Pseudomonas taeanensis MS-3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; Pseudomonas taeanensis

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4573 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1YFP6|A0A0A1YFP6_9PSED DeoR faimly transcriptional regulator OS=Pseudomonas taeanensis MS-3 OX=1395571 GN=TMS3_0114685 PE=4 SV=1
MM1 pKa = 7.46LLDD4 pKa = 3.74EE5 pKa = 4.69TAGALDD11 pKa = 3.83PTIGFPTAGLSFSPEE26 pKa = 3.84FPDD29 pKa = 5.17PEE31 pKa = 4.69LEE33 pKa = 4.26PDD35 pKa = 3.62VTPEE39 pKa = 3.43PTAAVVPEE47 pKa = 5.09GPDD50 pKa = 3.15STPEE54 pKa = 3.7VEE56 pKa = 5.96IIHH59 pKa = 6.19QDD61 pKa = 3.01AEE63 pKa = 4.56GQVLGAPGVVDD74 pKa = 4.43EE75 pKa = 5.18AALATGSNAASNAEE89 pKa = 3.98QAFGRR94 pKa = 11.84LVVNSPDD101 pKa = 3.17GVAALQVQDD110 pKa = 5.3KK111 pKa = 10.43DD112 pKa = 4.33GNWVDD117 pKa = 3.62VTAGGTVQGVYY128 pKa = 10.92GSLVVDD134 pKa = 4.1AAGRR138 pKa = 11.84WVYY141 pKa = 9.37TLNGSTQDD149 pKa = 3.49HH150 pKa = 6.84GNPSAIGAADD160 pKa = 3.41QVGEE164 pKa = 4.24SFAVRR169 pKa = 11.84VFDD172 pKa = 3.59TDD174 pKa = 3.79GDD176 pKa = 4.01VSPVSTLSININDD189 pKa = 4.78DD190 pKa = 3.39GPNAVDD196 pKa = 4.21DD197 pKa = 4.56SNSIAEE203 pKa = 4.36DD204 pKa = 3.45TLTPISGNVLSNDD217 pKa = 3.44LHH219 pKa = 7.1PNGQPGADD227 pKa = 3.43TPISFVSWTSTAATYY242 pKa = 7.93GTFTDD247 pKa = 4.4TGNGTYY253 pKa = 9.97SYY255 pKa = 11.06SLNNANPAVQGLDD268 pKa = 3.1AGEE271 pKa = 4.39SLSEE275 pKa = 4.17TFSYY279 pKa = 10.45TMQDD283 pKa = 2.85ADD285 pKa = 4.37GDD287 pKa = 4.28TATATLTITITGSNDD302 pKa = 3.14VPVLTVDD309 pKa = 4.82PGNQGANDD317 pKa = 3.64RR318 pKa = 11.84VFEE321 pKa = 4.73AGLAQGSDD329 pKa = 3.03AASNSEE335 pKa = 4.07FASGTFTLSDD345 pKa = 3.83ADD347 pKa = 4.62GLDD350 pKa = 3.99DD351 pKa = 4.69LQSVTINGVTVAIGSLAGSQFIGAHH376 pKa = 5.67GSLTISAYY384 pKa = 9.04NTATGVASYY393 pKa = 10.35SYY395 pKa = 10.54EE396 pKa = 4.05LTSPTTDD403 pKa = 3.14GPGIEE408 pKa = 4.45SDD410 pKa = 3.76SFTLSVSDD418 pKa = 3.92GTASSAPASIVIEE431 pKa = 4.05IVDD434 pKa = 4.32DD435 pKa = 4.02VPHH438 pKa = 7.02AVDD441 pKa = 4.76DD442 pKa = 4.54SNATFASEE450 pKa = 4.08NQLTLIGNVLDD461 pKa = 4.73NDD463 pKa = 3.86VQGADD468 pKa = 4.2RR469 pKa = 11.84LPGGPIQPAVMQGTYY484 pKa = 8.95GTLTLNADD492 pKa = 3.47GSYY495 pKa = 10.73SYY497 pKa = 11.45LLDD500 pKa = 4.4PSDD503 pKa = 4.96ADD505 pKa = 3.97FLALGGGGMATEE517 pKa = 4.72TFTYY521 pKa = 9.52TLNDD525 pKa = 3.66ADD527 pKa = 4.77GDD529 pKa = 4.2SDD531 pKa = 4.0TANLVLKK538 pKa = 10.34VRR540 pKa = 11.84NLDD543 pKa = 3.59DD544 pKa = 3.6KK545 pKa = 11.81VQIKK549 pKa = 10.69DD550 pKa = 3.19LDD552 pKa = 3.81VRR554 pKa = 11.84GGEE557 pKa = 4.09HH558 pKa = 7.05RR559 pKa = 11.84VDD561 pKa = 3.86EE562 pKa = 5.62DD563 pKa = 3.93DD564 pKa = 4.84LAMGSDD570 pKa = 3.81PSKK573 pKa = 10.99EE574 pKa = 4.06STTVSGTFKK583 pKa = 10.86VQAPDD588 pKa = 3.17GLLNLTVGGIQVVLNGVVSGFPQSVTTPLGNQLTITGFDD627 pKa = 3.67ADD629 pKa = 5.73DD630 pKa = 5.42GILDD634 pKa = 3.99EE635 pKa = 5.97GIVSYY640 pKa = 10.82SYY642 pKa = 10.23TLLGSAQHH650 pKa = 6.46APGGGEE656 pKa = 3.91NQLTEE661 pKa = 4.26HH662 pKa = 6.43FAVLAEE668 pKa = 4.71DD669 pKa = 3.94VDD671 pKa = 4.15HH672 pKa = 7.23DD673 pKa = 4.26TDD675 pKa = 5.38LASLDD680 pKa = 3.55INIIDD685 pKa = 4.48DD686 pKa = 3.74VPHH689 pKa = 6.91AVDD692 pKa = 4.43DD693 pKa = 4.54SNSIAEE699 pKa = 4.47DD700 pKa = 3.42SVTPITGNVLSNDD713 pKa = 3.59LHH715 pKa = 7.1PNGQPGADD723 pKa = 3.37TPTSFVGWTSTAATYY738 pKa = 7.93GTFTDD743 pKa = 4.4TGNGTYY749 pKa = 9.97SYY751 pKa = 11.06SLNNANPAVQGLNTGEE767 pKa = 4.3SLSEE771 pKa = 3.96TFTYY775 pKa = 9.93TMRR778 pKa = 11.84DD779 pKa = 3.15ADD781 pKa = 4.37GDD783 pKa = 3.96TATAKK788 pKa = 9.58LTITINGADD797 pKa = 3.59DD798 pKa = 3.67HH799 pKa = 7.44ASVVTAAAYY808 pKa = 10.16GPDD811 pKa = 3.07ATVYY815 pKa = 9.82EE816 pKa = 4.62HH817 pKa = 7.16GLTSLADD824 pKa = 3.75TSEE827 pKa = 4.3STSGSFTVSASDD839 pKa = 4.65GIASVTVGGATFSLAQLQALAATNQLVSTGEE870 pKa = 4.06GTLTLTGYY878 pKa = 10.07SGSATAGTLSYY889 pKa = 10.89SYY891 pKa = 9.71TLNATIDD898 pKa = 3.87NDD900 pKa = 3.68SHH902 pKa = 8.18AGATDD907 pKa = 3.07THH909 pKa = 7.4FDD911 pKa = 3.68DD912 pKa = 4.41SLSISVLGVGGSTASDD928 pKa = 3.4EE929 pKa = 4.27LVVRR933 pKa = 11.84IIDD936 pKa = 3.88DD937 pKa = 3.8TPTAMNDD944 pKa = 3.43GPVGVTEE951 pKa = 4.44DD952 pKa = 3.62AVSNSVGGNVLTNDD966 pKa = 3.35SAGADD971 pKa = 3.61TPKK974 pKa = 11.09AFTTWAATGHH984 pKa = 7.0DD985 pKa = 3.49NTAAINALNSFGTLTLAAGGVYY1007 pKa = 10.41SFVLDD1012 pKa = 3.54NSLASVQALNASSHH1026 pKa = 5.97LSYY1029 pKa = 11.04DD1030 pKa = 3.22VYY1032 pKa = 11.01YY1033 pKa = 10.86TMQDD1037 pKa = 2.88ADD1039 pKa = 4.36GDD1041 pKa = 4.2SSSAKK1046 pKa = 9.41LTITISGADD1055 pKa = 3.43DD1056 pKa = 3.56HH1057 pKa = 7.52ASVVTAAAYY1066 pKa = 10.16GPDD1069 pKa = 3.07ATVYY1073 pKa = 9.82EE1074 pKa = 4.62HH1075 pKa = 7.16GLTSLADD1082 pKa = 3.68TSEE1085 pKa = 4.31TTSGSFTVSASDD1097 pKa = 4.65GIASVTVGGATFSFAQLQALATTNQQVSTGEE1128 pKa = 4.14GTLTLSNYY1136 pKa = 10.1SGSATAGTLSYY1147 pKa = 10.89SYY1149 pKa = 9.71TLNATIDD1156 pKa = 3.72NDD1158 pKa = 3.9SHH1160 pKa = 8.04VGATGTYY1167 pKa = 9.76FDD1169 pKa = 5.49DD1170 pKa = 3.94SLSISVMGVGGSAASDD1186 pKa = 3.59DD1187 pKa = 4.01LVVRR1191 pKa = 11.84IVDD1194 pKa = 3.75DD1195 pKa = 4.12TPTLSTVSPASLSNNATGFATGTSDD1220 pKa = 3.54PVIGADD1226 pKa = 3.78VPGTADD1232 pKa = 3.34LTGNIAGWNGTSVTYY1247 pKa = 10.24AASSLTSSGNTVYY1260 pKa = 10.98YY1261 pKa = 10.46AVSPVDD1267 pKa = 3.21TGVLYY1272 pKa = 10.4AYY1274 pKa = 9.19TSALPGTYY1282 pKa = 9.18TGGPGQSLIFTLTYY1296 pKa = 10.67DD1297 pKa = 3.15AAGNYY1302 pKa = 9.73AIDD1305 pKa = 3.86MNGKK1309 pKa = 9.64LDD1311 pKa = 4.21GPTQSFDD1318 pKa = 2.99ATFNQNIGGNQDD1330 pKa = 3.44YY1331 pKa = 11.25LLVTDD1336 pKa = 4.5TGALYY1341 pKa = 10.48KK1342 pKa = 10.26PGEE1345 pKa = 4.68SIPLGQTVIMTVDD1358 pKa = 3.18SSEE1361 pKa = 4.36GGVNSSMQGLAPDD1374 pKa = 4.02NQWVDD1379 pKa = 3.09GSEE1382 pKa = 4.31VIFFSYY1388 pKa = 10.85ASPIVSAQFSIDD1400 pKa = 3.21IQGGGSSNTVHH1411 pKa = 5.23WTVYY1415 pKa = 10.46GQDD1418 pKa = 3.41SLGNTTTQSGNTLFSDD1434 pKa = 3.97HH1435 pKa = 6.8VLTSIPTGLTAITRR1449 pKa = 11.84IDD1451 pKa = 3.68LSDD1454 pKa = 3.14TGGNGFRR1461 pKa = 11.84VNGSSIVEE1469 pKa = 4.42RR1470 pKa = 11.84IEE1472 pKa = 3.86EE1473 pKa = 4.25DD1474 pKa = 3.99PIATQFEE1481 pKa = 4.77VAVIDD1486 pKa = 4.23ADD1488 pKa = 3.75GDD1490 pKa = 3.62RR1491 pKa = 11.84AAAVLDD1497 pKa = 4.43VIFEE1501 pKa = 4.28PSLTGQLIVASNASDD1516 pKa = 3.62VAGSSAPYY1524 pKa = 8.22VTSGGAGVITGGAAADD1540 pKa = 3.49ILVGDD1545 pKa = 4.55EE1546 pKa = 4.11GRR1548 pKa = 11.84SNLVGKK1554 pKa = 9.56SVNLILILDD1563 pKa = 3.88SSGSMKK1569 pKa = 10.94ANIAFNDD1576 pKa = 3.73TNMSRR1581 pKa = 11.84MEE1583 pKa = 4.12ALKK1586 pKa = 10.79LAVNNLLVGLSLGAADD1602 pKa = 3.88HH1603 pKa = 6.53VRR1605 pKa = 11.84VQLVDD1610 pKa = 4.32FDD1612 pKa = 4.89SSASSLGIFDD1622 pKa = 5.3LKK1624 pKa = 11.08DD1625 pKa = 3.63GEE1627 pKa = 4.5LSAAQSAVNSLVAGGYY1643 pKa = 7.4TNYY1646 pKa = 10.41EE1647 pKa = 3.81AGLQVALNWVASGGVDD1663 pKa = 4.23APYY1666 pKa = 10.23TGANVINQVVFVSDD1680 pKa = 4.14GEE1682 pKa = 4.41PNSWLNGDD1690 pKa = 3.84STSLSNVTTDD1700 pKa = 3.33GSKK1703 pKa = 8.92STAVAQVLGSYY1714 pKa = 10.03NPSGHH1719 pKa = 6.75INDD1722 pKa = 4.28DD1723 pKa = 3.61RR1724 pKa = 11.84VSEE1727 pKa = 4.26VALLEE1732 pKa = 4.38STFGQIQAVGINVGGTALDD1751 pKa = 3.36ILNQVEE1757 pKa = 4.34GEE1759 pKa = 4.16LANVNPDD1766 pKa = 3.0VASNITTGEE1775 pKa = 3.81QLASVLADD1783 pKa = 4.0FNPSNQLHH1791 pKa = 6.9DD1792 pKa = 4.71AGSDD1796 pKa = 3.62HH1797 pKa = 6.84IVGGGGDD1804 pKa = 4.09DD1805 pKa = 5.64LIFGDD1810 pKa = 4.46VLYY1813 pKa = 10.47TDD1815 pKa = 4.42VLAATAGLTTNPGAGWQVFAALEE1838 pKa = 4.56GGDD1841 pKa = 4.19GPGSYY1846 pKa = 9.0ATWTRR1851 pKa = 11.84ADD1853 pKa = 3.49TLNYY1857 pKa = 9.51IRR1859 pKa = 11.84THH1861 pKa = 7.06AEE1863 pKa = 3.5EE1864 pKa = 4.2LAGEE1868 pKa = 4.24SGRR1871 pKa = 11.84ALGHH1875 pKa = 6.46DD1876 pKa = 4.61RR1877 pKa = 11.84IDD1879 pKa = 3.9AGDD1882 pKa = 3.97GNDD1885 pKa = 3.88LVFGQEE1891 pKa = 3.95GNDD1894 pKa = 3.63YY1895 pKa = 11.01ILGGKK1900 pKa = 9.59GNDD1903 pKa = 3.36VLAGGSGHH1911 pKa = 6.8DD1912 pKa = 3.61TLIGGEE1918 pKa = 4.23GDD1920 pKa = 3.64DD1921 pKa = 4.02VLIGGSGNDD1930 pKa = 4.03LLSGGLGKK1938 pKa = 10.32DD1939 pKa = 3.2SFVWNSGDD1947 pKa = 3.61SGIDD1951 pKa = 3.08HH1952 pKa = 6.01VTDD1955 pKa = 4.25FFIDD1959 pKa = 4.05GPNVSGGNSDD1969 pKa = 4.74VLDD1972 pKa = 4.41LSQLLVGEE1980 pKa = 4.44SASGNVLDD1988 pKa = 5.88DD1989 pKa = 3.73YY1990 pKa = 11.87LSFTFGASTAIDD2002 pKa = 3.51VRR2004 pKa = 11.84ATAGGAVVQQVVLDD2018 pKa = 4.22GVDD2021 pKa = 3.73LSSAAYY2027 pKa = 10.11YY2028 pKa = 10.89GSTDD2032 pKa = 3.26AATVIDD2038 pKa = 5.77GLLADD2043 pKa = 4.48NALKK2047 pKa = 10.72VDD2049 pKa = 3.98TVV2051 pKa = 3.3

Molecular weight: 208.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1YGA6|A0A0A1YGA6_9PSED Uncharacterized protein OS=Pseudomonas taeanensis MS-3 OX=1395571 GN=TMS3_0120440 PE=4 SV=1
MM1 pKa = 7.78PKK3 pKa = 10.48LPTALQHH10 pKa = 5.63WRR12 pKa = 11.84SWWLTPLIGLVGGYY26 pKa = 8.99LASLIGWPLPWIIGSLLAVIAARR49 pKa = 11.84CSGWRR54 pKa = 11.84VTEE57 pKa = 4.12LPGGRR62 pKa = 11.84QTGQWLVASGIGLHH76 pKa = 5.39FTGAVMAQVLSHH88 pKa = 6.83FPLILAGALGTLLLSLIGIALLRR111 pKa = 11.84RR112 pKa = 11.84AGVDD116 pKa = 2.61RR117 pKa = 11.84ATAFFASMPGGASEE131 pKa = 4.42MVNLAQRR138 pKa = 11.84HH139 pKa = 5.45DD140 pKa = 3.65AQIARR145 pKa = 11.84VAAAHH150 pKa = 5.84SLRR153 pKa = 11.84LLLVVLLIPALFTWSLAPIQAPAPAPVDD181 pKa = 3.92WYY183 pKa = 9.94WLALLLPGGALLALLWKK200 pKa = 10.33KK201 pKa = 10.12LRR203 pKa = 11.84QPNPWMLGPLTVCAAASVTFDD224 pKa = 4.07LQLGLPPGLGQVGQWLIGCALGCHH248 pKa = 6.53FDD250 pKa = 3.34RR251 pKa = 11.84AFFRR255 pKa = 11.84SAPGFLARR263 pKa = 11.84ILLFSLLAMLGAALLAQGLSWLASQDD289 pKa = 3.39GTSLMLGMMPGGITEE304 pKa = 4.67LCLTAEE310 pKa = 4.39ALQLSVALVTALQVLRR326 pKa = 11.84LFLVMFLAEE335 pKa = 4.03PLFRR339 pKa = 11.84LWQRR343 pKa = 11.84SS344 pKa = 3.29

Molecular weight: 36.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4573

0

4573

1480677

35

2430

323.8

35.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.192 ± 0.046

1.062 ± 0.014

5.182 ± 0.027

5.94 ± 0.032

3.635 ± 0.022

7.882 ± 0.034

2.307 ± 0.019

4.741 ± 0.027

3.292 ± 0.027

12.282 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.284 ± 0.02

2.876 ± 0.021

4.764 ± 0.025

4.875 ± 0.036

6.466 ± 0.038

5.867 ± 0.025

4.507 ± 0.025

6.914 ± 0.032

1.42 ± 0.015

2.513 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski