Tetzosporium hominis
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2916 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A264W087|A0A264W087_9BACL Uncharacterized protein OS=Tetzosporium hominis OX=2020506 GN=CF394_13755 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.3 KK3 pKa = 10.14 VLTGITLGASILALAACGSEE23 pKa = 4.4 GGSSSGDD30 pKa = 3.37 DD31 pKa = 3.26 TTLTVGASNVPHH43 pKa = 7.59 AEE45 pKa = 3.54 ILEE48 pKa = 4.24 QVKK51 pKa = 9.87 PLLEE55 pKa = 4.1 EE56 pKa = 3.66 QGIEE60 pKa = 4.45 LEE62 pKa = 4.01 IEE64 pKa = 4.27 TYY66 pKa = 9.76 QDD68 pKa = 3.47 YY69 pKa = 11.31 VLPNQDD75 pKa = 4.67 LDD77 pKa = 5.08 SGDD80 pKa = 4.43 LDD82 pKa = 4.05 ANYY85 pKa = 9.42 FQHH88 pKa = 6.91 IPYY91 pKa = 10.09 LEE93 pKa = 4.01 SQIADD98 pKa = 3.22 NGYY101 pKa = 10.49 DD102 pKa = 3.51 FVNAGGIHH110 pKa = 6.57 IEE112 pKa = 4.38 PIGLYY117 pKa = 9.49 SKK119 pKa = 10.92 KK120 pKa = 10.33 FGSIDD125 pKa = 3.81 EE126 pKa = 4.96 LPDD129 pKa = 3.39 GATILMSNSVADD141 pKa = 3.79 HH142 pKa = 5.92 GRR144 pKa = 11.84 VLSMLEE150 pKa = 3.57 QAGLIKK156 pKa = 10.35 LDD158 pKa = 3.64 EE159 pKa = 4.57 SVEE162 pKa = 4.23 KK163 pKa = 10.28 TSATIEE169 pKa = 4.08 DD170 pKa = 3.99 VVEE173 pKa = 4.06 NPKK176 pKa = 10.64 NIQFDD181 pKa = 4.18 PNYY184 pKa = 9.77 EE185 pKa = 3.99 AALLVQMYY193 pKa = 8.27 EE194 pKa = 4.06 QEE196 pKa = 4.09 EE197 pKa = 4.38 GDD199 pKa = 3.44 AVLINSNYY207 pKa = 10.76 ALDD210 pKa = 4.69 AGLNPIEE217 pKa = 4.77 DD218 pKa = 4.09 SIAIEE223 pKa = 4.76 DD224 pKa = 3.98 SNSPYY229 pKa = 11.02 VNVIAVNAGDD239 pKa = 4.36 EE240 pKa = 4.18 EE241 pKa = 4.53 NEE243 pKa = 4.23 AIVALVEE250 pKa = 4.16 ALHH253 pKa = 5.91 SQEE256 pKa = 3.76 IQDD259 pKa = 4.21 WILEE263 pKa = 4.2 EE264 pKa = 4.04 WGGSVVPVTEE274 pKa = 4.01
Molecular weight: 29.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.757
IPC_protein 3.732
Toseland 3.541
ProMoST 3.859
Dawson 3.706
Bjellqvist 3.884
Wikipedia 3.592
Rodwell 3.567
Grimsley 3.452
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 3.986
Thurlkill 3.579
EMBOSS 3.617
Sillero 3.846
Patrickios 1.151
IPC_peptide 3.694
IPC2_peptide 3.834
IPC2.peptide.svr19 3.774
Protein with the highest isoelectric point:
>tr|A0A264W608|A0A264W608_9BACL ChrA protein OS=Tetzosporium hominis OX=2020506 GN=CF394_01000 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPNKK9 pKa = 8.24 RR10 pKa = 11.84 KK11 pKa = 9.6 RR12 pKa = 11.84 SKK14 pKa = 9.83 VHH16 pKa = 5.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 9.66 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.65 GRR39 pKa = 11.84 KK40 pKa = 8.74 VLSAA44 pKa = 4.05
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2916
0
2916
802905
28
2668
275.3
30.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.878 ± 0.051
0.576 ± 0.014
5.271 ± 0.041
7.788 ± 0.053
4.448 ± 0.038
6.84 ± 0.048
2.177 ± 0.026
6.929 ± 0.045
5.865 ± 0.049
9.899 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.728 ± 0.021
3.607 ± 0.034
3.758 ± 0.025
4.2 ± 0.04
4.437 ± 0.039
5.845 ± 0.031
5.892 ± 0.042
7.427 ± 0.039
1.033 ± 0.016
3.402 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here