Cronobacter phage phiES15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6PBN6|I6PBN6_9CAUD Putative major tail protein OS=Cronobacter phage phiES15 OX=1168280 GN=phiES15_039 PE=4 SV=1
MM1 pKa = 7.58SGPDD5 pKa = 3.5GVKK8 pKa = 10.12VGEE11 pKa = 4.58TITLTAAVEE20 pKa = 4.02PADD23 pKa = 3.84AEE25 pKa = 4.76SYY27 pKa = 8.8TLAWSVDD34 pKa = 3.68DD35 pKa = 4.47EE36 pKa = 4.58AVATIDD42 pKa = 3.43PKK44 pKa = 9.6TGVMEE49 pKa = 4.44GVAAGTAAAVCTAQNSDD66 pKa = 3.09GSKK69 pKa = 9.95VASEE73 pKa = 3.8AHH75 pKa = 5.93AVTVSAPEE83 pKa = 3.58

Molecular weight: 8.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6PDJ6|I6PDJ6_9CAUD Terminase small subunit OS=Cronobacter phage phiES15 OX=1168280 GN=phiES15_028 PE=4 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84GFFNGPHH9 pKa = 6.43PAAFAMNEE17 pKa = 4.35KK18 pKa = 8.84ITIHH22 pKa = 5.8YY23 pKa = 9.14APHH26 pKa = 6.39GLGAIQLALASFYY39 pKa = 10.83EE40 pKa = 4.22QLHH43 pKa = 6.05SLTLYY48 pKa = 9.8EE49 pKa = 4.13WALVTTLIISPLAFIASIVFQWRR72 pKa = 11.84QTRR75 pKa = 11.84AIEE78 pKa = 3.74RR79 pKa = 11.84AARR82 pKa = 11.84EE83 pKa = 4.07GRR85 pKa = 11.84VVAKK89 pKa = 10.35PRR91 pKa = 11.84MFKK94 pKa = 10.8

Molecular weight: 10.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

11760

66

1102

226.2

25.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.731 ± 0.432

1.122 ± 0.141

5.876 ± 0.302

6.182 ± 0.346

3.299 ± 0.169

7.228 ± 0.325

1.667 ± 0.203

5.519 ± 0.21

5.561 ± 0.368

8.605 ± 0.371

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.423 ± 0.116

4.362 ± 0.251

3.801 ± 0.277

4.337 ± 0.349

5.927 ± 0.297

6.063 ± 0.342

6.386 ± 0.313

6.063 ± 0.319

1.743 ± 0.161

3.104 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski