Amino acid dipepetide frequency for Serratia phage Moabite

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.353AlaAla: 6.353 ± 0.428
0.442AlaCys: 0.442 ± 0.072
4.224AlaAsp: 4.224 ± 0.266
4.026AlaGlu: 4.026 ± 0.236
2.909AlaPhe: 2.909 ± 0.181
4.759AlaGly: 4.759 ± 0.292
1.001AlaHis: 1.001 ± 0.103
5.178AlaIle: 5.178 ± 0.221
4.515AlaLys: 4.515 ± 0.246
6.458AlaLeu: 6.458 ± 0.284
2.164AlaMet: 2.164 ± 0.146
4.119AlaAsn: 4.119 ± 0.264
2.572AlaPro: 2.572 ± 0.166
2.071AlaGln: 2.071 ± 0.155
2.944AlaArg: 2.944 ± 0.199
3.689AlaSer: 3.689 ± 0.213
4.794AlaThr: 4.794 ± 0.255
5.213AlaVal: 5.213 ± 0.286
0.826AlaTrp: 0.826 ± 0.121
2.257AlaTyr: 2.257 ± 0.158
0.0AlaXaa: 0.0 ± 0.0
Cys
0.593CysAla: 0.593 ± 0.075
0.116CysCys: 0.116 ± 0.039
0.477CysAsp: 0.477 ± 0.077
0.628CysGlu: 0.628 ± 0.09
0.489CysPhe: 0.489 ± 0.068
0.582CysGly: 0.582 ± 0.088
0.105CysHis: 0.105 ± 0.033
0.57CysIle: 0.57 ± 0.077
0.57CysLys: 0.57 ± 0.075
0.663CysLeu: 0.663 ± 0.089
0.198CysMet: 0.198 ± 0.05
0.617CysAsn: 0.617 ± 0.093
0.361CysPro: 0.361 ± 0.074
0.233CysGln: 0.233 ± 0.044
0.628CysArg: 0.628 ± 0.086
0.593CysSer: 0.593 ± 0.096
0.698CysThr: 0.698 ± 0.098
0.512CysVal: 0.512 ± 0.074
0.116CysTrp: 0.116 ± 0.038
0.431CysTyr: 0.431 ± 0.069
0.0CysXaa: 0.0 ± 0.0
Asp
4.061AspAla: 4.061 ± 0.242
0.547AspCys: 0.547 ± 0.086
3.712AspAsp: 3.712 ± 0.227
3.968AspGlu: 3.968 ± 0.261
2.874AspPhe: 2.874 ± 0.191
4.468AspGly: 4.468 ± 0.251
0.908AspHis: 0.908 ± 0.107
4.375AspIle: 4.375 ± 0.216
3.735AspLys: 3.735 ± 0.236
5.713AspLeu: 5.713 ± 0.232
1.443AspMet: 1.443 ± 0.137
3.491AspAsn: 3.491 ± 0.201
2.862AspPro: 2.862 ± 0.216
2.292AspGln: 2.292 ± 0.147
3.223AspArg: 3.223 ± 0.237
3.712AspSer: 3.712 ± 0.222
3.549AspThr: 3.549 ± 0.182
4.422AspVal: 4.422 ± 0.209
0.943AspTrp: 0.943 ± 0.091
3.037AspTyr: 3.037 ± 0.204
0.0AspXaa: 0.0 ± 0.0
Glu
4.619GluAla: 4.619 ± 0.315
0.465GluCys: 0.465 ± 0.07
3.817GluAsp: 3.817 ± 0.227
4.759GluGlu: 4.759 ± 0.274
2.897GluPhe: 2.897 ± 0.189
4.201GluGly: 4.201 ± 0.174
1.257GluHis: 1.257 ± 0.141
4.596GluIle: 4.596 ± 0.234
3.409GluLys: 3.409 ± 0.254
5.667GluLeu: 5.667 ± 0.28
2.071GluMet: 2.071 ± 0.137
2.967GluAsn: 2.967 ± 0.165
1.838GluPro: 1.838 ± 0.144
2.269GluGln: 2.269 ± 0.173
3.305GluArg: 3.305 ± 0.197
3.828GluSer: 3.828 ± 0.226
3.293GluThr: 3.293 ± 0.201
4.887GluVal: 4.887 ± 0.287
0.896GluTrp: 0.896 ± 0.107
2.583GluTyr: 2.583 ± 0.158
0.0GluXaa: 0.0 ± 0.0
Phe
2.793PheAla: 2.793 ± 0.176
0.419PheCys: 0.419 ± 0.063
3.002PheAsp: 3.002 ± 0.206
2.35PheGlu: 2.35 ± 0.17
1.92PhePhe: 1.92 ± 0.155
2.932PheGly: 2.932 ± 0.197
0.838PheHis: 0.838 ± 0.1
3.118PheIle: 3.118 ± 0.192
2.583PheLys: 2.583 ± 0.165
2.897PheLeu: 2.897 ± 0.199
1.129PheMet: 1.129 ± 0.119
2.839PheAsn: 2.839 ± 0.218
1.582PhePro: 1.582 ± 0.137
1.152PheGln: 1.152 ± 0.127
2.257PheArg: 2.257 ± 0.191
3.409PheSer: 3.409 ± 0.192
3.142PheThr: 3.142 ± 0.201
2.932PheVal: 2.932 ± 0.17
0.465PheTrp: 0.465 ± 0.072
1.792PheTyr: 1.792 ± 0.149
0.0PheXaa: 0.0 ± 0.0
Gly
3.933GlyAla: 3.933 ± 0.319
0.849GlyCys: 0.849 ± 0.117
3.933GlyAsp: 3.933 ± 0.26
4.363GlyGlu: 4.363 ± 0.213
3.014GlyPhe: 3.014 ± 0.187
4.201GlyGly: 4.201 ± 0.397
1.001GlyHis: 1.001 ± 0.105
4.701GlyIle: 4.701 ± 0.225
5.085GlyLys: 5.085 ± 0.268
5.015GlyLeu: 5.015 ± 0.236
1.827GlyMet: 1.827 ± 0.155
3.991GlyAsn: 3.991 ± 0.275
1.652GlyPro: 1.652 ± 0.188
1.978GlyGln: 1.978 ± 0.183
3.142GlyArg: 3.142 ± 0.226
4.608GlySer: 4.608 ± 0.247
4.794GlyThr: 4.794 ± 0.291
4.724GlyVal: 4.724 ± 0.229
0.919GlyTrp: 0.919 ± 0.1
3.107GlyTyr: 3.107 ± 0.173
0.0GlyXaa: 0.0 ± 0.0
His
0.861HisAla: 0.861 ± 0.095
0.314HisCys: 0.314 ± 0.059
0.849HisAsp: 0.849 ± 0.103
0.908HisGlu: 0.908 ± 0.118
0.954HisPhe: 0.954 ± 0.106
1.047HisGly: 1.047 ± 0.117
0.419HisHis: 0.419 ± 0.068
0.826HisIle: 0.826 ± 0.111
0.849HisLys: 0.849 ± 0.098
1.571HisLeu: 1.571 ± 0.151
0.431HisMet: 0.431 ± 0.069
0.791HisAsn: 0.791 ± 0.107
0.954HisPro: 0.954 ± 0.114
0.384HisGln: 0.384 ± 0.077
1.001HisArg: 1.001 ± 0.126
0.966HisSer: 0.966 ± 0.096
0.896HisThr: 0.896 ± 0.091
1.012HisVal: 1.012 ± 0.118
0.303HisTrp: 0.303 ± 0.061
1.001HisTyr: 1.001 ± 0.107
0.0HisXaa: 0.0 ± 0.0
Ile
4.608IleAla: 4.608 ± 0.214
0.547IleCys: 0.547 ± 0.075
4.957IleAsp: 4.957 ± 0.202
4.34IleGlu: 4.34 ± 0.201
2.478IlePhe: 2.478 ± 0.181
3.723IleGly: 3.723 ± 0.26
1.117IleHis: 1.117 ± 0.135
3.456IleIle: 3.456 ± 0.195
3.886IleLys: 3.886 ± 0.219
4.689IleLeu: 4.689 ± 0.197
1.408IleMet: 1.408 ± 0.143
4.061IleAsn: 4.061 ± 0.2
2.956IlePro: 2.956 ± 0.198
2.129IleGln: 2.129 ± 0.171
3.991IleArg: 3.991 ± 0.212
4.457IleSer: 4.457 ± 0.239
4.41IleThr: 4.41 ± 0.239
4.247IleVal: 4.247 ± 0.246
0.698IleTrp: 0.698 ± 0.097
2.292IleTyr: 2.292 ± 0.16
0.0IleXaa: 0.0 ± 0.0
Lys
4.235LysAla: 4.235 ± 0.216
0.5LysCys: 0.5 ± 0.078
3.851LysAsp: 3.851 ± 0.215
4.491LysGlu: 4.491 ± 0.257
2.56LysPhe: 2.56 ± 0.184
3.921LysGly: 3.921 ± 0.226
1.117LysHis: 1.117 ± 0.141
3.456LysIle: 3.456 ± 0.208
2.956LysLys: 2.956 ± 0.212
4.91LysLeu: 4.91 ± 0.269
1.85LysMet: 1.85 ± 0.159
2.444LysAsn: 2.444 ± 0.163
2.385LysPro: 2.385 ± 0.135
1.71LysGln: 1.71 ± 0.134
3.293LysArg: 3.293 ± 0.21
3.782LysSer: 3.782 ± 0.206
3.584LysThr: 3.584 ± 0.227
4.887LysVal: 4.887 ± 0.269
0.721LysTrp: 0.721 ± 0.064
2.397LysTyr: 2.397 ± 0.176
0.0LysXaa: 0.0 ± 0.0
Leu
6.097LeuAla: 6.097 ± 0.256
0.71LeuCys: 0.71 ± 0.099
5.155LeuAsp: 5.155 ± 0.249
5.574LeuGlu: 5.574 ± 0.294
3.049LeuPhe: 3.049 ± 0.198
5.225LeuGly: 5.225 ± 0.269
1.268LeuHis: 1.268 ± 0.12
5.073LeuIle: 5.073 ± 0.238
5.225LeuLys: 5.225 ± 0.306
6.365LeuLeu: 6.365 ± 0.341
2.211LeuMet: 2.211 ± 0.169
5.085LeuAsn: 5.085 ± 0.262
3.817LeuPro: 3.817 ± 0.215
2.316LeuGln: 2.316 ± 0.169
4.841LeuArg: 4.841 ± 0.232
6.283LeuSer: 6.283 ± 0.287
5.329LeuThr: 5.329 ± 0.282
5.457LeuVal: 5.457 ± 0.225
0.861LeuTrp: 0.861 ± 0.125
2.816LeuTyr: 2.816 ± 0.198
0.0LeuXaa: 0.0 ± 0.0
Met
2.211MetAla: 2.211 ± 0.172
0.256MetCys: 0.256 ± 0.052
1.757MetAsp: 1.757 ± 0.147
1.699MetGlu: 1.699 ± 0.15
1.082MetPhe: 1.082 ± 0.127
1.734MetGly: 1.734 ± 0.131
0.396MetHis: 0.396 ± 0.072
1.71MetIle: 1.71 ± 0.145
1.769MetLys: 1.769 ± 0.149
2.001MetLeu: 2.001 ± 0.168
0.652MetMet: 0.652 ± 0.095
1.524MetAsn: 1.524 ± 0.146
0.908MetPro: 0.908 ± 0.107
0.5MetGln: 0.5 ± 0.081
1.385MetArg: 1.385 ± 0.124
2.374MetSer: 2.374 ± 0.138
1.71MetThr: 1.71 ± 0.138
2.141MetVal: 2.141 ± 0.162
0.221MetTrp: 0.221 ± 0.058
0.873MetTyr: 0.873 ± 0.11
0.0MetXaa: 0.0 ± 0.0
Asn
3.84AsnAla: 3.84 ± 0.215
0.524AsnCys: 0.524 ± 0.097
3.689AsnAsp: 3.689 ± 0.233
3.456AsnGlu: 3.456 ± 0.238
2.502AsnPhe: 2.502 ± 0.196
4.654AsnGly: 4.654 ± 0.262
0.663AsnHis: 0.663 ± 0.084
2.979AsnIle: 2.979 ± 0.19
2.874AsnLys: 2.874 ± 0.18
4.573AsnLeu: 4.573 ± 0.25
1.606AsnMet: 1.606 ± 0.129
2.723AsnAsn: 2.723 ± 0.278
3.13AsnPro: 3.13 ± 0.203
1.862AsnGln: 1.862 ± 0.164
3.212AsnArg: 3.212 ± 0.214
3.34AsnSer: 3.34 ± 0.218
2.967AsnThr: 2.967 ± 0.161
3.514AsnVal: 3.514 ± 0.189
0.628AsnTrp: 0.628 ± 0.089
2.06AsnTyr: 2.06 ± 0.17
0.0AsnXaa: 0.0 ± 0.0
Pro
2.665ProAla: 2.665 ± 0.169
0.337ProCys: 0.337 ± 0.059
3.037ProAsp: 3.037 ± 0.18
3.305ProGlu: 3.305 ± 0.221
1.745ProPhe: 1.745 ± 0.136
2.967ProGly: 2.967 ± 0.184
0.652ProHis: 0.652 ± 0.119
2.42ProIle: 2.42 ± 0.187
2.234ProLys: 2.234 ± 0.165
2.886ProLeu: 2.886 ± 0.176
0.943ProMet: 0.943 ± 0.105
2.222ProAsn: 2.222 ± 0.16
1.222ProPro: 1.222 ± 0.136
0.954ProGln: 0.954 ± 0.095
1.792ProArg: 1.792 ± 0.141
2.502ProSer: 2.502 ± 0.166
2.932ProThr: 2.932 ± 0.197
3.514ProVal: 3.514 ± 0.242
0.5ProTrp: 0.5 ± 0.085
1.408ProTyr: 1.408 ± 0.134
0.0ProXaa: 0.0 ± 0.0
Gln
2.292GlnAla: 2.292 ± 0.166
0.291GlnCys: 0.291 ± 0.052
1.466GlnAsp: 1.466 ± 0.117
1.652GlnGlu: 1.652 ± 0.149
1.373GlnPhe: 1.373 ± 0.098
1.885GlnGly: 1.885 ± 0.174
0.431GlnHis: 0.431 ± 0.07
2.118GlnIle: 2.118 ± 0.156
1.338GlnLys: 1.338 ± 0.123
2.769GlnLeu: 2.769 ± 0.176
0.954GlnMet: 0.954 ± 0.101
1.338GlnAsn: 1.338 ± 0.145
1.222GlnPro: 1.222 ± 0.111
1.117GlnGln: 1.117 ± 0.14
2.153GlnArg: 2.153 ± 0.166
2.013GlnSer: 2.013 ± 0.144
1.897GlnThr: 1.897 ± 0.123
2.374GlnVal: 2.374 ± 0.173
0.559GlnTrp: 0.559 ± 0.081
1.408GlnTyr: 1.408 ± 0.127
0.0GlnXaa: 0.0 ± 0.0
Arg
3.467ArgAla: 3.467 ± 0.226
0.489ArgCys: 0.489 ± 0.072
3.177ArgAsp: 3.177 ± 0.212
3.246ArgGlu: 3.246 ± 0.217
2.502ArgPhe: 2.502 ± 0.139
3.63ArgGly: 3.63 ± 0.228
1.024ArgHis: 1.024 ± 0.115
3.595ArgIle: 3.595 ± 0.216
3.153ArgLys: 3.153 ± 0.215
4.561ArgLeu: 4.561 ± 0.25
1.326ArgMet: 1.326 ± 0.158
3.281ArgAsn: 3.281 ± 0.176
1.978ArgPro: 1.978 ± 0.146
1.908ArgGln: 1.908 ± 0.145
3.305ArgArg: 3.305 ± 0.24
3.386ArgSer: 3.386 ± 0.222
3.235ArgThr: 3.235 ± 0.192
3.886ArgVal: 3.886 ± 0.205
0.803ArgTrp: 0.803 ± 0.092
2.478ArgTyr: 2.478 ± 0.148
0.0ArgXaa: 0.0 ± 0.0
Ser
5.062SerAla: 5.062 ± 0.226
0.617SerCys: 0.617 ± 0.092
4.096SerAsp: 4.096 ± 0.204
3.817SerGlu: 3.817 ± 0.205
2.874SerPhe: 2.874 ± 0.173
4.596SerGly: 4.596 ± 0.29
1.047SerHis: 1.047 ± 0.118
4.329SerIle: 4.329 ± 0.23
4.061SerLys: 4.061 ± 0.21
5.539SerLeu: 5.539 ± 0.244
1.815SerMet: 1.815 ± 0.156
3.502SerAsn: 3.502 ± 0.208
2.572SerPro: 2.572 ± 0.2
1.757SerGln: 1.757 ± 0.15
3.316SerArg: 3.316 ± 0.216
4.212SerSer: 4.212 ± 0.232
4.259SerThr: 4.259 ± 0.225
4.759SerVal: 4.759 ± 0.267
0.873SerTrp: 0.873 ± 0.099
2.327SerTyr: 2.327 ± 0.196
0.0SerXaa: 0.0 ± 0.0
Thr
4.491ThrAla: 4.491 ± 0.221
0.547ThrCys: 0.547 ± 0.091
4.003ThrAsp: 4.003 ± 0.195
3.607ThrGlu: 3.607 ± 0.264
2.99ThrPhe: 2.99 ± 0.186
4.631ThrGly: 4.631 ± 0.315
1.047ThrHis: 1.047 ± 0.106
4.154ThrIle: 4.154 ± 0.222
3.782ThrLys: 3.782 ± 0.186
5.946ThrLeu: 5.946 ± 0.225
1.443ThrMet: 1.443 ± 0.119
2.99ThrAsn: 2.99 ± 0.2
3.107ThrPro: 3.107 ± 0.166
1.92ThrGln: 1.92 ± 0.136
3.072ThrArg: 3.072 ± 0.2
3.665ThrSer: 3.665 ± 0.208
3.654ThrThr: 3.654 ± 0.231
4.585ThrVal: 4.585 ± 0.221
0.943ThrTrp: 0.943 ± 0.111
2.362ThrTyr: 2.362 ± 0.168
0.0ThrXaa: 0.0 ± 0.0
Val
4.91ValAla: 4.91 ± 0.266
0.559ValCys: 0.559 ± 0.089
4.736ValAsp: 4.736 ± 0.211
4.747ValGlu: 4.747 ± 0.253
2.921ValPhe: 2.921 ± 0.179
4.247ValGly: 4.247 ± 0.245
1.082ValHis: 1.082 ± 0.126
4.945ValIle: 4.945 ± 0.254
4.608ValLys: 4.608 ± 0.243
5.446ValLeu: 5.446 ± 0.247
1.815ValMet: 1.815 ± 0.122
4.294ValAsn: 4.294 ± 0.256
3.095ValPro: 3.095 ± 0.193
2.164ValGln: 2.164 ± 0.18
3.735ValArg: 3.735 ± 0.176
5.085ValSer: 5.085 ± 0.216
4.957ValThr: 4.957 ± 0.249
5.469ValVal: 5.469 ± 0.255
0.721ValTrp: 0.721 ± 0.094
2.723ValTyr: 2.723 ± 0.17
0.0ValXaa: 0.0 ± 0.0
Trp
0.943TrpAla: 0.943 ± 0.109
0.198TrpCys: 0.198 ± 0.047
0.652TrpAsp: 0.652 ± 0.09
0.745TrpGlu: 0.745 ± 0.097
0.617TrpPhe: 0.617 ± 0.083
0.745TrpGly: 0.745 ± 0.101
0.233TrpHis: 0.233 ± 0.056
0.849TrpIle: 0.849 ± 0.102
0.663TrpLys: 0.663 ± 0.082
1.385TrpLeu: 1.385 ± 0.131
0.489TrpMet: 0.489 ± 0.08
0.489TrpAsn: 0.489 ± 0.077
0.268TrpPro: 0.268 ± 0.05
0.372TrpGln: 0.372 ± 0.07
0.931TrpArg: 0.931 ± 0.102
0.756TrpSer: 0.756 ± 0.103
0.698TrpThr: 0.698 ± 0.099
1.094TrpVal: 1.094 ± 0.111
0.256TrpTrp: 0.256 ± 0.054
0.454TrpTyr: 0.454 ± 0.064
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.49TyrAla: 2.49 ± 0.174
0.454TyrCys: 0.454 ± 0.087
2.746TyrAsp: 2.746 ± 0.188
1.943TyrGlu: 1.943 ± 0.151
1.804TyrPhe: 1.804 ± 0.133
2.688TyrGly: 2.688 ± 0.172
0.733TyrHis: 0.733 ± 0.101
2.001TyrIle: 2.001 ± 0.151
1.757TyrLys: 1.757 ± 0.153
3.7TyrLeu: 3.7 ± 0.232
1.117TyrMet: 1.117 ± 0.115
2.025TyrAsn: 2.025 ± 0.153
1.757TyrPro: 1.757 ± 0.15
1.559TyrGln: 1.559 ± 0.116
2.909TyrArg: 2.909 ± 0.202
2.769TyrSer: 2.769 ± 0.166
2.141TyrThr: 2.141 ± 0.166
2.63TyrVal: 2.63 ± 0.176
0.582TyrTrp: 0.582 ± 0.093
2.083TyrTyr: 2.083 ± 0.167
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 338 proteins (85942 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski