Oceanospirillum linum
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3358 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T1HDY8|A0A1T1HDY8_OCELI Intracellular proteinase I OS=Oceanospirillum linum OX=966 GN=BTA35_0200210 PE=4 SV=1
MM1 pKa = 7.44 NPFPIFIDD9 pKa = 4.37 FEE11 pKa = 4.54 CSSPDD16 pKa = 3.27 EE17 pKa = 4.94 DD18 pKa = 3.92 GFPIAVCWSTPDD30 pKa = 3.62 GQVKK34 pKa = 8.56 STLITPEE41 pKa = 4.59 DD42 pKa = 3.7 DD43 pKa = 3.29 WLDD46 pKa = 3.69 SHH48 pKa = 6.91 SHH50 pKa = 5.21 QFDD53 pKa = 3.64 DD54 pKa = 4.67 AAIDD58 pKa = 3.59 IDD60 pKa = 4.8 DD61 pKa = 4.54 LMLHH65 pKa = 6.25 GVSALEE71 pKa = 4.73 VIRR74 pKa = 11.84 EE75 pKa = 4.08 LQGDD79 pKa = 3.67 IDD81 pKa = 4.65 SDD83 pKa = 3.46 IVYY86 pKa = 10.43 VDD88 pKa = 3.58 GSGEE92 pKa = 4.05 DD93 pKa = 3.8 EE94 pKa = 4.55 HH95 pKa = 7.93 WLDD98 pKa = 4.08 MLFDD102 pKa = 4.57 SYY104 pKa = 11.9 QMNVQFAFEE113 pKa = 4.24 PAASLYY119 pKa = 10.68 EE120 pKa = 3.75 IGFEE124 pKa = 3.68 HH125 pKa = 6.27 WQDD128 pKa = 4.49 LRR130 pKa = 11.84 SEE132 pKa = 4.04 WMAEE136 pKa = 3.67 RR137 pKa = 11.84 GMDD140 pKa = 4.06 PYY142 pKa = 11.06 QSEE145 pKa = 4.64 ANVLSMLHH153 pKa = 5.01 VHH155 pKa = 6.26 QALTEE160 pKa = 4.3 GGVV163 pKa = 3.06
Molecular weight: 18.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.719
IPC_protein 3.719
Toseland 3.503
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.554
Grimsley 3.414
Solomon 3.706
Lehninger 3.656
Nozaki 3.834
DTASelect 4.062
Thurlkill 3.567
EMBOSS 3.668
Sillero 3.846
Patrickios 1.062
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.753
Protein with the highest isoelectric point:
>tr|A0A1T1HCD3|A0A1T1HCD3_OCELI Acyl-CoA dehydrogenase OS=Oceanospirillum linum OX=966 GN=BTA35_0205625 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.14 NGRR28 pKa = 11.84 KK29 pKa = 9.28 IITRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.54 GRR39 pKa = 11.84 VRR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3358
0
3358
1056198
29
3587
314.5
34.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.127 ± 0.051
0.972 ± 0.014
5.667 ± 0.048
6.543 ± 0.043
3.846 ± 0.028
7.113 ± 0.037
2.265 ± 0.022
5.838 ± 0.03
4.671 ± 0.039
10.918 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.637 ± 0.023
3.6 ± 0.026
4.228 ± 0.03
4.896 ± 0.036
5.309 ± 0.036
6.482 ± 0.038
5.105 ± 0.035
6.799 ± 0.036
1.229 ± 0.017
2.757 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here