Streptomyces wuyuanensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7164 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G9UBM3|A0A1G9UBM3_9ACTN Transport permease protein OS=Streptomyces wuyuanensis OX=1196353 GN=SAMN05444921_11059 PE=3 SV=1
MM1 pKa = 8.01RR2 pKa = 11.84FRR4 pKa = 11.84RR5 pKa = 11.84MGSWAGRR12 pKa = 11.84LGLVVAAGLLGAGVMAPAATAAPAGPQPAVSAAQAAAHH50 pKa = 6.53LPAGDD55 pKa = 3.46GGGPFDD61 pKa = 5.41VEE63 pKa = 5.26LGDD66 pKa = 3.81EE67 pKa = 4.65GNVQVGDD74 pKa = 3.74RR75 pKa = 11.84ACVGNCNNTANGAEE89 pKa = 4.32GEE91 pKa = 4.48DD92 pKa = 3.67GGFCAGLCDD101 pKa = 4.66GSANGGNGSTGEE113 pKa = 3.95AGQEE117 pKa = 4.13GGDD120 pKa = 3.78GGDD123 pKa = 3.59GGICAGICHH132 pKa = 6.64GSVTGGDD139 pKa = 3.41GGGGGDD145 pKa = 4.03AAGEE149 pKa = 4.3GDD151 pKa = 4.36GGTGGAGGDD160 pKa = 3.49GGICIGIGCDD170 pKa = 2.82ISGAGGRR177 pKa = 11.84GGDD180 pKa = 3.6GGEE183 pKa = 4.21GG184 pKa = 3.21

Molecular weight: 16.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9V3B4|A0A1G9V3B4_9ACTN FMN-dependent dehydrogenase includes L-lactate dehydrogenase and type II isopentenyl diphosphate isomerase OS=Streptomyces wuyuanensis OX=1196353 GN=SAMN05444921_111201 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.89GRR40 pKa = 11.84ANLSAA45 pKa = 4.66

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7164

0

7164

2291314

26

6374

319.8

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.754 ± 0.046

0.802 ± 0.007

5.936 ± 0.023

5.842 ± 0.029

2.674 ± 0.017

9.761 ± 0.029

2.281 ± 0.016

3.057 ± 0.019

2.099 ± 0.023

10.149 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.732 ± 0.013

1.713 ± 0.016

6.254 ± 0.032

2.599 ± 0.018

8.363 ± 0.029

5.024 ± 0.019

5.999 ± 0.028

8.477 ± 0.027

1.485 ± 0.014

1.998 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski