.0
Leu	7.247LeuAla: 7.247 ± 0.089	0.689LeuCys: 0.689 ± 0.024	4.7LeuAsp: 4.7 ± 0.072	6.47LeuGlu: 6.47 ± 0.085	4.837LeuPhe: 4.837 ± 0.079	6.022LeuGly: 6.022 ± 0.079	1.909LeuHis: 1.909 ± 0.039	7.522LeuIle: 7.522 ± 0.107	6.787LeuLys: 6.787 ± 0.083	10.278LeuLeu: 10.278 ± 0.122	2.588LeuMet: 2.588 ± 0.042	4.516LeuAsn: 4.516 ± 0.059	3.76LeuPro: 3.76 ± 0.061	3.973LeuGln: 3.973 ± 0.077	3.449LeuArg: 3.449 ± 0.058	6.733LeuSer: 6.733 ± 0.082	5.735LeuThr: 5.735 ± 0.081	6.369LeuVal: 6.369 ± 0.082	0.812LeuTrp: 0.812 ± 0.03	3.148LeuTyr: 3.148 ± 0.052	0.0LeuXaa: 0.0 ± 0.0
Met	1.986MetAla: 1.986 ± 0.045	0.14MetCys: 0.14 ± 0.01	1.475MetAsp: 1.475 ± 0.037	1.744MetGlu: 1.744 ± 0.041	1.106MetPhe: 1.106 ± 0.031	1.711MetGly: 1.711 ± 0.042	0.508MetHis: 0.508 ± 0.021	2.15MetIle: 2.15 ± 0.045	2.625MetLys: 2.625 ± 0.05	2.569MetLeu: 2.569 ± 0.051	0.875MetMet: 0.875 ± 0.032	1.55MetAsn: 1.55 ± 0.039	1.035MetPro: 1.035 ± 0.035	1.12MetGln: 1.12 ± 0.033	1.124MetArg: 1.124 ± 0.028	1.746MetSer: 1.746 ± 0.032	1.89MetThr: 1.89 ± 0.041	1.588MetVal: 1.588 ± 0.036	0.194MetTrp: 0.194 ± 0.013	0.829MetTyr: 0.829 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	3.111AsnAla: 3.111 ± 0.047	0.325AsnCys: 0.325 ± 0.018	2.399AsnAsp: 2.399 ± 0.046	3.459AsnGlu: 3.459 ± 0.064	1.809AsnPhe: 1.809 ± 0.034	3.446AsnGly: 3.446 ± 0.061	0.987AsnHis: 0.987 ± 0.029	3.746AsnIle: 3.746 ± 0.069	3.213AsnLys: 3.213 ± 0.06	4.036AsnLeu: 4.036 ± 0.067	1.281AsnMet: 1.281 ± 0.031	2.325AsnAsn: 2.325 ± 0.051	2.031AsnPro: 2.031 ± 0.045	1.727AsnGln: 1.727 ± 0.038	1.938AsnArg: 1.938 ± 0.038	2.646AsnSer: 2.646 ± 0.056	2.523AsnThr: 2.523 ± 0.054	3.136AsnVal: 3.136 ± 0.053	0.486AsnTrp: 0.486 ± 0.021	1.64AsnTyr: 1.64 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	2.102ProAla: 2.102 ± 0.038	0.201ProCys: 0.201 ± 0.012	1.69ProAsp: 1.69 ± 0.039	2.564ProGlu: 2.564 ± 0.044	1.902ProPhe: 1.902 ± 0.046	1.997ProGly: 1.997 ± 0.048	0.715ProHis: 0.715 ± 0.025	3.075ProIle: 3.075 ± 0.054	2.223ProLys: 2.223 ± 0.04	3.234ProLeu: 3.234 ± 0.054	0.816ProMet: 0.816 ± 0.034	1.723ProAsn: 1.723 ± 0.041	0.858ProPro: 0.858 ± 0.033	1.065ProGln: 1.065 ± 0.031	0.977ProArg: 0.977 ± 0.029	2.206ProSer: 2.206 ± 0.045	2.173ProThr: 2.173 ± 0.045	2.618ProVal: 2.618 ± 0.048	0.304ProTrp: 0.304 ± 0.015	1.352ProTyr: 1.352 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	2.626GlnAla: 2.626 ± 0.054	0.199GlnCys: 0.199 ± 0.012	1.739GlnAsp: 1.739 ± 0.036	2.642GlnGlu: 2.642 ± 0.051	1.681GlnPhe: 1.681 ± 0.039	2.081GlnGly: 2.081 ± 0.052	0.825GlnHis: 0.825 ± 0.027	2.893GlnIle: 2.893 ± 0.049	2.793GlnLys: 2.793 ± 0.054	4.312GlnLeu: 4.312 ± 0.08	1.134GlnMet: 1.134 ± 0.032	1.75GlnAsn: 1.75 ± 0.04	1.224GlnPro: 1.224 ± 0.034	2.035GlnGln: 2.035 ± 0.057	1.39GlnArg: 1.39 ± 0.038	2.277GlnSer: 2.277 ± 0.044	1.97GlnThr: 1.97 ± 0.038	2.378GlnVal: 2.378 ± 0.045	0.428GlnTrp: 0.428 ± 0.022	1.459GlnTyr: 1.459 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	2.356ArgAla: 2.356 ± 0.045	0.239ArgCys: 0.239 ± 0.015	1.895ArgAsp: 1.895 ± 0.042	2.81ArgGlu: 2.81 ± 0.059	1.816ArgPhe: 1.816 ± 0.037	2.127ArgGly: 2.127 ± 0.047	0.76ArgHis: 0.76 ± 0.022	3.048ArgIle: 3.048 ± 0.052	2.946ArgLys: 2.946 ± 0.047	3.627ArgLeu: 3.627 ± 0.061	1.171ArgMet: 1.171 ± 0.034	1.725ArgAsn: 1.725 ± 0.036	1.166ArgPro: 1.166 ± 0.032	1.476ArgGln: 1.476 ± 0.042	1.523ArgArg: 1.523 ± 0.04	2.014ArgSer: 2.014 ± 0.039	1.95ArgThr: 1.95 ± 0.038	2.47ArgVal: 2.47 ± 0.05	0.342ArgTrp: 0.342 ± 0.017	1.418ArgTyr: 1.418 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	3.951SerAla: 3.951 ± 0.068	0.395SerCys: 0.395 ± 0.019	2.872SerAsp: 2.872 ± 0.053	3.88SerGlu: 3.88 ± 0.058	3.293SerPhe: 3.293 ± 0.06	4.196SerGly: 4.196 ± 0.064	1.12SerHis: 1.12 ± 0.031	5.652SerIle: 5.652 ± 0.066	4.379SerLys: 4.379 ± 0.059	6.074SerLeu: 6.074 ± 0.078	1.69SerMet: 1.69 ± 0.039	3.005SerAsn: 3.005 ± 0.056	2.032SerPro: 2.032 ± 0.047	1.937SerGln: 1.937 ± 0.041	1.997SerArg: 1.997 ± 0.04	4.128SerSer: 4.128 ± 0.071	3.602SerThr: 3.602 ± 0.063	4.251SerVal: 4.251 ± 0.056	0.601SerTrp: 0.601 ± 0.023	2.346SerTyr: 2.346 ± 0.045	0.0SerXaa: 0.0 ± 0.0
Thr	4.156ThrAla: 4.156 ± 0.059	0.341ThrCys: 0.341 ± 0.017	2.793ThrAsp: 2.793 ± 0.052	3.592ThrGlu: 3.592 ± 0.053	2.776ThrPhe: 2.776 ± 0.054	4.085ThrGly: 4.085 ± 0.068	1.075ThrHis: 1.075 ± 0.029	4.991ThrIle: 4.991 ± 0.074	3.802ThrLys: 3.802 ± 0.061	5.486ThrLeu: 5.486 ± 0.067	1.358ThrMet: 1.358 ± 0.035	2.721ThrAsn: 2.721 ± 0.054	2.209ThrPro: 2.209 ± 0.047	1.587ThrGln: 1.587 ± 0.042	1.752ThrArg: 1.752 ± 0.038	3.564ThrSer: 3.564 ± 0.064	3.539ThrThr: 3.539 ± 0.063	4.396ThrVal: 4.396 ± 0.065	0.55ThrTrp: 0.55 ± 0.022	2.035ThrTyr: 2.035 ± 0.049	0.0ThrXaa: 0.0 ± 0.0
Val	5.219ValAla: 5.219 ± 0.076	0.55ValCys: 0.55 ± 0.022	3.604ValAsp: 3.604 ± 0.067	4.894ValGlu: 4.894 ± 0.088	3.226ValPhe: 3.226 ± 0.051	4.633ValGly: 4.633 ± 0.076	1.286ValHis: 1.286 ± 0.035	5.738ValIle: 5.738 ± 0.083	4.878ValLys: 4.878 ± 0.071	6.805ValLeu: 6.805 ± 0.089	1.847ValMet: 1.847 ± 0.044	3.153ValAsn: 3.153 ± 0.052	2.539ValPro: 2.539 ± 0.046	2.482ValGln: 2.482 ± 0.047	2.511ValArg: 2.511 ± 0.047	4.538ValSer: 4.538 ± 0.068	4.229ValThr: 4.229 ± 0.068	5.243ValVal: 5.243 ± 0.08	0.609ValTrp: 0.609 ± 0.022	2.236ValTyr: 2.236 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	0.58TrpAla: 0.58 ± 0.024	0.077TrpCys: 0.077 ± 0.007	0.48TrpAsp: 0.48 ± 0.02	0.554TrpGlu: 0.554 ± 0.022	0.491TrpPhe: 0.491 ± 0.022	0.649TrpGly: 0.649 ± 0.022	0.204TrpHis: 0.204 ± 0.013	0.881TrpIle: 0.881 ± 0.031	0.664TrpLys: 0.664 ± 0.026	1.137TrpLeu: 1.137 ± 0.036	0.326TrpMet: 0.326 ± 0.019	0.489TrpAsn: 0.489 ± 0.022	0.253TrpPro: 0.253 ± 0.014	0.428TrpGln: 0.428 ± 0.022	0.413TrpArg: 0.413 ± 0.021	0.647TrpSer: 0.647 ± 0.022	0.528TrpThr: 0.528 ± 0.022	0.61TrpVal: 0.61 ± 0.023	0.13TrpTrp: 0.13 ± 0.011	0.357TrpTyr: 0.357 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.229TyrAla: 2.229 ± 0.043	0.332TyrCys: 0.332 ± 0.019	1.986TyrAsp: 1.986 ± 0.046	2.532TyrGlu: 2.532 ± 0.053	1.908TyrPhe: 1.908 ± 0.04	2.357TyrGly: 2.357 ± 0.049	0.711TyrHis: 0.711 ± 0.025	2.776TyrIle: 2.776 ± 0.048	2.334TyrLys: 2.334 ± 0.053	3.385TyrLeu: 3.385 ± 0.052	0.921TyrMet: 0.921 ± 0.03	1.607TyrAsn: 1.607 ± 0.039	1.272TyrPro: 1.272 ± 0.037	1.268TyrGln: 1.268 ± 0.038	1.448TyrArg: 1.448 ± 0.04	2.236TyrSer: 2.236 ± 0.047	1.909TyrThr: 1.909 ± 0.042	2.187TyrVal: 2.187 ± 0.045	0.426TyrTrp: 0.426 ± 0.018	1.461TyrTyr: 1.461 ± 0.04	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4296 proteins (1217398 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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