Arthrobacter phage Judy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bridgettevirus; Arthrobacter virus Judy

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2KGP0|A0A3G2KGP0_9CAUD Uncharacterized protein OS=Arthrobacter phage Judy OX=2419958 GN=65 PE=4 SV=1
MM1 pKa = 7.06NHH3 pKa = 5.9NFKK6 pKa = 10.31EE7 pKa = 4.66VPSTEE12 pKa = 4.19TPDD15 pKa = 5.47DD16 pKa = 4.26GDD18 pKa = 3.41LTLALRR24 pKa = 11.84QRR26 pKa = 11.84AHH28 pKa = 6.04GTLTQQVFGLEE39 pKa = 4.13PYY41 pKa = 10.32IIIRR45 pKa = 11.84PTFAEE50 pKa = 4.12DD51 pKa = 2.73TGAFQVGIEE60 pKa = 4.23TGGGADD66 pKa = 3.69LANVGEE72 pKa = 4.25FLEE75 pKa = 5.32LIAEE79 pKa = 4.78AMQADD84 pKa = 3.84ATKK87 pKa = 10.63EE88 pKa = 3.9NIAAATSGDD97 pKa = 4.31DD98 pKa = 3.79PDD100 pKa = 4.02EE101 pKa = 5.92DD102 pKa = 3.87EE103 pKa = 4.6

Molecular weight: 10.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2KGG8|A0A3G2KGG8_9CAUD Portal protein OS=Arthrobacter phage Judy OX=2419958 GN=3 PE=4 SV=1
MM1 pKa = 7.14YY2 pKa = 9.47PDD4 pKa = 4.58AATAHH9 pKa = 5.55YY10 pKa = 10.87RR11 pKa = 11.84LMQRR15 pKa = 11.84LQTVAVVAGRR25 pKa = 11.84RR26 pKa = 11.84AWSRR30 pKa = 11.84IDD32 pKa = 3.73PGDD35 pKa = 4.0LSGSWMQGIAVVAGIVTTQQEE56 pKa = 4.13RR57 pKa = 11.84AAVAGAGYY65 pKa = 8.22VTDD68 pKa = 4.66ALAAQGEE75 pKa = 4.85YY76 pKa = 10.11VAPEE80 pKa = 4.09AFLNPAAFAGTAADD94 pKa = 3.89GRR96 pKa = 11.84SLSTLLYY103 pKa = 10.91SPVTTVKK110 pKa = 10.37QQLAEE115 pKa = 4.25GASMASALTAGRR127 pKa = 11.84GALDD131 pKa = 3.12MLLRR135 pKa = 11.84VTVADD140 pKa = 3.91AGRR143 pKa = 11.84VASGVNVATRR153 pKa = 11.84QSVGYY158 pKa = 10.63VRR160 pKa = 11.84MLNPPSCPRR169 pKa = 11.84CSVLAGRR176 pKa = 11.84FYY178 pKa = 10.94RR179 pKa = 11.84WNAGFLRR186 pKa = 11.84HH187 pKa = 6.09PKK189 pKa = 9.45CDD191 pKa = 3.72CVHH194 pKa = 5.82VPSKK198 pKa = 11.03GVDD201 pKa = 3.19AAKK204 pKa = 10.62SEE206 pKa = 4.09GLIHH210 pKa = 7.67DD211 pKa = 4.55PYY213 pKa = 11.06EE214 pKa = 4.36YY215 pKa = 10.58FHH217 pKa = 6.93SLSEE221 pKa = 3.98QEE223 pKa = 4.27QNEE226 pKa = 4.75KK227 pKa = 8.4YY228 pKa = 8.81TKK230 pKa = 10.37AGARR234 pKa = 11.84AIRR237 pKa = 11.84DD238 pKa = 3.43GGDD241 pKa = 2.42IFQVVNSQRR250 pKa = 11.84GMSYY254 pKa = 11.34ASISKK259 pKa = 10.33DD260 pKa = 2.84GTRR263 pKa = 11.84RR264 pKa = 11.84GQRR267 pKa = 11.84STSRR271 pKa = 11.84FTTEE275 pKa = 3.17GTTRR279 pKa = 11.84RR280 pKa = 11.84GNFGRR285 pKa = 11.84KK286 pKa = 8.87GRR288 pKa = 11.84LTPEE292 pKa = 5.35AIYY295 pKa = 9.78QQSGSRR301 pKa = 11.84EE302 pKa = 3.65EE303 pKa = 4.1ALRR306 pKa = 11.84LLAANGYY313 pKa = 9.12ILPGGQNPLGSLRR326 pKa = 11.84GQRR329 pKa = 11.84EE330 pKa = 4.09GFGQLGRR337 pKa = 11.84GGTRR341 pKa = 11.84VGARR345 pKa = 11.84EE346 pKa = 3.87AVLEE350 pKa = 3.87ARR352 pKa = 11.84RR353 pKa = 11.84TGVRR357 pKa = 11.84TGARR361 pKa = 11.84ATMTAAEE368 pKa = 4.58LRR370 pKa = 11.84VFDD373 pKa = 5.47ARR375 pKa = 11.84SRR377 pKa = 11.84WDD379 pKa = 3.35QVRR382 pKa = 11.84KK383 pKa = 9.73GVNPFNPKK391 pKa = 10.09RR392 pKa = 11.84PLTPEE397 pKa = 2.95IAARR401 pKa = 11.84VEE403 pKa = 3.8RR404 pKa = 11.84DD405 pKa = 3.2FRR407 pKa = 11.84KK408 pKa = 10.63YY409 pKa = 10.25ILGYY413 pKa = 10.5

Molecular weight: 44.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

13953

30

1298

193.8

21.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.911 ± 0.57

0.953 ± 0.142

6.013 ± 0.174

5.784 ± 0.307

2.559 ± 0.14

8.658 ± 0.358

1.957 ± 0.226

4.307 ± 0.207

4.408 ± 0.308

7.74 ± 0.322

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.114 ± 0.145

3.017 ± 0.19

5.87 ± 0.42

4.286 ± 0.404

6.515 ± 0.414

5.569 ± 0.277

7.131 ± 0.362

6.852 ± 0.33

1.82 ± 0.219

2.537 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski