Serratia phage Moabite
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 338 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y5TQL5|A0A4Y5TQL5_9CAUD Uncharacterized protein OS=Serratia phage Moabite OX=2587814 GN=CPT_Moabite_243 PE=4 SV=1
MM1 pKa = 7.18 YY2 pKa = 10.14 IKK4 pKa = 10.59 GFFPITVNDD13 pKa = 4.09 DD14 pKa = 3.27 KK15 pKa = 11.93 GDD17 pKa = 4.04 VIKK20 pKa = 9.93 LTYY23 pKa = 10.61 DD24 pKa = 3.34 EE25 pKa = 5.01 AVCDD29 pKa = 4.76 IISADD34 pKa = 3.68 PLGEE38 pKa = 4.98 DD39 pKa = 4.57 IYY41 pKa = 11.38 ATLCIYY47 pKa = 11.04 SDD49 pKa = 3.8 VTRR52 pKa = 11.84 EE53 pKa = 3.91 TPVFNFTATVSAAGVTDD70 pKa = 3.57 QNIAEE75 pKa = 4.18 LKK77 pKa = 10.35 IFEE80 pKa = 4.13 EE81 pKa = 5.12 AINNVCHH88 pKa = 6.48 GFTYY92 pKa = 10.9 VKK94 pKa = 10.63 DD95 pKa = 3.52
Molecular weight: 10.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 4.024
IPC_protein 3.961
Toseland 3.757
ProMoST 4.05
Dawson 3.948
Bjellqvist 4.164
Wikipedia 3.897
Rodwell 3.783
Grimsley 3.668
Solomon 3.935
Lehninger 3.897
Nozaki 4.075
DTASelect 4.291
Thurlkill 3.808
EMBOSS 3.897
Sillero 4.075
Patrickios 0.896
IPC_peptide 3.935
IPC2_peptide 4.05
IPC2.peptide.svr19 4.018
Protein with the highest isoelectric point:
>tr|A0A4Y5TPH3|A0A4Y5TPH3_9CAUD Uncharacterized protein OS=Serratia phage Moabite OX=2587814 GN=CPT_Moabite_238 PE=4 SV=1
MM1 pKa = 7.7 KK2 pKa = 9.95 PGNLGKK8 pKa = 8.7 TPVTGTHH15 pKa = 6.78 GYY17 pKa = 9.87 VVDD20 pKa = 3.85 SSFYY24 pKa = 10.33 PSNQPCVRR32 pKa = 11.84 RR33 pKa = 11.84 KK34 pKa = 8.07 VTMKK38 pKa = 10.13 RR39 pKa = 11.84 SRR41 pKa = 11.84 VATAVGMEE49 pKa = 3.8 VWVFGRR55 pKa = 11.84 GIQQ58 pKa = 3.75
Molecular weight: 6.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.926
IPC_protein 10.672
Toseland 10.818
ProMoST 10.496
Dawson 10.921
Bjellqvist 10.613
Wikipedia 11.111
Rodwell 11.228
Grimsley 10.965
Solomon 11.023
Lehninger 10.994
Nozaki 10.804
DTASelect 10.613
Thurlkill 10.818
EMBOSS 11.213
Sillero 10.847
Patrickios 11.023
IPC_peptide 11.038
IPC2_peptide 9.619
IPC2.peptide.svr19 8.326
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
338
0
338
85942
30
2213
254.3
28.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.052 ± 0.158
0.918 ± 0.053
6.333 ± 0.094
6.362 ± 0.176
4.355 ± 0.087
6.684 ± 0.185
1.634 ± 0.065
6.445 ± 0.111
5.905 ± 0.114
8.275 ± 0.119
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.69 ± 0.071
5.479 ± 0.119
4.024 ± 0.081
3.065 ± 0.093
5.443 ± 0.12
6.543 ± 0.099
6.294 ± 0.099
7.226 ± 0.121
1.283 ± 0.054
3.989 ± 0.095
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here