Pseudogymnoascus destructans (strain ATCC MYA-4855 / 20631-21) (Bat white-nose syndrome fungus) (Geomyces destructans)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus; Pseudogymnoascus destructans

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9122 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L8FSY3|L8FSY3_PSED2 FSH1 domain-containing protein OS=Pseudogymnoascus destructans (strain ATCC MYA-4855 / 20631-21) OX=658429 GN=GMDG_06307 PE=4 SV=1
EEE2 pKa = 4.01SGDDD6 pKa = 3.84IPGYYY11 pKa = 9.18TAPSDDD17 pKa = 4.02SNLFGTDDD25 pKa = 3.36DD26 pKa = 3.92ALNSAGGNAVGNEEE40 pKa = 3.49WEEE43 pKa = 4.36MMGADDD49 pKa = 4.07NRR51 pKa = 11.84ATVDDD56 pKa = 3.4SSFEEE61 pKa = 5.25DD62 pKa = 3.5IKKK65 pKa = 10.53KK66 pKa = 10.56EEE68 pKa = 4.26EE69 pKa = 3.72NEEE72 pKa = 4.11AGSSEEE78 pKa = 3.87GEEE81 pKa = 4.72ISNTPGTCEEE91 pKa = 3.59EE92 pKa = 4.42KKK94 pKa = 10.83GEEE97 pKa = 4.17KK98 pKa = 9.68DDD100 pKa = 4.14YY101 pKa = 10.87YY102 pKa = 11.55DD103 pKa = 3.58TCDDD107 pKa = 3.3GVTVTTPDDD116 pKa = 3.08VEEE119 pKa = 4.33EE120 pKa = 4.11SCPDDD125 pKa = 3.43YYY127 pKa = 9.62LVGRR131 pKa = 11.84TCTKKK136 pKa = 10.37LTQPADDD143 pKa = 3.47TYYY146 pKa = 8.44CPDDD150 pKa = 3.27GVLQ

Molecular weight: 16.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L8FTZ4|L8FTZ4_PSED2 ANK_REP_REGION domain-containing protein OS=Pseudogymnoascus destructans (strain ATCC MYA-4855 / 20631-21) OX=658429 GN=GMDG_06002 PE=4 SV=1
RR1 pKa = 6.72GTRR4 pKa = 11.84GLGPIAADD12 pKa = 4.19LPAPLPLSRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84PGGRR27 pKa = 11.84RR28 pKa = 11.84APARR32 pKa = 11.84DD33 pKa = 3.5RR34 pKa = 11.84DD35 pKa = 3.8PGRR38 pKa = 11.84RR39 pKa = 11.84AATGRR44 pKa = 11.84AGAAARR50 pKa = 11.84GAGGAAQPRR59 pKa = 11.84LVGPGGRR66 pKa = 11.84RR67 pKa = 11.84PAAEE71 pKa = 3.68RR72 pKa = 11.84GHH74 pKa = 5.74RR75 pKa = 11.84QRR77 pKa = 11.84SLHH80 pKa = 6.37RR81 pKa = 11.84PALPRR86 pKa = 11.84RR87 pKa = 11.84PGGSRR92 pKa = 11.84PVRR95 pKa = 11.84GRR97 pKa = 11.84GRR99 pKa = 11.84RR100 pKa = 11.84VRR102 pKa = 11.84AARR105 pKa = 11.84ALRR108 pKa = 11.84RR109 pKa = 11.84SRR111 pKa = 11.84RR112 pKa = 11.84IRR114 pKa = 11.84GGPRR118 pKa = 2.71

Molecular weight: 12.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9073

49

9122

4068599

49

4962

446.0

49.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.967 ± 0.028

1.117 ± 0.011

5.62 ± 0.018

6.547 ± 0.028

3.48 ± 0.016

7.141 ± 0.028

2.265 ± 0.011

4.784 ± 0.018

5.175 ± 0.024

8.643 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.214 ± 0.01

3.609 ± 0.013

6.168 ± 0.029

3.947 ± 0.022

6.257 ± 0.024

8.118 ± 0.027

5.986 ± 0.016

6.042 ± 0.02

1.312 ± 0.01

2.606 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski