Methylobacterium sp. Leaf93

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium; unclassified Methylobacterium

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4228 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q4XF70|A0A0Q4XF70_9RHIZ NAD-dependent dehydratase OS=Methylobacterium sp. Leaf93 OX=1736249 GN=ASF26_09580 PE=4 SV=1
MM1 pKa = 7.22NWIEE5 pKa = 4.22LPKK8 pKa = 10.74LPTYY12 pKa = 9.69WDD14 pKa = 4.4EE15 pKa = 4.32IDD17 pKa = 4.16GSEE20 pKa = 3.82SHH22 pKa = 6.17YY23 pKa = 11.0QFGYY27 pKa = 11.09DD28 pKa = 4.99DD29 pKa = 4.92GDD31 pKa = 3.91SGDD34 pKa = 3.63SMLYY38 pKa = 9.9SGSNFKK44 pKa = 10.93YY45 pKa = 10.18YY46 pKa = 10.62EE47 pKa = 4.29EE48 pKa = 4.45YY49 pKa = 10.81DD50 pKa = 3.24EE51 pKa = 5.3GEE53 pKa = 3.79IYY55 pKa = 10.62VYY57 pKa = 10.71YY58 pKa = 10.59DD59 pKa = 3.17QIISGNIKK67 pKa = 9.65SVNYY71 pKa = 10.31NYY73 pKa = 10.44YY74 pKa = 9.94QYY76 pKa = 11.13EE77 pKa = 3.95EE78 pKa = 4.77EE79 pKa = 4.49YY80 pKa = 11.33YY81 pKa = 10.47EE82 pKa = 6.0DD83 pKa = 3.59EE84 pKa = 5.0DD85 pKa = 4.91GYY87 pKa = 11.39EE88 pKa = 3.79FSIDD92 pKa = 3.3VRR94 pKa = 11.84YY95 pKa = 10.28EE96 pKa = 3.84GAFEE100 pKa = 4.0LTKK103 pKa = 10.22IDD105 pKa = 3.39VDD107 pKa = 4.16FSEE110 pKa = 4.08ILGRR114 pKa = 11.84SAEE117 pKa = 4.23DD118 pKa = 3.16NRR120 pKa = 11.84QLVFRR125 pKa = 11.84GNDD128 pKa = 3.32QLIGSAANDD137 pKa = 3.57SLIGYY142 pKa = 8.69DD143 pKa = 4.23GNDD146 pKa = 3.06RR147 pKa = 11.84LYY149 pKa = 11.29GGGGDD154 pKa = 4.8DD155 pKa = 5.67SLDD158 pKa = 3.76GGNGSDD164 pKa = 5.06SDD166 pKa = 3.99YY167 pKa = 11.38LGNDD171 pKa = 3.12KK172 pKa = 11.12LYY174 pKa = 11.1GGSGNDD180 pKa = 3.55KK181 pKa = 10.57LAGRR185 pKa = 11.84YY186 pKa = 9.74GDD188 pKa = 4.67DD189 pKa = 3.65RR190 pKa = 11.84LEE192 pKa = 4.46GGNGNDD198 pKa = 4.44KK199 pKa = 11.03LDD201 pKa = 4.1AGSGNDD207 pKa = 3.59TLLGQDD213 pKa = 3.97GDD215 pKa = 4.83DD216 pKa = 4.03NLTSGKK222 pKa = 8.68GTDD225 pKa = 3.76ALLGGAGSDD234 pKa = 3.52ILNGGADD241 pKa = 4.12KK242 pKa = 10.01DD243 pKa = 4.18TLSGGGGKK251 pKa = 10.48GLDD254 pKa = 3.28TFVFALDD261 pKa = 3.59KK262 pKa = 10.3TAGAISRR269 pKa = 11.84SDD271 pKa = 4.47TILDD275 pKa = 3.46WNPTYY280 pKa = 11.16DD281 pKa = 5.28SIDD284 pKa = 3.35LKK286 pKa = 11.39YY287 pKa = 10.92AGTRR291 pKa = 11.84DD292 pKa = 3.31NYY294 pKa = 10.72FEE296 pKa = 4.39TRR298 pKa = 11.84TTTISAEE305 pKa = 3.95MAYY308 pKa = 10.47ARR310 pKa = 11.84YY311 pKa = 10.31DD312 pKa = 3.58GVLKK316 pKa = 10.8NDD318 pKa = 3.9DD319 pKa = 3.67MTHH322 pKa = 6.45LFLYY326 pKa = 7.94NTSTKK331 pKa = 9.77TGYY334 pKa = 10.7LLSDD338 pKa = 3.45QNADD342 pKa = 3.18HH343 pKa = 6.64MFDD346 pKa = 3.44TVVVISNAASAASMSYY362 pKa = 11.13LDD364 pKa = 3.87IVV366 pKa = 3.58

Molecular weight: 40.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q4X7V5|A0A0Q4X7V5_9RHIZ Response regulator receiver protein OS=Methylobacterium sp. Leaf93 OX=1736249 GN=ASF26_06430 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAGGRR28 pKa = 11.84KK29 pKa = 9.07VIAARR34 pKa = 11.84RR35 pKa = 11.84SHH37 pKa = 5.5GRR39 pKa = 11.84KK40 pKa = 9.35KK41 pKa = 10.69LSAA44 pKa = 3.99

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4228

0

4228

1315157

29

2686

311.1

33.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.356 ± 0.06

0.778 ± 0.013

5.791 ± 0.034

5.513 ± 0.039

3.456 ± 0.022

9.118 ± 0.044

1.939 ± 0.019

4.853 ± 0.024

2.769 ± 0.032

10.25 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.161 ± 0.016

2.234 ± 0.023

5.559 ± 0.032

2.701 ± 0.022

7.778 ± 0.043

5.418 ± 0.027

5.56 ± 0.032

7.537 ± 0.029

1.199 ± 0.015

2.03 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski