Perkinsus sp. BL_2016

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Perkinsozoa; Perkinsea; Perkinsida; Perkinsidae; Perkinsus; unclassified Perkinsus

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 691 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D8XLH6|A0A4D8XLH6_9ALVE Uncharacterized protein OS=Perkinsus sp. BL_2016 OX=2494336 GN=C9890_0299 PE=4 SV=1
MM1 pKa = 6.4TTCNSYY7 pKa = 11.11CSDD10 pKa = 3.5FVSGSNCQLWLPIPLCQGTDD30 pKa = 3.3VRR32 pKa = 11.84CGYY35 pKa = 10.33DD36 pKa = 2.93SCRR39 pKa = 11.84VNSRR43 pKa = 11.84PSRR46 pKa = 11.84QIVAKK51 pKa = 10.05SATSSHH57 pKa = 6.11SVTLTRR63 pKa = 11.84IIVSGSYY70 pKa = 7.89PTSTPTSPTMMTTIIPEE87 pKa = 4.5GSATNSHH94 pKa = 6.28SADD97 pKa = 3.16LTQIVVDD104 pKa = 5.13GPHH107 pKa = 7.04PGSTTTTTASSEE119 pKa = 4.41TTPEE123 pKa = 3.71APMSVSVVPITSQDD137 pKa = 3.33PTATASTTEE146 pKa = 4.13SEE148 pKa = 4.54APMSVSAVPISSSDD162 pKa = 3.06PSMAYY167 pKa = 10.34VSVAEE172 pKa = 4.21STEE175 pKa = 4.61DD176 pKa = 3.8IADD179 pKa = 3.76SDD181 pKa = 4.1PLEE184 pKa = 4.39NCISRR189 pKa = 11.84AEE191 pKa = 3.94SDD193 pKa = 3.45EE194 pKa = 5.54NIFLWAEE201 pKa = 3.96WPSIPSEE208 pKa = 3.95ANSWINYY215 pKa = 6.72YY216 pKa = 11.08SSMASFIDD224 pKa = 4.32GNCARR229 pKa = 11.84LKK231 pKa = 8.44VTRR234 pKa = 11.84LILRR238 pKa = 11.84VTDD241 pKa = 4.08PLKK244 pKa = 10.64GAWWPPLDD252 pKa = 3.73SPMYY256 pKa = 10.01TSLLRR261 pKa = 11.84NIPEE265 pKa = 3.86EE266 pKa = 4.36TEE268 pKa = 4.36VYY270 pKa = 9.97VYY272 pKa = 10.07PYY274 pKa = 11.67VMDD277 pKa = 3.95QWNQDD282 pKa = 2.57QWTCFSPSNTSVLEE296 pKa = 4.08GAFEE300 pKa = 4.4FAKK303 pKa = 10.22RR304 pKa = 11.84WNDD307 pKa = 3.13FLTQQTSSLRR317 pKa = 11.84FVGIVLDD324 pKa = 3.83AEE326 pKa = 4.43EE327 pKa = 4.56FSGGRR332 pKa = 11.84NPEE335 pKa = 3.69FRR337 pKa = 11.84AQLLNSTQLKK347 pKa = 9.49QKK349 pKa = 10.7YY350 pKa = 7.71SLKK353 pKa = 10.69LGLSLGYY360 pKa = 10.37QSGPQMAAWDD370 pKa = 4.04SVVDD374 pKa = 3.73AFYY377 pKa = 10.98LQFYY381 pKa = 9.85DD382 pKa = 4.15YY383 pKa = 10.85YY384 pKa = 8.26YY385 pKa = 10.46TPYY388 pKa = 10.8VDD390 pKa = 4.26ATADD394 pKa = 3.91SPFLLYY400 pKa = 11.14LNDD403 pKa = 3.99PASLVGFTLDD413 pKa = 3.23TVLEE417 pKa = 4.71GKK419 pKa = 9.97SEE421 pKa = 4.12DD422 pKa = 3.46QTKK425 pKa = 10.39YY426 pKa = 9.65GPKK429 pKa = 9.97VSVMWSMQNLGTGCIFPLNDD449 pKa = 3.7GSCGVNFEE457 pKa = 5.28FGAGWSAQAFNEE469 pKa = 3.94YY470 pKa = 10.42LKK472 pKa = 9.9EE473 pKa = 3.87FRR475 pKa = 11.84MRR477 pKa = 11.84SPNLGYY483 pKa = 10.24KK484 pKa = 9.07PQGIFQYY491 pKa = 11.16SFLPQSWFLTT501 pKa = 4.06

Molecular weight: 55.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D8XPI3|A0A4D8XPI3_9ALVE Uncharacterized protein OS=Perkinsus sp. BL_2016 OX=2494336 GN=C9890_0706 PE=4 SV=1
SS1 pKa = 6.82SGRR4 pKa = 11.84SLRR7 pKa = 11.84SRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ACRR16 pKa = 11.84RR17 pKa = 11.84ARR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 9.83PDD23 pKa = 2.82VHH25 pKa = 7.53LPLGRR30 pKa = 11.84TGMARR35 pKa = 11.84RR36 pKa = 11.84PPAARR41 pKa = 11.84PRR43 pKa = 11.84WHH45 pKa = 7.15RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84SLQRR52 pKa = 11.84TPLRR56 pKa = 11.84GHH58 pKa = 6.59RR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84SPPRR65 pKa = 11.84HH66 pKa = 5.49LAAYY70 pKa = 7.2DD71 pKa = 3.76HH72 pKa = 7.08PPAPVFALHH81 pKa = 5.32SHH83 pKa = 5.34RR84 pKa = 11.84HH85 pKa = 4.73RR86 pKa = 11.84LRR88 pKa = 11.84RR89 pKa = 11.84LASGARR95 pKa = 11.84RR96 pKa = 11.84TRR98 pKa = 11.84PHH100 pKa = 6.79RR101 pKa = 11.84RR102 pKa = 11.84PNNLPGFRR110 pKa = 11.84PLRR113 pKa = 11.84PGPRR117 pKa = 11.84TGNVPRR123 pKa = 11.84MRR125 pKa = 11.84RR126 pKa = 11.84RR127 pKa = 11.84HH128 pKa = 5.89APPSQPSRR136 pKa = 11.84PRR138 pKa = 11.84RR139 pKa = 11.84IAPRR143 pKa = 11.84TTQRR147 pKa = 11.84RR148 pKa = 11.84QSPRR152 pKa = 11.84SRR154 pKa = 11.84GPPRR158 pKa = 11.84SRR160 pKa = 11.84PTPRR164 pKa = 11.84HH165 pKa = 5.64PPRR168 pKa = 11.84SRR170 pKa = 11.84SPRR173 pKa = 11.84PLPTT177 pKa = 3.53

Molecular weight: 20.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

691

0

691

233055

44

2322

337.3

37.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.038 ± 0.131

1.719 ± 0.044

5.326 ± 0.07

6.202 ± 0.095

4.339 ± 0.069

6.341 ± 0.094

2.28 ± 0.046

5.766 ± 0.074

5.306 ± 0.099

9.507 ± 0.089

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.294 ± 0.044

3.996 ± 0.069

4.836 ± 0.097

3.613 ± 0.055

5.752 ± 0.098

8.73 ± 0.145

5.408 ± 0.073

6.883 ± 0.076

1.137 ± 0.032

2.524 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski