Arboricoccus pini
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3717 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A212RWQ3|A0A212RWQ3_9PROT DNA-binding transcriptional regulator LysR family OS=Arboricoccus pini OX=1963835 GN=SAMN07250955_11638 PE=3 SV=1
MM1 pKa = 7.31 SADD4 pKa = 3.17 SATRR8 pKa = 11.84 ARR10 pKa = 11.84 DD11 pKa = 3.45 MIDD14 pKa = 3.33 NIGINIHH21 pKa = 6.79 LSYY24 pKa = 11.18 TDD26 pKa = 3.93 GDD28 pKa = 4.01 YY29 pKa = 11.46 KK30 pKa = 11.34 NYY32 pKa = 10.75 DD33 pKa = 3.42 SVIADD38 pKa = 4.43 LGYY41 pKa = 11.09 LGITLVRR48 pKa = 11.84 DD49 pKa = 3.65 RR50 pKa = 11.84 VPNPNTNGGGGKK62 pKa = 10.06 NYY64 pKa = 10.61 GYY66 pKa = 10.53 LADD69 pKa = 4.04 AGIRR73 pKa = 11.84 FDD75 pKa = 5.1 LNASAAASDD84 pKa = 4.13 FAGTMTLIEE93 pKa = 4.6 NFVAAHH99 pKa = 6.66 PGSVVAIEE107 pKa = 4.47 GANEE111 pKa = 3.68 VDD113 pKa = 4.33 KK114 pKa = 11.81 NPVSYY119 pKa = 11.0 DD120 pKa = 3.13 GLTGQAAALALQSDD134 pKa = 4.4 IYY136 pKa = 11.11 DD137 pKa = 3.75 YY138 pKa = 11.83 VHH140 pKa = 7.0 TSTTLEE146 pKa = 5.38 GIATWDD152 pKa = 3.28 LTGITGGTDD161 pKa = 3.26 FADD164 pKa = 3.56 ASNAHH169 pKa = 7.0 IYY171 pKa = 8.77 PRR173 pKa = 11.84 NGNQPQDD180 pKa = 3.46 RR181 pKa = 11.84 LGLAIEE187 pKa = 4.48 TTLAAQPDD195 pKa = 4.11 RR196 pKa = 11.84 PMVITEE202 pKa = 4.31 GGYY205 pKa = 8.45 YY206 pKa = 9.38 TLPGQGWGGVDD217 pKa = 3.12 ATTQSIYY224 pKa = 10.19 TINYY228 pKa = 8.95 LLDD231 pKa = 3.79 AVSQGVSTTFLYY243 pKa = 10.68 QLLDD247 pKa = 3.81 AYY249 pKa = 9.92 TDD251 pKa = 3.52 TTGTDD256 pKa = 3.12 IEE258 pKa = 4.47 KK259 pKa = 10.74 HH260 pKa = 5.8 FGLFDD265 pKa = 4.64 ADD267 pKa = 4.0 NNPKK271 pKa = 9.22 QAAVALHH278 pKa = 6.43 NFTTILSDD286 pKa = 3.53 TGASATTFAVDD297 pKa = 3.17 TLDD300 pKa = 4.0 YY301 pKa = 11.3 DD302 pKa = 3.91 VTGWNSYY309 pKa = 7.3 GHH311 pKa = 6.54 SYY313 pKa = 10.95 LFEE316 pKa = 6.17 KK317 pKa = 10.79 SDD319 pKa = 3.5 GTFDD323 pKa = 2.99 IALWYY328 pKa = 9.36 EE329 pKa = 3.52 QDD331 pKa = 3.04 IWDD334 pKa = 4.37 EE335 pKa = 4.39 VNLVPIATTSTAIDD349 pKa = 3.96 LALGSYY355 pKa = 9.79 FRR357 pKa = 11.84 VVEE360 pKa = 4.15 VFDD363 pKa = 4.21 PQLGTGAIATYY374 pKa = 10.64 HH375 pKa = 6.86 DD376 pKa = 4.55 VDD378 pKa = 4.76 HH379 pKa = 7.4 LDD381 pKa = 3.7 LLVNQDD387 pKa = 3.48 ALIVQLEE394 pKa = 4.28 PNGANAAPTIEE405 pKa = 4.32 GVNPQQVVVGDD416 pKa = 4.35 DD417 pKa = 3.51 LTLSFTSDD425 pKa = 3.58 MISDD429 pKa = 4.05 ANGDD433 pKa = 4.03 TVLLNATLANGSALPSWLTFDD454 pKa = 4.58 PDD456 pKa = 3.0 TWTLSGVATQTGKK469 pKa = 10.13 WSILLTASDD478 pKa = 4.42 HH479 pKa = 6.21 YY480 pKa = 11.32 GGASSLVFQLSVTSQDD496 pKa = 3.54 HH497 pKa = 5.76 EE498 pKa = 4.26 QTPTITGDD506 pKa = 3.22 GTIIGTKK513 pKa = 9.77 AADD516 pKa = 3.82 VIAGGDD522 pKa = 3.42 NSDD525 pKa = 4.53 TINASSGADD534 pKa = 2.72 IVYY537 pKa = 10.05 GHH539 pKa = 7.57 AGNDD543 pKa = 4.05 LINGGGDD550 pKa = 3.47 ADD552 pKa = 4.41 DD553 pKa = 5.34 LYY555 pKa = 11.64 GGAGNDD561 pKa = 3.79 TLRR564 pKa = 11.84 GDD566 pKa = 3.77 AGADD570 pKa = 3.19 RR571 pKa = 11.84 LFGQGGDD578 pKa = 3.59 DD579 pKa = 3.57 TLAGGGGGFDD589 pKa = 5.04 LLTGGAGADD598 pKa = 3.3 TFIFRR603 pKa = 11.84 GKK605 pKa = 10.23 EE606 pKa = 3.5 STGPVEE612 pKa = 4.21 SVEE615 pKa = 4.1 GFLVVQHH622 pKa = 6.41 ARR624 pKa = 11.84 IADD627 pKa = 3.67 LDD629 pKa = 3.84 FAEE632 pKa = 4.92 GDD634 pKa = 3.8 RR635 pKa = 11.84 LQLSYY640 pKa = 10.7 FVDD643 pKa = 3.49 SSGVSLQKK651 pKa = 10.89 AVGLSANIDD660 pKa = 3.83 SLSGLGTLVHH670 pKa = 6.87 FLEE673 pKa = 5.04 TDD675 pKa = 3.57 DD676 pKa = 4.68 PSHH679 pKa = 6.85 VIADD683 pKa = 4.05 ASTDD687 pKa = 3.52 TLTLILKK694 pKa = 9.94 DD695 pKa = 3.9 SNGGLHH701 pKa = 6.92 ALEE704 pKa = 4.61 LLHH707 pKa = 6.46 YY708 pKa = 9.25 ATIAA712 pKa = 3.26
Molecular weight: 74.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.681
IPC2_protein 3.795
IPC_protein 3.859
Toseland 3.617
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.834
Rodwell 3.681
Grimsley 3.516
Solomon 3.872
Lehninger 3.821
Nozaki 3.973
DTASelect 4.291
Thurlkill 3.681
EMBOSS 3.834
Sillero 3.986
Patrickios 1.545
IPC_peptide 3.859
IPC2_peptide 3.961
IPC2.peptide.svr19 3.868
Protein with the highest isoelectric point:
>tr|A0A212QSZ5|A0A212QSZ5_9PROT Uncharacterized protein OS=Arboricoccus pini OX=1963835 GN=SAMN07250955_103173 PE=4 SV=1
MM1 pKa = 7.44 LVLGLTGSLAMGKK14 pKa = 9.57 SRR16 pKa = 11.84 TAAMFMAHH24 pKa = 7.73 GIPVFDD30 pKa = 4.68 SDD32 pKa = 3.9 ATVHH36 pKa = 6.3 GLYY39 pKa = 10.58 APGGAAVLPILRR51 pKa = 11.84 LFEE54 pKa = 5.02 DD55 pKa = 4.53 CGSVSDD61 pKa = 5.08 GIDD64 pKa = 2.99 RR65 pKa = 11.84 AALGRR70 pKa = 11.84 RR71 pKa = 11.84 ILSDD75 pKa = 3.49 DD76 pKa = 3.19 TAMRR80 pKa = 11.84 ALEE83 pKa = 4.22 RR84 pKa = 11.84 VVHH87 pKa = 5.83 ALVRR91 pKa = 11.84 SSQRR95 pKa = 11.84 AFLEE99 pKa = 4.21 TQARR103 pKa = 11.84 LGHH106 pKa = 6.81 WLTVLDD112 pKa = 4.52 IPLLYY117 pKa = 10.54 EE118 pKa = 3.92 SGGDD122 pKa = 3.5 RR123 pKa = 11.84 RR124 pKa = 11.84 CDD126 pKa = 3.48 RR127 pKa = 11.84 IVVVSASPFIQSQRR141 pKa = 11.84 ALARR145 pKa = 11.84 TGMTALRR152 pKa = 11.84 LKK154 pKa = 10.71 SLLEE158 pKa = 4.03 RR159 pKa = 11.84 QTPDD163 pKa = 4.14 LRR165 pKa = 11.84 KK166 pKa = 9.37 QCLADD171 pKa = 4.1 FVIHH175 pKa = 6.38 TGGDD179 pKa = 3.07 RR180 pKa = 11.84 GRR182 pKa = 11.84 LSRR185 pKa = 11.84 NVAEE189 pKa = 5.18 ILRR192 pKa = 11.84 KK193 pKa = 8.48 MRR195 pKa = 11.84 QVQPHH200 pKa = 6.84 AWPKK204 pKa = 9.62 RR205 pKa = 11.84 WW206 pKa = 3.14
Molecular weight: 22.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.399
IPC_protein 10.365
Toseland 10.672
ProMoST 10.496
Dawson 10.73
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.745
Grimsley 10.774
Solomon 10.935
Lehninger 10.891
Nozaki 10.672
DTASelect 10.496
Thurlkill 10.657
EMBOSS 11.082
Sillero 10.687
Patrickios 10.496
IPC_peptide 10.95
IPC2_peptide 9.78
IPC2.peptide.svr19 8.741
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3717
0
3717
1199682
24
2625
322.8
35.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.565 ± 0.054
0.861 ± 0.013
5.639 ± 0.029
5.532 ± 0.036
3.578 ± 0.025
8.572 ± 0.04
2.083 ± 0.018
5.168 ± 0.032
2.912 ± 0.03
11.092 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.435 ± 0.017
2.321 ± 0.021
5.419 ± 0.028
3.263 ± 0.025
7.524 ± 0.043
5.307 ± 0.031
5.101 ± 0.028
7.168 ± 0.03
1.32 ± 0.015
2.14 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here