Maritimibacter sp. 55A14
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3708 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U2CAH2|A0A2U2CAH2_9RHOB Glycerol-3-phosphate dehydrogenase [NAD(P)+] OS=Maritimibacter sp. 55A14 OX=2174844 GN=gpsA PE=3 SV=1
MM1 pKa = 7.33 TLDD4 pKa = 3.75 ASQPLEE10 pKa = 4.06 GTPLIKK16 pKa = 10.2 PSSTDD21 pKa = 2.87 HH22 pKa = 6.94 PLYY25 pKa = 10.15 DD26 pKa = 4.43 TIVEE30 pKa = 4.27 ACRR33 pKa = 11.84 AVYY36 pKa = 10.34 DD37 pKa = 3.75 PEE39 pKa = 5.16 IPVNIYY45 pKa = 10.8 DD46 pKa = 4.42 LGLIYY51 pKa = 10.17 TIEE54 pKa = 4.88 IGDD57 pKa = 3.9 EE58 pKa = 4.12 GDD60 pKa = 3.58 VVIVMTLTAPGCPVAGEE77 pKa = 4.15 MPGWVADD84 pKa = 3.87 AVEE87 pKa = 4.35 PLQGVRR93 pKa = 11.84 TVSVEE98 pKa = 4.25 LVWDD102 pKa = 4.63 PPWGMDD108 pKa = 3.35 MMSDD112 pKa = 3.68 EE113 pKa = 4.89 ARR115 pKa = 11.84 LEE117 pKa = 4.14 LGFMM121 pKa = 4.54
Molecular weight: 13.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.643
IPC_protein 3.592
Toseland 3.401
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.528
Rodwell 3.439
Grimsley 3.312
Solomon 3.567
Lehninger 3.528
Nozaki 3.719
DTASelect 3.91
Thurlkill 3.465
EMBOSS 3.541
Sillero 3.719
Patrickios 0.769
IPC_peptide 3.567
IPC2_peptide 3.694
IPC2.peptide.svr19 3.684
Protein with the highest isoelectric point:
>tr|A0A2U2CLM5|A0A2U2CLM5_9RHOB Phage portal protein OS=Maritimibacter sp. 55A14 OX=2174844 GN=DDZ14_08475 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 QILNRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 9.25 KK41 pKa = 10.65 LSAA44 pKa = 3.91
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3708
0
3708
1118195
30
2839
301.6
32.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.209 ± 0.066
0.965 ± 0.014
5.953 ± 0.029
6.164 ± 0.037
3.624 ± 0.029
9.01 ± 0.037
2.148 ± 0.019
4.788 ± 0.03
2.695 ± 0.031
10.106 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.692 ± 0.018
2.275 ± 0.019
5.282 ± 0.028
2.751 ± 0.021
7.755 ± 0.04
4.667 ± 0.028
5.15 ± 0.029
7.191 ± 0.033
1.446 ± 0.017
2.13 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here