Collinsella sp. BA40
Average proteome isoelectric point is 5.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1521 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C7EYM6|A0A5C7EYM6_9ACTN TetM/TetW/TetO/TetS family tetracycline resistance ribosomal protection protein OS=Collinsella sp. BA40 OX=2560852 GN=E4J93_05930 PE=3 SV=1
MM1 pKa = 7.6 LEE3 pKa = 4.16 GATPYY8 pKa = 10.4 HH9 pKa = 6.41 WVAHH13 pKa = 6.99 DD14 pKa = 3.63 GTQIFAAYY22 pKa = 9.26 IRR24 pKa = 11.84 DD25 pKa = 3.49 GKK27 pKa = 9.46 VIKK30 pKa = 9.97 VAKK33 pKa = 9.73 FYY35 pKa = 11.39 VGTDD39 pKa = 3.09 YY40 pKa = 11.3 WNAADD45 pKa = 5.53 GSDD48 pKa = 4.07 LGKK51 pKa = 10.06 PNLDD55 pKa = 3.45 GTGTQIDD62 pKa = 3.91 NRR64 pKa = 11.84 LPPQLVDD71 pKa = 5.4 PEE73 pKa = 4.86 DD74 pKa = 3.74 FDD76 pKa = 4.58 NAEE79 pKa = 4.63 DD80 pKa = 4.12 YY81 pKa = 11.65 ADD83 pKa = 3.62 NAADD87 pKa = 3.5 YY88 pKa = 10.9 FEE90 pKa = 5.41 YY91 pKa = 11.04 YY92 pKa = 9.46 GAKK95 pKa = 9.53 DD96 pKa = 3.24 PWMAAVDD103 pKa = 4.34 FWDD106 pKa = 4.23 LNGPP110 pKa = 3.87
Molecular weight: 12.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.884
IPC_protein 3.884
Toseland 3.656
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.872
Rodwell 3.706
Grimsley 3.567
Solomon 3.884
Lehninger 3.846
Nozaki 4.024
DTASelect 4.317
Thurlkill 3.732
EMBOSS 3.884
Sillero 4.012
Patrickios 1.939
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.899
Protein with the highest isoelectric point:
>tr|A0A5C7F272|A0A5C7F272_9ACTN Asparagine synthetase domain-containing protein OS=Collinsella sp. BA40 OX=2560852 GN=E4J93_01220 PE=4 SV=1
MM1 pKa = 7.71 RR2 pKa = 11.84 PSPLPPSPPPPPRR15 pKa = 11.84 PLRR18 pKa = 11.84 PAPPPPPRR26 pKa = 11.84 PPRR29 pKa = 11.84 PSPLL33 pKa = 3.33
Molecular weight: 3.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.386
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.901
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.16
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1521
0
1521
543261
33
3499
357.2
38.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.174 ± 0.103
1.493 ± 0.03
6.481 ± 0.045
6.602 ± 0.053
3.589 ± 0.036
8.253 ± 0.057
1.914 ± 0.026
4.974 ± 0.051
3.496 ± 0.062
9.119 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.66 ± 0.033
2.746 ± 0.043
4.358 ± 0.041
2.797 ± 0.031
6.474 ± 0.069
5.834 ± 0.049
5.196 ± 0.048
8.034 ± 0.055
1.031 ± 0.021
2.773 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here