Roseospira marina

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Roseospira

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4009 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5M6IAI8|A0A5M6IAI8_9PROT GAF domain-containing protein OS=Roseospira marina OX=140057 GN=F1188_11980 PE=3 SV=1
MM1 pKa = 7.37SVLANALRR9 pKa = 11.84TGTAGAVALALGALGTVGAVAADD32 pKa = 4.54LDD34 pKa = 4.02PGKK37 pKa = 9.84PYY39 pKa = 10.97DD40 pKa = 4.14LGRR43 pKa = 11.84DD44 pKa = 3.58ATPEE48 pKa = 3.89EE49 pKa = 4.29VAGWDD54 pKa = 3.55IDD56 pKa = 3.92VLPDD60 pKa = 3.26GTGALVGQGTPAEE73 pKa = 4.43GEE75 pKa = 4.39AIFMAQCAVCHH86 pKa = 6.2GEE88 pKa = 3.77FGEE91 pKa = 4.74GVDD94 pKa = 4.28RR95 pKa = 11.84YY96 pKa = 9.42PVLFGGEE103 pKa = 4.06DD104 pKa = 3.43TLASRR109 pKa = 11.84DD110 pKa = 3.75PVKK113 pKa = 10.28TPGSYY118 pKa = 8.65WPYY121 pKa = 10.86ASTLVDD127 pKa = 3.6YY128 pKa = 10.17IYY130 pKa = 10.93RR131 pKa = 11.84AMPFGQAQTLTPDD144 pKa = 2.89QAYY147 pKa = 10.61ALTAYY152 pKa = 10.57LLYY155 pKa = 10.51INFVLDD161 pKa = 4.56DD162 pKa = 4.12EE163 pKa = 5.76DD164 pKa = 4.84FVLSNEE170 pKa = 4.37TIGAVEE176 pKa = 4.05MPNRR180 pKa = 11.84DD181 pKa = 2.89GFIRR185 pKa = 11.84DD186 pKa = 4.47DD187 pKa = 4.17RR188 pKa = 11.84PDD190 pKa = 3.67AQPEE194 pKa = 4.57TVCMTACDD202 pKa = 3.76VPTQVVGRR210 pKa = 11.84ARR212 pKa = 11.84IVDD215 pKa = 3.66VTPDD219 pKa = 3.64DD220 pKa = 5.24DD221 pKa = 3.92EE222 pKa = 4.54TAGLSLEE229 pKa = 4.23

Molecular weight: 24.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5M6IEH4|A0A5M6IEH4_9PROT Tripartite tricarboxylate transporter permease OS=Roseospira marina OX=140057 GN=F1188_04940 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.27RR3 pKa = 11.84PYY5 pKa = 10.01QPSKK9 pKa = 10.19LVRR12 pKa = 11.84KK13 pKa = 8.95RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.64GFRR19 pKa = 11.84SRR21 pKa = 11.84SATVGGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4009

0

4009

1330878

29

2901

332.0

35.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.232 ± 0.064

0.89 ± 0.01

6.348 ± 0.035

5.421 ± 0.03

3.163 ± 0.022

8.905 ± 0.036

2.189 ± 0.019

4.293 ± 0.028

2.24 ± 0.027

10.215 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.445 ± 0.019

2.145 ± 0.02

5.89 ± 0.034

2.784 ± 0.018

7.809 ± 0.042

4.726 ± 0.023

5.985 ± 0.032

7.975 ± 0.03

1.365 ± 0.016

1.978 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski