Amino acid dipepetide frequency for Methylobacterium sp. Leaf93

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.601AlaAla: 18.601 ± 0.195	1.222AlaCys: 1.222 ± 0.034	7.189AlaAsp: 7.189 ± 0.079	8.198AlaGlu: 8.198 ± 0.1	4.732AlaPhe: 4.732 ± 0.06	11.823AlaGly: 11.823 ± 0.112	2.299AlaHis: 2.299 ± 0.045	6.533AlaIle: 6.533 ± 0.08	4.05AlaLys: 4.05 ± 0.076	14.71AlaLeu: 14.71 ± 0.127	3.495AlaMet: 3.495 ± 0.062	2.691AlaAsn: 2.691 ± 0.05	6.354AlaPro: 6.354 ± 0.107	3.826AlaGln: 3.826 ± 0.061	10.137AlaArg: 10.137 ± 0.121	7.243AlaSer: 7.243 ± 0.073	6.832AlaThr: 6.832 ± 0.082	9.352AlaVal: 9.352 ± 0.083	1.603AlaTrp: 1.603 ± 0.036	2.667AlaTyr: 2.667 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	0.892CysAla: 0.892 ± 0.026	0.102CysCys: 0.102 ± 0.009	0.468CysAsp: 0.468 ± 0.02	0.424CysGlu: 0.424 ± 0.02	0.26CysPhe: 0.26 ± 0.013	0.868CysGly: 0.868 ± 0.031	0.167CysHis: 0.167 ± 0.011	0.348CysIle: 0.348 ± 0.017	0.148CysLys: 0.148 ± 0.012	0.902CysLeu: 0.902 ± 0.028	0.153CysMet: 0.153 ± 0.01	0.172CysAsn: 0.172 ± 0.011	0.445CysPro: 0.445 ± 0.023	0.176CysGln: 0.176 ± 0.012	0.663CysArg: 0.663 ± 0.024	0.388CysSer: 0.388 ± 0.018	0.402CysThr: 0.402 ± 0.02	0.574CysVal: 0.574 ± 0.022	0.093CysTrp: 0.093 ± 0.009	0.14CysTyr: 0.14 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.625AspAla: 7.625 ± 0.085	0.382AspCys: 0.382 ± 0.019	3.108AspAsp: 3.108 ± 0.058	3.443AspGlu: 3.443 ± 0.06	1.914AspPhe: 1.914 ± 0.04	5.881AspGly: 5.881 ± 0.094	1.246AspHis: 1.246 ± 0.038	2.671AspIle: 2.671 ± 0.043	1.449AspLys: 1.449 ± 0.037	6.554AspLeu: 6.554 ± 0.091	1.026AspMet: 1.026 ± 0.026	1.065AspAsn: 1.065 ± 0.036	3.82AspPro: 3.82 ± 0.063	1.4AspGln: 1.4 ± 0.037	4.863AspArg: 4.863 ± 0.063	2.176AspSer: 2.176 ± 0.038	2.915AspThr: 2.915 ± 0.077	4.247AspVal: 4.247 ± 0.055	0.853AspTrp: 0.853 ± 0.023	1.268AspTyr: 1.268 ± 0.034	0.001AspXaa: 0.001 ± 0.001
Glu	8.848GluAla: 8.848 ± 0.109	0.325GluCys: 0.325 ± 0.017	2.592GluAsp: 2.592 ± 0.045	2.92GluGlu: 2.92 ± 0.056	1.432GluPhe: 1.432 ± 0.034	4.742GluGly: 4.742 ± 0.064	1.089GluHis: 1.089 ± 0.029	3.318GluIle: 3.318 ± 0.059	1.701GluLys: 1.701 ± 0.048	4.222GluLeu: 4.222 ± 0.072	1.306GluMet: 1.306 ± 0.034	1.271GluAsn: 1.271 ± 0.033	3.069GluPro: 3.069 ± 0.056	1.579GluGln: 1.579 ± 0.043	5.497GluArg: 5.497 ± 0.077	2.426GluSer: 2.426 ± 0.045	3.803GluThr: 3.803 ± 0.057	3.647GluVal: 3.647 ± 0.054	0.566GluTrp: 0.566 ± 0.024	0.778GluTyr: 0.778 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.533PheAla: 4.533 ± 0.059	0.319PheCys: 0.319 ± 0.016	2.394PheAsp: 2.394 ± 0.042	1.997PheGlu: 1.997 ± 0.042	1.2PhePhe: 1.2 ± 0.035	3.682PheGly: 3.682 ± 0.058	0.684PheHis: 0.684 ± 0.021	1.335PheIle: 1.335 ± 0.036	0.881PheLys: 0.881 ± 0.027	3.301PheLeu: 3.301 ± 0.055	0.613PheMet: 0.613 ± 0.02	0.811PheAsn: 0.811 ± 0.024	1.543PhePro: 1.543 ± 0.038	0.867PheGln: 0.867 ± 0.028	2.266PheArg: 2.266 ± 0.036	2.118PheSer: 2.118 ± 0.043	1.975PheThr: 1.975 ± 0.045	2.814PheVal: 2.814 ± 0.053	0.445PheTrp: 0.445 ± 0.02	0.78PheTyr: 0.78 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	10.459GlyAla: 10.459 ± 0.121	0.784GlyCys: 0.784 ± 0.026	4.845GlyAsp: 4.845 ± 0.083	4.993GlyGlu: 4.993 ± 0.06	3.772GlyPhe: 3.772 ± 0.054	8.159GlyGly: 8.159 ± 0.156	1.943GlyHis: 1.943 ± 0.039	4.904GlyIle: 4.904 ± 0.064	2.673GlyLys: 2.673 ± 0.053	10.269GlyLeu: 10.269 ± 0.098	2.082GlyMet: 2.082 ± 0.043	2.105GlyAsn: 2.105 ± 0.067	4.209GlyPro: 4.209 ± 0.063	2.703GlyGln: 2.703 ± 0.045	7.461GlyArg: 7.461 ± 0.092	5.117GlySer: 5.117 ± 0.07	5.323GlyThr: 5.323 ± 0.088	5.847GlyVal: 5.847 ± 0.069	1.307GlyTrp: 1.307 ± 0.033	2.223GlyTyr: 2.223 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	2.556HisAla: 2.556 ± 0.046	0.184HisCys: 0.184 ± 0.013	1.205HisAsp: 1.205 ± 0.028	0.947HisGlu: 0.947 ± 0.03	0.741HisPhe: 0.741 ± 0.024	1.967HisGly: 1.967 ± 0.044	0.558HisHis: 0.558 ± 0.026	0.737HisIle: 0.737 ± 0.022	0.379HisLys: 0.379 ± 0.018	2.136HisLeu: 2.136 ± 0.044	0.371HisMet: 0.371 ± 0.018	0.357HisAsn: 0.357 ± 0.018	1.389HisPro: 1.389 ± 0.034	0.471HisGln: 0.471 ± 0.021	1.612HisArg: 1.612 ± 0.038	0.785HisSer: 0.785 ± 0.027	0.76HisThr: 0.76 ± 0.023	1.486HisVal: 1.486 ± 0.034	0.285HisTrp: 0.285 ± 0.014	0.461HisTyr: 0.461 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.184IleAla: 7.184 ± 0.072	0.437IleCys: 0.437 ± 0.015	3.515IleAsp: 3.515 ± 0.054	3.403IleGlu: 3.403 ± 0.053	1.257IlePhe: 1.257 ± 0.033	5.091IleGly: 5.091 ± 0.075	0.829IleHis: 0.829 ± 0.026	1.607IleIle: 1.607 ± 0.041	1.172IleLys: 1.172 ± 0.031	4.828IleLeu: 4.828 ± 0.075	0.681IleMet: 0.681 ± 0.025	1.069IleAsn: 1.069 ± 0.033	2.264IlePro: 2.264 ± 0.043	1.15IleGln: 1.15 ± 0.031	3.441IleArg: 3.441 ± 0.057	2.271IleSer: 2.271 ± 0.041	2.54IleThr: 2.54 ± 0.046	4.408IleVal: 4.408 ± 0.062	0.457IleTrp: 0.457 ± 0.02	0.928IleTyr: 0.928 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	4.248LysAla: 4.248 ± 0.072	0.109LysCys: 0.109 ± 0.009	1.519LysAsp: 1.519 ± 0.035	1.237LysGlu: 1.237 ± 0.035	0.684LysPhe: 0.684 ± 0.024	2.473LysGly: 2.473 ± 0.054	0.451LysHis: 0.451 ± 0.02	1.336LysIle: 1.336 ± 0.032	0.903LysLys: 0.903 ± 0.034	2.641LysLeu: 2.641 ± 0.053	0.517LysMet: 0.517 ± 0.022	0.65LysAsn: 0.65 ± 0.025	2.111LysPro: 2.111 ± 0.047	0.782LysGln: 0.782 ± 0.025	2.191LysArg: 2.191 ± 0.042	1.471LysSer: 1.471 ± 0.041	1.602LysThr: 1.602 ± 0.033	2.102LysVal: 2.102 ± 0.047	0.231LysTrp: 0.231 ± 0.015	0.436LysTyr: 0.436 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	15.159LeuAla: 15.159 ± 0.131	0.906LeuCys: 0.906 ± 0.027	6.724LeuAsp: 6.724 ± 0.076	4.449LeuGlu: 4.449 ± 0.064	3.432LeuPhe: 3.432 ± 0.056	8.858LeuGly: 8.858 ± 0.093	1.9LeuHis: 1.9 ± 0.044	4.712LeuIle: 4.712 ± 0.068	3.13LeuLys: 3.13 ± 0.054	9.014LeuLeu: 9.014 ± 0.104	2.084LeuMet: 2.084 ± 0.042	2.28LeuAsn: 2.28 ± 0.044	5.764LeuPro: 5.764 ± 0.078	2.427LeuGln: 2.427 ± 0.044	7.519LeuArg: 7.519 ± 0.086	6.59LeuSer: 6.59 ± 0.076	6.064LeuThr: 6.064 ± 0.075	8.378LeuVal: 8.378 ± 0.095	1.106LeuTrp: 1.106 ± 0.031	2.004LeuTyr: 2.004 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	2.845MetAla: 2.845 ± 0.047	0.116MetCys: 0.116 ± 0.009	0.915MetAsp: 0.915 ± 0.027	0.973MetGlu: 0.973 ± 0.031	0.514MetPhe: 0.514 ± 0.019	1.629MetGly: 1.629 ± 0.034	0.376MetHis: 0.376 ± 0.018	1.167MetIle: 1.167 ± 0.033	0.76MetLys: 0.76 ± 0.024	2.102MetLeu: 2.102 ± 0.042	0.572MetMet: 0.572 ± 0.019	0.646MetAsn: 0.646 ± 0.024	1.522MetPro: 1.522 ± 0.033	0.668MetGln: 0.668 ± 0.026	1.882MetArg: 1.882 ± 0.038	1.472MetSer: 1.472 ± 0.032	1.649MetThr: 1.649 ± 0.032	1.447MetVal: 1.447 ± 0.032	0.143MetTrp: 0.143 ± 0.01	0.207MetTyr: 0.207 ± 0.012	0.002MetXaa: 0.002 ± 0.001
Asn	2.897AsnAla: 2.897 ± 0.051	0.187AsnCys: 0.187 ± 0.012	1.344AsnAsp: 1.344 ± 0.046	1.071AsnGlu: 1.071 ± 0.026	0.756AsnPhe: 0.756 ± 0.028	2.306AsnGly: 2.306 ± 0.053	0.431AsnHis: 0.431 ± 0.017	0.973AsnIle: 0.973 ± 0.03	0.51AsnLys: 0.51 ± 0.02	2.319AsnLeu: 2.319 ± 0.046	0.407AsnMet: 0.407 ± 0.017	0.532AsnAsn: 0.532 ± 0.023	1.68AsnPro: 1.68 ± 0.035	0.612AsnGln: 0.612 ± 0.023	1.711AsnArg: 1.711 ± 0.042	0.928AsnSer: 0.928 ± 0.03	1.144AsnThr: 1.144 ± 0.035	1.738AsnVal: 1.738 ± 0.038	0.301AsnTrp: 0.301 ± 0.017	0.493AsnTyr: 0.493 ± 0.02	0.002AsnXaa: 0.002 ± 0.001
Pro	7.198ProAla: 7.198 ± 0.098	0.345ProCys: 0.345 ± 0.017	4.047ProAsp: 4.047 ± 0.064	3.771ProGlu: 3.771 ± 0.066	2.028ProPhe: 2.028 ± 0.036	5.161ProGly: 5.161 ± 0.076	1.089ProHis: 1.089 ± 0.026	2.343ProIle: 2.343 ± 0.043	1.662ProLys: 1.662 ± 0.039	4.993ProLeu: 4.993 ± 0.069	1.235ProMet: 1.235 ± 0.029	1.29ProAsn: 1.29 ± 0.033	3.078ProPro: 3.078 ± 0.064	1.436ProGln: 1.436 ± 0.036	3.503ProArg: 3.503 ± 0.066	3.248ProSer: 3.248 ± 0.057	2.675ProThr: 2.675 ± 0.045	4.661ProVal: 4.661 ± 0.069	0.707ProTrp: 0.707 ± 0.025	1.115ProTyr: 1.115 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	4.158GlnAla: 4.158 ± 0.058	0.151GlnCys: 0.151 ± 0.011	1.408GlnAsp: 1.408 ± 0.032	1.166GlnGlu: 1.166 ± 0.031	0.867GlnPhe: 0.867 ± 0.027	2.463GlnGly: 2.463 ± 0.051	0.475GlnHis: 0.475 ± 0.019	1.543GlnIle: 1.543 ± 0.037	0.77GlnLys: 0.77 ± 0.027	2.083GlnLeu: 2.083 ± 0.051	0.595GlnMet: 0.595 ± 0.022	0.684GlnAsn: 0.684 ± 0.022	1.607GlnPro: 1.607 ± 0.034	0.836GlnGln: 0.836 ± 0.034	2.159GlnArg: 2.159 ± 0.046	1.585GlnSer: 1.585 ± 0.039	1.608GlnThr: 1.608 ± 0.039	2.095GlnVal: 2.095 ± 0.043	0.29GlnTrp: 0.29 ± 0.015	0.471GlnTyr: 0.471 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	9.113ArgAla: 9.113 ± 0.101	0.509ArgCys: 0.509 ± 0.019	4.596ArgAsp: 4.596 ± 0.066	4.293ArgGlu: 4.293 ± 0.069	3.105ArgPhe: 3.105 ± 0.052	5.586ArgGly: 5.586 ± 0.072	1.782ArgHis: 1.782 ± 0.043	4.482ArgIle: 4.482 ± 0.053	1.937ArgLys: 1.937 ± 0.041	8.922ArgLeu: 8.922 ± 0.103	1.882ArgMet: 1.882 ± 0.037	1.782ArgAsn: 1.782 ± 0.037	4.408ArgPro: 4.408 ± 0.077	2.295ArgGln: 2.295 ± 0.046	6.826ArgArg: 6.826 ± 0.087	4.283ArgSer: 4.283 ± 0.06	3.938ArgThr: 3.938 ± 0.066	5.323ArgVal: 5.323 ± 0.068	0.972ArgTrp: 0.972 ± 0.03	1.741ArgTyr: 1.741 ± 0.04	0.001ArgXaa: 0.001 ± 0.001
Ser	6.697SerAla: 6.697 ± 0.085	0.371SerCys: 0.371 ± 0.018	3.104SerAsp: 3.104 ± 0.052	2.874SerGlu: 2.874 ± 0.052	2.013SerPhe: 2.013 ± 0.038	5.819SerGly: 5.819 ± 0.077	1.062SerHis: 1.062 ± 0.027	2.443SerIle: 2.443 ± 0.044	1.334SerLys: 1.334 ± 0.036	5.938SerLeu: 5.938 ± 0.068	1.126SerMet: 1.126 ± 0.031	1.183SerAsn: 1.183 ± 0.031	3.194SerPro: 3.194 ± 0.05	1.484SerGln: 1.484 ± 0.035	3.958SerArg: 3.958 ± 0.061	2.869SerSer: 2.869 ± 0.055	2.769SerThr: 2.769 ± 0.056	4.117SerVal: 4.117 ± 0.06	0.624SerTrp: 0.624 ± 0.022	1.2SerTyr: 1.2 ± 0.035	0.0SerXaa: 0.0 ± 0.0
Thr	6.573ThrAla: 6.573 ± 0.079	0.424ThrCys: 0.424 ± 0.02	2.907ThrAsp: 2.907 ± 0.05	2.653ThrGlu: 2.653 ± 0.049	1.996ThrPhe: 1.996 ± 0.043	5.688ThrGly: 5.688 ± 0.081	1.001ThrHis: 1.001 ± 0.029	3.003ThrIle: 3.003 ± 0.058	1.432ThrLys: 1.432 ± 0.038	6.533ThrLeu: 6.533 ± 0.083	1.115ThrMet: 1.115 ± 0.033	1.227ThrAsn: 1.227 ± 0.033	3.397ThrPro: 3.397 ± 0.061	1.423ThrGln: 1.423 ± 0.04	3.797ThrArg: 3.797 ± 0.053	2.874ThrSer: 2.874 ± 0.054	2.926ThrThr: 2.926 ± 0.049	4.685ThrVal: 4.685 ± 0.075	0.691ThrTrp: 0.691 ± 0.021	1.256ThrTyr: 1.256 ± 0.034	0.001ThrXaa: 0.001 ± 0.001
Val	10.223ValAla: 10.223 ± 0.097	0.595ValCys: 0.595 ± 0.021	4.114ValAsp: 4.114 ± 0.067	4.7ValGlu: 4.7 ± 0.063	2.616ValPhe: 2.616 ± 0.048	5.892ValGly: 5.892 ± 0.065	1.308ValHis: 1.308 ± 0.035	3.763ValIle: 3.763 ± 0.061	2.016ValLys: 2.016 ± 0.044	7.418ValLeu: 7.418 ± 0.09	1.744ValMet: 1.744 ± 0.033	1.686ValAsn: 1.686 ± 0.042	4.143ValPro: 4.143 ± 0.063	1.924ValGln: 1.924 ± 0.036	5.269ValArg: 5.269 ± 0.066	4.558ValSer: 4.558 ± 0.059	4.886ValThr: 4.886 ± 0.075	6.255ValVal: 6.255 ± 0.092	0.836ValTrp: 0.836 ± 0.025	1.422ValTyr: 1.422 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	1.166TrpAla: 1.166 ± 0.031	0.12TrpCys: 0.12 ± 0.009	0.557TrpAsp: 0.557 ± 0.023	0.456TrpGlu: 0.456 ± 0.02	0.495TrpPhe: 0.495 ± 0.017	0.836TrpGly: 0.836 ± 0.028	0.294TrpHis: 0.294 ± 0.014	0.643TrpIle: 0.643 ± 0.023	0.329TrpLys: 0.329 ± 0.016	1.459TrpLeu: 1.459 ± 0.042	0.304TrpMet: 0.304 ± 0.014	0.36TrpAsn: 0.36 ± 0.016	0.63TrpPro: 0.63 ± 0.023	0.395TrpGln: 0.395 ± 0.019	1.19TrpArg: 1.19 ± 0.03	0.817TrpSer: 0.817 ± 0.028	0.775TrpThr: 0.775 ± 0.026	0.705TrpVal: 0.705 ± 0.025	0.208TrpTrp: 0.208 ± 0.014	0.248TrpTyr: 0.248 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.583TyrAla: 2.583 ± 0.05	0.196TyrCys: 0.196 ± 0.012	1.356TyrAsp: 1.356 ± 0.033	1.162TyrGlu: 1.162 ± 0.031	0.744TyrPhe: 0.744 ± 0.026	2.257TyrGly: 2.257 ± 0.051	0.403TyrHis: 0.403 ± 0.017	0.672TyrIle: 0.672 ± 0.023	0.457TyrLys: 0.457 ± 0.02	2.154TyrLeu: 2.154 ± 0.049	0.3TyrMet: 0.3 ± 0.015	0.479TyrAsn: 0.479 ± 0.019	0.959TyrPro: 0.959 ± 0.028	0.557TyrGln: 0.557 ± 0.02	1.832TyrArg: 1.832 ± 0.035	0.959TyrSer: 0.959 ± 0.031	1.013TyrThr: 1.013 ± 0.028	1.486TyrVal: 1.486 ± 0.034	0.27TyrTrp: 0.27 ± 0.015	0.466TyrTyr: 0.466 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4228 proteins (1315157 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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