Parasedimentitalea marina
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4642 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3T0N3V4|A0A3T0N3V4_9RHOB MerR family transcriptional regulator OS=Parasedimentitalea marina OX=2483033 GN=EBB79_12775 PE=4 SV=1
MM1 pKa = 7.07 ATYY4 pKa = 10.28 VPHH7 pKa = 6.96 ASYY10 pKa = 11.07 RR11 pKa = 11.84 LYY13 pKa = 11.18 DD14 pKa = 3.36 FAGRR18 pKa = 11.84 LFAAEE23 pKa = 4.25 EE24 pKa = 4.28 VEE26 pKa = 4.41 ITQTSNGVDD35 pKa = 3.12 IQFLGFDD42 pKa = 3.32 RR43 pKa = 11.84 RR44 pKa = 11.84 TGEE47 pKa = 4.17 VKK49 pKa = 10.73 VKK51 pKa = 10.8 GGMRR55 pKa = 11.84 LSGDD59 pKa = 3.38 NLVVDD64 pKa = 3.81 NGEE67 pKa = 4.16 IVSGSIGTVQFFAFEE82 pKa = 3.89 FEE84 pKa = 4.72 YY85 pKa = 11.0 YY86 pKa = 10.46 AGRR89 pKa = 11.84 LEE91 pKa = 4.01 SLNWDD96 pKa = 3.33 GTYY99 pKa = 10.55 LQNFLSEE106 pKa = 4.34 TTDD109 pKa = 3.45 GNFSPFYY116 pKa = 10.38 DD117 pKa = 3.86 ALTGAVDD124 pKa = 3.99 LVRR127 pKa = 11.84 GISSDD132 pKa = 3.71 DD133 pKa = 3.95 NYY135 pKa = 11.75 DD136 pKa = 3.1 HH137 pKa = 7.45 SEE139 pKa = 4.41 YY140 pKa = 11.32 YY141 pKa = 10.54 DD142 pKa = 3.3 ATDD145 pKa = 2.83 IHH147 pKa = 7.79 LGAGNDD153 pKa = 3.96 SYY155 pKa = 10.72 THH157 pKa = 6.45 PRR159 pKa = 11.84 LTEE162 pKa = 3.84 MSPLIIDD169 pKa = 3.83 GGEE172 pKa = 3.93 GHH174 pKa = 7.62 DD175 pKa = 4.09 NFQAGIFGSYY185 pKa = 10.11 EE186 pKa = 3.67 PLLIDD191 pKa = 3.26 VAKK194 pKa = 10.8 NLFVDD199 pKa = 4.73 EE200 pKa = 4.72 YY201 pKa = 10.83 GVEE204 pKa = 3.9 HH205 pKa = 6.11 VVRR208 pKa = 11.84 NFEE211 pKa = 4.15 FFQGGRR217 pKa = 11.84 GNDD220 pKa = 3.7 TLIGAMDD227 pKa = 4.88 RR228 pKa = 11.84 PNQFYY233 pKa = 10.32 GYY235 pKa = 9.5 QGSDD239 pKa = 3.36 HH240 pKa = 6.97 ISGGNHH246 pKa = 7.23 DD247 pKa = 5.54 DD248 pKa = 3.8 YY249 pKa = 11.72 LAGGSGLDD257 pKa = 3.48 TILAYY262 pKa = 10.42 GGDD265 pKa = 4.27 DD266 pKa = 4.0 YY267 pKa = 11.7 ILGSSHH273 pKa = 7.67 DD274 pKa = 4.18 YY275 pKa = 11.34 VDD277 pKa = 4.16 GGDD280 pKa = 4.27 GIDD283 pKa = 3.28 TFGYY287 pKa = 10.24 RR288 pKa = 11.84 LIAGYY293 pKa = 10.51 VYY295 pKa = 10.36 IDD297 pKa = 5.36 LEE299 pKa = 4.18 NQEE302 pKa = 4.92 DD303 pKa = 3.84 NRR305 pKa = 11.84 GDD307 pKa = 3.79 AIYY310 pKa = 9.49 TVLVNIEE317 pKa = 4.17 NLNGSNYY324 pKa = 9.03 YY325 pKa = 10.98 DD326 pKa = 4.36 VMLGDD331 pKa = 4.59 ANDD334 pKa = 3.71 NVIEE338 pKa = 4.29 TFGYY342 pKa = 10.41 RR343 pKa = 11.84 DD344 pKa = 3.44 ILHH347 pKa = 6.67 GRR349 pKa = 11.84 DD350 pKa = 3.83 GNDD353 pKa = 3.13 TLDD356 pKa = 3.76 AGEE359 pKa = 4.96 GNDD362 pKa = 4.49 DD363 pKa = 3.83 LSGGTGANLLIGGAGADD380 pKa = 3.24 NFIFEE385 pKa = 4.64 TQNATDD391 pKa = 4.44 TIADD395 pKa = 3.86 FEE397 pKa = 4.69 VGIDD401 pKa = 3.66 SLDD404 pKa = 3.74 FSALTSQADD413 pKa = 4.31 FINFEE418 pKa = 4.81 LNGMSYY424 pKa = 11.01 SRR426 pKa = 11.84 ISCFGDD432 pKa = 3.78 FKK434 pKa = 11.09 LGNVSLRR441 pKa = 11.84 FVQSQEE447 pKa = 3.85 DD448 pKa = 3.84 VEE450 pKa = 5.07 IYY452 pKa = 10.7 LDD454 pKa = 3.78 PSLGGEE460 pKa = 4.27 SASPLITLIDD470 pKa = 3.6 IGIHH474 pKa = 6.19 DD475 pKa = 6.15 LIVDD479 pKa = 3.84 DD480 pKa = 6.89 FIFF483 pKa = 4.49
Molecular weight: 52.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.783
IPC_protein 3.821
Toseland 3.592
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.643
Grimsley 3.503
Solomon 3.821
Lehninger 3.77
Nozaki 3.923
DTASelect 4.202
Thurlkill 3.643
EMBOSS 3.77
Sillero 3.948
Patrickios 0.668
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.827
Protein with the highest isoelectric point:
>tr|A0A3T0MYF2|A0A3T0MYF2_9RHOB Uncharacterized protein OS=Parasedimentitalea marina OX=2483033 GN=EBB79_02025 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 AHH37 pKa = 5.39 GRR39 pKa = 11.84 KK40 pKa = 8.9 EE41 pKa = 3.73 LSAA44 pKa = 4.94
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.402
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4642
0
4642
1420733
26
2158
306.1
33.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.253 ± 0.045
0.97 ± 0.012
6.054 ± 0.029
5.55 ± 0.037
3.842 ± 0.024
8.375 ± 0.035
2.115 ± 0.017
5.43 ± 0.023
3.504 ± 0.032
10.14 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.854 ± 0.016
3.007 ± 0.019
4.822 ± 0.028
3.719 ± 0.021
5.949 ± 0.032
5.893 ± 0.032
5.631 ± 0.025
7.182 ± 0.027
1.389 ± 0.015
2.321 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here