Amino acid dipepetide frequency for Cronobacter phage phiES15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.011AlaAla: 13.011 ± 1.322
0.765AlaCys: 0.765 ± 0.292
6.718AlaAsp: 6.718 ± 0.848
7.143AlaGlu: 7.143 ± 1.001
3.487AlaPhe: 3.487 ± 0.442
7.569AlaGly: 7.569 ± 0.818
1.786AlaHis: 1.786 ± 0.377
5.443AlaIle: 5.443 ± 0.838
6.123AlaLys: 6.123 ± 0.684
8.929AlaLeu: 8.929 ± 0.939
2.466AlaMet: 2.466 ± 0.502
4.932AlaAsn: 4.932 ± 0.82
2.466AlaPro: 2.466 ± 0.533
5.358AlaGln: 5.358 ± 0.812
5.783AlaArg: 5.783 ± 0.691
6.973AlaSer: 6.973 ± 0.868
6.378AlaThr: 6.378 ± 1.03
7.569AlaVal: 7.569 ± 1.108
2.381AlaTrp: 2.381 ± 0.357
2.041AlaTyr: 2.041 ± 0.47
0.0AlaXaa: 0.0 ± 0.0
Cys
1.276CysAla: 1.276 ± 0.429
0.255CysCys: 0.255 ± 0.157
0.595CysAsp: 0.595 ± 0.221
0.425CysGlu: 0.425 ± 0.19
0.425CysPhe: 0.425 ± 0.165
1.106CysGly: 1.106 ± 0.327
0.17CysHis: 0.17 ± 0.117
0.595CysIle: 0.595 ± 0.208
0.34CysLys: 0.34 ± 0.173
0.68CysLeu: 0.68 ± 0.243
0.085CysMet: 0.085 ± 0.107
0.34CysAsn: 0.34 ± 0.181
0.255CysPro: 0.255 ± 0.148
0.51CysGln: 0.51 ± 0.227
1.276CysArg: 1.276 ± 0.357
0.595CysSer: 0.595 ± 0.249
0.595CysThr: 0.595 ± 0.22
0.765CysVal: 0.765 ± 0.291
0.595CysTrp: 0.595 ± 0.271
0.34CysTyr: 0.34 ± 0.181
0.0CysXaa: 0.0 ± 0.0
Asp
5.443AspAla: 5.443 ± 0.753
0.85AspCys: 0.85 ± 0.304
2.976AspAsp: 2.976 ± 0.487
4.592AspGlu: 4.592 ± 0.705
2.126AspPhe: 2.126 ± 0.495
6.973AspGly: 6.973 ± 1.179
0.85AspHis: 0.85 ± 0.32
2.891AspIle: 2.891 ± 0.418
2.806AspLys: 2.806 ± 0.694
4.677AspLeu: 4.677 ± 0.584
1.276AspMet: 1.276 ± 0.226
2.976AspAsn: 2.976 ± 0.646
2.126AspPro: 2.126 ± 0.424
2.126AspGln: 2.126 ± 0.446
2.976AspArg: 2.976 ± 0.586
2.551AspSer: 2.551 ± 0.437
2.721AspThr: 2.721 ± 0.7
3.997AspVal: 3.997 ± 0.727
1.106AspTrp: 1.106 ± 0.36
2.721AspTyr: 2.721 ± 0.506
0.0AspXaa: 0.0 ± 0.0
Glu
7.399GluAla: 7.399 ± 0.905
0.595GluCys: 0.595 ± 0.289
2.551GluAsp: 2.551 ± 0.391
1.701GluGlu: 1.701 ± 0.383
2.891GluPhe: 2.891 ± 0.443
3.572GluGly: 3.572 ± 0.799
1.02GluHis: 1.02 ± 0.303
3.232GluIle: 3.232 ± 0.471
3.402GluLys: 3.402 ± 0.512
7.824GluLeu: 7.824 ± 0.961
2.211GluMet: 2.211 ± 0.509
2.806GluAsn: 2.806 ± 0.346
2.466GluPro: 2.466 ± 0.482
2.976GluGln: 2.976 ± 0.765
3.742GluArg: 3.742 ± 0.508
3.061GluSer: 3.061 ± 0.518
4.167GluThr: 4.167 ± 0.642
3.487GluVal: 3.487 ± 0.596
0.935GluTrp: 0.935 ± 0.32
1.786GluTyr: 1.786 ± 0.326
0.0GluXaa: 0.0 ± 0.0
Phe
3.317PheAla: 3.317 ± 0.552
0.51PheCys: 0.51 ± 0.192
2.041PheAsp: 2.041 ± 0.404
1.701PheGlu: 1.701 ± 0.471
1.361PhePhe: 1.361 ± 0.426
2.041PheGly: 2.041 ± 0.438
0.51PheHis: 0.51 ± 0.194
1.786PheIle: 1.786 ± 0.385
1.446PheLys: 1.446 ± 0.414
2.126PheLeu: 2.126 ± 0.445
0.68PheMet: 0.68 ± 0.235
1.616PheAsn: 1.616 ± 0.338
1.531PhePro: 1.531 ± 0.396
1.276PheGln: 1.276 ± 0.309
2.551PheArg: 2.551 ± 0.474
2.721PheSer: 2.721 ± 0.358
2.721PheThr: 2.721 ± 0.473
1.701PheVal: 1.701 ± 0.405
0.595PheTrp: 0.595 ± 0.252
0.765PheTyr: 0.765 ± 0.243
0.0PheXaa: 0.0 ± 0.0
Gly
7.058GlyAla: 7.058 ± 1.037
1.02GlyCys: 1.02 ± 0.261
4.762GlyAsp: 4.762 ± 0.709
4.847GlyGlu: 4.847 ± 0.612
2.466GlyPhe: 2.466 ± 0.484
5.017GlyGly: 5.017 ± 0.818
0.935GlyHis: 0.935 ± 0.323
4.677GlyIle: 4.677 ± 0.775
5.273GlyLys: 5.273 ± 0.731
5.698GlyLeu: 5.698 ± 0.848
2.211GlyMet: 2.211 ± 0.36
3.317GlyAsn: 3.317 ± 0.536
1.191GlyPro: 1.191 ± 0.334
2.551GlyGln: 2.551 ± 0.401
3.827GlyArg: 3.827 ± 0.74
4.082GlySer: 4.082 ± 0.582
4.337GlyThr: 4.337 ± 0.731
5.358GlyVal: 5.358 ± 0.829
0.935GlyTrp: 0.935 ± 0.278
2.721GlyTyr: 2.721 ± 0.457
0.0GlyXaa: 0.0 ± 0.0
His
0.935HisAla: 0.935 ± 0.274
0.085HisCys: 0.085 ± 0.086
0.935HisAsp: 0.935 ± 0.308
0.68HisGlu: 0.68 ± 0.224
0.595HisPhe: 0.595 ± 0.288
0.935HisGly: 0.935 ± 0.245
0.765HisHis: 0.765 ± 0.321
1.786HisIle: 1.786 ± 0.468
0.68HisLys: 0.68 ± 0.35
1.531HisLeu: 1.531 ± 0.458
0.085HisMet: 0.085 ± 0.073
0.51HisAsn: 0.51 ± 0.194
0.595HisPro: 0.595 ± 0.275
0.425HisGln: 0.425 ± 0.186
0.765HisArg: 0.765 ± 0.202
0.935HisSer: 0.935 ± 0.329
1.446HisThr: 1.446 ± 0.285
1.616HisVal: 1.616 ± 0.404
0.425HisTrp: 0.425 ± 0.229
0.935HisTyr: 0.935 ± 0.288
0.0HisXaa: 0.0 ± 0.0
Ile
4.932IleAla: 4.932 ± 0.59
0.68IleCys: 0.68 ± 0.249
4.252IleAsp: 4.252 ± 0.528
3.402IleGlu: 3.402 ± 0.522
1.276IlePhe: 1.276 ± 0.385
4.507IleGly: 4.507 ± 0.742
0.595IleHis: 0.595 ± 0.216
2.636IleIle: 2.636 ± 0.453
3.572IleLys: 3.572 ± 0.61
3.572IleLeu: 3.572 ± 0.639
1.446IleMet: 1.446 ± 0.377
2.976IleAsn: 2.976 ± 0.524
2.296IlePro: 2.296 ± 0.544
2.466IleGln: 2.466 ± 0.499
2.891IleArg: 2.891 ± 0.551
3.912IleSer: 3.912 ± 0.634
4.082IleThr: 4.082 ± 0.601
2.551IleVal: 2.551 ± 0.457
1.106IleTrp: 1.106 ± 0.295
2.041IleTyr: 2.041 ± 0.48
0.0IleXaa: 0.0 ± 0.0
Lys
7.058LysAla: 7.058 ± 0.972
0.85LysCys: 0.85 ± 0.375
2.721LysAsp: 2.721 ± 0.458
3.317LysGlu: 3.317 ± 0.605
1.191LysPhe: 1.191 ± 0.333
3.487LysGly: 3.487 ± 0.563
1.361LysHis: 1.361 ± 0.372
2.636LysIle: 2.636 ± 0.382
2.976LysLys: 2.976 ± 0.665
3.572LysLeu: 3.572 ± 0.648
2.041LysMet: 2.041 ± 0.381
2.041LysAsn: 2.041 ± 0.449
2.806LysPro: 2.806 ± 0.398
2.976LysGln: 2.976 ± 0.515
3.317LysArg: 3.317 ± 0.765
3.742LysSer: 3.742 ± 0.54
3.827LysThr: 3.827 ± 0.772
2.891LysVal: 2.891 ± 0.497
0.85LysTrp: 0.85 ± 0.353
1.956LysTyr: 1.956 ± 0.36
0.0LysXaa: 0.0 ± 0.0
Leu
9.865LeuAla: 9.865 ± 0.998
1.191LeuCys: 1.191 ± 0.427
5.358LeuAsp: 5.358 ± 0.593
5.613LeuGlu: 5.613 ± 0.634
2.976LeuPhe: 2.976 ± 0.659
4.677LeuGly: 4.677 ± 0.538
1.616LeuHis: 1.616 ± 0.376
5.017LeuIle: 5.017 ± 0.843
6.123LeuLys: 6.123 ± 0.866
6.888LeuLeu: 6.888 ± 0.951
1.446LeuMet: 1.446 ± 0.472
4.677LeuAsn: 4.677 ± 0.615
4.167LeuPro: 4.167 ± 0.692
2.296LeuGln: 2.296 ± 0.447
5.358LeuArg: 5.358 ± 0.592
5.528LeuSer: 5.528 ± 0.794
5.358LeuThr: 5.358 ± 0.693
5.188LeuVal: 5.188 ± 0.777
0.425LeuTrp: 0.425 ± 0.202
2.296LeuTyr: 2.296 ± 0.398
0.0LeuXaa: 0.0 ± 0.0
Met
1.956MetAla: 1.956 ± 0.291
0.255MetCys: 0.255 ± 0.149
1.701MetAsp: 1.701 ± 0.423
1.02MetGlu: 1.02 ± 0.254
0.68MetPhe: 0.68 ± 0.213
1.701MetGly: 1.701 ± 0.379
0.51MetHis: 0.51 ± 0.181
1.191MetIle: 1.191 ± 0.28
1.871MetLys: 1.871 ± 0.386
2.211MetLeu: 2.211 ± 0.358
0.34MetMet: 0.34 ± 0.16
1.361MetAsn: 1.361 ± 0.306
1.191MetPro: 1.191 ± 0.285
1.106MetGln: 1.106 ± 0.295
1.446MetArg: 1.446 ± 0.416
2.041MetSer: 2.041 ± 0.496
1.616MetThr: 1.616 ± 0.311
1.106MetVal: 1.106 ± 0.218
0.425MetTrp: 0.425 ± 0.219
0.51MetTyr: 0.51 ± 0.209
0.0MetXaa: 0.0 ± 0.0
Asn
4.422AsnAla: 4.422 ± 0.566
0.085AsnCys: 0.085 ± 0.088
2.466AsnAsp: 2.466 ± 0.523
2.211AsnGlu: 2.211 ± 0.38
1.276AsnPhe: 1.276 ± 0.284
3.827AsnGly: 3.827 ± 0.696
0.68AsnHis: 0.68 ± 0.231
2.211AsnIle: 2.211 ± 0.373
2.551AsnLys: 2.551 ± 0.533
2.806AsnLeu: 2.806 ± 0.625
1.106AsnMet: 1.106 ± 0.293
1.701AsnAsn: 1.701 ± 0.46
2.721AsnPro: 2.721 ± 0.48
2.041AsnGln: 2.041 ± 0.459
2.976AsnArg: 2.976 ± 0.542
3.061AsnSer: 3.061 ± 0.546
3.232AsnThr: 3.232 ± 0.489
2.296AsnVal: 2.296 ± 0.405
0.85AsnTrp: 0.85 ± 0.282
1.106AsnTyr: 1.106 ± 0.324
0.0AsnXaa: 0.0 ± 0.0
Pro
3.657ProAla: 3.657 ± 0.618
0.17ProCys: 0.17 ± 0.133
3.912ProAsp: 3.912 ± 0.565
2.976ProGlu: 2.976 ± 0.507
0.935ProPhe: 0.935 ± 0.255
3.147ProGly: 3.147 ± 0.565
0.935ProHis: 0.935 ± 0.306
1.106ProIle: 1.106 ± 0.311
1.616ProLys: 1.616 ± 0.414
3.232ProLeu: 3.232 ± 0.528
0.595ProMet: 0.595 ± 0.193
1.191ProAsn: 1.191 ± 0.31
1.02ProPro: 1.02 ± 0.536
1.446ProGln: 1.446 ± 0.417
2.551ProArg: 2.551 ± 0.456
2.211ProSer: 2.211 ± 0.516
1.616ProThr: 1.616 ± 0.511
3.572ProVal: 3.572 ± 0.542
1.02ProTrp: 1.02 ± 0.343
1.106ProTyr: 1.106 ± 0.319
0.0ProXaa: 0.0 ± 0.0
Gln
3.997GlnAla: 3.997 ± 0.938
0.51GlnCys: 0.51 ± 0.271
1.276GlnAsp: 1.276 ± 0.3
2.551GlnGlu: 2.551 ± 0.506
1.446GlnPhe: 1.446 ± 0.307
1.871GlnGly: 1.871 ± 0.358
0.85GlnHis: 0.85 ± 0.22
2.551GlnIle: 2.551 ± 0.455
2.636GlnLys: 2.636 ± 0.806
4.677GlnLeu: 4.677 ± 0.616
1.531GlnMet: 1.531 ± 0.414
1.701GlnAsn: 1.701 ± 0.348
1.446GlnPro: 1.446 ± 0.479
3.402GlnGln: 3.402 ± 1.136
2.381GlnArg: 2.381 ± 0.513
2.551GlnSer: 2.551 ± 0.418
2.721GlnThr: 2.721 ± 0.46
2.721GlnVal: 2.721 ± 0.418
0.68GlnTrp: 0.68 ± 0.232
1.871GlnTyr: 1.871 ± 0.42
0.0GlnXaa: 0.0 ± 0.0
Arg
7.314ArgAla: 7.314 ± 1.013
0.51ArgCys: 0.51 ± 0.219
2.891ArgAsp: 2.891 ± 0.425
4.167ArgGlu: 4.167 ± 0.791
1.956ArgPhe: 1.956 ± 0.483
3.997ArgGly: 3.997 ± 0.501
0.85ArgHis: 0.85 ± 0.341
4.762ArgIle: 4.762 ± 0.582
3.402ArgLys: 3.402 ± 0.576
6.378ArgLeu: 6.378 ± 0.865
1.361ArgMet: 1.361 ± 0.34
2.126ArgAsn: 2.126 ± 0.481
1.956ArgPro: 1.956 ± 0.38
2.721ArgGln: 2.721 ± 0.429
3.742ArgArg: 3.742 ± 0.692
2.296ArgSer: 2.296 ± 0.427
2.721ArgThr: 2.721 ± 0.484
2.891ArgVal: 2.891 ± 0.403
0.51ArgTrp: 0.51 ± 0.194
2.721ArgTyr: 2.721 ± 0.486
0.0ArgXaa: 0.0 ± 0.0
Ser
6.463SerAla: 6.463 ± 1.105
0.425SerCys: 0.425 ± 0.156
4.507SerAsp: 4.507 ± 0.781
3.997SerGlu: 3.997 ± 0.729
2.126SerPhe: 2.126 ± 0.453
6.208SerGly: 6.208 ± 0.87
1.276SerHis: 1.276 ± 0.319
2.721SerIle: 2.721 ± 0.505
2.466SerLys: 2.466 ± 0.573
5.698SerLeu: 5.698 ± 0.847
1.276SerMet: 1.276 ± 0.261
1.871SerAsn: 1.871 ± 0.366
2.466SerPro: 2.466 ± 0.461
3.061SerGln: 3.061 ± 0.324
2.806SerArg: 2.806 ± 0.467
3.061SerSer: 3.061 ± 0.554
3.061SerThr: 3.061 ± 0.573
3.827SerVal: 3.827 ± 0.572
1.616SerTrp: 1.616 ± 0.316
1.701SerTyr: 1.701 ± 0.434
0.0SerXaa: 0.0 ± 0.0
Thr
8.164ThrAla: 8.164 ± 1.056
0.85ThrCys: 0.85 ± 0.31
3.147ThrAsp: 3.147 ± 0.493
4.167ThrGlu: 4.167 ± 0.549
2.296ThrPhe: 2.296 ± 0.564
5.613ThrGly: 5.613 ± 0.749
0.765ThrHis: 0.765 ± 0.305
4.507ThrIle: 4.507 ± 0.641
2.126ThrLys: 2.126 ± 0.619
6.293ThrLeu: 6.293 ± 0.69
1.361ThrMet: 1.361 ± 0.384
1.871ThrAsn: 1.871 ± 0.341
2.721ThrPro: 2.721 ± 0.506
1.616ThrGln: 1.616 ± 0.279
3.912ThrArg: 3.912 ± 0.597
3.742ThrSer: 3.742 ± 0.576
4.252ThrThr: 4.252 ± 0.655
3.402ThrVal: 3.402 ± 0.791
1.191ThrTrp: 1.191 ± 0.46
1.871ThrTyr: 1.871 ± 0.269
0.0ThrXaa: 0.0 ± 0.0
Val
6.123ValAla: 6.123 ± 0.801
0.595ValCys: 0.595 ± 0.228
3.827ValAsp: 3.827 ± 0.625
3.742ValGlu: 3.742 ± 0.504
1.871ValPhe: 1.871 ± 0.304
3.061ValGly: 3.061 ± 0.493
0.425ValHis: 0.425 ± 0.239
3.232ValIle: 3.232 ± 0.555
3.827ValLys: 3.827 ± 0.475
4.932ValLeu: 4.932 ± 0.518
1.446ValMet: 1.446 ± 0.339
4.252ValAsn: 4.252 ± 0.769
1.956ValPro: 1.956 ± 0.43
2.296ValGln: 2.296 ± 0.446
3.742ValArg: 3.742 ± 0.53
4.337ValSer: 4.337 ± 0.564
5.613ValThr: 5.613 ± 0.86
3.232ValVal: 3.232 ± 0.637
0.765ValTrp: 0.765 ± 0.302
1.361ValTyr: 1.361 ± 0.224
0.0ValXaa: 0.0 ± 0.0
Trp
1.446TrpAla: 1.446 ± 0.285
0.425TrpCys: 0.425 ± 0.172
0.51TrpAsp: 0.51 ± 0.267
1.361TrpGlu: 1.361 ± 0.394
0.51TrpPhe: 0.51 ± 0.186
0.935TrpGly: 0.935 ± 0.459
0.255TrpHis: 0.255 ± 0.151
0.765TrpIle: 0.765 ± 0.22
1.02TrpLys: 1.02 ± 0.279
1.956TrpLeu: 1.956 ± 0.556
0.34TrpMet: 0.34 ± 0.195
0.595TrpAsn: 0.595 ± 0.274
1.106TrpPro: 1.106 ± 0.353
0.85TrpGln: 0.85 ± 0.229
1.616TrpArg: 1.616 ± 0.36
1.191TrpSer: 1.191 ± 0.408
0.935TrpThr: 0.935 ± 0.352
0.765TrpVal: 0.765 ± 0.273
0.34TrpTrp: 0.34 ± 0.165
0.425TrpTyr: 0.425 ± 0.182
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.487TyrAla: 3.487 ± 0.57
0.595TyrCys: 0.595 ± 0.204
1.616TyrAsp: 1.616 ± 0.241
2.211TyrGlu: 2.211 ± 0.404
1.106TyrPhe: 1.106 ± 0.418
2.041TyrGly: 2.041 ± 0.406
0.51TyrHis: 0.51 ± 0.227
1.446TyrIle: 1.446 ± 0.373
1.361TyrLys: 1.361 ± 0.317
2.381TyrLeu: 2.381 ± 0.432
0.85TyrMet: 0.85 ± 0.259
0.765TyrAsn: 0.765 ± 0.222
1.531TyrPro: 1.531 ± 0.4
1.871TyrGln: 1.871 ± 0.417
1.616TyrArg: 1.616 ± 0.371
2.041TyrSer: 2.041 ± 0.389
2.466TyrThr: 2.466 ± 0.478
1.701TyrVal: 1.701 ± 0.402
0.68TyrTrp: 0.68 ± 0.241
0.765TyrTyr: 0.765 ± 0.3
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (11760 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski