Halorussus sp. MSC15.2

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Halobacteriaceae; Halorussus; unclassified Halorussus

Average proteome isoelectric point is 4.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4072 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G3ZHY4|A0A6G3ZHY4_9EURY Metal-dependent hydrolase OS=Halorussus sp. MSC15.2 OX=2283638 GN=FXF75_04805 PE=4 SV=1
MM1 pKa = 7.29NRR3 pKa = 11.84RR4 pKa = 11.84TFLSALATAAGSSAAGIEE22 pKa = 3.94LTGRR26 pKa = 11.84SRR28 pKa = 11.84AASGRR33 pKa = 11.84IPEE36 pKa = 4.44LEE38 pKa = 4.42AYY40 pKa = 7.58STSSLVTYY48 pKa = 10.06DD49 pKa = 3.91YY50 pKa = 11.84APLTDD55 pKa = 4.45DD56 pKa = 3.26AHH58 pKa = 5.63VAVWAEE64 pKa = 4.05DD65 pKa = 3.42TATNGDD71 pKa = 3.81GDD73 pKa = 3.99TSADD77 pKa = 3.21AVSYY81 pKa = 11.54GEE83 pKa = 4.3DD84 pKa = 3.33TPIPVVASDD93 pKa = 3.48WNVVGFGSMLVTDD106 pKa = 5.81ADD108 pKa = 4.36TDD110 pKa = 3.6WQTGNEE116 pKa = 3.97EE117 pKa = 3.91FVLNVWDD124 pKa = 4.58DD125 pKa = 3.73ALGGSGTVLWDD136 pKa = 3.6EE137 pKa = 4.43GHH139 pKa = 5.63GQYY142 pKa = 8.64YY143 pKa = 8.68TLSKK147 pKa = 10.28FAEE150 pKa = 3.98FDD152 pKa = 3.63GYY154 pKa = 11.71AEE156 pKa = 4.17NNGYY160 pKa = 9.99AVSATTSLTADD171 pKa = 4.35LSAADD176 pKa = 3.95AAVITSPSTAFTQSEE191 pKa = 4.4LSALGDD197 pKa = 3.78FVSNGGWLFLHH208 pKa = 6.8DD209 pKa = 4.62QSDD212 pKa = 3.46HH213 pKa = 6.44SNYY216 pKa = 10.84DD217 pKa = 3.02EE218 pKa = 4.15TANLNDD224 pKa = 3.67VASHH228 pKa = 6.81LEE230 pKa = 3.67LAFRR234 pKa = 11.84FNDD237 pKa = 3.57DD238 pKa = 3.07QVTDD242 pKa = 4.53EE243 pKa = 4.54IRR245 pKa = 11.84NGGEE249 pKa = 3.62PYY251 pKa = 10.59QPVTTQFNTAFSYY264 pKa = 10.84FGDD267 pKa = 3.66RR268 pKa = 11.84DD269 pKa = 3.94GLGLDD274 pKa = 3.52PEE276 pKa = 4.38KK277 pKa = 10.3TYY279 pKa = 10.14TVSVTEE285 pKa = 4.71VIDD288 pKa = 4.0GDD290 pKa = 4.36TVKK293 pKa = 9.43VTFDD297 pKa = 4.15DD298 pKa = 4.22GTTEE302 pKa = 4.1NIRR305 pKa = 11.84ILGTDD310 pKa = 3.48TPEE313 pKa = 4.09KK314 pKa = 10.5SSNSQYY320 pKa = 10.93EE321 pKa = 4.15RR322 pKa = 11.84VEE324 pKa = 3.76EE325 pKa = 4.26WEE327 pKa = 4.74GIEE330 pKa = 4.49SNTYY334 pKa = 10.44LEE336 pKa = 4.76ARR338 pKa = 11.84ADD340 pKa = 3.58DD341 pKa = 3.57ATNFGKK347 pKa = 10.25SEE349 pKa = 4.21LGGKK353 pKa = 9.45SVDD356 pKa = 4.37LAFDD360 pKa = 4.1DD361 pKa = 4.4NEE363 pKa = 4.19PVRR366 pKa = 11.84DD367 pKa = 3.56AFDD370 pKa = 3.51RR371 pKa = 11.84VLGYY375 pKa = 9.93IYY377 pKa = 10.82YY378 pKa = 10.22DD379 pKa = 3.29ASGDD383 pKa = 3.76GNRR386 pKa = 11.84DD387 pKa = 2.74ANYY390 pKa = 10.3NHH392 pKa = 7.14RR393 pKa = 11.84LVKK396 pKa = 10.44NGHH399 pKa = 5.69ARR401 pKa = 11.84VYY403 pKa = 10.42DD404 pKa = 3.68SSFAKK409 pKa = 10.27HH410 pKa = 6.32DD411 pKa = 3.79AFVDD415 pKa = 3.76SEE417 pKa = 4.49TTARR421 pKa = 11.84ADD423 pKa = 3.62GTQVWAEE430 pKa = 4.07SDD432 pKa = 3.51PGNSTEE438 pKa = 4.83IRR440 pKa = 11.84DD441 pKa = 3.97NPVDD445 pKa = 4.9DD446 pKa = 5.61LFFPQTASVRR456 pKa = 11.84TASGGVPDD464 pKa = 4.24SRR466 pKa = 11.84VPVYY470 pKa = 10.84AEE472 pKa = 3.84STATQDD478 pKa = 3.51LDD480 pKa = 3.29GGYY483 pKa = 9.86AYY485 pKa = 10.83SGDD488 pKa = 3.83IPLVAVDD495 pKa = 3.71EE496 pKa = 4.58DD497 pKa = 4.63ANVGVVGGPFIDD509 pKa = 3.81EE510 pKa = 4.45SYY512 pKa = 10.37EE513 pKa = 3.96KK514 pKa = 10.95SEE516 pKa = 4.71GYY518 pKa = 8.14STDD521 pKa = 2.89TSSYY525 pKa = 11.07GNFAFLTNLVDD536 pKa = 3.97YY537 pKa = 10.85LADD540 pKa = 3.44TSGDD544 pKa = 3.62VLVDD548 pKa = 3.81GGHH551 pKa = 5.71GQFGASYY558 pKa = 9.97GLSAEE563 pKa = 3.9DD564 pKa = 2.77TAYY567 pKa = 10.94YY568 pKa = 9.5MRR570 pKa = 11.84YY571 pKa = 10.21LEE573 pKa = 4.58GQDD576 pKa = 3.38VALEE580 pKa = 4.2GVNEE584 pKa = 3.97FTGANLDD591 pKa = 3.44GARR594 pKa = 11.84AVVVTTPPEE603 pKa = 3.93SFTQSEE609 pKa = 3.92IDD611 pKa = 3.41ALNDD615 pKa = 3.48FVADD619 pKa = 4.2GGAVVLVGSGKK630 pKa = 6.84TTAAARR636 pKa = 11.84ANVNDD641 pKa = 4.25LADD644 pKa = 4.06GLGTDD649 pKa = 4.16LQVNADD655 pKa = 3.93RR656 pKa = 11.84VLDD659 pKa = 3.91DD660 pKa = 4.02TNSVATSPEE669 pKa = 4.1VPEE672 pKa = 4.08TTVFDD677 pKa = 4.02GSFPLFDD684 pKa = 5.11AYY686 pKa = 9.97TPGTSSDD693 pKa = 3.7YY694 pKa = 10.94SVSIPTISEE703 pKa = 4.32DD704 pKa = 4.15GSTLDD709 pKa = 5.2DD710 pKa = 4.27EE711 pKa = 4.9YY712 pKa = 11.99VDD714 pKa = 3.84VKK716 pKa = 11.39NDD718 pKa = 3.15GSSAQDD724 pKa = 3.27LTGWTLEE731 pKa = 4.2DD732 pKa = 3.93EE733 pKa = 4.98AGHH736 pKa = 6.36TYY738 pKa = 10.47GFPDD742 pKa = 4.54GFSLGAGDD750 pKa = 3.9TVRR753 pKa = 11.84VHH755 pKa = 6.41TGSGTDD761 pKa = 3.41SSTDD765 pKa = 3.67LYY767 pKa = 9.93WDD769 pKa = 3.62RR770 pKa = 11.84GSAVWNDD777 pKa = 3.58GGDD780 pKa = 3.51TASVYY785 pKa = 11.35DD786 pKa = 4.24DD787 pKa = 4.53AGNLAAEE794 pKa = 4.39KK795 pKa = 9.63TYY797 pKa = 8.56PTNDD801 pKa = 3.52SDD803 pKa = 4.88SKK805 pKa = 10.81IAVKK809 pKa = 9.33TVSEE813 pKa = 4.43AGSTLNDD820 pKa = 3.24EE821 pKa = 4.64YY822 pKa = 11.84VDD824 pKa = 4.22FEE826 pKa = 4.56NTGSSAQDD834 pKa = 3.23LTGWTVEE841 pKa = 4.33DD842 pKa = 4.61EE843 pKa = 4.36ADD845 pKa = 3.34HH846 pKa = 6.79TYY848 pKa = 11.14GFPDD852 pKa = 4.39GFSLGAGEE860 pKa = 4.24TVRR863 pKa = 11.84LHH865 pKa = 6.76SGDD868 pKa = 3.78GTDD871 pKa = 4.94SATDD875 pKa = 4.24LYY877 pKa = 10.73WGGSYY882 pKa = 9.51VWNDD886 pKa = 3.08GGDD889 pKa = 3.64TVSLYY894 pKa = 10.84DD895 pKa = 4.56AGGTLHH901 pKa = 6.62TEE903 pKa = 3.69YY904 pKa = 10.85SYY906 pKa = 12.1

Molecular weight: 96.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G3ZER7|A0A6G3ZER7_9EURY Uncharacterized protein OS=Halorussus sp. MSC15.2 OX=2283638 GN=FXF75_00940 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.46LNTRR6 pKa = 11.84IRR8 pKa = 11.84RR9 pKa = 11.84TGPASRR15 pKa = 11.84TRR17 pKa = 11.84RR18 pKa = 11.84GRR20 pKa = 11.84IASGVTRR27 pKa = 11.84FALPVATLLALVASALLTGVTFATVAALLPMPVPLLLGGWVAAIGLTFALPLVVVRR83 pKa = 11.84GVVALLEE90 pKa = 4.35RR91 pKa = 11.84FQQ93 pKa = 4.59

Molecular weight: 9.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4072

0

4072

1157722

44

2031

284.3

30.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.692 ± 0.054

0.662 ± 0.013

8.269 ± 0.044

8.683 ± 0.06

3.426 ± 0.027

8.554 ± 0.041

1.895 ± 0.021

3.383 ± 0.029

2.085 ± 0.026

8.677 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.709 ± 0.016

2.59 ± 0.024

4.527 ± 0.025

2.496 ± 0.024

6.71 ± 0.039

5.847 ± 0.041

6.537 ± 0.049

9.306 ± 0.042

1.186 ± 0.013

2.769 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski