Sphingobium fuliginis (strain ATCC 27551)

Taxonomy: cellular organisms; Bacteria; Proteobacteria;

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4174 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q4J008|A0A4Q4J008_SPHSA Uncharacterized protein OS=Sphingobium fuliginis (strain ATCC 27551) OX=336203 GN=EWH10_09210 PE=4 SV=1
MM1 pKa = 7.7TYY3 pKa = 10.26GHH5 pKa = 6.55SVTIDD10 pKa = 3.05GSLNDD15 pKa = 3.83WLATDD20 pKa = 5.75RR21 pKa = 11.84IDD23 pKa = 5.19DD24 pKa = 3.87NSAPGYY30 pKa = 9.28QVYY33 pKa = 7.91ATLDD37 pKa = 3.03NDD39 pKa = 3.86AFVFALTAPVAIGADD54 pKa = 3.38TTVWLNTDD62 pKa = 2.46GRR64 pKa = 11.84TATGYY69 pKa = 10.64QIFGFAGGAEE79 pKa = 4.26YY80 pKa = 10.84NVNFAADD87 pKa = 3.85GTVNLYY93 pKa = 9.52TGAAGEE99 pKa = 4.22TLVLSGLQTAWSADD113 pKa = 3.3RR114 pKa = 11.84TTVEE118 pKa = 3.96FRR120 pKa = 11.84LPTSAVGNPAAMYY133 pKa = 8.95TYY135 pKa = 11.26YY136 pKa = 10.87DD137 pKa = 3.73INNLSNSFMPSNYY150 pKa = 9.5LSAPFTVFNQTIATQAPDD168 pKa = 3.4MRR170 pKa = 11.84IGIVFSEE177 pKa = 4.71TTADD181 pKa = 3.58AYY183 pKa = 10.61FSPTAYY189 pKa = 9.99SQLFMSVQAQAIQAGIPFDD208 pKa = 3.74ILTEE212 pKa = 4.27ADD214 pKa = 3.58LTDD217 pKa = 4.0LAKK220 pKa = 10.68LSHH223 pKa = 6.55YY224 pKa = 9.76DD225 pKa = 3.34ALVFPSFRR233 pKa = 11.84NVPVDD238 pKa = 3.22KK239 pKa = 11.29VEE241 pKa = 5.14AISHH245 pKa = 4.93TLEE248 pKa = 4.06MASKK252 pKa = 9.88QLGIGLVTAGEE263 pKa = 4.22FMTNQPHH270 pKa = 5.16VQGGTIGGDD279 pKa = 3.71PLPGDD284 pKa = 4.91PYY286 pKa = 11.72AQMKK290 pKa = 10.45LLFDD294 pKa = 3.87ATRR297 pKa = 11.84VTGGTGNVVVNATDD311 pKa = 3.3ATGLVLDD318 pKa = 5.37HH319 pKa = 6.53YY320 pKa = 11.91ANGQQVNSYY329 pKa = 9.56TGVGWNAFTSVSGTGHH345 pKa = 6.43QIATEE350 pKa = 4.33TIDD353 pKa = 3.51GGSVYY358 pKa = 10.57SAALATQTGAARR370 pKa = 11.84NILFSSEE377 pKa = 4.21GIMADD382 pKa = 4.26ANMLQQAISYY392 pKa = 8.11AANGTGLSVGLHH404 pKa = 6.55LSRR407 pKa = 11.84DD408 pKa = 3.67SGIVAARR415 pKa = 11.84VDD417 pKa = 3.54MDD419 pKa = 3.35QSQEE423 pKa = 4.1LEE425 pKa = 4.24DD426 pKa = 3.92VNPAGSTPGIYY437 pKa = 10.22DD438 pKa = 3.48KK439 pKa = 11.03LLPILTQWKK448 pKa = 9.71ADD450 pKa = 3.66YY451 pKa = 10.88NFVGSFYY458 pKa = 11.08LNVGDD463 pKa = 4.17GTNGTGTDD471 pKa = 3.38WAVSLPYY478 pKa = 10.58YY479 pKa = 10.32KK480 pKa = 10.85ALLDD484 pKa = 4.16LGNEE488 pKa = 4.13IGTHH492 pKa = 5.2SLTHH496 pKa = 6.61PEE498 pKa = 3.9NTNLLSAQQIQTEE511 pKa = 4.4FQNSITILNQQIGAYY526 pKa = 9.79LNQPFQITGAAVPGAPEE543 pKa = 4.61LLPTSQEE550 pKa = 3.34IMKK553 pKa = 10.26YY554 pKa = 10.09VSDD557 pKa = 4.24YY558 pKa = 10.76LTGGYY563 pKa = 9.32AAQGAGYY570 pKa = 8.77PNAFGYY576 pKa = 7.39LTPDD580 pKa = 3.36NQNQIYY586 pKa = 8.43FAPNTSFDD594 pKa = 3.43YY595 pKa = 11.04TLIEE599 pKa = 4.29FQKK602 pKa = 9.69KK603 pKa = 6.02TVAEE607 pKa = 4.36ATAAWIGEE615 pKa = 3.86FDD617 pKa = 3.91KK618 pKa = 11.41LVANAQTPVIVWPWHH633 pKa = 6.94DD634 pKa = 3.57YY635 pKa = 10.36GAADD639 pKa = 4.58FLSGSPTVDD648 pKa = 2.85SPYY651 pKa = 9.97DD652 pKa = 3.24ISMFTSWLQHH662 pKa = 5.02AVAANMEE669 pKa = 4.58FVTLDD674 pKa = 3.39DD675 pKa = 3.79LAARR679 pKa = 11.84MTAVRR684 pKa = 11.84NATITTTVAGNSITASVGGANVGNLSLDD712 pKa = 3.38VDD714 pKa = 3.89RR715 pKa = 11.84QGALVIQNVAGWYY728 pKa = 10.1AYY730 pKa = 10.56DD731 pKa = 3.7SDD733 pKa = 5.55SVFLPQSGGTFRR745 pKa = 11.84INLGAAADD753 pKa = 4.23DD754 pKa = 3.97VTHH757 pKa = 6.31ITDD760 pKa = 3.66LPMRR764 pKa = 11.84AVLLSVAGDD773 pKa = 3.42GHH775 pKa = 6.48NLAFSVQGEE784 pKa = 4.55GKK786 pKa = 10.33VVIDD790 pKa = 3.9IANPGTDD797 pKa = 3.75GIVVTGALGYY807 pKa = 10.36SRR809 pKa = 11.84VGEE812 pKa = 4.21IVTVDD817 pKa = 3.19LGGYY821 pKa = 8.54GLHH824 pKa = 6.56NVTLEE829 pKa = 4.09YY830 pKa = 10.76KK831 pKa = 9.24NPPVIVSGGGGPTAALSMAEE851 pKa = 4.21NGTAVTTVVATDD863 pKa = 3.59ADD865 pKa = 3.83VGTVIGYY872 pKa = 9.33SISGGADD879 pKa = 2.89AAKK882 pKa = 10.38FKK884 pKa = 10.55IDD886 pKa = 3.4SATGALAFLTAPNFEE901 pKa = 4.47AATDD905 pKa = 3.45AGTNNVYY912 pKa = 10.74DD913 pKa = 3.91VVVRR917 pKa = 11.84ASDD920 pKa = 3.32GTLYY924 pKa = 10.68DD925 pKa = 3.86DD926 pKa = 3.62QAIAVTITNVNEE938 pKa = 4.03APVITSNGGGASAAISVAEE957 pKa = 4.14NTTAVTSVTATDD969 pKa = 3.67PDD971 pKa = 3.95AATVLAYY978 pKa = 10.57SIVGGPDD985 pKa = 2.74RR986 pKa = 11.84ALFSIDD992 pKa = 3.3ASTGALAFLSPPNYY1006 pKa = 10.02DD1007 pKa = 3.88LPGDD1011 pKa = 3.82ANANNVYY1018 pKa = 10.36SLTVRR1023 pKa = 11.84VSDD1026 pKa = 3.91GALFDD1031 pKa = 4.32DD1032 pKa = 3.96QAINVTVTNANEE1044 pKa = 3.95HH1045 pKa = 6.3APVITSGGGGDD1056 pKa = 3.49TAAVSVSTGTRR1067 pKa = 11.84PVMKK1071 pKa = 9.45VTAVDD1076 pKa = 3.51PDD1078 pKa = 4.0ANTTLAYY1085 pKa = 10.36SIAGGVDD1092 pKa = 3.0ASKK1095 pKa = 9.33FTINASTGQLNFVNTPNYY1113 pKa = 10.29LLPTDD1118 pKa = 3.61TGKK1121 pKa = 10.79DD1122 pKa = 3.09NVYY1125 pKa = 10.87DD1126 pKa = 4.14VIVRR1130 pKa = 11.84ASDD1133 pKa = 3.14GTFYY1137 pKa = 11.0DD1138 pKa = 3.91DD1139 pKa = 3.15QAIAVTVTILNAAPIIISNGGGASATIRR1167 pKa = 11.84MAEE1170 pKa = 4.19NGTAVTKK1177 pKa = 10.5VVATDD1182 pKa = 3.32VNLGQTITYY1191 pKa = 9.84SLGSAVDD1198 pKa = 3.7GALFSIDD1205 pKa = 3.67PQTGVLTFKK1214 pKa = 10.48SAPDD1218 pKa = 3.74FEE1220 pKa = 6.3APADD1224 pKa = 3.87SNLDD1228 pKa = 2.83NRR1230 pKa = 11.84YY1231 pKa = 8.85QVQVLATDD1239 pKa = 3.44SLGAQDD1245 pKa = 4.31FQNLTVVVNNVSGLSLTAASGGSTLIGQGEE1275 pKa = 4.67EE1276 pKa = 3.94DD1277 pKa = 3.82TLRR1280 pKa = 11.84GAGGNDD1286 pKa = 3.26TLIGNGGSDD1295 pKa = 3.73TLNGAGGNDD1304 pKa = 4.02IIDD1307 pKa = 4.15GGAGADD1313 pKa = 4.32RR1314 pKa = 11.84ITGGAGADD1322 pKa = 3.5ILTGGADD1329 pKa = 3.7ADD1331 pKa = 4.0LFIYY1335 pKa = 10.42NATGNSTPAIMDD1347 pKa = 4.84RR1348 pKa = 11.84IMDD1351 pKa = 4.18FQQGIDD1357 pKa = 4.27RR1358 pKa = 11.84IDD1360 pKa = 3.74LSAIDD1365 pKa = 4.86AMPALSGNQAFSFIGNAAFSGPGQLHH1391 pKa = 6.24VYY1393 pKa = 10.92SNGTDD1398 pKa = 3.18SFVEE1402 pKa = 4.04ANVDD1406 pKa = 3.7NNPATVDD1413 pKa = 3.7FAIRR1417 pKa = 11.84LIGVHH1422 pKa = 6.35NLTASDD1428 pKa = 3.96FLLL1431 pKa = 4.74

Molecular weight: 148.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A292ZAA8|A0A292ZAA8_SPHSA UPF0235 protein EWH10_07690 OS=Sphingobium fuliginis (strain ATCC 27551) OX=336203 GN=EWH10_07690 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4174

0

4174

1366607

28

3242

327.4

35.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.183 ± 0.052

0.791 ± 0.01

5.971 ± 0.03

5.375 ± 0.037

3.538 ± 0.024

9.016 ± 0.04

2.061 ± 0.021

5.002 ± 0.023

2.88 ± 0.028

10.04 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.554 ± 0.021

2.549 ± 0.026

5.342 ± 0.027

3.208 ± 0.023

7.576 ± 0.041

5.306 ± 0.025

4.96 ± 0.038

6.989 ± 0.031

1.433 ± 0.018

2.227 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski